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The map label for this gene is hemK [H]

Identifier: 74318512

GI number: 74318512

Start: 2567450

End: 2568313

Strand: Direct

Name: hemK [H]

Synonym: Tbd_2494

Alternate gene names: 74318512

Gene position: 2567450-2568313 (Clockwise)

Preceding gene: 74318511

Following gene: 74318513

Centisome position: 88.23

GC content: 72.57

Gene sequence:

>864_bases
ATGACCGGGCACGCGGCCACCGTCGCTGGCCTGCTCGACGTCGCGACCGACCGAGTCGGCCGCGCGCTCGGGCTCGACAA
GCGCGAAGCCCGGCTCGAAGCCCGCGTGCTCGCCGCCCACGCCTGGCAGGTGACGCCGGCCTGGCTCATCGCGCACGACA
CCGATGTGGCGACGGCGCCGCAAACCGCCGCCTTCGACGCGCTGCTCGCCCGGCGCCTGGGCGGCGAACCGATCGCCTAC
ATCGTCGGCGCGCGCGAGTTCTACGGGCGCAGGTTCGAAGTATCGCCCGCGGTATTGATTCCACGGCCGGAGACCGAACT
CCTCGTCGAGTTGGCGCTCGCACGGATCCCGCCCGACGAGACGACCACGGTGCTCGACCTCGGCACCGGCAGCGGCTGCA
TCGCGATCACGCTGGCGCTGGAGCGCTCGCGGGCGCGCGTGACCGCGGTCGAGCGCTCGCCAGCTGCGCTGGCGCTCGCC
CGCCGCAACGCGCAAACGCTCGGCGCCAGCGTCGAATTTTCGGCCGGCGACTGGTTCAGCGACCTGACGGGGCGCAGCTA
CGACCTCATCGTCGCGAACCCGCCCTACATCGCCGACGCCGACCCGCATCTCGCCCGAGGCGACCTGCGCCACGAACCGG
TCGCCGCGCTGGCCTCTGGTGCGGATGGACTCGACGAGCTGCGGCGGCTGATCGACGGGGCGCGCGACCACCTGCGGCGC
CCTGGAATGCTGTTGCTGGAGCACGGCTACGACCAGGCCGACGCGGTGCATGCGCTGCTTCGCAGCGGCGGCTTCGCCCG
CCCGCGCTGCTGGCATGACCTCGCCGGCAACCCGCGCGTGAGCGGCGCGGAATTGTCGAAATAA

Upstream 100 bases:

>100_bases
CGATGCGGTGATGGACGGCGACCTCGGCGAACTGCTCGACGCGCTCGCCGCCGAGCACCAGGCCGCGCAGCTGGCGACCT
TGTCCGGCGAGGGCTGAGCG

Downstream 100 bases:

>100_bases
TTTACAGGTGCCCGGCATGGCTTGCGTCCAGCCCACCGCCCCGCACGCGCCCGCGGGCTTCGCCAGCCGGCATCGAGATG
CTGGTGCGATTCTTGCTGGT

Product: modification methylase HemK

Products: NA

Alternate protein names: M.XfaHemK2P [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MTGHAATVAGLLDVATDRVGRALGLDKREARLEARVLAAHAWQVTPAWLIAHDTDVATAPQTAAFDALLARRLGGEPIAY
IVGAREFYGRRFEVSPAVLIPRPETELLVELALARIPPDETTTVLDLGTGSGCIAITLALERSRARVTAVERSPAALALA
RRNAQTLGASVEFSAGDWFSDLTGRSYDLIVANPPYIADADPHLARGDLRHEPVAALASGADGLDELRRLIDGARDHLRR
PGMLLLEHGYDQADAVHALLRSGGFARPRCWHDLAGNPRVSGAELSK

Sequences:

>Translated_287_residues
MTGHAATVAGLLDVATDRVGRALGLDKREARLEARVLAAHAWQVTPAWLIAHDTDVATAPQTAAFDALLARRLGGEPIAY
IVGAREFYGRRFEVSPAVLIPRPETELLVELALARIPPDETTTVLDLGTGSGCIAITLALERSRARVTAVERSPAALALA
RRNAQTLGASVEFSAGDWFSDLTGRSYDLIVANPPYIADADPHLARGDLRHEPVAALASGADGLDELRRLIDGARDHLRR
PGMLLLEHGYDQADAVHALLRSGGFARPRCWHDLAGNPRVSGAELSK
>Mature_286_residues
TGHAATVAGLLDVATDRVGRALGLDKREARLEARVLAAHAWQVTPAWLIAHDTDVATAPQTAAFDALLARRLGGEPIAYI
VGAREFYGRRFEVSPAVLIPRPETELLVELALARIPPDETTTVLDLGTGSGCIAITLALERSRARVTAVERSPAALALAR
RNAQTLGASVEFSAGDWFSDLTGRSYDLIVANPPYIADADPHLARGDLRHEPVAALASGADGLDELRRLIDGARDHLRRP
GMLLLEHGYDQADAVHALLRSGGFARPRCWHDLAGNPRVSGAELSK

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=249, Percent_Identity=30.5220883534137, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1787463, Length=252, Percent_Identity=43.2539682539683, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI87082085, Length=175, Percent_Identity=40.5714285714286, Blast_Score=114, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24582226, Length=210, Percent_Identity=32.3809523809524, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 30729; Mature: 30598

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGHAATVAGLLDVATDRVGRALGLDKREARLEARVLAAHAWQVTPAWLIAHDTDVATAP
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCCEEEEEECCCCCCCC
QTAAFDALLARRLGGEPIAYIVGAREFYGRRFEVSPAVLIPRPETELLVELALARIPPDE
CHHHHHHHHHHHHCCCCEEEEEECHHHHCCEEECCCEEEECCCCHHHHHHHHHHHCCCCC
TTTVLDLGTGSGCIAITLALERSRARVTAVERSPAALALARRNAQTLGASVEFSAGDWFS
CEEEEEECCCCCEEEEEEEEECCCCEEEEECCCCHHHHHHHHCHHHHCCEEEECCCCCHH
DLTGRSYDLIVANPPYIADADPHLARGDLRHEPVAALASGADGLDELRRLIDGARDHLRR
HCCCCCEEEEEECCCEECCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCC
PGMLLLEHGYDQADAVHALLRSGGFARPRCWHDLAGNPRVSGAELSK
CCEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TGHAATVAGLLDVATDRVGRALGLDKREARLEARVLAAHAWQVTPAWLIAHDTDVATAP
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCCEEEEEECCCCCCCC
QTAAFDALLARRLGGEPIAYIVGAREFYGRRFEVSPAVLIPRPETELLVELALARIPPDE
CHHHHHHHHHHHHCCCCEEEEEECHHHHCCEEECCCEEEECCCCHHHHHHHHHHHCCCCC
TTTVLDLGTGSGCIAITLALERSRARVTAVERSPAALALARRNAQTLGASVEFSAGDWFS
CEEEEEECCCCCEEEEEEEEECCCCEEEEECCCCHHHHHHHHCHHHHCCEEEECCCCCHH
DLTGRSYDLIVANPPYIADADPHLARGDLRHEPVAALASGADGLDELRRLIDGARDHLRR
HCCCCCEEEEEECCCEECCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCC
PGMLLLEHGYDQADAVHALLRSGGFARPRCWHDLAGNPRVSGAELSK
CCEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]