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The map label for this gene is fis [C]

Identifier: 74318475

GI number: 74318475

Start: 2528181

End: 2528420

Strand: Direct

Name: fis [C]

Synonym: Tbd_2457

Alternate gene names: 74318475

Gene position: 2528181-2528420 (Clockwise)

Preceding gene: 74318474

Following gene: 74318476

Centisome position: 86.88

GC content: 60.42

Gene sequence:

>240_bases
ATGATAGAAGAAAACGAAATCGCCGCCTGCGTGCGGCGGTCGCTCAACCGCTATTTCAAGGACCTCGACGGCGAAATCCC
GAGCGAGATCTACGGCATGGTGGTGAGCGCGGTCGAAAAACCGCTCCTGGCCTACATCCTCGACCGCGCCGAGGGCAACC
AGACCCGCGCCGCCGAAATGCTCGGCATCAACCGCAATACGCTGCGCAAGAAAATGCGCGAACACGGCCTCTCTGACTGA

Upstream 100 bases:

>100_bases
CGCGCGGACGTCCGCCTCCGCTACGAAGCCGGCCAAGACAACAATAACGCGGACGACCTGCTGCCGGCCTCACGGCTCGC
GGCATAAGGGGAACTTCAAA

Downstream 100 bases:

>100_bases
CCCTTCCATGACGATCAAACGTGCCCTGCTTTCCGTGTCGGATAAAACCGGCCTCGTCGATTTCGCCCGCGCGCTCGCCA
CTGCCGGCGTCGAACTGCTG

Product: DNA-binding protein Fis

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 79; Mature: 79

Protein sequence:

>79_residues
MIEENEIAACVRRSLNRYFKDLDGEIPSEIYGMVVSAVEKPLLAYILDRAEGNQTRAAEMLGINRNTLRKKMREHGLSD

Sequences:

>Translated_79_residues
MIEENEIAACVRRSLNRYFKDLDGEIPSEIYGMVVSAVEKPLLAYILDRAEGNQTRAAEMLGINRNTLRKKMREHGLSD
>Mature_79_residues
MIEENEIAACVRRSLNRYFKDLDGEIPSEIYGMVVSAVEKPLLAYILDRAEGNQTRAAEMLGINRNTLRKKMREHGLSD

Specific function: Activates Ribosomal RNA Transcription. Plays A Direct Role In Upstream Activation Of rRNA Promoters. Binds To A Recombinational Enhancer Sequence That Is Required To Stimulate Hin-Mediated DNA Inversion. Prevents Initiation Of DNA Replication From Oric.

COG id: COG2901

COG function: function code KL; Factor for inversion stimulation Fis, transcriptional activator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transcriptional regulatory fis family [H]

Homologues:

Organism=Escherichia coli, GI1789661, Length=77, Percent_Identity=40.2597402597403, Blast_Score=73, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005412
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197 [H]

Pfam domain/function: PF02954 HTH_8 [H]

EC number: NA

Molecular weight: Translated: 9011; Mature: 9011

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEENEIAACVRRSLNRYFKDLDGEIPSEIYGMVVSAVEKPLLAYILDRAEGNQTRAAEM
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LGINRNTLRKKMREHGLSD
HCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIEENEIAACVRRSLNRYFKDLDGEIPSEIYGMVVSAVEKPLLAYILDRAEGNQTRAAEM
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LGINRNTLRKKMREHGLSD
HCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]