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The map label for this gene is rfbB [H]

Identifier: 75908852

GI number: 75908852

Start: 3267459

End: 3268529

Strand: Direct

Name: rfbB [H]

Synonym: Ava_2639

Alternate gene names: 75908852

Gene position: 3267459-3268529 (Clockwise)

Preceding gene: 75908849

Following gene: 75908853

Centisome position: 51.33

GC content: 43.6

Gene sequence:

>1071_bases
ATGATACAAAATGATACAGAACTATTGACAACATCTGGTGCGGCTGTACCTATTCTGATTACGGGTGGTGCAGGCTTTAT
TGGCTCCAATTTCGTCCATCATTGGTATGAACAGTACCCAGGCGATCGCATAATTGTTTTGGATGCGCTCACCTATGCAG
GGAATCGCCAAAATTTAGCAGATATAGAAGGAAAAGCAAATTTAAGATTTGTCAAGGGAGATATAGGTGATCGCGCTCTC
ATTGATCAGCTACTAGAGGAAGAAAAGATTCAGGCGATCGCCCACTTTGCAGCTGAATCTCACGTTGATCGCTCAATTGT
CGCGCCAGATGCTTTCATTCAGACCAATGTTGTAGGTACATTTACTTTATTAGAAGCCTTTCGCCATCACTGGACAAAAC
AAGGCAAACCTGCTAACTACCGCTTTCTCCACGTCTCTACAGATGAAGTTTACGGCAGCCTTGAACTAGATGATCCAGCT
TTTACAGAAACAACTCCTTACGCCCCCAACAGTCCCTATTCCGCCTCTAAAGCAGGTAGTGATCATCTAGCACGAGCTTA
TTACCACACCTACGGTTTACCAACCTTAATTACAAATTGCTCCAATAACTACGGCCCCTATCACTTCCCCGAAAAATTAA
TTCCCCTAATATGCCTCAATATTCTCTTAGGTAAACCTCTACCTATCTATGGAGATGGGTTAAATATCCGTGATTGGTTA
TATGTTGAAGACCATTGTCGTGCTTTAGATATTGTCATTCATCAGGGTAAACCAGGAGAAACCTACAACATTGGCGGTAA
TAACGAAATCAAAAACATTGACCTTGTTCAGATGATCTGTGAGTTAATGGACGAATTAGCCCCTGATTTACCCGTCTCTC
CCGCCAGTAAACTCATTACCTTCGTCAAAGACCGCCCCGGACACGATCGCCGTTATGCGATCGATGCGACAAAAATCAAA
ACAGAATTAGGTTGGGAACCCCAACAAACAATCTCGACTGGATTACGCCACACCATCCAGTGGTATCTAACTCATCGCCA
TTGGTGGGAAGCACTTTTACCAAAGGAGTAG

Upstream 100 bases:

>100_bases
CAGCCTAAAGATGGAAGATTTTGTCAACGGTAAACTTTATTGATATTGTTTCTTGTGAATTCTGCGGTGTTTTCTCCCAA
ACTAAAGCAAAAATAAATCC

Downstream 100 bases:

>100_bases
GTCAAGATTATCAGGGAAAAATCATGTTCACCAACTTTGAACAAACCATAGTTGATACAACAGCAGCTCAAATCAATCTA
GTCAAAGGAGGTCAAGGCGC

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MIQNDTELLTTSGAAVPILITGGAGFIGSNFVHHWYEQYPGDRIIVLDALTYAGNRQNLADIEGKANLRFVKGDIGDRAL
IDQLLEEEKIQAIAHFAAESHVDRSIVAPDAFIQTNVVGTFTLLEAFRHHWTKQGKPANYRFLHVSTDEVYGSLELDDPA
FTETTPYAPNSPYSASKAGSDHLARAYYHTYGLPTLITNCSNNYGPYHFPEKLIPLICLNILLGKPLPIYGDGLNIRDWL
YVEDHCRALDIVIHQGKPGETYNIGGNNEIKNIDLVQMICELMDELAPDLPVSPASKLITFVKDRPGHDRRYAIDATKIK
TELGWEPQQTISTGLRHTIQWYLTHRHWWEALLPKE

Sequences:

>Translated_356_residues
MIQNDTELLTTSGAAVPILITGGAGFIGSNFVHHWYEQYPGDRIIVLDALTYAGNRQNLADIEGKANLRFVKGDIGDRAL
IDQLLEEEKIQAIAHFAAESHVDRSIVAPDAFIQTNVVGTFTLLEAFRHHWTKQGKPANYRFLHVSTDEVYGSLELDDPA
FTETTPYAPNSPYSASKAGSDHLARAYYHTYGLPTLITNCSNNYGPYHFPEKLIPLICLNILLGKPLPIYGDGLNIRDWL
YVEDHCRALDIVIHQGKPGETYNIGGNNEIKNIDLVQMICELMDELAPDLPVSPASKLITFVKDRPGHDRRYAIDATKIK
TELGWEPQQTISTGLRHTIQWYLTHRHWWEALLPKE
>Mature_356_residues
MIQNDTELLTTSGAAVPILITGGAGFIGSNFVHHWYEQYPGDRIIVLDALTYAGNRQNLADIEGKANLRFVKGDIGDRAL
IDQLLEEEKIQAIAHFAAESHVDRSIVAPDAFIQTNVVGTFTLLEAFRHHWTKQGKPANYRFLHVSTDEVYGSLELDDPA
FTETTPYAPNSPYSASKAGSDHLARAYYHTYGLPTLITNCSNNYGPYHFPEKLIPLICLNILLGKPLPIYGDGLNIRDWL
YVEDHCRALDIVIHQGKPGETYNIGGNNEIKNIDLVQMICELMDELAPDLPVSPASKLITFVKDRPGHDRRYAIDATKIK
TELGWEPQQTISTGLRHTIQWYLTHRHWWEALLPKE

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=339, Percent_Identity=38.9380530973451, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI42516563, Length=330, Percent_Identity=24.8484848484848, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI4504031, Length=341, Percent_Identity=23.7536656891496, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI48994969, Length=339, Percent_Identity=61.0619469026549, Blast_Score=417, Evalue=1e-118,
Organism=Escherichia coli, GI1788353, Length=343, Percent_Identity=57.1428571428571, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1786974, Length=362, Percent_Identity=28.7292817679558, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788366, Length=284, Percent_Identity=25.3521126760563, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17568069, Length=336, Percent_Identity=37.5, Blast_Score=219, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI115532424, Length=334, Percent_Identity=33.5329341317365, Blast_Score=176, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17539532, Length=330, Percent_Identity=24.5454545454545, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17507723, Length=281, Percent_Identity=24.9110320284698, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI133901786, Length=281, Percent_Identity=24.5551601423488, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17539422, Length=281, Percent_Identity=24.5551601423488, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI133901788, Length=281, Percent_Identity=24.5551601423488, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI133901790, Length=281, Percent_Identity=24.5551601423488, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17539424, Length=281, Percent_Identity=24.5551601423488, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=264, Percent_Identity=25.3787878787879, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21356223, Length=330, Percent_Identity=26.969696969697, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19923002, Length=359, Percent_Identity=26.7409470752089, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40044; Mature: 40044

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQNDTELLTTSGAAVPILITGGAGFIGSNFVHHWYEQYPGDRIIVLDALTYAGNRQNLA
CCCCCCCEEEECCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCH
DIEGKANLRFVKGDIGDRALIDQLLEEEKIQAIAHFAAESHVDRSIVAPDAFIQTNVVGT
HCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHCHHHH
FTLLEAFRHHWTKQGKPANYRFLHVSTDEVYGSLELDDPAFTETTPYAPNSPYSASKAGS
HHHHHHHHHHHHCCCCCCCEEEEEEEHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
DHLARAYYHTYGLPTLITNCSNNYGPYHFPEKLIPLICLNILLGKPLPIYGDGLNIRDWL
HHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCEEE
YVEDHCRALDIVIHQGKPGETYNIGGNNEIKNIDLVQMICELMDELAPDLPVSPASKLIT
EECCCCCEEEEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
FVKDRPGHDRRYAIDATKIKTELGWEPQQTISTGLRHTIQWYLTHRHWWEALLPKE
HHHCCCCCCCEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIQNDTELLTTSGAAVPILITGGAGFIGSNFVHHWYEQYPGDRIIVLDALTYAGNRQNLA
CCCCCCCEEEECCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCH
DIEGKANLRFVKGDIGDRALIDQLLEEEKIQAIAHFAAESHVDRSIVAPDAFIQTNVVGT
HCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHCHHHH
FTLLEAFRHHWTKQGKPANYRFLHVSTDEVYGSLELDDPAFTETTPYAPNSPYSASKAGS
HHHHHHHHHHHHCCCCCCCEEEEEEEHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
DHLARAYYHTYGLPTLITNCSNNYGPYHFPEKLIPLICLNILLGKPLPIYGDGLNIRDWL
HHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCEEE
YVEDHCRALDIVIHQGKPGETYNIGGNNEIKNIDLVQMICELMDELAPDLPVSPASKLIT
EECCCCCEEEEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
FVKDRPGHDRRYAIDATKIKTELGWEPQQTISTGLRHTIQWYLTHRHWWEALLPKE
HHHCCCCCCCEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]