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The map label for this gene is hisI

Identifier: 75675522

GI number: 75675522

Start: 1459462

End: 1459899

Strand: Direct

Name: hisI

Synonym: Nwi_1330

Alternate gene names: 75675522

Gene position: 1459462-1459899 (Clockwise)

Preceding gene: 75675521

Following gene: 75675523

Centisome position: 42.9

GC content: 63.47

Gene sequence:

>438_bases
GTGTCTGGATCGAAATCTTCATCTGAGACCGAGGAAGGGCTGGCGTTCCAGCCCTCCTTTGACGCGTCCGGGCTCGTGAC
CTGCGTAGCCACGGATGCCAAAACCGGCGACGTGCTGATGGTAGCGCACATGAATGAAGAGGCGCTGCGGCGGACCGTCG
AGACCGGCGACGCCTGGTATTACAGCCGATCGCGCAAGGCGTTATGGCGGAAGGGCGAGAGTTCGGGTCAGGTCCAGCGC
GTGCTGGAGATGCGGACGGACTGCGATCAGGACGCTGTCTGGATCAAGGTCGAGCAGCAGGGCGCCGCCTGCCATACCGG
GCGGCGCTCGTGCTTCTACCGGGCGGTGGCAAAAGGCGAGGGCGCGGGAATCCGTCTTGCCTTTGTGGATGCTGAAAGGC
TCTTCGATCCGGCCGAGGTTTATCGCGCGAAAGCCTGA

Upstream 100 bases:

>100_bases
AACCATGCTCTCGAAGTGGTTGTCTGGTCGCATTTTCTTGCGGCGACCGGTATCCACTTCGCCGGAAAATGCTGTAGGTC
CTATTCCGTAGGGATTTCCT

Downstream 100 bases:

>100_bases
CGGGTTTTCGGGCTTTGCTCGCGACCTGCGTTAAGGCGCCGTTAACCATAATCTTCCACCTTGCGCTTCTGGAGCGTTTC
AAGCGAAGTGGACACCGGTT

Product: phosphoribosyl-AMP cyclohydrolase

Products: NA

Alternate protein names: PRA-CH

Number of amino acids: Translated: 145; Mature: 144

Protein sequence:

>145_residues
MSGSKSSSETEEGLAFQPSFDASGLVTCVATDAKTGDVLMVAHMNEEALRRTVETGDAWYYSRSRKALWRKGESSGQVQR
VLEMRTDCDQDAVWIKVEQQGAACHTGRRSCFYRAVAKGEGAGIRLAFVDAERLFDPAEVYRAKA

Sequences:

>Translated_145_residues
MSGSKSSSETEEGLAFQPSFDASGLVTCVATDAKTGDVLMVAHMNEEALRRTVETGDAWYYSRSRKALWRKGESSGQVQR
VLEMRTDCDQDAVWIKVEQQGAACHTGRRSCFYRAVAKGEGAGIRLAFVDAERLFDPAEVYRAKA
>Mature_144_residues
SGSKSSSETEEGLAFQPSFDASGLVTCVATDAKTGDVLMVAHMNEEALRRTVETGDAWYYSRSRKALWRKGESSGQVQRV
LEMRTDCDQDAVWIKVEQQGAACHTGRRSCFYRAVAKGEGAGIRLAFVDAERLFDPAEVYRAKA

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PRA-CH family

Homologues:

Organism=Escherichia coli, GI2367128, Length=127, Percent_Identity=36.2204724409449, Blast_Score=90, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6319819, Length=90, Percent_Identity=37.7777777777778, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS3_NITWN (Q3ST00)

Other databases:

- EMBL:   CP000115
- RefSeq:   YP_317943.1
- ProteinModelPortal:   Q3ST00
- SMR:   Q3ST00
- STRING:   Q3ST00
- GeneID:   3676596
- GenomeReviews:   CP000115_GR
- KEGG:   nwi:Nwi_1330
- NMPDR:   fig|323098.3.peg.2199
- eggNOG:   COG0139
- HOGENOM:   HBG294308
- OMA:   LWLKGES
- PhylomeDB:   Q3ST00
- ProtClustDB:   PRK00051
- BioCyc:   NWIN323098:NWI_1330-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01021
- InterPro:   IPR002496
- ProDom:   PD002610

Pfam domain/function: PF01502 PRA-CH

EC number: =3.5.4.19

Molecular weight: Translated: 15977; Mature: 15846

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGSKSSSETEEGLAFQPSFDASGLVTCVATDAKTGDVLMVAHMNEEALRRTVETGDAWY
CCCCCCCCCCCCCEEECCCCCCCCEEEEEECCCCCCCEEEEEECCHHHHHHHHHCCCEEE
YSRSRKALWRKGESSGQVQRVLEMRTDCDQDAVWIKVEQQGAACHTGRRSCFYRAVAKGE
EECCHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCC
GAGIRLAFVDAERLFDPAEVYRAKA
CCCEEEEEECHHHCCCHHHHHCCCC
>Mature Secondary Structure 
SGSKSSSETEEGLAFQPSFDASGLVTCVATDAKTGDVLMVAHMNEEALRRTVETGDAWY
CCCCCCCCCCCCEEECCCCCCCCEEEEEECCCCCCCEEEEEECCHHHHHHHHHCCCEEE
YSRSRKALWRKGESSGQVQRVLEMRTDCDQDAVWIKVEQQGAACHTGRRSCFYRAVAKGE
EECCHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCC
GAGIRLAFVDAERLFDPAEVYRAKA
CCCEEEEEECHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA