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The map label for this gene is aat

Identifier: 75675949

GI number: 75675949

Start: 1926936

End: 1927613

Strand: Direct

Name: aat

Synonym: Nwi_1757

Alternate gene names: 75675949

Gene position: 1926936-1927613 (Clockwise)

Preceding gene: 75675948

Following gene: 75675951

Centisome position: 56.64

GC content: 65.93

Gene sequence:

>678_bases
ATGTCCCGCGAGACCGCCTCGTCCGAGATCACGCCCGAACTGCTGCTGCGCGCCTATGCCTGCGGCATTTTTCCGATGGC
GGAGAGTGTCGACGACCCGACGCTGTTCTGGGTCGAGCCGAAGATGCGCGGCGTGGTTCCACTCGAGAGCTTTCGAATTC
CGTCGCGGCTCGCGCGCACCGTTCGATCGGACAGTTTCACGGTGACCGTCGATACCGCATTCGACGCGGTGATCAACGGC
TGCGCCGCGCCCCAACCCGGCCGCAACAACACCTGGATCAACCATCGAATCCGTGAACTCTATATCGGCCTGCACGAACT
CGGCCACTGCCACAGTGTCGAGGTCTGGCGGGACAATGAACTGGCCGGCGGCCTTTACGGCGTCAGGCTCGGCCGGGCGT
TTTTCGGCGAGAGCATGTTCCATGTCGTGCGGGACGCGTCGAAGGTCGCGCTCGTGCATCTGGTCGCCCGGTTGATCGCC
GGAGGCTTCGTGCTGCTCGACACGCAGTTCGTCACCGACCATCTGCGGGGGTTCGGCGCCATCGAAATCCCGCGCCGGCG
CTACCGGGCGCTACTCGACGTCGCGCTGGAAGGCCGGGCCGATTTCGGAGCGCTGCCGCTCGACCGGCCGGTGCCGGGCG
CAGAAGCGCTCAAGGCCATCGCCGACCGAAGCTCTTAG

Upstream 100 bases:

>100_bases
AACCGACATCATCTCTCGGCACGCGATCTCCACTTGATCGCATCATTCGCTGGTAGATTATCGACGCGGAAACCTTCCAG
CTCTGCTATAAGATAACCGG

Downstream 100 bases:

>100_bases
AGCATGATCCCGACCCGAAGGGCCGCGCCAGCGCAAAGTAGGAACCGGCAGCCTTTTCTGATGGAATCAGAAACGAGGCT
TGCGGATTTTGATCCGGCGC

Product: leucyl/phenylalanyl-tRNA--protein transferase

Products: NA

Alternate protein names: L/F-transferase; Leucyltransferase; Phenyalanyltransferase

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MSRETASSEITPELLLRAYACGIFPMAESVDDPTLFWVEPKMRGVVPLESFRIPSRLARTVRSDSFTVTVDTAFDAVING
CAAPQPGRNNTWINHRIRELYIGLHELGHCHSVEVWRDNELAGGLYGVRLGRAFFGESMFHVVRDASKVALVHLVARLIA
GGFVLLDTQFVTDHLRGFGAIEIPRRRYRALLDVALEGRADFGALPLDRPVPGAEALKAIADRSS

Sequences:

>Translated_225_residues
MSRETASSEITPELLLRAYACGIFPMAESVDDPTLFWVEPKMRGVVPLESFRIPSRLARTVRSDSFTVTVDTAFDAVING
CAAPQPGRNNTWINHRIRELYIGLHELGHCHSVEVWRDNELAGGLYGVRLGRAFFGESMFHVVRDASKVALVHLVARLIA
GGFVLLDTQFVTDHLRGFGAIEIPRRRYRALLDVALEGRADFGALPLDRPVPGAEALKAIADRSS
>Mature_224_residues
SRETASSEITPELLLRAYACGIFPMAESVDDPTLFWVEPKMRGVVPLESFRIPSRLARTVRSDSFTVTVDTAFDAVINGC
AAPQPGRNNTWINHRIRELYIGLHELGHCHSVEVWRDNELAGGLYGVRLGRAFFGESMFHVVRDASKVALVHLVARLIAG
GFVLLDTQFVTDHLRGFGAIEIPRRRYRALLDVALEGRADFGALPLDRPVPGAEALKAIADRSS

Specific function: Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl-tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine

COG id: COG2360

COG function: function code O; Leu/Phe-tRNA-protein transferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L/F-transferase family

Homologues:

Organism=Escherichia coli, GI1787111, Length=191, Percent_Identity=43.979057591623, Blast_Score=146, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LFTR_NITWN (Q3SRS3)

Other databases:

- EMBL:   CP000115
- RefSeq:   YP_318370.1
- HSSP:   P0A8P1
- ProteinModelPortal:   Q3SRS3
- SMR:   Q3SRS3
- STRING:   Q3SRS3
- GeneID:   3676480
- GenomeReviews:   CP000115_GR
- KEGG:   nwi:Nwi_1757
- NMPDR:   fig|323098.3.peg.1549
- eggNOG:   COG2360
- HOGENOM:   HBG485363
- OMA:   GEPILWW
- PhylomeDB:   Q3SRS3
- ProtClustDB:   PRK00301
- BioCyc:   NWIN323098:NWI_1757-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00688
- InterPro:   IPR016181
- InterPro:   IPR004616
- TIGRFAMs:   TIGR00667

Pfam domain/function: PF03588 Leu_Phe_trans; SSF55729 Acyl_CoA_acyltransferase

EC number: =2.3.2.6

Molecular weight: Translated: 24882; Mature: 24751

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRETASSEITPELLLRAYACGIFPMAESVDDPTLFWVEPKMRGVVPLESFRIPSRLART
CCCCCCCCCCCHHHHHHHHHHCCCHHHCCCCCCEEEEECCCCCCCCCHHHHCCHHHHHHH
VRSDSFTVTVDTAFDAVINGCAAPQPGRNNTWINHRIRELYIGLHELGHCHSVEVWRDNE
HCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCC
LAGGLYGVRLGRAFFGESMFHVVRDASKVALVHLVARLIAGGFVLLDTQFVTDHLRGFGA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHCCCCC
IEIPRRRYRALLDVALEGRADFGALPLDRPVPGAEALKAIADRSS
EECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
SRETASSEITPELLLRAYACGIFPMAESVDDPTLFWVEPKMRGVVPLESFRIPSRLART
CCCCCCCCCCHHHHHHHHHHCCCHHHCCCCCCEEEEECCCCCCCCCHHHHCCHHHHHHH
VRSDSFTVTVDTAFDAVINGCAAPQPGRNNTWINHRIRELYIGLHELGHCHSVEVWRDNE
HCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCC
LAGGLYGVRLGRAFFGESMFHVVRDASKVALVHLVARLIAGGFVLLDTQFVTDHLRGFGA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHCCCCC
IEIPRRRYRALLDVALEGRADFGALPLDRPVPGAEALKAIADRSS
EECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA