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The map label for this gene is folE [H]

Identifier: 75675521

GI number: 75675521

Start: 1458492

End: 1459217

Strand: Direct

Name: folE [H]

Synonym: Nwi_1329

Alternate gene names: 75675521

Gene position: 1458492-1459217 (Clockwise)

Preceding gene: 75675519

Following gene: 75675522

Centisome position: 42.87

GC content: 61.71

Gene sequence:

>726_bases
ATGGACGCTTTGATCAAGCCGCTGCGCAGCGGCAAGCCTTCTGACATCAACAAGGCTTCTGATATCAAATCCAGTAACGC
CAAGCCTGCCGGCTTTTCTCCCGCTCAACTGGACCCGTCGGAGTTTATGGCGGCGGTTCAGCCGAACCGGCAGCGCCCGT
CGAGGCATGAAGCTGAAGACGCGGTCAGAACGTTGCTGGCCTATATCGGCGAAAATCCCGACCGGGAGGGGCTGCTCGAT
ACCCCGCGCCGGGTGATCGAATCGTATGACGAGATTTATCAAGGCTATCATCAGTGTCCGGCCGAAGTGTTGAATCGGAC
CTTCGGTGAAACCGCCGGCTATGATGATTTCGTGCTGGTGCGCGACATCCAGTTCACTTCGCAGTGCGAGCATCACATGA
TGCCGTTCTATGGCAAAGCGCATATCGCCTATACGCCGGTGGAGCGGGTGGTCGGGCTGTCCAAGCTGGCGCGTCTGACC
GACATCTTCGCCCGGAGACTCCAGACCCAGGAGCACATGACCGCGCAGATCGCCGCGGCCGTGGACGAGGTGCTCAAGCC
GCGCGGCGTCGCCGTGATGATCGAGGCCGAGCATACCTGCATGTCGGTGCGCGGCGTCGCCAAGCATGGCGCGATGACCG
TCACCAGCCGGTTCACCGGCGTGTTCCGAGACGATCCGGCGGAGCAGGCGCGTTTCCTGTCCATGGTACGGGGGCAGCCG
CGTTAG

Upstream 100 bases:

>100_bases
TTAAATATCAAGGGACTCAGGCGTCCGGTGGCGAGAAAGGCCGCCCCGTCTGCCCGGTGACACCTCCGGTCCAACGTCAG
GCCGGTCGAACGGAGTGAAG

Downstream 100 bases:

>100_bases
TCCCCATCCGCGTTTCGGAGCGAAGCCATTGCTATTCCGGGTTCGCGTGAAGAAAGCACGTCAAGCATAAAGAAAGATAG
AGTCTTTCACCTTTTCCATG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MDALIKPLRSGKPSDINKASDIKSSNAKPAGFSPAQLDPSEFMAAVQPNRQRPSRHEAEDAVRTLLAYIGENPDREGLLD
TPRRVIESYDEIYQGYHQCPAEVLNRTFGETAGYDDFVLVRDIQFTSQCEHHMMPFYGKAHIAYTPVERVVGLSKLARLT
DIFARRLQTQEHMTAQIAAAVDEVLKPRGVAVMIEAEHTCMSVRGVAKHGAMTVTSRFTGVFRDDPAEQARFLSMVRGQP
R

Sequences:

>Translated_241_residues
MDALIKPLRSGKPSDINKASDIKSSNAKPAGFSPAQLDPSEFMAAVQPNRQRPSRHEAEDAVRTLLAYIGENPDREGLLD
TPRRVIESYDEIYQGYHQCPAEVLNRTFGETAGYDDFVLVRDIQFTSQCEHHMMPFYGKAHIAYTPVERVVGLSKLARLT
DIFARRLQTQEHMTAQIAAAVDEVLKPRGVAVMIEAEHTCMSVRGVAKHGAMTVTSRFTGVFRDDPAEQARFLSMVRGQP
R
>Mature_241_residues
MDALIKPLRSGKPSDINKASDIKSSNAKPAGFSPAQLDPSEFMAAVQPNRQRPSRHEAEDAVRTLLAYIGENPDREGLLD
TPRRVIESYDEIYQGYHQCPAEVLNRTFGETAGYDDFVLVRDIQFTSQCEHHMMPFYGKAHIAYTPVERVVGLSKLARLT
DIFARRLQTQEHMTAQIAAAVDEVLKPRGVAVMIEAEHTCMSVRGVAKHGAMTVTSRFTGVFRDDPAEQARFLSMVRGQP
R

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=223, Percent_Identity=42.6008968609865, Blast_Score=182, Evalue=2e-46,
Organism=Homo sapiens, GI4503949, Length=223, Percent_Identity=42.6008968609865, Blast_Score=182, Evalue=2e-46,
Organism=Homo sapiens, GI66932970, Length=193, Percent_Identity=39.3782383419689, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI66932972, Length=182, Percent_Identity=41.2087912087912, Blast_Score=139, Evalue=2e-33,
Organism=Escherichia coli, GI1788476, Length=198, Percent_Identity=34.8484848484849, Blast_Score=110, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17560486, Length=178, Percent_Identity=46.6292134831461, Blast_Score=174, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6321706, Length=178, Percent_Identity=47.1910112359551, Blast_Score=168, Evalue=6e-43,
Organism=Drosophila melanogaster, GI24656782, Length=178, Percent_Identity=49.438202247191, Blast_Score=171, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24656791, Length=178, Percent_Identity=49.438202247191, Blast_Score=171, Evalue=5e-43,
Organism=Drosophila melanogaster, GI24656796, Length=178, Percent_Identity=49.438202247191, Blast_Score=171, Evalue=6e-43,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 26917; Mature: 26917

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDALIKPLRSGKPSDINKASDIKSSNAKPAGFSPAQLDPSEFMAAVQPNRQRPSRHEAED
CCHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHH
AVRTLLAYIGENPDREGLLDTPRRVIESYDEIYQGYHQCPAEVLNRTFGETAGYDDFVLV
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCEEEE
RDIQFTSQCEHHMMPFYGKAHIAYTPVERVVGLSKLARLTDIFARRLQTQEHMTAQIAAA
ECCHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDEVLKPRGVAVMIEAEHTCMSVRGVAKHGAMTVTSRFTGVFRDDPAEQARFLSMVRGQP
HHHHHCCCCCEEEEECCHHHHHHHHHHHCCCEEHHHHHHHCCCCCHHHHHHHHHHHCCCC
R
C
>Mature Secondary Structure
MDALIKPLRSGKPSDINKASDIKSSNAKPAGFSPAQLDPSEFMAAVQPNRQRPSRHEAED
CCHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHH
AVRTLLAYIGENPDREGLLDTPRRVIESYDEIYQGYHQCPAEVLNRTFGETAGYDDFVLV
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCEEEE
RDIQFTSQCEHHMMPFYGKAHIAYTPVERVVGLSKLARLTDIFARRLQTQEHMTAQIAAA
ECCHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDEVLKPRGVAVMIEAEHTCMSVRGVAKHGAMTVTSRFTGVFRDDPAEQARFLSMVRGQP
HHHHHCCCCCEEEEECCHHHHHHHHHHHCCCEEHHHHHHHCCCCCHHHHHHHHHHHCCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA