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The map label for this gene is hmeA [H]

Identifier: 74318493

GI number: 74318493

Start: 2551332

End: 2552111

Strand: Reverse

Name: hmeA [H]

Synonym: Tbd_2475

Alternate gene names: 74318493

Gene position: 2552111-2551332 (Counterclockwise)

Preceding gene: 74318494

Following gene: 74318492

Centisome position: 87.71

GC content: 66.54

Gene sequence:

>780_bases
ATGAGCGAATTGAAACCGATGTCTGACAACACGCCCGAGTCGGCGGAGCGCCGCCGGGTGATCGGCGGGCTCGCCGCCGC
CGCGGCCGGTCTTGCCATCGCGCCGGGGATCAAGCTGATCGAGGTCGCACAGGCACGTCCGGAGGAGCAGGGCGCGAGCA
GCGCCGTGCGCTGGGGCATGCTGGTCGACGCGACCAAGTGTGCGACCGGCTGTGATGACTGCGTGTCCGCGTGCAGCACC
GAAAACGGCTGGACGCCGGTCGCCGAGAGCGCCAAGCCGCGGCAGGCGCCTCAGTGGATACGCAAGCTCGAGCTGCAGGA
TCCGCTGACGCAGATGGAAACCAATCTGCCGATGATGTGCCAGCATTGCGCCGAGCCGCCGTGCGTCGACGTCTGCCCGA
CCGGCGCGTCGTTCAAGCGTGCCGACGGCATCGTGCTCGTCAACCGCCACACCTGTATCGGCTGCCGCTACTGCATGATG
GCGTGTCCTTACAAGGCACGCTCCCTCGTGCACGAGCCCCTCAACGACACGCAGAAGGCCGACGTACCGCGCGGCGTCGG
TTGCGTCGAGTCCTGCTCACTGTGCGTGCAGAAGGTCGATCGCGGCGACGGCACCACGGCGTGCGCCGAAGCCTGCTCCG
CGGCCGGTCATAACGCGATCGTCTTCGGCGACCTGAACGACCCCGATAGCGAGATCTCGAAGCGTTTGCGCGAACTGCCC
ACCAAGCAGGTGCGTGCCGACCTCAAGCTCAATACCGGCGTTCGCTATCACGGAATCTAA

Upstream 100 bases:

>100_bases
CACAGCATGCGCTGAACGCGGAAACCGCGCATTACAGGCACAAGCTGGCGGCCCAGGCCAACAGCAAGGTGAGCGCAGAA
GAGATGGCCGGGATTGTCCA

Downstream 100 bases:

>100_bases
AATAATATGGCAAGCATTACCTTCCGCGAAGTCGAGGGCAGCAGCCTCAAGTACTGGCTGCTCCTGGGTGGCCTCGGCCT
CTTCGTCCTGTTCGGGGCGC

Product: iron-sulfur cluster protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Hme subunit A [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MSELKPMSDNTPESAERRRVIGGLAAAAAGLAIAPGIKLIEVAQARPEEQGASSAVRWGMLVDATKCATGCDDCVSACST
ENGWTPVAESAKPRQAPQWIRKLELQDPLTQMETNLPMMCQHCAEPPCVDVCPTGASFKRADGIVLVNRHTCIGCRYCMM
ACPYKARSLVHEPLNDTQKADVPRGVGCVESCSLCVQKVDRGDGTTACAEACSAAGHNAIVFGDLNDPDSEISKRLRELP
TKQVRADLKLNTGVRYHGI

Sequences:

>Translated_259_residues
MSELKPMSDNTPESAERRRVIGGLAAAAAGLAIAPGIKLIEVAQARPEEQGASSAVRWGMLVDATKCATGCDDCVSACST
ENGWTPVAESAKPRQAPQWIRKLELQDPLTQMETNLPMMCQHCAEPPCVDVCPTGASFKRADGIVLVNRHTCIGCRYCMM
ACPYKARSLVHEPLNDTQKADVPRGVGCVESCSLCVQKVDRGDGTTACAEACSAAGHNAIVFGDLNDPDSEISKRLRELP
TKQVRADLKLNTGVRYHGI
>Mature_258_residues
SELKPMSDNTPESAERRRVIGGLAAAAAGLAIAPGIKLIEVAQARPEEQGASSAVRWGMLVDATKCATGCDDCVSACSTE
NGWTPVAESAKPRQAPQWIRKLELQDPLTQMETNLPMMCQHCAEPPCVDVCPTGASFKRADGIVLVNRHTCIGCRYCMMA
CPYKARSLVHEPLNDTQKADVPRGVGCVESCSLCVQKVDRGDGTTACAEACSAAGHNAIVFGDLNDPDSEISKRLRELPT
KQVRADLKLNTGVRYHGI

Specific function: Has menaquinol-oxidizing activity. HmeA, hmeB and hmeE subunits may together catalyze electron transfer from menaquinol to cytochrome c [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=213, Percent_Identity=35.6807511737089, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI226510944, Length=216, Percent_Identity=30.5555555555556, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1789370, Length=277, Percent_Identity=28.158844765343, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787122, Length=186, Percent_Identity=31.7204301075269, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787872, Length=174, Percent_Identity=31.0344827586207, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787478, Length=98, Percent_Identity=37.7551020408163, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787740, Length=98, Percent_Identity=37.7551020408163, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790326, Length=197, Percent_Identity=30.4568527918782, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 27741; Mature: 27610

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
9.7 %Cys+Met (Translated Protein)
6.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
9.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELKPMSDNTPESAERRRVIGGLAAAAAGLAIAPGIKLIEVAQARPEEQGASSAVRWGM
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHCCHHHHHHHHH
LVDATKCATGCDDCVSACSTENGWTPVAESAKPRQAPQWIRKLELQDPLTQMETNLPMMC
HHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHCCCHHHH
QHCAEPPCVDVCPTGASFKRADGIVLVNRHTCIGCRYCMMACPYKARSLVHEPLNDTQKA
HHCCCCCCCEECCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHC
DVPRGVGCVESCSLCVQKVDRGDGTTACAEACSAAGHNAIVFGDLNDPDSEISKRLRELP
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCC
TKQVRADLKLNTGVRYHGI
HHHHHHHHEECCCEEECCC
>Mature Secondary Structure 
SELKPMSDNTPESAERRRVIGGLAAAAAGLAIAPGIKLIEVAQARPEEQGASSAVRWGM
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHCCHHHHHHHHH
LVDATKCATGCDDCVSACSTENGWTPVAESAKPRQAPQWIRKLELQDPLTQMETNLPMMC
HHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHCCCHHHH
QHCAEPPCVDVCPTGASFKRADGIVLVNRHTCIGCRYCMMACPYKARSLVHEPLNDTQKA
HHCCCCCCCEECCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHC
DVPRGVGCVESCSLCVQKVDRGDGTTACAEACSAAGHNAIVFGDLNDPDSEISKRLRELP
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCC
TKQVRADLKLNTGVRYHGI
HHHHHHHHEECCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9389475; 11952791 [H]