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The map label for this gene is bigR [H]

Identifier: 74318413

GI number: 74318413

Start: 2468373

End: 2468702

Strand: Direct

Name: bigR [H]

Synonym: Tbd_2395

Alternate gene names: 74318413

Gene position: 2468373-2468702 (Clockwise)

Preceding gene: 74318407

Following gene: 74318414

Centisome position: 84.83

GC content: 58.79

Gene sequence:

>330_bases
ATGAATATGGCAGCTGAGTGGGATGAAGTGGATCTCCTGGACAAGGACGAGCACATCGAGCAGGCCTCGCGTGCGATGAA
GGCCATGTCCCATCCGCTGCGGCTCAAGATTCTTTGCGTGCTCGGCGACAAGGAAGTCAGCGTCCAGGACATCGTCGACC
ACGTCGGTACCTCGCAAAGCAATATCTCGCAACACCTTGCGATCCTGCGCGACAAGGGCGTCCTGCGGACGCGCAAGGAC
GCCAACCGTGTGTTCTACCGCGTGGGCGATACGCGCACGCTCAAGCTGTTGGGCATGATGCGCGACGTGTTCTGCGGTTT
CACCAAATAA

Upstream 100 bases:

>100_bases
GCTAATCTATACGCGACCGCCAAAGTCGCGGGAAATGTGGCGCGCAAGCGCGTGGGGACAAGCCTCCAGACCGCGCCTGC
GGGACCCAAGGATCGAGGGA

Downstream 100 bases:

>100_bases
ACCCGGCATCTGCACCCTCTTCGACTTCTCCGCGTTTGCTCGCCCGGCACGCTGACCCGGCCTGAATAGCCAGCTCGACA
CCGTCATGCCGTCGGATACG

Product: ArsR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 109; Mature: 109

Protein sequence:

>109_residues
MNMAAEWDEVDLLDKDEHIEQASRAMKAMSHPLRLKILCVLGDKEVSVQDIVDHVGTSQSNISQHLAILRDKGVLRTRKD
ANRVFYRVGDTRTLKLLGMMRDVFCGFTK

Sequences:

>Translated_109_residues
MNMAAEWDEVDLLDKDEHIEQASRAMKAMSHPLRLKILCVLGDKEVSVQDIVDHVGTSQSNISQHLAILRDKGVLRTRKD
ANRVFYRVGDTRTLKLLGMMRDVFCGFTK
>Mature_109_residues
MNMAAEWDEVDLLDKDEHIEQASRAMKAMSHPLRLKILCVLGDKEVSVQDIVDHVGTSQSNISQHLAILRDKGVLRTRKD
ANRVFYRVGDTRTLKLLGMMRDVFCGFTK

Specific function: Represses an operon that comprises itself, XF_0764, XF_0765, XF_0766 and blh. Binds to a palindromic AT-rich sequence spanning the -10 region of the blh promoter and blocks transcription of the operon [H]

COG id: COG0640

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH arsR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789021, Length=87, Percent_Identity=39.0804597701149, Blast_Score=73, Evalue=5e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001845
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01022 HTH_5 [H]

EC number: NA

Molecular weight: Translated: 12429; Mature: 12429

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS50987 HTH_ARSR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMAAEWDEVDLLDKDEHIEQASRAMKAMSHPLRLKILCVLGDKEVSVQDIVDHVGTSQS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHCCCHH
NISQHLAILRDKGVLRTRKDANRVFYRVGDTRTLKLLGMMRDVFCGFTK
HHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNMAAEWDEVDLLDKDEHIEQASRAMKAMSHPLRLKILCVLGDKEVSVQDIVDHVGTSQS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHCCCHH
NISQHLAILRDKGVLRTRKDANRVFYRVGDTRTLKLLGMMRDVFCGFTK
HHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]