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The map label for this gene is glyA

Identifier: 75908297

GI number: 75908297

Start: 2566699

End: 2567982

Strand: Direct

Name: glyA

Synonym: Ava_2076

Alternate gene names: 75908297

Gene position: 2566699-2567982 (Clockwise)

Preceding gene: 75908294

Following gene: 75908298

Centisome position: 40.32

GC content: 47.9

Gene sequence:

>1284_bases
GTGACTAGAACAAATTCAGATTTTCTCACTAATTCTGATCCAGCGATCGCCGGATTGATTAATCAAGAACTCCAACGTCA
ACGGGACCACTTGGAGTTAATCGCCAGTGAAAACTTTACCTCGGCGGCTGTCTTGGCGGCTCAGGGTTCGGTACTGACAA
ATAAATACGCTGAGGGGCTGCCAGGTAAGCGCTACTACGGTGGTTGTGAGTTTATCGATAAAATCGAGCAAATAGCCATT
GACCGCGCTAAACAGCTATTTGGTGCCGCTCATGCCAACGTCCAACCCCATTCCGGCGCACAAGCCAATTTTGCTGTTTT
CCTGACTTTACTCGCACCAGGAGACAAAATCATGGGGATGGATTTGTCTCATGGTGGACACCTCACCCACGGTTCCCCGG
TAAACGTTTCCGGTAAGTGGTTCCAAGTTTGCCACTACGGTGTCAGTCAACAAACAGAACAACTAGACTATGACCAAATT
CGTGAGCTGGCGCTGAGGGAGCGTCCTAAGCTTTTGATTTGTGGTTATTCTGCTTATCCCCGAATTATTGACTTTGAAAA
GTTCCGCAGCATCGCCGATGAAGTTGGCGCGTATTTGCTGGCGGATATTGCCCACATTGCCGGTTTAGTCGCAAGTGGAC
TGCACCCCGACCCCATACCTTACTGTCACGTCGTCACAACTACCACCCATAAAACCCTCCGTGGCCCCAGAGGTGGTTTA
ATTTTGACCGGTGACGCAGAACTCGGTAAAAAATTGGATAAATCCGTTTTTCCTGGAAGCCAAGGGGGACCGTTAGAACA
CGTAATTGCTGGGAAAGCAGTTGCTTTTGGTGAAGCCTTAAAGCCAGAATTTCAAGGCTATTCAGCCCAAGTAATTGAGA
ATGCTCGTGCTTTGGCAAATCAACTCCAGAATCGTGGATTAAAGCTTGTATCCGATGGTACGGATAACCACCTAATGCTC
GTAGATTTACGGTCTGTGAACATGACAGGTAAACGAGCAGACCAGTTAGTGAGTGAAGTAAATATCACCGCTAATAAAAA
TACGGTTCCCTTCGATCCCCAATCACCATTTGTTACCAGTGGTTTGCGCTTGGGTTCCCCAGCTATGACTACACGAGGAA
TGGGAGAGGCAGAATTTACCGAAATTGGTAATATTATCGCCGATCGCCTACTCAACCCAGATTCAGACACAGTCGCCCAA
GATTGTAAACGCCGGGTAGCCGCATTGTGCGATCGCTTCCCCTTATATCCACACTTGGAAATTCCTGTACCAGTCTTAGC
TTAG

Upstream 100 bases:

>100_bases
GTAGCCAAAATAGATAGAAATCTTGATTGGTGGCTCTAAGTTATGTGCTGCGCTCAAACAAAATCAACTGTTGTACAAAT
TTCCATTAGGTATAGTTAAA

Downstream 100 bases:

>100_bases
GGATTGAAGAGGGATGAGGGAAAAATTCTTCTCTCATTCCTCACCCCTCAAGAGTTTTTGAATTTTGTTGGCGTAGCCTA
CTCTTCTCCTTCGGAGACGC

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTRTNSDFLTNSDPAIAGLINQELQRQRDHLELIASENFTSAAVLAAQGSVLTNKYAEGLPGKRYYGGCEFIDKIEQIAI
DRAKQLFGAAHANVQPHSGAQANFAVFLTLLAPGDKIMGMDLSHGGHLTHGSPVNVSGKWFQVCHYGVSQQTEQLDYDQI
RELALRERPKLLICGYSAYPRIIDFEKFRSIADEVGAYLLADIAHIAGLVASGLHPDPIPYCHVVTTTTHKTLRGPRGGL
ILTGDAELGKKLDKSVFPGSQGGPLEHVIAGKAVAFGEALKPEFQGYSAQVIENARALANQLQNRGLKLVSDGTDNHLML
VDLRSVNMTGKRADQLVSEVNITANKNTVPFDPQSPFVTSGLRLGSPAMTTRGMGEAEFTEIGNIIADRLLNPDSDTVAQ
DCKRRVAALCDRFPLYPHLEIPVPVLA

Sequences:

>Translated_427_residues
MTRTNSDFLTNSDPAIAGLINQELQRQRDHLELIASENFTSAAVLAAQGSVLTNKYAEGLPGKRYYGGCEFIDKIEQIAI
DRAKQLFGAAHANVQPHSGAQANFAVFLTLLAPGDKIMGMDLSHGGHLTHGSPVNVSGKWFQVCHYGVSQQTEQLDYDQI
RELALRERPKLLICGYSAYPRIIDFEKFRSIADEVGAYLLADIAHIAGLVASGLHPDPIPYCHVVTTTTHKTLRGPRGGL
ILTGDAELGKKLDKSVFPGSQGGPLEHVIAGKAVAFGEALKPEFQGYSAQVIENARALANQLQNRGLKLVSDGTDNHLML
VDLRSVNMTGKRADQLVSEVNITANKNTVPFDPQSPFVTSGLRLGSPAMTTRGMGEAEFTEIGNIIADRLLNPDSDTVAQ
DCKRRVAALCDRFPLYPHLEIPVPVLA
>Mature_426_residues
TRTNSDFLTNSDPAIAGLINQELQRQRDHLELIASENFTSAAVLAAQGSVLTNKYAEGLPGKRYYGGCEFIDKIEQIAID
RAKQLFGAAHANVQPHSGAQANFAVFLTLLAPGDKIMGMDLSHGGHLTHGSPVNVSGKWFQVCHYGVSQQTEQLDYDQIR
ELALRERPKLLICGYSAYPRIIDFEKFRSIADEVGAYLLADIAHIAGLVASGLHPDPIPYCHVVTTTTHKTLRGPRGGLI
LTGDAELGKKLDKSVFPGSQGGPLEHVIAGKAVAFGEALKPEFQGYSAQVIENARALANQLQNRGLKLVSDGTDNHLMLV
DLRSVNMTGKRADQLVSEVNITANKNTVPFDPQSPFVTSGLRLGSPAMTTRGMGEAEFTEIGNIIADRLLNPDSDTVAQD
CKRRVAALCDRFPLYPHLEIPVPVLA

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=406, Percent_Identity=47.5369458128079, Blast_Score=350, Evalue=2e-96,
Organism=Homo sapiens, GI261862350, Length=406, Percent_Identity=47.5369458128079, Blast_Score=350, Evalue=2e-96,
Organism=Homo sapiens, GI261862348, Length=406, Percent_Identity=47.5369458128079, Blast_Score=350, Evalue=2e-96,
Organism=Homo sapiens, GI19923315, Length=406, Percent_Identity=47.5369458128079, Blast_Score=350, Evalue=2e-96,
Organism=Homo sapiens, GI22547186, Length=401, Percent_Identity=48.1296758104738, Blast_Score=335, Evalue=4e-92,
Organism=Homo sapiens, GI261862346, Length=402, Percent_Identity=47.7611940298507, Blast_Score=334, Evalue=9e-92,
Organism=Homo sapiens, GI22547189, Length=387, Percent_Identity=46.2532299741602, Blast_Score=305, Evalue=4e-83,
Organism=Escherichia coli, GI1788902, Length=410, Percent_Identity=57.3170731707317, Blast_Score=472, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI25144732, Length=402, Percent_Identity=46.7661691542289, Blast_Score=358, Evalue=3e-99,
Organism=Caenorhabditis elegans, GI25144729, Length=402, Percent_Identity=46.7661691542289, Blast_Score=358, Evalue=4e-99,
Organism=Saccharomyces cerevisiae, GI6319739, Length=434, Percent_Identity=43.0875576036866, Blast_Score=358, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6323087, Length=401, Percent_Identity=44.139650872818, Blast_Score=345, Evalue=5e-96,
Organism=Drosophila melanogaster, GI24640005, Length=396, Percent_Identity=47.7272727272727, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI221329721, Length=396, Percent_Identity=47.7272727272727, Blast_Score=374, Evalue=1e-104,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_ANAVT (Q3MBD8)

Other databases:

- EMBL:   CP000117
- RefSeq:   YP_322593.1
- ProteinModelPortal:   Q3MBD8
- SMR:   Q3MBD8
- STRING:   Q3MBD8
- GeneID:   3680549
- GenomeReviews:   CP000117_GR
- KEGG:   ava:Ava_2076
- NMPDR:   fig|240292.3.peg.1481
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   YGGCANV
- PhylomeDB:   Q3MBD8
- ProtClustDB:   PRK00011
- BioCyc:   AVAR240292:AVA_2076-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 46272; Mature: 46141

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTNSDFLTNSDPAIAGLINQELQRQRDHLELIASENFTSAAVLAAQGSVLTNKYAEGL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHCCC
PGKRYYGGCEFIDKIEQIAIDRAKQLFGAAHANVQPHSGAQANFAVFLTLLAPGDKIMGM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCEEEE
DLSHGGHLTHGSPVNVSGKWFQVCHYGVSQQTEQLDYDQIRELALRERPKLLICGYSAYP
ECCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHCCCHHHHHHHHHHCCCCEEEECCCCCC
RIIDFEKFRSIADEVGAYLLADIAHIAGLVASGLHPDPIPYCHVVTTTTHKTLRGPRGGL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHCCCCCCE
ILTGDAELGKKLDKSVFPGSQGGPLEHVIAGKAVAFGEALKPEFQGYSAQVIENARALAN
EEECCHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHCHHHCCCHHHHHHHHHHHHH
QLQNRGLKLVSDGTDNHLMLVDLRSVNMTGKRADQLVSEVNITANKNTVPFDPQSPFVTS
HHHHCCEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHEEEECCCCCCCCCCCCHHHC
GLRLGSPAMTTRGMGEAEFTEIGNIIADRLLNPDSDTVAQDCKRRVAALCDRFPLYPHLE
CCCCCCCCHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
IPVPVLA
CCCCCCC
>Mature Secondary Structure 
TRTNSDFLTNSDPAIAGLINQELQRQRDHLELIASENFTSAAVLAAQGSVLTNKYAEGL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHCCC
PGKRYYGGCEFIDKIEQIAIDRAKQLFGAAHANVQPHSGAQANFAVFLTLLAPGDKIMGM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCEEEE
DLSHGGHLTHGSPVNVSGKWFQVCHYGVSQQTEQLDYDQIRELALRERPKLLICGYSAYP
ECCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHCCCHHHHHHHHHHCCCCEEEECCCCCC
RIIDFEKFRSIADEVGAYLLADIAHIAGLVASGLHPDPIPYCHVVTTTTHKTLRGPRGGL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHCCCCCCE
ILTGDAELGKKLDKSVFPGSQGGPLEHVIAGKAVAFGEALKPEFQGYSAQVIENARALAN
EEECCHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHCHHHCCCHHHHHHHHHHHHH
QLQNRGLKLVSDGTDNHLMLVDLRSVNMTGKRADQLVSEVNITANKNTVPFDPQSPFVTS
HHHHCCEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHEEEECCCCCCCCCCCCHHHC
GLRLGSPAMTTRGMGEAEFTEIGNIIADRLLNPDSDTVAQDCKRRVAALCDRFPLYPHLE
CCCCCCCCHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
IPVPVLA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA