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The map label for this gene is hemL

Identifier: 74318575

GI number: 74318575

Start: 2631203

End: 2632480

Strand: Direct

Name: hemL

Synonym: Tbd_2557

Alternate gene names: 74318575

Gene position: 2631203-2632480 (Clockwise)

Preceding gene: 74318574

Following gene: 74318576

Centisome position: 90.43

GC content: 68.15

Gene sequence:

>1278_bases
ATGAGCCGTAACGAAGCCCTATTCGAAGCATCGCAAAAAGTCATCCCGGGCGGGGTGAATTCGCCCGTGCGCGCGTTCCG
CAGCGTCGGCGGCACGCCGGTGTTCTTCAGCCGCGCCCGGGGCTCGCGCGTGTGGGACGCGGACGGCCGCGAGTACATCG
ATTACGTCGGCTCCTGGGGTCCCGCGATACTCGGCCACGCCCATCCGGGAACGGTCAAGGCGGTCCAGGACGCCGCTGCG
AACGGCCTGTCCTTTGGCGCACCGTCGGAGGCCGAACTCACGATCGCGACGCGCATCACCGAGCTTCTGCCGAGCGTCGA
GAAGGTGCGCCTCGTATCCTCGGGCACCGAAGCGACGATGAGCGCGATCCGGCTCGCCCGCGGCTTCACCGGTCGCAGCA
AGTTCATCAAGTTCGAAGGCTGCTACCACGGCCACGCCGACTTTCTGCTGGTCAAGGCCGGTTCGGGCGCACTGACCTTC
GGCAATCCGACTTCCGCCGGCGTGCCGCCCGAGGTCGCGGCGCAGACGATTGTTCTCGACTACAACGACGTCGCCGGCCT
CGAGCGCACTTTCGCCGAGATCGGCGACGAGATCGCCTGCATCATCGTCGAGCCCTTCGCCGGCAACATGAACCTCGTCA
AACCTAGCGCGGAGTTCATGGCCGCGATGCGCCGTCTTTGCGACGAGCACGGCGCGCTGCTGATCTTCGACGAAGTCATG
ACCGGTTTTCGCGTCGACCTCGGCTGCGCGCAGAAACTGCTCGGCATCCGCCCCGACCTCACGACGCTCGGCAAGGTCAT
CGGCGGCGGCATGCCCGTCGGCGCCTTCGGCGGGCGCGCCGACGTCATGGACGCGCTCGCGCCGGTGGGCCCGGTGTATC
AGGCCGGAACGCTGTCGGGCAACCCGGTCGCGGTGGCGGCGGGCCTCGCGACGCTCGCCTCGGTGTCCGAACCGGGCTTC
TACCCCGCGCTCGCGGCGCGCACCGAAAAGCTCGTCAAGGGCCTGACCGCTGCCGCGCGCGAGGCCGGCGTGACCTTCTG
CGCCGACTCGGTCGGCGGCATGTTCGGCCTCTATTTCGCGGCCGAGCCGCCCGCGAGCTTTGCCGAAGTCATGCGCTGCG
ACAAGGAGAAATTCAACCGCTTCTTCCATGCCATGCTCGACCACGGCGTTTATCTCGCGCCCTCGGCCTTCGAGGCCGGA
TTCGTTTCGGCCGCCCACAGCGACGCCGACATCGACGCGACCGTCGCCGCGGCGCGGGAAGTGTTCCGCACGCTATGA

Upstream 100 bases:

>100_bases
CTTGCGGTCCTCAGTGCACTGTTCGACGCGCCCGACGTACGCCGCGCCGCAGCCGAACTGAATCGATTATTTGCAGCCGA
ACCTGAAGAGTGACCGACCC

Downstream 100 bases:

>100_bases
ACGACGCGCTGTCCCCGGAAGACGAGCTCCGCCTGCACGTACTCTTCAACACCGAGCTGCGGGCGGTTCGCATCGACGAG
TCGAGCATGACCCTATGGGC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MSRNEALFEASQKVIPGGVNSPVRAFRSVGGTPVFFSRARGSRVWDADGREYIDYVGSWGPAILGHAHPGTVKAVQDAAA
NGLSFGAPSEAELTIATRITELLPSVEKVRLVSSGTEATMSAIRLARGFTGRSKFIKFEGCYHGHADFLLVKAGSGALTF
GNPTSAGVPPEVAAQTIVLDYNDVAGLERTFAEIGDEIACIIVEPFAGNMNLVKPSAEFMAAMRRLCDEHGALLIFDEVM
TGFRVDLGCAQKLLGIRPDLTTLGKVIGGGMPVGAFGGRADVMDALAPVGPVYQAGTLSGNPVAVAAGLATLASVSEPGF
YPALAARTEKLVKGLTAAAREAGVTFCADSVGGMFGLYFAAEPPASFAEVMRCDKEKFNRFFHAMLDHGVYLAPSAFEAG
FVSAAHSDADIDATVAAAREVFRTL

Sequences:

>Translated_425_residues
MSRNEALFEASQKVIPGGVNSPVRAFRSVGGTPVFFSRARGSRVWDADGREYIDYVGSWGPAILGHAHPGTVKAVQDAAA
NGLSFGAPSEAELTIATRITELLPSVEKVRLVSSGTEATMSAIRLARGFTGRSKFIKFEGCYHGHADFLLVKAGSGALTF
GNPTSAGVPPEVAAQTIVLDYNDVAGLERTFAEIGDEIACIIVEPFAGNMNLVKPSAEFMAAMRRLCDEHGALLIFDEVM
TGFRVDLGCAQKLLGIRPDLTTLGKVIGGGMPVGAFGGRADVMDALAPVGPVYQAGTLSGNPVAVAAGLATLASVSEPGF
YPALAARTEKLVKGLTAAAREAGVTFCADSVGGMFGLYFAAEPPASFAEVMRCDKEKFNRFFHAMLDHGVYLAPSAFEAG
FVSAAHSDADIDATVAAAREVFRTL
>Mature_424_residues
SRNEALFEASQKVIPGGVNSPVRAFRSVGGTPVFFSRARGSRVWDADGREYIDYVGSWGPAILGHAHPGTVKAVQDAAAN
GLSFGAPSEAELTIATRITELLPSVEKVRLVSSGTEATMSAIRLARGFTGRSKFIKFEGCYHGHADFLLVKAGSGALTFG
NPTSAGVPPEVAAQTIVLDYNDVAGLERTFAEIGDEIACIIVEPFAGNMNLVKPSAEFMAAMRRLCDEHGALLIFDEVMT
GFRVDLGCAQKLLGIRPDLTTLGKVIGGGMPVGAFGGRADVMDALAPVGPVYQAGTLSGNPVAVAAGLATLASVSEPGFY
PALAARTEKLVKGLTAAAREAGVTFCADSVGGMFGLYFAAEPPASFAEVMRCDKEKFNRFFHAMLDHGVYLAPSAFEAGF
VSAAHSDADIDATVAAAREVFRTL

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=316, Percent_Identity=29.4303797468354, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI284507298, Length=224, Percent_Identity=30.3571428571429, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=63.1455399061033, Blast_Score=547, Evalue=1e-157,
Organism=Escherichia coli, GI1789016, Length=359, Percent_Identity=34.5403899721448, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1789759, Length=316, Percent_Identity=30.0632911392405, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1788044, Length=339, Percent_Identity=30.6784660766962, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1787560, Length=363, Percent_Identity=31.6804407713499, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI145693181, Length=340, Percent_Identity=30, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786991, Length=386, Percent_Identity=27.979274611399, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI25144271, Length=353, Percent_Identity=26.628895184136, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71992977, Length=327, Percent_Identity=28.4403669724771, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI25144274, Length=191, Percent_Identity=27.7486910994764, Blast_Score=68, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6323470, Length=307, Percent_Identity=30.2931596091205, Blast_Score=106, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6324432, Length=362, Percent_Identity=25.1381215469613, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21357415, Length=297, Percent_Identity=27.6094276094276, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI21356575, Length=311, Percent_Identity=27.3311897106109, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI161085790, Length=327, Percent_Identity=25.0764525993884, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28574759, Length=327, Percent_Identity=25.0764525993884, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_THIDA (Q3SFU6)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_316315.1
- ProteinModelPortal:   Q3SFU6
- SMR:   Q3SFU6
- STRING:   Q3SFU6
- GeneID:   3672533
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_2557
- NMPDR:   fig|292415.3.peg.2630
- eggNOG:   COG0001
- HOGENOM:   HBG725944
- OMA:   VGCFGGK
- PhylomeDB:   Q3SFU6
- BioCyc:   TDEN292415:TBD_2557-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 44493; Mature: 44362

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRNEALFEASQKVIPGGVNSPVRAFRSVGGTPVFFSRARGSRVWDADGREYIDYVGSWG
CCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEECCCCCEEECCCCHHHHHHHCCCC
PAILGHAHPGTVKAVQDAAANGLSFGAPSEAELTIATRITELLPSVEKVRLVSSGTEATM
CHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHCCCHHHHH
SAIRLARGFTGRSKFIKFEGCYHGHADFLLVKAGSGALTFGNPTSAGVPPEVAAQTIVLD
HHHHHHHCCCCCCCEEEEECEECCCCCEEEEEECCCEEECCCCCCCCCCHHHHHEEEEEE
YNDVAGLERTFAEIGDEIACIIVEPFAGNMNLVKPSAEFMAAMRRLCDEHGALLIFDEVM
CCHHHHHHHHHHHCCCCEEEEEEECCCCCCEEECCCHHHHHHHHHHHHHCCCEEEEHHHH
TGFRVDLGCAQKLLGIRPDLTTLGKVIGGGMPVGAFGGRADVMDALAPVGPVYQAGTLSG
CCCEECHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCC
NPVAVAAGLATLASVSEPGFYPALAARTEKLVKGLTAAAREAGVTFCADSVGGMFGLYFA
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHCCCEEEEEE
AEPPASFAEVMRCDKEKFNRFFHAMLDHGVYLAPSAFEAGFVSAAHSDADIDATVAAARE
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHCCCCCCCCHHHHHHHHH
VFRTL
HHHHC
>Mature Secondary Structure 
SRNEALFEASQKVIPGGVNSPVRAFRSVGGTPVFFSRARGSRVWDADGREYIDYVGSWG
CCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEECCCCCEEECCCCHHHHHHHCCCC
PAILGHAHPGTVKAVQDAAANGLSFGAPSEAELTIATRITELLPSVEKVRLVSSGTEATM
CHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHCCCHHHHH
SAIRLARGFTGRSKFIKFEGCYHGHADFLLVKAGSGALTFGNPTSAGVPPEVAAQTIVLD
HHHHHHHCCCCCCCEEEEECEECCCCCEEEEEECCCEEECCCCCCCCCCHHHHHEEEEEE
YNDVAGLERTFAEIGDEIACIIVEPFAGNMNLVKPSAEFMAAMRRLCDEHGALLIFDEVM
CCHHHHHHHHHHHCCCCEEEEEEECCCCCCEEECCCHHHHHHHHHHHHHCCCEEEEHHHH
TGFRVDLGCAQKLLGIRPDLTTLGKVIGGGMPVGAFGGRADVMDALAPVGPVYQAGTLSG
CCCEECHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCC
NPVAVAAGLATLASVSEPGFYPALAARTEKLVKGLTAAAREAGVTFCADSVGGMFGLYFA
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHCCCEEEEEE
AEPPASFAEVMRCDKEKFNRFFHAMLDHGVYLAPSAFEAGFVSAAHSDADIDATVAAARE
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHCCCCCCCCHHHHHHHHH
VFRTL
HHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA