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The map label for this gene is prfA

Identifier: 74318511

GI number: 74318511

Start: 2566364

End: 2567446

Strand: Direct

Name: prfA

Synonym: Tbd_2493

Alternate gene names: 74318511

Gene position: 2566364-2567446 (Clockwise)

Preceding gene: 74318510

Following gene: 74318512

Centisome position: 88.2

GC content: 67.31

Gene sequence:

>1083_bases
ATGAAGGCGACGCTCGCCGACAAGCTCGCGCGCGCCGACGAGCGACTCGAAGAGCTCGACGCCCTCCTCGCGCAGCCCGA
GGTCGCGGCGGACATGGACAGTTATCGCAAGCTCACACGCGAGCACGCCGAGCTGTCGCCGGTGGTGGGCCTGTACCGCC
AGTACAAGCAGGTCGAGGCCGACCAGAAGACCGCGCAGGAGATGCTCGCCGACGCCGACATGCGCGAGCTCGCCGAGGCC
GAGCTCGCCGACGGTGCGGCACGCATCACCGAGCTCGAAAACGAACTACAAACCGCGCTGCTGCCGCGCGACCCCAACGA
CGAACGCAACATCTTCCTCGAGATTCGCGCCGGCACAGGCGGCGACGAGTCGGCACTGTTCGCCGGCAACCTGCTGCGCA
TGTACACGCGCTACGCCGAGCGCCAGCGCTGGAAGGTCGAAATCGTGTCCGAGAGTCCCGGCGAAGTCGGCGGCTACAAG
GAAGTCATCGTGCGGATCGTCGGCGAGGGCGCTTATTCGCGCTTGAAATTCGAGTCCGGCGGACATCGCGTGCAACGCGT
GCCGGAGACCGAATCGCAGGGCCGCATCCATACCTCGGCCTGCACGGTCGCCGTGATGCCCGAAGCCGCAGAGGTCGGCG
AGGTCGACATCAATCCGGCAGACCTGCGCATCGACACCTTCCGCGCCTCGGGTGCGGGCGGCCAGCACATCAACAAAACC
GATTCCGCGGTCCGCATCACTCACCTGCCGACCGGCCTCGTGGTCGAATGCCAGGACGACCGCTCCCAGCATCGCAACCG
CGCACAGGCCATGAGCGTGCTTGCGGCGCGGCTCAAGGACCGCGAAATCCAGGCCCAGCAGGCGAGTGAGGCGAGCACCC
GCAAAAGCCTGATCGGCAGCGGCGACCGCTCGGACCGCATCCGCACCTACAACTTCCCGCAGGGCCGCATCACCGACCAC
CGCATCAATCTCACGCTCTACAAGATCGATGCGGTGATGGACGGCGACCTCGGCGAACTGCTCGACGCGCTCGCCGCCGA
GCACCAGGCCGCGCAGCTGGCGACCTTGTCCGGCGAGGGCTGA

Upstream 100 bases:

>100_bases
AATTCCTGCACGCGCCGACCCACGGCCTGAGCCACGCCACGCCCGAGGAGCGCGCCGAACTGGTCCGCCTGATCAGCCAG
CTCTACGGGCTGCACCCGGA

Downstream 100 bases:

>100_bases
GCGATGACCGGGCACGCGGCCACCGTCGCTGGCCTGCTCGACGTCGCGACCGACCGAGTCGGCCGCGCGCTCGGGCTCGA
CAAGCGCGAAGCCCGGCTCG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKATLADKLARADERLEELDALLAQPEVAADMDSYRKLTREHAELSPVVGLYRQYKQVEADQKTAQEMLADADMRELAEA
ELADGAARITELENELQTALLPRDPNDERNIFLEIRAGTGGDESALFAGNLLRMYTRYAERQRWKVEIVSESPGEVGGYK
EVIVRIVGEGAYSRLKFESGGHRVQRVPETESQGRIHTSACTVAVMPEAAEVGEVDINPADLRIDTFRASGAGGQHINKT
DSAVRITHLPTGLVVECQDDRSQHRNRAQAMSVLAARLKDREIQAQQASEASTRKSLIGSGDRSDRIRTYNFPQGRITDH
RINLTLYKIDAVMDGDLGELLDALAAEHQAAQLATLSGEG

Sequences:

>Translated_360_residues
MKATLADKLARADERLEELDALLAQPEVAADMDSYRKLTREHAELSPVVGLYRQYKQVEADQKTAQEMLADADMRELAEA
ELADGAARITELENELQTALLPRDPNDERNIFLEIRAGTGGDESALFAGNLLRMYTRYAERQRWKVEIVSESPGEVGGYK
EVIVRIVGEGAYSRLKFESGGHRVQRVPETESQGRIHTSACTVAVMPEAAEVGEVDINPADLRIDTFRASGAGGQHINKT
DSAVRITHLPTGLVVECQDDRSQHRNRAQAMSVLAARLKDREIQAQQASEASTRKSLIGSGDRSDRIRTYNFPQGRITDH
RINLTLYKIDAVMDGDLGELLDALAAEHQAAQLATLSGEG
>Mature_360_residues
MKATLADKLARADERLEELDALLAQPEVAADMDSYRKLTREHAELSPVVGLYRQYKQVEADQKTAQEMLADADMRELAEA
ELADGAARITELENELQTALLPRDPNDERNIFLEIRAGTGGDESALFAGNLLRMYTRYAERQRWKVEIVSESPGEVGGYK
EVIVRIVGEGAYSRLKFESGGHRVQRVPETESQGRIHTSACTVAVMPEAAEVGEVDINPADLRIDTFRASGAGGQHINKT
DSAVRITHLPTGLVVECQDDRSQHRNRAQAMSVLAARLKDREIQAQQASEASTRKSLIGSGDRSDRIRTYNFPQGRITDH
RINLTLYKIDAVMDGDLGELLDALAAEHQAAQLATLSGEG

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=276, Percent_Identity=49.6376811594203, Blast_Score=279, Evalue=3e-75,
Organism=Homo sapiens, GI34577120, Length=328, Percent_Identity=41.1585365853659, Blast_Score=251, Evalue=5e-67,
Organism=Homo sapiens, GI166795305, Length=181, Percent_Identity=50.828729281768, Blast_Score=191, Evalue=9e-49,
Organism=Escherichia coli, GI1787462, Length=357, Percent_Identity=63.5854341736695, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI2367172, Length=335, Percent_Identity=41.1940298507463, Blast_Score=234, Evalue=9e-63,
Organism=Caenorhabditis elegans, GI17542784, Length=327, Percent_Identity=34.2507645259939, Blast_Score=180, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6321295, Length=316, Percent_Identity=42.7215189873418, Blast_Score=256, Evalue=3e-69,
Organism=Drosophila melanogaster, GI19921226, Length=334, Percent_Identity=41.3173652694611, Blast_Score=228, Evalue=4e-60,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_THIDA (Q3SG10)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_316251.1
- HSSP:   P0A7I0
- ProteinModelPortal:   Q3SG10
- SMR:   Q3SG10
- STRING:   Q3SG10
- GeneID:   3671812
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_2493
- NMPDR:   fig|292415.3.peg.2566
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- PhylomeDB:   Q3SG10
- ProtClustDB:   PRK00591
- BioCyc:   TDEN292415:TBD_2493-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 39693; Mature: 39693

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKATLADKLARADERLEELDALLAQPEVAADMDSYRKLTREHAELSPVVGLYRQYKQVEA
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DQKTAQEMLADADMRELAEAELADGAARITELENELQTALLPRDPNDERNIFLEIRAGTG
HHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCC
GDESALFAGNLLRMYTRYAERQRWKVEIVSESPGEVGGYKEVIVRIVGEGAYSRLKFESG
CCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHCCCCHHEEEECCC
GHRVQRVPETESQGRIHTSACTVAVMPEAAEVGEVDINPADLRIDTFRASGAGGQHINKT
CCHHHCCCCCCCCCEEEECEEEEEECCCCHHCCCCCCCCCCEEEEEEECCCCCCCCCCCC
DSAVRITHLPTGLVVECQDDRSQHRNRAQAMSVLAARLKDREIQAQQASEASTRKSLIGS
CCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDRSDRIRTYNFPQGRITDHRINLTLYKIDAVMDGDLGELLDALAAEHQAAQLATLSGEG
CCCCCCEEEEECCCCCCCCEEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKATLADKLARADERLEELDALLAQPEVAADMDSYRKLTREHAELSPVVGLYRQYKQVEA
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DQKTAQEMLADADMRELAEAELADGAARITELENELQTALLPRDPNDERNIFLEIRAGTG
HHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCC
GDESALFAGNLLRMYTRYAERQRWKVEIVSESPGEVGGYKEVIVRIVGEGAYSRLKFESG
CCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHCCCCHHEEEECCC
GHRVQRVPETESQGRIHTSACTVAVMPEAAEVGEVDINPADLRIDTFRASGAGGQHINKT
CCHHHCCCCCCCCCEEEECEEEEEECCCCHHCCCCCCCCCCEEEEEEECCCCCCCCCCCC
DSAVRITHLPTGLVVECQDDRSQHRNRAQAMSVLAARLKDREIQAQQASEASTRKSLIGS
CCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDRSDRIRTYNFPQGRITDHRINLTLYKIDAVMDGDLGELLDALAAEHQAAQLATLSGEG
CCCCCCEEEEECCCCCCCCEEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA