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The map label for this gene is amtB [C]

Identifier: 75909164

GI number: 75909164

Start: 3660210

End: 3661676

Strand: Direct

Name: amtB [C]

Synonym: Ava_2954

Alternate gene names: 75909164

Gene position: 3660210-3661676 (Clockwise)

Preceding gene: 75909163

Following gene: 75909165

Centisome position: 57.5

GC content: 44.79

Gene sequence:

>1467_bases
ATGAATAGGCATATTCAACCCTGGCAACGCCTATTGGTGTTAGCGATTGGCTCGATGGTATTTGCTGTTTTTGCACCGAC
AATTGTGCAAGCGGCAAATACTCCTACCCTAGAATCTTTATCAGAAACTACAATCAAACTGCAAATTTCCATTGATACTA
CTTGGGTACTACTTTCTGGGTTTTTAGTATTTTTCATGCAAACCGGCTTCGCCATGCTAGAAGCTGGTTTAGTTCGCCAA
AGAAGTGTAGTTAATACCCTACTAGAAAATTTCATTGACGCTGCTGTCACAGTCTTGGCATGGTGGGCAGTGGGTTTTGG
TATCGCATTTGGTACAAGTGCAGGTGGTTTGTTTGGTATAGATACATTCTTTCTCAACCAGTTACCGGGTGCTGATGGTA
GCTACCCATTGGGCGCACCTGGTTCTACAGCTGCTATCAACACCTATACTTTGTTCTTTTTCCAGTTTGCCTTTGCTGCT
ACTGCCAGTACCATCACTACTGGTTCAATGGCTGGCAGAACCGACTTTATAGGTGACTTAATTTATAGTGCGATTATGGG
TGCTATTAGCTACCCAATTATCGTGCATTGGGCTTGGAACTCCAATGGCTGGTTAGGCAAACTCAGTTACCATGATTTCG
CCGGTGGTTCAATTGTTCATACCGTAGGTGGCTGGACAGCACTAGTTGGGGCATATTTGCTTGGGCCTCGTCCCGACCGT
CCTCCTTGGGGAAAACTACCACCAGCCCATAATTTAGCCTTGGCAACTTTGGGAACGATGATTCTCTGGTTTGGTTGGTA
CGGGTTTAACCCTGGTTCAACCCTTGGTACTGCTAATCCAGGGTTGATTGGGTTAGTAACTATTAACACAACACTAGCAG
CAGGTGCAGGCGCACTAACAGCATTAATCTTTCTGTATGTTCGTACAGGTAAGTGGGATTTAGTTTACTGTCTCAATGGT
TCTCTAGCTGGTTTAGTCGCAATTACAGCACCTTGCGCTTATGTGGCTCCTTGGGCTTCTGTACTAATTGGTTTCACGGG
GGGAATTTCGGTTGTATTAGGAGTGAGTTTAATTGAGTCACTCCACATCGATGACCCAGTGGGAGCATTTTCCGTCCACG
GTATCAGTGGCATGATGGGTACTCTCTCCATAGGTTTTTTAGGACAAGCGGAACTCACCCTTAATCAAAAAGCAGGGCTA
TTTTTAGGTGGTGGGTTTGATTTGCTGGGTATCCAAATGCTGGGGATAGTGGCGATTACGGTTTTCACCGTCGCTTTTGC
CTTTCTGATGTATGGTGGCCTCAAAGCTGTGGGATACTTACGTGTTAATGCTGAAGCAGATAGTATCGGCATTGATGCTT
ATGAACATGGTGCTTCAGTGTGGCCTGATATTTACCCAGTTGAAGAGGTAAGCAAACTACAAGACCAGACAAAAATTTCT
GAAAATAAAACTTTAGAGGAGCAATAA

Upstream 100 bases:

>100_bases
ACCAAATCTTGTCTACATTATTTAGTTCACCTAACCACCAAGGGTAAGTCTGCTACTACTAACATCAATTATGGCTATAA
ACACAGGAAAAAATAAGCTG

Downstream 100 bases:

>100_bases
AATGGCCAATATTTTCAATATTTTATCTGATCAATCTTTACAGAAGTTTCTTGGAAATGATATTGACAATGTGAGGCGAT
CGCCCCTTTAACTCGTCAAA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MNRHIQPWQRLLVLAIGSMVFAVFAPTIVQAANTPTLESLSETTIKLQISIDTTWVLLSGFLVFFMQTGFAMLEAGLVRQ
RSVVNTLLENFIDAAVTVLAWWAVGFGIAFGTSAGGLFGIDTFFLNQLPGADGSYPLGAPGSTAAINTYTLFFFQFAFAA
TASTITTGSMAGRTDFIGDLIYSAIMGAISYPIIVHWAWNSNGWLGKLSYHDFAGGSIVHTVGGWTALVGAYLLGPRPDR
PPWGKLPPAHNLALATLGTMILWFGWYGFNPGSTLGTANPGLIGLVTINTTLAAGAGALTALIFLYVRTGKWDLVYCLNG
SLAGLVAITAPCAYVAPWASVLIGFTGGISVVLGVSLIESLHIDDPVGAFSVHGISGMMGTLSIGFLGQAELTLNQKAGL
FLGGGFDLLGIQMLGIVAITVFTVAFAFLMYGGLKAVGYLRVNAEADSIGIDAYEHGASVWPDIYPVEEVSKLQDQTKIS
ENKTLEEQ

Sequences:

>Translated_488_residues
MNRHIQPWQRLLVLAIGSMVFAVFAPTIVQAANTPTLESLSETTIKLQISIDTTWVLLSGFLVFFMQTGFAMLEAGLVRQ
RSVVNTLLENFIDAAVTVLAWWAVGFGIAFGTSAGGLFGIDTFFLNQLPGADGSYPLGAPGSTAAINTYTLFFFQFAFAA
TASTITTGSMAGRTDFIGDLIYSAIMGAISYPIIVHWAWNSNGWLGKLSYHDFAGGSIVHTVGGWTALVGAYLLGPRPDR
PPWGKLPPAHNLALATLGTMILWFGWYGFNPGSTLGTANPGLIGLVTINTTLAAGAGALTALIFLYVRTGKWDLVYCLNG
SLAGLVAITAPCAYVAPWASVLIGFTGGISVVLGVSLIESLHIDDPVGAFSVHGISGMMGTLSIGFLGQAELTLNQKAGL
FLGGGFDLLGIQMLGIVAITVFTVAFAFLMYGGLKAVGYLRVNAEADSIGIDAYEHGASVWPDIYPVEEVSKLQDQTKIS
ENKTLEEQ
>Mature_488_residues
MNRHIQPWQRLLVLAIGSMVFAVFAPTIVQAANTPTLESLSETTIKLQISIDTTWVLLSGFLVFFMQTGFAMLEAGLVRQ
RSVVNTLLENFIDAAVTVLAWWAVGFGIAFGTSAGGLFGIDTFFLNQLPGADGSYPLGAPGSTAAINTYTLFFFQFAFAA
TASTITTGSMAGRTDFIGDLIYSAIMGAISYPIIVHWAWNSNGWLGKLSYHDFAGGSIVHTVGGWTALVGAYLLGPRPDR
PPWGKLPPAHNLALATLGTMILWFGWYGFNPGSTLGTANPGLIGLVTINTTLAAGAGALTALIFLYVRTGKWDLVYCLNG
SLAGLVAITAPCAYVAPWASVLIGFTGGISVVLGVSLIESLHIDDPVGAFSVHGISGMMGTLSIGFLGQAELTLNQKAGL
FLGGGFDLLGIQMLGIVAITVFTVAFAFLMYGGLKAVGYLRVNAEADSIGIDAYEHGASVWPDIYPVEEVSKLQDQTKIS
ENKTLEEQ

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=413, Percent_Identity=30.0242130750605, Blast_Score=153, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17550182, Length=441, Percent_Identity=35.3741496598639, Blast_Score=248, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI17550180, Length=494, Percent_Identity=29.9595141700405, Blast_Score=235, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI32564042, Length=420, Percent_Identity=32.1428571428571, Blast_Score=205, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI133931100, Length=448, Percent_Identity=30.8035714285714, Blast_Score=185, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17563228, Length=242, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6324187, Length=444, Percent_Identity=31.0810810810811, Blast_Score=168, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6321559, Length=458, Percent_Identity=26.2008733624454, Blast_Score=133, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6325396, Length=418, Percent_Identity=27.511961722488, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI161078314, Length=413, Percent_Identity=35.1089588377724, Blast_Score=216, Evalue=4e-56,
Organism=Drosophila melanogaster, GI21355411, Length=235, Percent_Identity=28.936170212766, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 51662; Mature: 51662

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRHIQPWQRLLVLAIGSMVFAVFAPTIVQAANTPTLESLSETTIKLQISIDTTWVLLSG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEEEEEECHHHHHHHH
FLVFFMQTGFAMLEAGLVRQRSVVNTLLENFIDAAVTVLAWWAVGFGIAFGTSAGGLFGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
DTFFLNQLPGADGSYPLGAPGSTAAINTYTLFFFQFAFAATASTITTGSMAGRTDFIGDL
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHH
IYSAIMGAISYPIIVHWAWNSNGWLGKLSYHDFAGGSIVHTVGGWTALVGAYLLGPRPDR
HHHHHHHHHCCCEEEEEEECCCCCEEECCCCCCCCCCEEEECCHHHHHHHHHHCCCCCCC
PPWGKLPPAHNLALATLGTMILWFGWYGFNPGSTLGTANPGLIGLVTINTTLAAGAGALT
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCHHHCCHHHHH
ALIFLYVRTGKWDLVYCLNGSLAGLVAITAPCAYVAPWASVLIGFTGGISVVLGVSLIES
HHHHHHHCCCCEEEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LHIDDPVGAFSVHGISGMMGTLSIGFLGQAELTLNQKAGLFLGGGFDLLGIQMLGIVAIT
HCCCCCCCCEEECCHHHHHHHHEECCCCCEEEEEECCCCEEEECCHHHHHHHHHHHHHHH
VFTVAFAFLMYGGLKAVGYLRVNAEADSIGIDAYEHGASVWPDIYPVEEVSKLQDQTKIS
HHHHHHHHHHHCCHHEEEEEEEECCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHC
ENKTLEEQ
CCCCCCCC
>Mature Secondary Structure
MNRHIQPWQRLLVLAIGSMVFAVFAPTIVQAANTPTLESLSETTIKLQISIDTTWVLLSG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEEEEEECHHHHHHHH
FLVFFMQTGFAMLEAGLVRQRSVVNTLLENFIDAAVTVLAWWAVGFGIAFGTSAGGLFGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
DTFFLNQLPGADGSYPLGAPGSTAAINTYTLFFFQFAFAATASTITTGSMAGRTDFIGDL
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHH
IYSAIMGAISYPIIVHWAWNSNGWLGKLSYHDFAGGSIVHTVGGWTALVGAYLLGPRPDR
HHHHHHHHHCCCEEEEEEECCCCCEEECCCCCCCCCCEEEECCHHHHHHHHHHCCCCCCC
PPWGKLPPAHNLALATLGTMILWFGWYGFNPGSTLGTANPGLIGLVTINTTLAAGAGALT
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCHHHCCHHHHH
ALIFLYVRTGKWDLVYCLNGSLAGLVAITAPCAYVAPWASVLIGFTGGISVVLGVSLIES
HHHHHHHCCCCEEEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LHIDDPVGAFSVHGISGMMGTLSIGFLGQAELTLNQKAGLFLGGGFDLLGIQMLGIVAIT
HCCCCCCCCEEECCHHHHHHHHEECCCCCEEEEEECCCCEEEECCHHHHHHHHHHHHHHH
VFTVAFAFLMYGGLKAVGYLRVNAEADSIGIDAYEHGASVWPDIYPVEEVSKLQDQTKIS
HHHHHHHHHHHCCHHEEEEEEEECCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHC
ENKTLEEQ
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]