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The map label for this gene is thiE

Identifier: 74318574

GI number: 74318574

Start: 2630560

End: 2631195

Strand: Direct

Name: thiE

Synonym: Tbd_2556

Alternate gene names: 74318574

Gene position: 2630560-2631195 (Clockwise)

Preceding gene: 74318573

Following gene: 74318575

Centisome position: 90.4

GC content: 72.17

Gene sequence:

>636_bases
TTGCGCACCTTCCCCGGCGGCGTATACGCCCTCACCCGCGAGACCCCTGACACCGAGCGGCTGCTCGCCGAAGTCGAAGC
CGCGCTCGCGGGCGGCGTCGCGGCAGTCCAGTACCGCGACAAGTCCGGCGACGTCGCGCAGCGGCACGCGCAGGCGAGCG
AACTCGCCGCCCTGTGCCGGCGCTTCGGGGTGCCGCTGATCGTCAATGACGACCTGCGGCTGGCCGACCTCGCCGGCGCC
GACGGCGTGCATCTCGGGCGCGACGACGCCAGCATCCGCGAGGCGCGCATCATTCTCGGACCGGCGAAGTTCGTCGGCGC
ATCGTGTTACCAGAGCCTCGACCTCGCACGCGCGGCGCAGACCGCGGGCGCGGACTATGTCGCGTTCGGCAGTTTCTACC
CCTCGCCGACCAAGCCCGCCGCGGCGCGCGCCGACGTCGCGCTGCTCGCGCGGGCGAGCCGGCACATCGCGCTGCCGGTC
GTCGCGATCGGCGGCATCACGGCGGCCAACGCCGCGCCGCTCATCGACGCGGGCGCCGACAGCCTTGCGGTCCTCAGTGC
ACTGTTCGACGCGCCCGACGTACGCCGCGCCGCAGCCGAACTGAATCGATTATTTGCAGCCGAACCTGAAGAGTGA

Upstream 100 bases:

>100_bases
CTACGAAGCGCTCAAGGCGGCATTCCGCCCCGGCATGGGGCAGTACATCCCCGAACGCTTCTTCTGGGCGCAGGACGCAC
AGGCGGAAGGGAACGGCTGA

Downstream 100 bases:

>100_bases
CCGACCCATGAGCCGTAACGAAGCCCTATTCGAAGCATCGCAAAAAGTCATCCCGGGCGGGGTGAATTCGCCCGTGCGCG
CGTTCCGCAGCGTCGGCGGC

Product: thiamine monophosphate synthase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MRTFPGGVYALTRETPDTERLLAEVEAALAGGVAAVQYRDKSGDVAQRHAQASELAALCRRFGVPLIVNDDLRLADLAGA
DGVHLGRDDASIREARIILGPAKFVGASCYQSLDLARAAQTAGADYVAFGSFYPSPTKPAAARADVALLARASRHIALPV
VAIGGITAANAAPLIDAGADSLAVLSALFDAPDVRRAAAELNRLFAAEPEE

Sequences:

>Translated_211_residues
MRTFPGGVYALTRETPDTERLLAEVEAALAGGVAAVQYRDKSGDVAQRHAQASELAALCRRFGVPLIVNDDLRLADLAGA
DGVHLGRDDASIREARIILGPAKFVGASCYQSLDLARAAQTAGADYVAFGSFYPSPTKPAAARADVALLARASRHIALPV
VAIGGITAANAAPLIDAGADSLAVLSALFDAPDVRRAAAELNRLFAAEPEE
>Mature_211_residues
MRTFPGGVYALTRETPDTERLLAEVEAALAGGVAAVQYRDKSGDVAQRHAQASELAALCRRFGVPLIVNDDLRLADLAGA
DGVHLGRDDASIREARIILGPAKFVGASCYQSLDLARAAQTAGADYVAFGSFYPSPTKPAAARADVALLARASRHIALPV
VAIGGITAANAAPLIDAGADSLAVLSALFDAPDVRRAAAELNRLFAAEPEE

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=200, Percent_Identity=33.5, Blast_Score=80, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6325042, Length=220, Percent_Identity=30, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_THIDA (Q3SFU7)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_316314.1
- ProteinModelPortal:   Q3SFU7
- STRING:   Q3SFU7
- GeneID:   3672532
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_2556
- NMPDR:   fig|292415.3.peg.2629
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   IMGSNNC
- PhylomeDB:   Q3SFU7
- BioCyc:   TDEN292415:TBD_2556-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 21891; Mature: 21891

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTFPGGVYALTRETPDTERLLAEVEAALAGGVAAVQYRDKSGDVAQRHAQASELAALCR
CCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
RFGVPLIVNDDLRLADLAGADGVHLGRDDASIREARIILGPAKFVGASCYQSLDLARAAQ
HCCCCEEECCCCEEEECCCCCCCEECCCCCHHHHEEEEECCHHHHHHHHHHHHHHHHHHH
TAGADYVAFGSFYPSPTKPAAARADVALLARASRHIALPVVAIGGITAANAAPLIDAGAD
HCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCEECCCCCCCCCCCCH
SLAVLSALFDAPDVRRAAAELNRLFAAEPEE
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRTFPGGVYALTRETPDTERLLAEVEAALAGGVAAVQYRDKSGDVAQRHAQASELAALCR
CCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
RFGVPLIVNDDLRLADLAGADGVHLGRDDASIREARIILGPAKFVGASCYQSLDLARAAQ
HCCCCEEECCCCEEEECCCCCCCEECCCCCHHHHEEEEECCHHHHHHHHHHHHHHHHHHH
TAGADYVAFGSFYPSPTKPAAARADVALLARASRHIALPVVAIGGITAANAAPLIDAGAD
HCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCEECCCCCCCCCCCCH
SLAVLSALFDAPDVRRAAAELNRLFAAEPEE
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA