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The map label for this gene is eda [H]

Identifier: 75908662

GI number: 75908662

Start: 3039241

End: 3039882

Strand: Direct

Name: eda [H]

Synonym: Ava_2448

Alternate gene names: 75908662

Gene position: 3039241-3039882 (Clockwise)

Preceding gene: 75908660

Following gene: 75908663

Centisome position: 47.74

GC content: 45.48

Gene sequence:

>642_bases
ATGTCTACTCAAGATTGGTTATCACAGTTGCAAAAACAGCGAGCGATCGCAGTCATCCGCGCCCCGAAACTGGAATTAGG
GTTGCAAATGGCTCTGGCTGTAGCTTCTGGGGGAATACAGCTCATTGAAATTACCTGGAATAGCGATCGCCCAGGGGAAT
TAATCACCAAATTACGTGAGCAGTTACCGGAATGTATTATTGGTACTGGCACATTATTTAATTTGCAGCAACTAGAAGAA
GCGATCGCCTGTGGGGCAGAATTTCTATTTACGCCTCATGTTGACTCAGTCATGATCAAAGCATCTGTCATCAAAAACAT
ACCCATCATTCCCGGCGCACTCACCCCTACGGAAATTGTTAATGCTTGGGCTAATGGTGCAAGTTGTGTCAAAGTATTCC
CCGTGCAAGCTTTAGGGGGGGCTGATTATATTAAAAGTCTGCAAGGGCCTTTAGGTCATATTCCCTTAATTCCCACCGGG
GGGGTAACCCTGGAAAATGCCAAAAGACTAATCCAAGCAGGTGCGATCGCCGTTGGTTTAGGTGGAGAGTTATTTCCTCA
GCAACTAGTGAAAGCGAAAAATTGGCAAGCGATCGCCCAATTAGCCAGGAACTTAACTCAAGAACTAAGCATTATAACCT
AA

Upstream 100 bases:

>100_bases
TATACCCTTCTCCAAACCCTTGATCTTTCGTTTTCATGCGTAAGTCCTATATATATGTCAGTCTGGTAATTGAGAATCGA
ACGCTTCTGCCTCCGTTACA

Downstream 100 bases:

>100_bases
AGCTTGGTAATAAGGATCAAAAATAAGTTTCACTCTCAAACAAAAAAGATGCCTTTCACCTATACCCCTACACCCTTAAA
CCCCTACACCCTTAAATTTT

Product: keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MSTQDWLSQLQKQRAIAVIRAPKLELGLQMALAVASGGIQLIEITWNSDRPGELITKLREQLPECIIGTGTLFNLQQLEE
AIACGAEFLFTPHVDSVMIKASVIKNIPIIPGALTPTEIVNAWANGASCVKVFPVQALGGADYIKSLQGPLGHIPLIPTG
GVTLENAKRLIQAGAIAVGLGGELFPQQLVKAKNWQAIAQLARNLTQELSIIT

Sequences:

>Translated_213_residues
MSTQDWLSQLQKQRAIAVIRAPKLELGLQMALAVASGGIQLIEITWNSDRPGELITKLREQLPECIIGTGTLFNLQQLEE
AIACGAEFLFTPHVDSVMIKASVIKNIPIIPGALTPTEIVNAWANGASCVKVFPVQALGGADYIKSLQGPLGHIPLIPTG
GVTLENAKRLIQAGAIAVGLGGELFPQQLVKAKNWQAIAQLARNLTQELSIIT
>Mature_212_residues
STQDWLSQLQKQRAIAVIRAPKLELGLQMALAVASGGIQLIEITWNSDRPGELITKLREQLPECIIGTGTLFNLQQLEEA
IACGAEFLFTPHVDSVMIKASVIKNIPIIPGALTPTEIVNAWANGASCVKVFPVQALGGADYIKSLQGPLGHIPLIPTGG
VTLENAKRLIQAGAIAVGLGGELFPQQLVKAKNWQAIAQLARNLTQELSIIT

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=184, Percent_Identity=30.4347826086957, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI48994954, Length=172, Percent_Identity=31.9767441860465, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 22759; Mature: 22628

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQDWLSQLQKQRAIAVIRAPKLELGLQMALAVASGGIQLIEITWNSDRPGELITKLRE
CCCHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHH
QLPECIIGTGTLFNLQQLEEAIACGAEFLFTPHVDSVMIKASVIKNIPIIPGALTPTEIV
HHHHHEECCCCCHHHHHHHHHHHCCHHHEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHH
NAWANGASCVKVFPVQALGGADYIKSLQGPLGHIPLIPTGGVTLENAKRLIQAGAIAVGL
HHHCCCCCEEEEEEHHHCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEC
GGELFPQQLVKAKNWQAIAQLARNLTQELSIIT
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STQDWLSQLQKQRAIAVIRAPKLELGLQMALAVASGGIQLIEITWNSDRPGELITKLRE
CCHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHH
QLPECIIGTGTLFNLQQLEEAIACGAEFLFTPHVDSVMIKASVIKNIPIIPGALTPTEIV
HHHHHEECCCCCHHHHHHHHHHHCCHHHEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHH
NAWANGASCVKVFPVQALGGADYIKSLQGPLGHIPLIPTGGVTLENAKRLIQAGAIAVGL
HHHCCCCCEEEEEEHHHCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEC
GGELFPQQLVKAKNWQAIAQLARNLTQELSIIT
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8145647 [H]