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The map label for this gene is tesA [H]

Identifier: 75674614

GI number: 75674614

Start: 467056

End: 467811

Strand: Direct

Name: tesA [H]

Synonym: Nwi_0416

Alternate gene names: 75674614

Gene position: 467056-467811 (Clockwise)

Preceding gene: 75674610

Following gene: 75674615

Centisome position: 13.73

GC content: 61.11

Gene sequence:

>756_bases
ATGTGGTCCAGGTTGCTTCCGTATGGAAGACCTCCGGAGCACCTGAAGCACAATGACGGAGACCGCACCGGCCGCCGCGC
TGGCTCATATGGCATTTCTTTTTCCACGATCGAGAGGTTGACGCGCGGCTTCGTGCTGTTGCTGCTCGGCGCAGTCCTCG
TCCTCGGAGCCCCGGCGCGGGCCGCCGACCCCGTCAGGATCGTCGTGCTCGGTGATTCCCTAAGCGCTGGCCTTGGGCTT
TCCGCCGCCGATGCATTTCCCGCGCAATTGGAGAAGGCGTTAAAAGACAAGGGTGTGAACGTCGCGATCAGCAATGCCGG
CGTCTCCGGCGACACCGCCTCCGGCGGTCTTGACCGGTTGGACTGGTCCGTTCCGGCGGGCACGCAGGGCGTCATCCTTG
AACTCGGCGCAAACGATGCCTTGCGCGGCATCGATCCCAAGGTGACGCGCGCCGCGCTGGATGAAATCCTCAAACGCCTG
AAGGCGCGCAACATCGCGATCCTGCTATGCGGCATGGTCGCGCCGCCGAACTACGGCGACGAATACGCGGCTCAATTCGG
CGCAATCTTTTCCGATCTCGCGAAAAAACATGGCGTCCCGTTATACCCGTTCTTTCTGGAAGGCGTTGCGGCGAATGCCG
AACTCAATCAGGCGGACGGAATTCATCCGACGGCGGCCGGGGTGACAACGATCGTCAAGAATATTCTTTCCACGGTTGAA
GCCTTTCTCGATACCCTTTCCGAGCGGAAGACTTGA

Upstream 100 bases:

>100_bases
TGGAGACGAGCGTTTGATGAGACTGTCCATGCCTGTTCCTGTGTTCGCCTATCCTGCCGCACCGTTCCGAGCGAACTTCG
GATTCAGAAGGACACGGAAA

Downstream 100 bases:

>100_bases
AGAAAGGGTGCGCATCCAGGCCACGGCTTGCACCGTCACAAAACTACGATAGAACGGCAGTTGTATCGTCGCCGGGCCGT
CACTCGATGGTTCCGACCGG

Product: lysophospholipase L1 and related esterase

Products: NA

Alternate protein names: Lecithinase B; Lysophospholipase L1; Protease I [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MWSRLLPYGRPPEHLKHNDGDRTGRRAGSYGISFSTIERLTRGFVLLLLGAVLVLGAPARAADPVRIVVLGDSLSAGLGL
SAADAFPAQLEKALKDKGVNVAISNAGVSGDTASGGLDRLDWSVPAGTQGVILELGANDALRGIDPKVTRAALDEILKRL
KARNIAILLCGMVAPPNYGDEYAAQFGAIFSDLAKKHGVPLYPFFLEGVAANAELNQADGIHPTAAGVTTIVKNILSTVE
AFLDTLSERKT

Sequences:

>Translated_251_residues
MWSRLLPYGRPPEHLKHNDGDRTGRRAGSYGISFSTIERLTRGFVLLLLGAVLVLGAPARAADPVRIVVLGDSLSAGLGL
SAADAFPAQLEKALKDKGVNVAISNAGVSGDTASGGLDRLDWSVPAGTQGVILELGANDALRGIDPKVTRAALDEILKRL
KARNIAILLCGMVAPPNYGDEYAAQFGAIFSDLAKKHGVPLYPFFLEGVAANAELNQADGIHPTAAGVTTIVKNILSTVE
AFLDTLSERKT
>Mature_251_residues
MWSRLLPYGRPPEHLKHNDGDRTGRRAGSYGISFSTIERLTRGFVLLLLGAVLVLGAPARAADPVRIVVLGDSLSAGLGL
SAADAFPAQLEKALKDKGVNVAISNAGVSGDTASGGLDRLDWSVPAGTQGVILELGANDALRGIDPKVTRAALDEILKRL
KARNIAILLCGMVAPPNYGDEYAAQFGAIFSDLAKKHGVPLYPFFLEGVAANAELNQADGIHPTAAGVTTIVKNILSTVE
AFLDTLSERKT

Specific function: Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=198, Percent_Identity=40.9090909090909, Blast_Score=127, Evalue=8e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 26365; Mature: 26365

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS01098 LIPASE_GDSL_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWSRLLPYGRPPEHLKHNDGDRTGRRAGSYGISFSTIERLTRGFVLLLLGAVLVLGAPAR
CCCCCCCCCCCHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
AADPVRIVVLGDSLSAGLGLSAADAFPAQLEKALKDKGVNVAISNAGVSGDTASGGLDRL
CCCCEEEEEECCCCCCCCCCCHHHCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCC
DWSVPAGTQGVILELGANDALRGIDPKVTRAALDEILKRLKARNIAILLCGMVAPPNYGD
CCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCH
EYAAQFGAIFSDLAKKHGVPLYPFFLEGVAANAELNQADGIHPTAAGVTTIVKNILSTVE
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
AFLDTLSERKT
HHHHHHHHCCC
>Mature Secondary Structure
MWSRLLPYGRPPEHLKHNDGDRTGRRAGSYGISFSTIERLTRGFVLLLLGAVLVLGAPAR
CCCCCCCCCCCHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
AADPVRIVVLGDSLSAGLGLSAADAFPAQLEKALKDKGVNVAISNAGVSGDTASGGLDRL
CCCCEEEEEECCCCCCCCCCCHHHCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCC
DWSVPAGTQGVILELGANDALRGIDPKVTRAALDEILKRLKARNIAILLCGMVAPPNYGD
CCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCH
EYAAQFGAIFSDLAKKHGVPLYPFFLEGVAANAELNQADGIHPTAAGVTTIVKNILSTVE
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
AFLDTLSERKT
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]