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The map label for this gene is tyrS

Identifier: 75675860

GI number: 75675860

Start: 1815311

End: 1816564

Strand: Direct

Name: tyrS

Synonym: Nwi_1668

Alternate gene names: 75675860

Gene position: 1815311-1816564 (Clockwise)

Preceding gene: 75675858

Following gene: 75675862

Centisome position: 53.36

GC content: 60.77

Gene sequence:

>1254_bases
ATGGTCGCTTTCAAGTCCGATTTTCTCAACACGCTTCAGGAACGGGGCTTCATCCACCAGTGCTCCGATTTCGAAGGCCT
TGATGCGCTGGCGTCCAGAAATCAGGTGACGGCCTACACCGGCTATGACTGCACCGCGCCGTCGCTGCATGTCGGTCACC
TGCTCTCGATCATGATGATGCACTGGCTGCAACAGACTGGAAACAAGCCGATCGCCCTGATGGGTGGCGGCACCACGCGC
GTGGGCGATCCGTCGGGCCGCGACGAAACGCGCAAAATTCTGAGCTATGACCAGATCGACGCCAACAAGGAATCGATCAA
GGGCACATTCTTGAAGTTCCTGGTTTTCGGCGACGGCAAGAACGACGCCATCATGACGGACAATGCCGAGTGGCTCACCA
GGCTCAACTATATCGAGATGCTGCGCGACGTCGGCCGGCATTTTTCCATCAACCGCATGCTCACGATGGACAGCGTGAAG
ATGCGTCTCGAGCGCGAGCAGGAGTTGTCGTTCATCGAGTTCAACTACATGATCCTTCAGTCCTACGATTATGTGGAACT
GGCGCGCCGCCACGGCTGCAACCTGCAAATGGGCGGTTCGGATCAGTGGGGCAACATCGTCAACGGCGTCGATCTCGGCC
GCCGCATGGGCACGCATCAGCTCTACGCGCTGACCTGCCCGCTGCTCACGACATCGTCGGGCGCCAAGATGGGCAAGACC
GCGGCGGGCGCGGTCTGGCTCAACGCCACCATGCTGCCGGTCTACGATTACTGGCAGTACTGGCGCAACGTCGAGGACGC
CGACGTCGGCAGATTCCTGAAACTGTTCACGATCCTGCCGATGGGTGAGATCGCGAAGCTGGCTGCGTTGCAGGGCTCAG
AGATCAACGAGGCCAAGAAGGTGCTCGCGACCGAGGCGACCGCGCTTCTGCATGGTCGCGACGAAGCCGAGAAAGCCGCC
GACACCGCGCGCACGACGTTCGAGCAAGGCGCTATTTCCGAGAGTCTTCCCACACTGGATATTCCGCGGAGCGAGCTTGC
AAGCGGCGCCGGCGTGCTCGCACTGTTTGTGAAGGCGGGCCTTGTCGCCTCGAACGGAGAAGCGCGCCGCCAGATCAAGG
GTGGTGGACTTCGCGTCAACGATGTCGCCGTCGCCGACGACAAGATGGTGCTGACGCCGGATCACCTCACGCCCGAAGGC
GTGATCAAACTTTCAATGGGCAAGAAACGCCACGTCCTGCTGCGGCCCGCATAG

Upstream 100 bases:

>100_bases
CACACACTTGACACGCAGTATAACCGGGCGGCGGCTGACAGCTATCCTGTGCCGAATGGTCTGCGCGCTCAGTTGAACAA
GAGATTAGAGTTATAGTGCC

Downstream 100 bases:

>100_bases
CGCGTCCGGAGAACGCGATCGCGTTCAGTTCCCGCTCGTTGCCGGAAACTCGATCTGGTTGGCTGTTCTTCCCGTCTGGA
ACTCAAAAATCTTGCGCACC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MVAFKSDFLNTLQERGFIHQCSDFEGLDALASRNQVTAYTGYDCTAPSLHVGHLLSIMMMHWLQQTGNKPIALMGGGTTR
VGDPSGRDETRKILSYDQIDANKESIKGTFLKFLVFGDGKNDAIMTDNAEWLTRLNYIEMLRDVGRHFSINRMLTMDSVK
MRLEREQELSFIEFNYMILQSYDYVELARRHGCNLQMGGSDQWGNIVNGVDLGRRMGTHQLYALTCPLLTTSSGAKMGKT
AAGAVWLNATMLPVYDYWQYWRNVEDADVGRFLKLFTILPMGEIAKLAALQGSEINEAKKVLATEATALLHGRDEAEKAA
DTARTTFEQGAISESLPTLDIPRSELASGAGVLALFVKAGLVASNGEARRQIKGGGLRVNDVAVADDKMVLTPDHLTPEG
VIKLSMGKKRHVLLRPA

Sequences:

>Translated_417_residues
MVAFKSDFLNTLQERGFIHQCSDFEGLDALASRNQVTAYTGYDCTAPSLHVGHLLSIMMMHWLQQTGNKPIALMGGGTTR
VGDPSGRDETRKILSYDQIDANKESIKGTFLKFLVFGDGKNDAIMTDNAEWLTRLNYIEMLRDVGRHFSINRMLTMDSVK
MRLEREQELSFIEFNYMILQSYDYVELARRHGCNLQMGGSDQWGNIVNGVDLGRRMGTHQLYALTCPLLTTSSGAKMGKT
AAGAVWLNATMLPVYDYWQYWRNVEDADVGRFLKLFTILPMGEIAKLAALQGSEINEAKKVLATEATALLHGRDEAEKAA
DTARTTFEQGAISESLPTLDIPRSELASGAGVLALFVKAGLVASNGEARRQIKGGGLRVNDVAVADDKMVLTPDHLTPEG
VIKLSMGKKRHVLLRPA
>Mature_417_residues
MVAFKSDFLNTLQERGFIHQCSDFEGLDALASRNQVTAYTGYDCTAPSLHVGHLLSIMMMHWLQQTGNKPIALMGGGTTR
VGDPSGRDETRKILSYDQIDANKESIKGTFLKFLVFGDGKNDAIMTDNAEWLTRLNYIEMLRDVGRHFSINRMLTMDSVK
MRLEREQELSFIEFNYMILQSYDYVELARRHGCNLQMGGSDQWGNIVNGVDLGRRMGTHQLYALTCPLLTTSSGAKMGKT
AAGAVWLNATMLPVYDYWQYWRNVEDADVGRFLKLFTILPMGEIAKLAALQGSEINEAKKVLATEATALLHGRDEAEKAA
DTARTTFEQGAISESLPTLDIPRSELASGAGVLALFVKAGLVASNGEARRQIKGGGLRVNDVAVADDKMVLTPDHLTPEG
VIKLSMGKKRHVLLRPA

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=399, Percent_Identity=34.3358395989975, Blast_Score=219, Evalue=6e-57,
Organism=Escherichia coli, GI1787925, Length=422, Percent_Identity=37.6777251184834, Blast_Score=294, Evalue=6e-81,
Organism=Caenorhabditis elegans, GI25145802, Length=360, Percent_Identity=30.8333333333333, Blast_Score=174, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI25145805, Length=274, Percent_Identity=28.8321167883212, Blast_Score=122, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6325160, Length=329, Percent_Identity=34.0425531914894, Blast_Score=202, Evalue=6e-53,
Organism=Drosophila melanogaster, GI20130383, Length=436, Percent_Identity=34.4036697247706, Blast_Score=237, Evalue=9e-63,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_NITWN (Q3SS12)

Other databases:

- EMBL:   CP000115
- RefSeq:   YP_318281.1
- ProteinModelPortal:   Q3SS12
- SMR:   Q3SS12
- STRING:   Q3SS12
- GeneID:   3674700
- GenomeReviews:   CP000115_GR
- KEGG:   nwi:Nwi_1668
- NMPDR:   fig|323098.3.peg.1266
- eggNOG:   COG0162
- HOGENOM:   HBG288125
- OMA:   TFYIGFD
- PhylomeDB:   Q3SS12
- ProtClustDB:   PRK05912
- BioCyc:   NWIN323098:NWI_1668-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 46100; Mature: 46100

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 39-39 BINDING 176-176 BINDING 180-180 BINDING 239-239

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAFKSDFLNTLQERGFIHQCSDFEGLDALASRNQVTAYTGYDCTAPSLHVGHLLSIMMM
CCCCHHHHHHHHHHCCCEECCCCCCCHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHH
HWLQQTGNKPIALMGGGTTRVGDPSGRDETRKILSYDQIDANKESIKGTFLKFLVFGDGK
HHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHEEEEEEEEECCC
NDAIMTDNAEWLTRLNYIEMLRDVGRHFSINRMLTMDSVKMRLEREQELSFIEFNYMILQ
CCEEEECCHHHHHHHHHHHHHHHHHCCEECCEEEEHHHHHHHHHHHHCCCEEEEEEEEEE
SYDYVELARRHGCNLQMGGSDQWGNIVNGVDLGRRMGTHQLYALTCPLLTTSSGAKMGKT
CCCHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECEEECCCCCCCCCC
AAGAVWLNATMLPVYDYWQYWRNVEDADVGRFLKLFTILPMGEIAKLAALQGSEINEAKK
CCCEEEEEEEECHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHH
VLATEATALLHGRDEAEKAADTARTTFEQGAISESLPTLDIPRSELASGAGVLALFVKAG
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHCCCHHHHHHHHHC
LVASNGEARRQIKGGGLRVNDVAVADDKMVLTPDHLTPEGVIKLSMGKKRHVLLRPA
CEECCCHHHHHHCCCCEEEEEEEEECCCEEECCCCCCCCCEEEEECCCCEEEEEECC
>Mature Secondary Structure
MVAFKSDFLNTLQERGFIHQCSDFEGLDALASRNQVTAYTGYDCTAPSLHVGHLLSIMMM
CCCCHHHHHHHHHHCCCEECCCCCCCHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHH
HWLQQTGNKPIALMGGGTTRVGDPSGRDETRKILSYDQIDANKESIKGTFLKFLVFGDGK
HHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHEEEEEEEEECCC
NDAIMTDNAEWLTRLNYIEMLRDVGRHFSINRMLTMDSVKMRLEREQELSFIEFNYMILQ
CCEEEECCHHHHHHHHHHHHHHHHHCCEECCEEEEHHHHHHHHHHHHCCCEEEEEEEEEE
SYDYVELARRHGCNLQMGGSDQWGNIVNGVDLGRRMGTHQLYALTCPLLTTSSGAKMGKT
CCCHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECEEECCCCCCCCCC
AAGAVWLNATMLPVYDYWQYWRNVEDADVGRFLKLFTILPMGEIAKLAALQGSEINEAKK
CCCEEEEEEEECHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHH
VLATEATALLHGRDEAEKAADTARTTFEQGAISESLPTLDIPRSELASGAGVLALFVKAG
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHCCCHHHHHHHHHC
LVASNGEARRQIKGGGLRVNDVAVADDKMVLTPDHLTPEGVIKLSMGKKRHVLLRPA
CEECCCHHHHHHCCCCEEEEEEEEECCCEEECCCCCCCCCEEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA