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The map label for this gene is nrgA [H]

Identifier: 75909166

GI number: 75909166

Start: 3663990

End: 3665402

Strand: Direct

Name: nrgA [H]

Synonym: Ava_2956

Alternate gene names: 75909166

Gene position: 3663990-3665402 (Clockwise)

Preceding gene: 75909165

Following gene: 75909167

Centisome position: 57.56

GC content: 48.2

Gene sequence:

>1413_bases
GTGTTTCAGAAAATTTTGATAACTGGGGTGATTACCCTACTACTGCTGACAATTCCTGTTATGGGTAGTGCTTGGGCGCA
AGTCCCAGCAACGGCTCCACCTGCTGATACAGGAGATACAGCATTTATGCTGATTTCATCAGCCCTAGTCTTGCTGATGA
CACCAGGATTGGCATTTTTCTACGGTGGATTTGTACGATCGCGCAATATTCTCAATACATTGATGATGAGTTTTGTATTG
ATGGCGATCGTCGGCGTTACTTGGGTTTTATGGGGTTATAGTCTGTCATTTGCGCCAGGACTGCCATTTATTGGTGGTTT
ACAGTGGTTTGGGTTAAACGGTGTGGGGCTAGAAACCACAGGTTATCTGCAAGGTTCAGAGCCGACAAATGTGGTTTCTT
ATGCCGGGACGATTCCCCATCAGGCATACATGATATATCAAGCCATGTTTGCCATTATCACCCCAGCCTTAATTTCTGGA
GCGATCGCCGAACGGATGAGTTTCCGTGCCTATTGCCTATTTGTGCTACTGTGGTCAACCTTTGTTTACACGCCTCTAGC
CCACATGGTTTGGGCAAAAGGTGGATTATTAGGTTTGTACGGTGGCTTAGGCGCGCTCGACTTCGCCGGCGGTACAGTAG
TTCATATTAGCTCTGGGGTTTCTGCCCTCGTGGCCGCAATAGTCCTGGGACCGAGGAAAACCCATCCCGATCGCCTCAGC
CCTCCCCACAACGTAACATTTATTTTGTTGGGTGCTGGTTTGCTATGGTTTGGCTGGTTCGGTTTTAATGCTGGGAGTGC
CTTATCTGTTGCTAGTAGCACTTCTGGCAGTTTCATTACTAATCCAGCCACAACTGCCTTTGTTGCCACCAATACCTCAG
CCGCAGCTGGGGCTTTAATGTGGCTAATTCTAGAAGGAGTATTACGCGGTAAACCCACCGCCGTAGGTGCAGCCACAGGC
GCAGTTGCCGGTTTAGTAGGGATTACCCCAGCCGCCGGCTTTGTCACCCCTCTAGCGTCAATTTTGATAGGTTGTATCAC
AGCTTTTGTTTGCTTCTATGCCGTAAGTTTCAAACACAAGTTAAATGTTGACGATGCCTTAGATACCTACCCGGTGCATG
GTGTTGGTGGAACAGTTGGCGCAATTCTCACAGCCTTGTTTGCCACCGCCGAAGTTAACTCAGGCGGTAAAAACGGCGTA
CTCAAGGGTAATTTTGGTGAACTAGGTGTGGAACTGGCCGCCATTGTCATCGCCTATGTAATTGCAGCAGTTGGCACTTG
GATAATTTTGAAGATCATTGATGCTAGCGTTGGCTTACGGGTGAAAGAAGAAGCCGAATATCAAGGTTTGGATATCAACG
AACACGGAGAAGAAGCATACAACTCCGAGTTTAGCGATCGCATCGTACCGTAA

Upstream 100 bases:

>100_bases
ATAAATCAGAGTAGCTTGTAAGACAAAAATCTTTGGCTAAAAGTAATGAAATGTAACTAAGTATACAAGGTAACTGATCA
TCAGGGAGTAAGAAATAAAC

Downstream 100 bases:

>100_bases
AAATTTAGGCATAGCCCAAATTTGACAACTGACTAATGACTAATGACTAATGACTAATACCGATTTAAAATCTGAGTCAA
ATCATTGGTAGATGTCGCTA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MFQKILITGVITLLLLTIPVMGSAWAQVPATAPPADTGDTAFMLISSALVLLMTPGLAFFYGGFVRSRNILNTLMMSFVL
MAIVGVTWVLWGYSLSFAPGLPFIGGLQWFGLNGVGLETTGYLQGSEPTNVVSYAGTIPHQAYMIYQAMFAIITPALISG
AIAERMSFRAYCLFVLLWSTFVYTPLAHMVWAKGGLLGLYGGLGALDFAGGTVVHISSGVSALVAAIVLGPRKTHPDRLS
PPHNVTFILLGAGLLWFGWFGFNAGSALSVASSTSGSFITNPATTAFVATNTSAAAGALMWLILEGVLRGKPTAVGAATG
AVAGLVGITPAAGFVTPLASILIGCITAFVCFYAVSFKHKLNVDDALDTYPVHGVGGTVGAILTALFATAEVNSGGKNGV
LKGNFGELGVELAAIVIAYVIAAVGTWIILKIIDASVGLRVKEEAEYQGLDINEHGEEAYNSEFSDRIVP

Sequences:

>Translated_470_residues
MFQKILITGVITLLLLTIPVMGSAWAQVPATAPPADTGDTAFMLISSALVLLMTPGLAFFYGGFVRSRNILNTLMMSFVL
MAIVGVTWVLWGYSLSFAPGLPFIGGLQWFGLNGVGLETTGYLQGSEPTNVVSYAGTIPHQAYMIYQAMFAIITPALISG
AIAERMSFRAYCLFVLLWSTFVYTPLAHMVWAKGGLLGLYGGLGALDFAGGTVVHISSGVSALVAAIVLGPRKTHPDRLS
PPHNVTFILLGAGLLWFGWFGFNAGSALSVASSTSGSFITNPATTAFVATNTSAAAGALMWLILEGVLRGKPTAVGAATG
AVAGLVGITPAAGFVTPLASILIGCITAFVCFYAVSFKHKLNVDDALDTYPVHGVGGTVGAILTALFATAEVNSGGKNGV
LKGNFGELGVELAAIVIAYVIAAVGTWIILKIIDASVGLRVKEEAEYQGLDINEHGEEAYNSEFSDRIVP
>Mature_470_residues
MFQKILITGVITLLLLTIPVMGSAWAQVPATAPPADTGDTAFMLISSALVLLMTPGLAFFYGGFVRSRNILNTLMMSFVL
MAIVGVTWVLWGYSLSFAPGLPFIGGLQWFGLNGVGLETTGYLQGSEPTNVVSYAGTIPHQAYMIYQAMFAIITPALISG
AIAERMSFRAYCLFVLLWSTFVYTPLAHMVWAKGGLLGLYGGLGALDFAGGTVVHISSGVSALVAAIVLGPRKTHPDRLS
PPHNVTFILLGAGLLWFGWFGFNAGSALSVASSTSGSFITNPATTAFVATNTSAAAGALMWLILEGVLRGKPTAVGAATG
AVAGLVGITPAAGFVTPLASILIGCITAFVCFYAVSFKHKLNVDDALDTYPVHGVGGTVGAILTALFATAEVNSGGKNGV
LKGNFGELGVELAAIVIAYVIAAVGTWIILKIIDASVGLRVKEEAEYQGLDINEHGEEAYNSEFSDRIVP

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=438, Percent_Identity=42.9223744292237, Blast_Score=308, Evalue=7e-85,
Organism=Caenorhabditis elegans, GI17550182, Length=449, Percent_Identity=29.6213808463252, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI133931100, Length=438, Percent_Identity=27.3972602739726, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17550180, Length=448, Percent_Identity=28.125, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI32564042, Length=439, Percent_Identity=25.9681093394077, Blast_Score=115, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6324187, Length=452, Percent_Identity=36.0619469026549, Blast_Score=236, Evalue=7e-63,
Organism=Saccharomyces cerevisiae, GI6325396, Length=437, Percent_Identity=35.9267734553776, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6321559, Length=448, Percent_Identity=33.7053571428571, Blast_Score=214, Evalue=2e-56,
Organism=Drosophila melanogaster, GI161078314, Length=431, Percent_Identity=29.2343387470998, Blast_Score=129, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 49030; Mature: 49030

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQKILITGVITLLLLTIPVMGSAWAQVPATAPPADTGDTAFMLISSALVLLMTPGLAFF
CCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHH
YGGFVRSRNILNTLMMSFVLMAIVGVTWVLWGYSLSFAPGLPFIGGLQWFGLNGVGLETT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCC
GYLQGSEPTNVVSYAGTIPHQAYMIYQAMFAIITPALISGAIAERMSFRAYCLFVLLWST
CCCCCCCCCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVYTPLAHMVWAKGGLLGLYGGLGALDFAGGTVVHISSGVSALVAAIVLGPRKTHPDRLS
HHHHHHHHHHHHCCCHHHHHCCCCCHHCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCC
PPHNVTFILLGAGLLWFGWFGFNAGSALSVASSTSGSFITNPATTAFVATNTSAAAGALM
CCCCEEEEEEHHHHHHHHHHCCCCCCCEEEECCCCCCEEECCCCEEEEEECCCHHHHHHH
WLILEGVLRGKPTAVGAATGAVAGLVGITPAAGFVTPLASILIGCITAFVCFYAVSFKHK
HHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LNVDDALDTYPVHGVGGTVGAILTALFATAEVNSGGKNGVLKGNFGELGVELAAIVIAYV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHHHHHHHHHHH
IAAVGTWIILKIIDASVGLRVKEEAEYQGLDINEHGEEAYNSEFSDRIVP
HHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCHHHCCCCCCCCCC
>Mature Secondary Structure
MFQKILITGVITLLLLTIPVMGSAWAQVPATAPPADTGDTAFMLISSALVLLMTPGLAFF
CCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHH
YGGFVRSRNILNTLMMSFVLMAIVGVTWVLWGYSLSFAPGLPFIGGLQWFGLNGVGLETT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCC
GYLQGSEPTNVVSYAGTIPHQAYMIYQAMFAIITPALISGAIAERMSFRAYCLFVLLWST
CCCCCCCCCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVYTPLAHMVWAKGGLLGLYGGLGALDFAGGTVVHISSGVSALVAAIVLGPRKTHPDRLS
HHHHHHHHHHHHCCCHHHHHCCCCCHHCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCC
PPHNVTFILLGAGLLWFGWFGFNAGSALSVASSTSGSFITNPATTAFVATNTSAAAGALM
CCCCEEEEEEHHHHHHHHHHCCCCCCCEEEECCCCCCEEECCCCEEEEEECCCHHHHHHH
WLILEGVLRGKPTAVGAATGAVAGLVGITPAAGFVTPLASILIGCITAFVCFYAVSFKHK
HHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LNVDDALDTYPVHGVGGTVGAILTALFATAEVNSGGKNGVLKGNFGELGVELAAIVIAYV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHHHHHHHHHHH
IAAVGTWIILKIIDASVGLRVKEEAEYQGLDINEHGEEAYNSEFSDRIVP
HHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]