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The map label for this gene is purH [H]

Identifier: 74318476

GI number: 74318476

Start: 2528428

End: 2529987

Strand: Direct

Name: purH [H]

Synonym: Tbd_2458

Alternate gene names: 74318476

Gene position: 2528428-2529987 (Clockwise)

Preceding gene: 74318475

Following gene: 74318477

Centisome position: 86.89

GC content: 67.05

Gene sequence:

>1560_bases
ATGACGATCAAACGTGCCCTGCTTTCCGTGTCGGATAAAACCGGCCTCGTCGATTTCGCCCGCGCGCTCGCCACTGCCGG
CGTCGAACTGCTGTCCACGGGCGGCACCGCCAAGACCCTGCGCGACGCCGGCCTCGCGGTCATCGACGTCAGCGAATACA
CCGGCTTTCCCGAAATGCTCGACGGGCGCGTCAAGACCCTGCACCCGAAGGTGCACGGCGGCATCCTCGCGCGCCGCGAT
CTCGACGACCATGTCGCGACGATGGACACGCACAGCCTGGGCTACATCGACCTCGTGTGCGTGAATCTCTATCCCTTCGT
CGCCACGGTTTCCAAGCCGCATACCCTCGACGACGCGATCGAGAACATCGACATCGGCGGGCCGGCGATGGTGCGTTCGT
CGGCGAAAAACTACCGCTTCGTCGCGATCGTCACCGACCCGGCCGACTACCCGGCGCTCGTCGTCGAAATGCAGGCCAAC
GCCGGCGCGCTGACCGACGCGACCCGCTTCACGCTCGCCAAGAAGGCCTTCAGCCACACCGCGGAATACGACGGCGCGAT
CAGCAACTACCTGACGTCGCTCAACGACGCCAACGAGCGCGAGACCTTCGGCGAGAAACTGAACCTGCAGTTCACGCGCG
CGCAGATCTGCCGCTACGGCGAGAACCCGCATCAGGCCGGCGCCTTCTACGTGGAATCCGACGCGCCGGCCGGCACGATC
GCCACCGCGCGCCAGATCCAGGGCAAGGAGCTTTCCTACAACAACATTGCCGACACCGACGCCGCGCTCGAGTGCATCAA
GCAGTTCGACGCCGCGCCCGCCTGCGTCATCGTCAAGCACGCGAACCCGTGCGGCGTCGCGTACGGCACGAGCCTCATGG
AAGCCTACGACCGCGCCTACGCGACCGACCCCGAATCCGCGTTCGGCGGCATCATCGCCTTCAACGGCCGGCTCGACGGC
GCGACCGCCGCGGCGATCGTCGAACGCCAGTTCGTCGAGGTCATCATCGCCCCCGCAGTGAGCCCGGAAGCCTCGGCCGC
GGTCGCCGCCAAGAAGAACGTGCGCCTGCTCGAATGCGGCCAGTGGACAGGCAGCGTCGCCCAGCTCGACATGAAGCGCG
TCGCCGGCGGCCTCCTGGTGCAGGATGCCGACGTTTTGCTCAACGCCGAGCTCAAGGTCGTCACCCAACGCGCCCCAACC
GACAAGGAAATGGACGACCTCCTGTTCGCCTGGCGCGTCGCCAAGTTCGTCAAGTCGAACGCGATCGTCTACGCGAAGGA
CCGCATGACCATCGGCGTCGGCGCCGGCCAGATGAGCCGCGTCAACTCGGCGCGCATCGCCGCGATCAAGGCCGAGCACG
CCGGGCTCGTCGTGCCAGGCTCGGTCATGGCGTCGGACGCCTTCTTCCCTTTCCGCGACGGCATCGACCAGGCCGCGGCG
GCGGGCATCAAGGCCGTAATCCAGCCCGGCGGCTCGATGCGCGACGACGAGGTCATCGCCGCGGCGAACGAGCACGGTCT
CGCGATGGTGTTCACCGGCACGCGGCATTTCCGTCACTAG

Upstream 100 bases:

>100_bases
GCCGAGGGCAACCAGACCCGCGCCGCCGAAATGCTCGGCATCAACCGCAATACGCTGCGCAAGAAAATGCGCGAACACGG
CCTCTCTGACTGACCCTTCC

Downstream 100 bases:

>100_bases
GAATCAGAGGTCAGGTTTCAGGGATCAGGGAATGCGCCCGAGGCCGCCATTTTCCGAATCCACTCCACCTGACCTCTCCT
TGACCCCTGAATCCCGACCC

Product: IMP cyclohydrolase / phosphoribosylaminoimidazolecarboxamide formyltransferase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MTIKRALLSVSDKTGLVDFARALATAGVELLSTGGTAKTLRDAGLAVIDVSEYTGFPEMLDGRVKTLHPKVHGGILARRD
LDDHVATMDTHSLGYIDLVCVNLYPFVATVSKPHTLDDAIENIDIGGPAMVRSSAKNYRFVAIVTDPADYPALVVEMQAN
AGALTDATRFTLAKKAFSHTAEYDGAISNYLTSLNDANERETFGEKLNLQFTRAQICRYGENPHQAGAFYVESDAPAGTI
ATARQIQGKELSYNNIADTDAALECIKQFDAAPACVIVKHANPCGVAYGTSLMEAYDRAYATDPESAFGGIIAFNGRLDG
ATAAAIVERQFVEVIIAPAVSPEASAAVAAKKNVRLLECGQWTGSVAQLDMKRVAGGLLVQDADVLLNAELKVVTQRAPT
DKEMDDLLFAWRVAKFVKSNAIVYAKDRMTIGVGAGQMSRVNSARIAAIKAEHAGLVVPGSVMASDAFFPFRDGIDQAAA
AGIKAVIQPGGSMRDDEVIAAANEHGLAMVFTGTRHFRH

Sequences:

>Translated_519_residues
MTIKRALLSVSDKTGLVDFARALATAGVELLSTGGTAKTLRDAGLAVIDVSEYTGFPEMLDGRVKTLHPKVHGGILARRD
LDDHVATMDTHSLGYIDLVCVNLYPFVATVSKPHTLDDAIENIDIGGPAMVRSSAKNYRFVAIVTDPADYPALVVEMQAN
AGALTDATRFTLAKKAFSHTAEYDGAISNYLTSLNDANERETFGEKLNLQFTRAQICRYGENPHQAGAFYVESDAPAGTI
ATARQIQGKELSYNNIADTDAALECIKQFDAAPACVIVKHANPCGVAYGTSLMEAYDRAYATDPESAFGGIIAFNGRLDG
ATAAAIVERQFVEVIIAPAVSPEASAAVAAKKNVRLLECGQWTGSVAQLDMKRVAGGLLVQDADVLLNAELKVVTQRAPT
DKEMDDLLFAWRVAKFVKSNAIVYAKDRMTIGVGAGQMSRVNSARIAAIKAEHAGLVVPGSVMASDAFFPFRDGIDQAAA
AGIKAVIQPGGSMRDDEVIAAANEHGLAMVFTGTRHFRH
>Mature_518_residues
TIKRALLSVSDKTGLVDFARALATAGVELLSTGGTAKTLRDAGLAVIDVSEYTGFPEMLDGRVKTLHPKVHGGILARRDL
DDHVATMDTHSLGYIDLVCVNLYPFVATVSKPHTLDDAIENIDIGGPAMVRSSAKNYRFVAIVTDPADYPALVVEMQANA
GALTDATRFTLAKKAFSHTAEYDGAISNYLTSLNDANERETFGEKLNLQFTRAQICRYGENPHQAGAFYVESDAPAGTIA
TARQIQGKELSYNNIADTDAALECIKQFDAAPACVIVKHANPCGVAYGTSLMEAYDRAYATDPESAFGGIIAFNGRLDGA
TAAAIVERQFVEVIIAPAVSPEASAAVAAKKNVRLLECGQWTGSVAQLDMKRVAGGLLVQDADVLLNAELKVVTQRAPTD
KEMDDLLFAWRVAKFVKSNAIVYAKDRMTIGVGAGQMSRVNSARIAAIKAEHAGLVVPGSVMASDAFFPFRDGIDQAAAA
GIKAVIQPGGSMRDDEVIAAANEHGLAMVFTGTRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=511, Percent_Identity=37.7690802348337, Blast_Score=267, Evalue=2e-71,
Organism=Escherichia coli, GI1790439, Length=526, Percent_Identity=65.2091254752852, Blast_Score=671, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=519, Percent_Identity=34.4894026974952, Blast_Score=251, Evalue=8e-67,
Organism=Caenorhabditis elegans, GI71985574, Length=349, Percent_Identity=27.5071633237822, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71985556, Length=60, Percent_Identity=51.6666666666667, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323768, Length=481, Percent_Identity=37.006237006237, Blast_Score=256, Evalue=5e-69,
Organism=Saccharomyces cerevisiae, GI6323056, Length=486, Percent_Identity=36.4197530864198, Blast_Score=247, Evalue=4e-66,
Organism=Drosophila melanogaster, GI24649832, Length=514, Percent_Identity=37.9377431906615, Blast_Score=265, Evalue=8e-71,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55285; Mature: 55154

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKRALLSVSDKTGLVDFARALATAGVELLSTGGTAKTLRDAGLAVIDVSEYTGFPEML
CCHHHHHHHCCCCCCHHHHHHHHHHCCHHEEECCCCHHHHHHCCEEEEEEHHHCCCHHHH
DGRVKTLHPKVHGGILARRDLDDHVATMDTHSLGYIDLVCVNLYPFVATVSKPHTLDDAI
CCCCEEECCHHHCCEEEECCCCHHHHCCCCCCCCCCEEHHHHHHHHHHCCCCCCCHHHHH
ENIDIGGPAMVRSSAKNYRFVAIVTDPADYPALVVEMQANAGALTDATRFTLAKKAFSHT
HHCCCCCHHHHHCCCCCEEEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
AEYDGAISNYLTSLNDANERETFGEKLNLQFTRAQICRYGENPHQAGAFYVESDAPAGTI
HHCCHHHHHHHHHCCCCCHHHHHHHHCCEEEHHHHHHHCCCCCCCCCCEEEECCCCCCCH
ATARQIQGKELSYNNIADTDAALECIKQFDAAPACVIVKHANPCGVAYGTSLMEAYDRAY
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHC
ATDPESAFGGIIAFNGRLDGATAAAIVERQFVEVIIAPAVSPEASAAVAAKKNVRLLECG
CCCCCHHCCCEEEECCCCCCHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHCCCEEEECC
QWTGSVAQLDMKRVAGGLLVQDADVLLNAELKVVTQRAPTDKEMDDLLFAWRVAKFVKSN
CCCCHHHHHHHHHHHCCEEEECCCEEEECEEEEEEECCCCCCHHHHHHHHHHHHHHHHCC
AIVYAKDRMTIGVGAGQMSRVNSARIAAIKAEHAGLVVPGSVMASDAFFPFRDGIDQAAA
CEEEEECCEEEEECCCCHHHCCCCEEEEEEECCCCEEECCHHHHCCCCCCHHCCHHHHHH
AGIKAVIQPGGSMRDDEVIAAANEHGLAMVFTGTRHFRH
HCHHEEECCCCCCCCCCEEEEECCCCEEEEEECCHHCCC
>Mature Secondary Structure 
TIKRALLSVSDKTGLVDFARALATAGVELLSTGGTAKTLRDAGLAVIDVSEYTGFPEML
CHHHHHHHCCCCCCHHHHHHHHHHCCHHEEECCCCHHHHHHCCEEEEEEHHHCCCHHHH
DGRVKTLHPKVHGGILARRDLDDHVATMDTHSLGYIDLVCVNLYPFVATVSKPHTLDDAI
CCCCEEECCHHHCCEEEECCCCHHHHCCCCCCCCCCEEHHHHHHHHHHCCCCCCCHHHHH
ENIDIGGPAMVRSSAKNYRFVAIVTDPADYPALVVEMQANAGALTDATRFTLAKKAFSHT
HHCCCCCHHHHHCCCCCEEEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
AEYDGAISNYLTSLNDANERETFGEKLNLQFTRAQICRYGENPHQAGAFYVESDAPAGTI
HHCCHHHHHHHHHCCCCCHHHHHHHHCCEEEHHHHHHHCCCCCCCCCCEEEECCCCCCCH
ATARQIQGKELSYNNIADTDAALECIKQFDAAPACVIVKHANPCGVAYGTSLMEAYDRAY
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHC
ATDPESAFGGIIAFNGRLDGATAAAIVERQFVEVIIAPAVSPEASAAVAAKKNVRLLECG
CCCCCHHCCCEEEECCCCCCHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHCCCEEEECC
QWTGSVAQLDMKRVAGGLLVQDADVLLNAELKVVTQRAPTDKEMDDLLFAWRVAKFVKSN
CCCCHHHHHHHHHHHCCEEEECCCEEEECEEEEEEECCCCCCHHHHHHHHHHHHHHHHCC
AIVYAKDRMTIGVGAGQMSRVNSARIAAIKAEHAGLVVPGSVMASDAFFPFRDGIDQAAA
CEEEEECCEEEEECCCCHHHCCCCEEEEEEECCCCEEECCHHHHCCCCCCHHCCHHHHHH
AGIKAVIQPGGSMRDDEVIAAANEHGLAMVFTGTRHFRH
HCHHEEECCCCCCCCCCEEEEECCCCEEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA