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The map label for this gene is mdh1 [H]

Identifier: 75674617

GI number: 75674617

Start: 469971

End: 470939

Strand: Direct

Name: mdh1 [H]

Synonym: Nwi_0419

Alternate gene names: 75674617

Gene position: 469971-470939 (Clockwise)

Preceding gene: 75674616

Following gene: 75674618

Centisome position: 13.81

GC content: 62.75

Gene sequence:

>969_bases
ATGGCGCGCGACAAGATTGGCTTGATCGGCTCCGGCCAGATCGGAGGGACGCTCGCTCATCTTATCGGACTGAAGGAACT
CGGCGACGTCGTGATGTTCGATATCGCGGATGGCGTTCCGCAAGGTAAATCGCTCGATATTGCCCAGTCGTCCCCGGTAG
AAGGGTTCGACGCCAGACTGGCCGGCACCAATTCCTATGAAGCGCTCGAGGGCGCCGGCGTCTGCATCGTCACCGCCGGT
GTTCCGCGCAAGCCGGGCATGAGCCGCGACGATCTGCTCGGCATCAATCTCAAGGTGATGGAACAGGTCGGCGCAGGCAT
CAGGAAATATGCGCCGGATGCGTTCGTCATCTGCATCACCAATCCGCTCGACGCCATGGTGTGGGCGCTCCAGAAGGCCT
CCGGTCTGCCGGCGAAGAAGGTGGTCGGCATGGCCGGCGTGCTTGATTCCTCGCGCTTCCGCTATTTTCTCGCGGACGAG
TTCAACGTCTCGGTGGAAGACGTGACGGCCTTCGTGCTTGGCGGTCATGGCGACAGCATGGTGCCGCTGGTCAAATACTC
GACGGTGGCGGGCATTCCGCTGCCGGACCTCGTCAAGATGGGCTGGACCTCGCAGGCGCGCATCGATGAAATCGTGGATC
GCACCCGCAACGGCGGCGCCGAGATCGTCAATCTGCTCAAGACCGGCTCGGCCTTCTATGCCCCCGCCGCCTCCGCGATC
GCGATGGCGGAAAGTTTCCTGCGCGACAAGAAGCGCGTGCTGCCTTGCGCCGCCTATCTCAACGGCGAGTTCGGGGTATA
CGACATGTATGTCGGCGTGCCCGTGGTGATCGGCGCCAAGGGCGTCGAACGCATTGTCGAGATCGAACTGGCGGGCAGGG
ATCGGGAAGCCTTCGACAGATCGGTCGCCGCGGTTCAGGGGCTCGTCGATGCCTGCAAGAAGATCGCCCCTGATCTGCTC
GACCGTTGA

Upstream 100 bases:

>100_bases
CGCGTTTTCTTCACGCGAACCCATCACTCGCTCGAAAACAACCACGATCCGTCGAGGTTTTTCCTTCCCGTCTCTTATTG
TCATCAAAGGATGAATTCCC

Downstream 100 bases:

>100_bases
TCGGCATTGCCTCGGTTCGGTGCCAAAAGATGCGCGTGCGGTCTTGGAGGCATGCTTGGAGGCATATCGTGGGCGGGGCT
ATCCTGGCCGACGGAAACGA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MARDKIGLIGSGQIGGTLAHLIGLKELGDVVMFDIADGVPQGKSLDIAQSSPVEGFDARLAGTNSYEALEGAGVCIVTAG
VPRKPGMSRDDLLGINLKVMEQVGAGIRKYAPDAFVICITNPLDAMVWALQKASGLPAKKVVGMAGVLDSSRFRYFLADE
FNVSVEDVTAFVLGGHGDSMVPLVKYSTVAGIPLPDLVKMGWTSQARIDEIVDRTRNGGAEIVNLLKTGSAFYAPAASAI
AMAESFLRDKKRVLPCAAYLNGEFGVYDMYVGVPVVIGAKGVERIVEIELAGRDREAFDRSVAAVQGLVDACKKIAPDLL
DR

Sequences:

>Translated_322_residues
MARDKIGLIGSGQIGGTLAHLIGLKELGDVVMFDIADGVPQGKSLDIAQSSPVEGFDARLAGTNSYEALEGAGVCIVTAG
VPRKPGMSRDDLLGINLKVMEQVGAGIRKYAPDAFVICITNPLDAMVWALQKASGLPAKKVVGMAGVLDSSRFRYFLADE
FNVSVEDVTAFVLGGHGDSMVPLVKYSTVAGIPLPDLVKMGWTSQARIDEIVDRTRNGGAEIVNLLKTGSAFYAPAASAI
AMAESFLRDKKRVLPCAAYLNGEFGVYDMYVGVPVVIGAKGVERIVEIELAGRDREAFDRSVAAVQGLVDACKKIAPDLL
DR
>Mature_321_residues
ARDKIGLIGSGQIGGTLAHLIGLKELGDVVMFDIADGVPQGKSLDIAQSSPVEGFDARLAGTNSYEALEGAGVCIVTAGV
PRKPGMSRDDLLGINLKVMEQVGAGIRKYAPDAFVICITNPLDAMVWALQKASGLPAKKVVGMAGVLDSSRFRYFLADEF
NVSVEDVTAFVLGGHGDSMVPLVKYSTVAGIPLPDLVKMGWTSQARIDEIVDRTRNGGAEIVNLLKTGSAFYAPAASAIA
MAESFLRDKKRVLPCAAYLNGEFGVYDMYVGVPVVIGAKGVERIVEIELAGRDREAFDRSVAAVQGLVDACKKIAPDLLD
R

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=305, Percent_Identity=28.1967213114754, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI221136809, Length=305, Percent_Identity=28.1967213114754, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI9257228, Length=308, Percent_Identity=28.8961038961039, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI4504973, Length=308, Percent_Identity=28.8961038961039, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI15082234, Length=318, Percent_Identity=24.5283018867925, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI5031857, Length=312, Percent_Identity=27.2435897435897, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI260099723, Length=312, Percent_Identity=27.2435897435897, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI291575128, Length=323, Percent_Identity=27.8637770897833, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI4557032, Length=323, Percent_Identity=27.8637770897833, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI260099727, Length=200, Percent_Identity=30.5, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI260099725, Length=200, Percent_Identity=30.5, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI207028494, Length=195, Percent_Identity=27.6923076923077, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI21735621, Length=292, Percent_Identity=26.3698630136986, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI103472011, Length=319, Percent_Identity=20.3761755485893, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1789632, Length=325, Percent_Identity=29.5384615384615, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17535107, Length=245, Percent_Identity=27.7551020408163, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17554310, Length=312, Percent_Identity=29.1666666666667, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6322765, Length=300, Percent_Identity=29.3333333333333, Blast_Score=86, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6320125, Length=243, Percent_Identity=28.3950617283951, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324446, Length=255, Percent_Identity=27.0588235294118, Blast_Score=69, Evalue=7e-13,
Organism=Drosophila melanogaster, GI17136226, Length=301, Percent_Identity=27.906976744186, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI45550422, Length=300, Percent_Identity=26, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24647881, Length=287, Percent_Identity=28.5714285714286, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24663595, Length=266, Percent_Identity=28.9473684210526, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24663599, Length=308, Percent_Identity=25.974025974026, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 34062; Mature: 33931

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDKIGLIGSGQIGGTLAHLIGLKELGDVVMFDIADGVPQGKSLDIAQSSPVEGFDARL
CCCCCCCEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEE
AGTNSYEALEGAGVCIVTAGVPRKPGMSRDDLLGINLKVMEQVGAGIRKYAPDAFVICIT
CCCCCCHHHCCCCEEEEECCCCCCCCCCCCCEECCHHHHHHHHCCHHHHHCCCEEEEEEC
NPLDAMVWALQKASGLPAKKVVGMAGVLDSSRFRYFLADEFNVSVEDVTAFVLGGHGDSM
CHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHEEEEEEECCCCCC
VPLVKYSTVAGIPLPDLVKMGWTSQARIDEIVDRTRNGGAEIVNLLKTGSAFYAPAASAI
CEEEEEEEECCCCCHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCHHCHHHHHH
AMAESFLRDKKRVLPCAAYLNGEFGVYDMYVGVPVVIGAKGVERIVEIELAGRDREAFDR
HHHHHHHHHHHHHCCHHHHCCCCCCEEEEEECCEEEECCHHHCEEEEEEECCCCHHHHHH
SVAAVQGLVDACKKIAPDLLDR
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARDKIGLIGSGQIGGTLAHLIGLKELGDVVMFDIADGVPQGKSLDIAQSSPVEGFDARL
CCCCCCEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEE
AGTNSYEALEGAGVCIVTAGVPRKPGMSRDDLLGINLKVMEQVGAGIRKYAPDAFVICIT
CCCCCCHHHCCCCEEEEECCCCCCCCCCCCCEECCHHHHHHHHCCHHHHHCCCEEEEEEC
NPLDAMVWALQKASGLPAKKVVGMAGVLDSSRFRYFLADEFNVSVEDVTAFVLGGHGDSM
CHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHEEEEEEECCCCCC
VPLVKYSTVAGIPLPDLVKMGWTSQARIDEIVDRTRNGGAEIVNLLKTGSAFYAPAASAI
CEEEEEEEECCCCCHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCHHCHHHHHH
AMAESFLRDKKRVLPCAAYLNGEFGVYDMYVGVPVVIGAKGVERIVEIELAGRDREAFDR
HHHHHHHHHHHHHCCHHHHCCCCCCEEEEEECCEEEECCHHHCEEEEEEECCCCHHHHHH
SVAAVQGLVDACKKIAPDLLDR
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA