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The map label for this gene is ligT [C]

Identifier: 75674615

GI number: 75674615

Start: 467986

End: 468522

Strand: Direct

Name: ligT [C]

Synonym: Nwi_0417

Alternate gene names: 75674615

Gene position: 467986-468522 (Clockwise)

Preceding gene: 75674614

Following gene: 75674616

Centisome position: 13.76

GC content: 66.11

Gene sequence:

>537_bases
ATGCCGCGTTTGTTCACGGGGCTGGAAATACCGCCCGAGATCTGCCGCACGCTGTCCGGTCTGCGTGGCGGCTTGCCGGG
TGCCCGCTGGATCGATCCGGAGAACTATCACCTCACGCTCCGCTTCATCGGCGATATCGACGGCGCCGCCGCGAACGAGG
TCGCATCGGTGCTGCTCCGGGTCAATCGCAAGCCTTTCGAGGTGACGCTGCGGGGACTGTCGAGCTTCGGCGGCAGGAAA
CCGCGCGCCGTGGTCGCCTCGGTGGAGCCGAGCGGACCTCTGATCGAGTTGCAGGCCGAGCTTGAGCGGCTGATGCGGCG
CTGCGGGCTTGGCGCCGAGGGGCGGAAGTTCACGCCGCATATCACGCTGGCGCGCTTGCGCGACGCATCCAATCAGGATG
TCGCAAACTATCTGTCTGTTCGGGGTTATTTCCCGGCCACGGTATTCACCGCCGAGCGCTTTGTGCTGTTCTCCTCACGT
GCCTCGGTCGGCGGCGGCCCTTATGTCGTCGAGGACGCCTACGCGCTCGGCGCGTGA

Upstream 100 bases:

>100_bases
GGCCGTCACTCGATGGTTCCGACCGGGTGTCGGGACCGAAGCCATTCGTCGAGGATTTGCATCGAGGATTTGCATCGAGG
ATTTGCATCGAGGATTTGCG

Downstream 100 bases:

>100_bases
TCGCCGGTTCGCAAGACTTTCAACCAGTTTTCGACGCGCATCCGCCCTCTCAGGAGCCGTAACCTGTCCGATGCTGTCCT
CTCCTCCCCCATCGTTGCGG

Product: 2',5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 178; Mature: 177

Protein sequence:

>178_residues
MPRLFTGLEIPPEICRTLSGLRGGLPGARWIDPENYHLTLRFIGDIDGAAANEVASVLLRVNRKPFEVTLRGLSSFGGRK
PRAVVASVEPSGPLIELQAELERLMRRCGLGAEGRKFTPHITLARLRDASNQDVANYLSVRGYFPATVFTAERFVLFSSR
ASVGGGPYVVEDAYALGA

Sequences:

>Translated_178_residues
MPRLFTGLEIPPEICRTLSGLRGGLPGARWIDPENYHLTLRFIGDIDGAAANEVASVLLRVNRKPFEVTLRGLSSFGGRK
PRAVVASVEPSGPLIELQAELERLMRRCGLGAEGRKFTPHITLARLRDASNQDVANYLSVRGYFPATVFTAERFVLFSSR
ASVGGGPYVVEDAYALGA
>Mature_177_residues
PRLFTGLEIPPEICRTLSGLRGGLPGARWIDPENYHLTLRFIGDIDGAAANEVASVLLRVNRKPFEVTLRGLSSFGGRKP
RAVVASVEPSGPLIELQAELERLMRRCGLGAEGRKFTPHITLARLRDASNQDVANYLSVRGYFPATVFTAERFVLFSSRA
SVGGGPYVVEDAYALGA

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 19359; Mature: 19227

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLFTGLEIPPEICRTLSGLRGGLPGARWIDPENYHLTLRFIGDIDGAAANEVASVLLR
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHH
VNRKPFEVTLRGLSSFGGRKPRAVVASVEPSGPLIELQAELERLMRRCGLGAEGRKFTPH
CCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCC
ITLARLRDASNQDVANYLSVRGYFPATVFTAERFVLFSSRASVGGGPYVVEDAYALGA
EEEEEHHCCCCCHHHHHHHHCCCCCHHHHHHHEEEEEECCCCCCCCCEEEECCHHCCC
>Mature Secondary Structure 
PRLFTGLEIPPEICRTLSGLRGGLPGARWIDPENYHLTLRFIGDIDGAAANEVASVLLR
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHH
VNRKPFEVTLRGLSSFGGRKPRAVVASVEPSGPLIELQAELERLMRRCGLGAEGRKFTPH
CCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCC
ITLARLRDASNQDVANYLSVRGYFPATVFTAERFVLFSSRASVGGGPYVVEDAYALGA
EEEEEHHCCCCCHHHHHHHHCCCCCHHHHHHHEEEEEECCCCCCCCCEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]