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The map label for this gene is dusB [H]

Identifier: 74318474

GI number: 74318474

Start: 2527115

End: 2528167

Strand: Direct

Name: dusB [H]

Synonym: Tbd_2456

Alternate gene names: 74318474

Gene position: 2527115-2528167 (Clockwise)

Preceding gene: 74318471

Following gene: 74318475

Centisome position: 86.85

GC content: 68.57

Gene sequence:

>1053_bases
ATGCGCATCGGTTCCCACCTCCTGAAAAACCGCCTGATCGTCGCCCCGATGGCCGGCGTGACCGATCGGCCGTTTCGCCA
GCTGTGCAAGAAGCTCGGCGCGGGCATGGCGGTCTCGGAAATGGTGACGTCGAACTCGCTGCTGTACGGCTCGGCCAAGA
CGGCGCGGCGCGCCAACCACGAAGGCGAGGTCGACCCGATCAGCGTCCAGATCGCCGGCGCCGATCCGCGGATGATGGCC
GAGGCGGCGCGCCACAACGCCGACCGCGGCGCCCAGATCATCGACATCAACATGGGGTGCCCGGCGAAGAAGGTCTGCAA
CGTGATGGCCGGCTCGGCGCTGCTGCAGGACGAGGCGCTCGTCGGACGCATCCTCGACGCCGTCGTCGCGGCCGTGCCCG
AGCTGCCGGTGACGCTGAAGATCCGCACCGGCTGGGATCGCGAACACCGCAACGCACTCGCCATCCTCGGGATCGCCGAG
AACGCCGGCGTAAAGGCGCTCGCGATCCACGGCCGCACACGCGCCTGCGGCTACTCGGGCGAGGCCGAATACGACACGAT
CCGCGCGGTGAAGGCCGCGGCGCGGATTCCGGTCATCGCCAACGGTGACATCACGACCCCGGAAAAAGCGCGCCACGTGC
TCGAACACACGGGCGCCGACGCGGTCATGATCGGCCGCGCCGCCCAGGGGCGGCCCTGGATCTTCCGCGAGATCGAGCAT
TACCTGCGCACCGGCGAACACCTGCCGCAGCCTGAAGTGGCCGAAATCCACCGCGTGCTGCTCGATCACCTCCACGACCT
CTACGGCTTCTACGGCGAAGTCACCGGGGTGCGCGTCGCGCGCAAGCACATCTCCTGGTACACGCGCGGGCTCGTCGGCA
GCAGCGCTTTTCGCCACGCCATGAACCGGCTCGACTCGGTCGCCGAGCAGCTTGCCGTCGTCAACGACTATTTCGCCCAG
GCGGCGCGCGCGGACGTCCGCCTCCGCTACGAAGCCGGCCAAGACAACAATAACGCGGACGACCTGCTGCCGGCCTCACG
GCTCGCGGCATAA

Upstream 100 bases:

>100_bases
GATCCGGTCATAGTAGCCTGCCGGCCAACGGGCCGGGCGTACAGCGGTAATTTTTGCCGGCTTGGTGGGTCCCGGCGACG
GGAGTAAAATTCCAGCCCTT

Downstream 100 bases:

>100_bases
GGGGAACTTCAAAATGATAGAAGAAAACGAAATCGCCGCCTGCGTGCGGCGGTCGCTCAACCGCTATTTCAAGGACCTCG
ACGGCGAAATCCCGAGCGAG

Product: dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MRIGSHLLKNRLIVAPMAGVTDRPFRQLCKKLGAGMAVSEMVTSNSLLYGSAKTARRANHEGEVDPISVQIAGADPRMMA
EAARHNADRGAQIIDINMGCPAKKVCNVMAGSALLQDEALVGRILDAVVAAVPELPVTLKIRTGWDREHRNALAILGIAE
NAGVKALAIHGRTRACGYSGEAEYDTIRAVKAAARIPVIANGDITTPEKARHVLEHTGADAVMIGRAAQGRPWIFREIEH
YLRTGEHLPQPEVAEIHRVLLDHLHDLYGFYGEVTGVRVARKHISWYTRGLVGSSAFRHAMNRLDSVAEQLAVVNDYFAQ
AARADVRLRYEAGQDNNNADDLLPASRLAA

Sequences:

>Translated_350_residues
MRIGSHLLKNRLIVAPMAGVTDRPFRQLCKKLGAGMAVSEMVTSNSLLYGSAKTARRANHEGEVDPISVQIAGADPRMMA
EAARHNADRGAQIIDINMGCPAKKVCNVMAGSALLQDEALVGRILDAVVAAVPELPVTLKIRTGWDREHRNALAILGIAE
NAGVKALAIHGRTRACGYSGEAEYDTIRAVKAAARIPVIANGDITTPEKARHVLEHTGADAVMIGRAAQGRPWIFREIEH
YLRTGEHLPQPEVAEIHRVLLDHLHDLYGFYGEVTGVRVARKHISWYTRGLVGSSAFRHAMNRLDSVAEQLAVVNDYFAQ
AARADVRLRYEAGQDNNNADDLLPASRLAA
>Mature_350_residues
MRIGSHLLKNRLIVAPMAGVTDRPFRQLCKKLGAGMAVSEMVTSNSLLYGSAKTARRANHEGEVDPISVQIAGADPRMMA
EAARHNADRGAQIIDINMGCPAKKVCNVMAGSALLQDEALVGRILDAVVAAVPELPVTLKIRTGWDREHRNALAILGIAE
NAGVKALAIHGRTRACGYSGEAEYDTIRAVKAAARIPVIANGDITTPEKARHVLEHTGADAVMIGRAAQGRPWIFREIEH
YLRTGEHLPQPEVAEIHRVLLDHLHDLYGFYGEVTGVRVARKHISWYTRGLVGSSAFRHAMNRLDSVAEQLAVVNDYFAQ
AARADVRLRYEAGQDNNNADDLLPASRLAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=237, Percent_Identity=32.9113924050633, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI239788462, Length=237, Percent_Identity=32.9113924050633, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI31742496, Length=226, Percent_Identity=31.4159292035398, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI40807366, Length=231, Percent_Identity=29.8701298701299, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI8923374, Length=261, Percent_Identity=29.5019157088123, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI1789660, Length=322, Percent_Identity=59.0062111801242, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1788462, Length=252, Percent_Identity=32.5396825396825, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI145693211, Length=242, Percent_Identity=28.9256198347107, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25144369, Length=219, Percent_Identity=31.5068493150685, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17543114, Length=292, Percent_Identity=28.0821917808219, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17510279, Length=254, Percent_Identity=30.7086614173228, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17507177, Length=245, Percent_Identity=30.2040816326531, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323433, Length=258, Percent_Identity=31.3953488372093, Blast_Score=109, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6323560, Length=232, Percent_Identity=30.1724137931034, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=245, Percent_Identity=27.3469387755102, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24585320, Length=240, Percent_Identity=33.3333333333333, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24580595, Length=235, Percent_Identity=31.4893617021277, Blast_Score=98, Evalue=8e-21,
Organism=Drosophila melanogaster, GI19920448, Length=235, Percent_Identity=31.4893617021277, Blast_Score=98, Evalue=8e-21,
Organism=Drosophila melanogaster, GI45549423, Length=266, Percent_Identity=31.5789473684211, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19921524, Length=230, Percent_Identity=27.8260869565217, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 38045; Mature: 38045

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGSHLLKNRLIVAPMAGVTDRPFRQLCKKLGAGMAVSEMVTSNSLLYGSAKTARRANH
CCCHHHHHHCCEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHCCCEEECCCHHHHHCCC
EGEVDPISVQIAGADPRMMAEAARHNADRGAQIIDINMGCPAKKVCNVMAGSALLQDEAL
CCCCCEEEEEEECCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
VGRILDAVVAAVPELPVTLKIRTGWDREHRNALAILGIAENAGVKALAIHGRTRACGYSG
HHHHHHHHHHHCCCCCEEEEEECCCCHHHCCEEEEEEEECCCCEEEEEECCCCCCCCCCC
EAEYDTIRAVKAAARIPVIANGDITTPEKARHVLEHTGADAVMIGRAAQGRPWIFREIEH
CCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHH
YLRTGEHLPQPEVAEIHRVLLDHLHDLYGFYGEVTGVRVARKHISWYTRGLVGSSAFRHA
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNRLDSVAEQLAVVNDYFAQAARADVRLRYEAGQDNNNADDLLPASRLAA
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHCCCHHHHCC
>Mature Secondary Structure
MRIGSHLLKNRLIVAPMAGVTDRPFRQLCKKLGAGMAVSEMVTSNSLLYGSAKTARRANH
CCCHHHHHHCCEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHCCCEEECCCHHHHHCCC
EGEVDPISVQIAGADPRMMAEAARHNADRGAQIIDINMGCPAKKVCNVMAGSALLQDEAL
CCCCCEEEEEEECCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
VGRILDAVVAAVPELPVTLKIRTGWDREHRNALAILGIAENAGVKALAIHGRTRACGYSG
HHHHHHHHHHHCCCCCEEEEEECCCCHHHCCEEEEEEEECCCCEEEEEECCCCCCCCCCC
EAEYDTIRAVKAAARIPVIANGDITTPEKARHVLEHTGADAVMIGRAAQGRPWIFREIEH
CCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHH
YLRTGEHLPQPEVAEIHRVLLDHLHDLYGFYGEVTGVRVARKHISWYTRGLVGSSAFRHA
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNRLDSVAEQLAVVNDYFAQAARADVRLRYEAGQDNNNADDLLPASRLAA
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]