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The map label for this gene is tusE [H]

Identifier: 74318676

GI number: 74318676

Start: 2733964

End: 2734296

Strand: Direct

Name: tusE [H]

Synonym: Tbd_2658

Alternate gene names: 74318676

Gene position: 2733964-2734296 (Clockwise)

Preceding gene: 74318675

Following gene: 74318679

Centisome position: 93.96

GC content: 59.76

Gene sequence:

>333_bases
ATGGAACGTGAAATCAACGGCAAGATGGTCGAAACCGACCCGGAAGGCTATCTGGTCAATCTCGAAGACTGGTCGGAGGA
GCTGGCCATCGAACTGGCCAAGGAAGACAAGCTGGAGCTCGGCGAGAACCACTGGAAGGTCATCCACTACATGCGCGACC
AGTTCGCGCAGAACGGCACCGCGCCCAACCTGCGTTTCCTGCAGAAGGGCCTGAAGGAAGAATTCGGCAACGAATGGGGC
GACAAGAAATTCCTTTTCGACCTCTTCCCCTTCGGCCCGGCGAAGCAGGCCGGACGCTACGCCGGAACGCCGAAGCCGAC
CGGCTGCGTGTGA

Upstream 100 bases:

>100_bases
GCAAGGTCGCGCGCAAGTAAGGCCCGGCAAAAAGTAGTTGACCAGTTTACTAGGTTATTCTATTGTTCGCGCTCGCTATT
TTTGGAGAGAGAGCGGAATC

Downstream 100 bases:

>100_bases
GCAAGGCAGCGAGCACCGTCTGAAAAAGCCCCGCTGCGCGGGGCTTTTTCATTGCGGCCGCGCTCCCGCGTCGTCCGGCG
CGCCGGCAAGCGCGTCGACG

Product: DsrC -like protein

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein E [H]

Number of amino acids: Translated: 110; Mature: 110

Protein sequence:

>110_residues
MEREINGKMVETDPEGYLVNLEDWSEELAIELAKEDKLELGENHWKVIHYMRDQFAQNGTAPNLRFLQKGLKEEFGNEWG
DKKFLFDLFPFGPAKQAGRYAGTPKPTGCV

Sequences:

>Translated_110_residues
MEREINGKMVETDPEGYLVNLEDWSEELAIELAKEDKLELGENHWKVIHYMRDQFAQNGTAPNLRFLQKGLKEEFGNEWG
DKKFLFDLFPFGPAKQAGRYAGTPKPTGCV
>Mature_110_residues
MEREINGKMVETDPEGYLVNLEDWSEELAIELAKEDKLELGENHWKVIHYMRDQFAQNGTAPNLRFLQKGLKEEFGNEWG
DKKFLFDLFPFGPAKQAGRYAGTPKPTGCV

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions. Could accept sulfur from tusD [H]

COG id: COG2920

COG function: function code P; Dissimilatory sulfite reductase (desulfoviridin), gamma subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dsrC/tusE family [H]

Homologues:

Organism=Escherichia coli, GI87081811, Length=104, Percent_Identity=44.2307692307692, Blast_Score=105, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007453 [H]

Pfam domain/function: PF04358 DsrC [H]

EC number: NA

Molecular weight: Translated: 12649; Mature: 12649

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEREINGKMVETDPEGYLVNLEDWSEELAIELAKEDKLELGENHWKVIHYMRDQFAQNGT
CCCCCCCCEEEECCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCC
APNLRFLQKGLKEEFGNEWGDKKFLFDLFPFGPAKQAGRYAGTPKPTGCV
CCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEREINGKMVETDPEGYLVNLEDWSEELAIELAKEDKLELGENHWKVIHYMRDQFAQNGT
CCCCCCCCEEEECCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCC
APNLRFLQKGLKEEFGNEWGDKKFLFDLFPFGPAKQAGRYAGTPKPTGCV
CCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA