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The map label for this gene is slt [H]

Identifier: 74318380

GI number: 74318380

Start: 2435965

End: 2437929

Strand: Reverse

Name: slt [H]

Synonym: Tbd_2362

Alternate gene names: 74318380

Gene position: 2437929-2435965 (Counterclockwise)

Preceding gene: 74318381

Following gene: 74318379

Centisome position: 83.78

GC content: 69.16

Gene sequence:

>1965_bases
ATGCTTCGAACAGTCTTGGTTTTCCTGCTCGGCGTGCTGTCGGTCACGCCGGCCGCCGGCGCATCGGGTGACGCTGCGGT
AGTCGATGCGCAACGCGCGTATGCGGCGCGTCGGGTCGCGCAGCTCGAGCGCGCGGCTGCCGACGTCCCCCCCAGCCACG
TGCTCGCGCCGCTCGTCGAATACTGGCGCCTCGCGCTCGCCAGCCGCACCGACGACGCGCGGATTGCGGATTTCCTGGCG
CGCTATCCCGGCACGCGCATGGCTGAAGCATTGCGCGCGGACTGGCTCAAATCGCTCGGCGCACGCGGCGAGTGGGAGCG
CTTCCTCGCGGAGTATCCGCGCCTGGTCCGGGCCGATGCGTCGCTGCAGTGTTATGCGTACCGGGCCGAGCGGGCGCGCG
GAAACCTGACGCGTCAGGGCGAAGCCGTCGCCTTGTGGTTTACTGGCCGCGACATGCCATCGGCGTGCTCGCCGCTGTTC
GCGCAACTCATCGAGGCCGGCCTGATCGGCAGCGAAGACATCTGGCGGCGTTTTCGTCTCGCACTCGCCGCGCCCAACCC
GCGTGTCGCAGCTGTCGTCGCCCAGTCCGTAGCCGAGTCGCCGCAGCCTTCCGCGGCGTTGATCGACGCAGCCACCCGGG
CGCCGTCCGGGCTGCTCGCGAACCCGGGCAAGCTCGATTTCGGCCGCCGCGGCGATCGCGAGATCGCGCTCTACGCGCTC
GACCAGCTGGCGCGCCAGGATTCGAGCCAGGCGGAGCAGGCGCTGCGCCGCTGGTCGCCGCAGTTCACCGACAGCGAGCG
ACGGGTGGCGTGGGCGCGCCTCGCGACCTGGGCGGCGCGGCGTCACGAGGCGAATGCATTGACCTGGTTCGCCGCCGCCG
GGGAAGTCGAGACGACCGATTTCCAGCGCGAATGGTGGGTGCGTGCAGCACTCCGTGCGGGCGACTGGAAAACCGTCGAA
CGGGTGATCGACAGCATGAGCGAGGACCTGCGCGCGCAGGCGACATGGCGCTACTGGCGCGGCCGCGCGCTGCAGGCCAA
TGGTTCGCGCGGCGCGGCGAACGCCATTTTTCTGGCCCTGTCGCGCGAGCACCATTACTACGGGCAGCTCGCGCAGGAGG
AACTCGGCCCGGTGCTGCAGTCGCCGTTGCCCAACGTGAAGATGGGCGGCGACGACCTCGCTGCGATGGCGCGCGACCCC
GGTATCGCACGGGCGCTCGCGCTGCTCGACCTCGGCTTGCGCAACGAGGCGACGCTCGAGTGGAACTGGGCGACGCGTGA
ATTTACCGACGCCCAGCTCCTCGCCGCCGCGGAAGTGGCGCGGCGCAAGGAGTGGTACGACCGCGCGATCTACACCGCGG
AGCGCACGCGCGACATGCACGATTTCGAGTTGCGCTTTCTCGCGCCGTACCGCGAGCTCGCGCGCGAGGCGGCGCGCGAA
AACGGCATCGACGAAGCCTGGGTGTTCGGCTTGATGCGGCAGGAAAGCCGCTTCGTCAACGTGGCACGTTCGAGCGTGGG
TGCCGCGGGCCTGATGCAGATCATGCCGGCGACGGCACGCTGGATCGCGCAACGGCTCGGCATCCGCAAATTCGACCCGC
GTGAGATGCGCGACCCGGCACGCAATATCCAGTTCGGGGCGTATTACCTCAAGCACGTGCAGACGTCGCTGGACGGCTCG
CCGGTGCTTGCCACGGCCGCCTACAACGCCGGCCCCGGGCGCGCGCAACGCTGGCGCGACACGGCGGCGATGGAAGCCGC
GGTCTACATCGAGTCGATCCCCTTCGCCGAGACGCGCGATTACGTCAAGAAGGTGATGAGCAACGCGATGTACTACGCGG
TGCGCTTTGGCCAGCCGTCGGTGTTGTTGAAAGACCGGCTCGGCGTCGTGCCGCCGCGCAGGATTCCGCTCGCGGCCCTC
ACGGAACCACAAGGCTCTCTGGAACTCGGTCAGACGGACGATTAA

Upstream 100 bases:

>100_bases
CTTGCGGCGCGGGTGAACGGCCCCGGGCGCGGCGCCGCCGACGTCATCGTAGTGAATTCCGCTCAGGTAGGGCGGCCGGG
GCGCATGTTACCCTCGTGCG

Downstream 100 bases:

>100_bases
AATCGGCGCTGCCTTCTATCCCTATATTCCTGCACACGATTCCATGAAGATTCAGCGCATTTGCGTTCTCGGCGGCTCCG
GCTTCGTCGGTACCCACCTC

Product: SLT domain-containing protein

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 654; Mature: 654

Protein sequence:

>654_residues
MLRTVLVFLLGVLSVTPAAGASGDAAVVDAQRAYAARRVAQLERAAADVPPSHVLAPLVEYWRLALASRTDDARIADFLA
RYPGTRMAEALRADWLKSLGARGEWERFLAEYPRLVRADASLQCYAYRAERARGNLTRQGEAVALWFTGRDMPSACSPLF
AQLIEAGLIGSEDIWRRFRLALAAPNPRVAAVVAQSVAESPQPSAALIDAATRAPSGLLANPGKLDFGRRGDREIALYAL
DQLARQDSSQAEQALRRWSPQFTDSERRVAWARLATWAARRHEANALTWFAAAGEVETTDFQREWWVRAALRAGDWKTVE
RVIDSMSEDLRAQATWRYWRGRALQANGSRGAANAIFLALSREHHYYGQLAQEELGPVLQSPLPNVKMGGDDLAAMARDP
GIARALALLDLGLRNEATLEWNWATREFTDAQLLAAAEVARRKEWYDRAIYTAERTRDMHDFELRFLAPYRELAREAARE
NGIDEAWVFGLMRQESRFVNVARSSVGAAGLMQIMPATARWIAQRLGIRKFDPREMRDPARNIQFGAYYLKHVQTSLDGS
PVLATAAYNAGPGRAQRWRDTAAMEAAVYIESIPFAETRDYVKKVMSNAMYYAVRFGQPSVLLKDRLGVVPPRRIPLAAL
TEPQGSLELGQTDD

Sequences:

>Translated_654_residues
MLRTVLVFLLGVLSVTPAAGASGDAAVVDAQRAYAARRVAQLERAAADVPPSHVLAPLVEYWRLALASRTDDARIADFLA
RYPGTRMAEALRADWLKSLGARGEWERFLAEYPRLVRADASLQCYAYRAERARGNLTRQGEAVALWFTGRDMPSACSPLF
AQLIEAGLIGSEDIWRRFRLALAAPNPRVAAVVAQSVAESPQPSAALIDAATRAPSGLLANPGKLDFGRRGDREIALYAL
DQLARQDSSQAEQALRRWSPQFTDSERRVAWARLATWAARRHEANALTWFAAAGEVETTDFQREWWVRAALRAGDWKTVE
RVIDSMSEDLRAQATWRYWRGRALQANGSRGAANAIFLALSREHHYYGQLAQEELGPVLQSPLPNVKMGGDDLAAMARDP
GIARALALLDLGLRNEATLEWNWATREFTDAQLLAAAEVARRKEWYDRAIYTAERTRDMHDFELRFLAPYRELAREAARE
NGIDEAWVFGLMRQESRFVNVARSSVGAAGLMQIMPATARWIAQRLGIRKFDPREMRDPARNIQFGAYYLKHVQTSLDGS
PVLATAAYNAGPGRAQRWRDTAAMEAAVYIESIPFAETRDYVKKVMSNAMYYAVRFGQPSVLLKDRLGVVPPRRIPLAAL
TEPQGSLELGQTDD
>Mature_654_residues
MLRTVLVFLLGVLSVTPAAGASGDAAVVDAQRAYAARRVAQLERAAADVPPSHVLAPLVEYWRLALASRTDDARIADFLA
RYPGTRMAEALRADWLKSLGARGEWERFLAEYPRLVRADASLQCYAYRAERARGNLTRQGEAVALWFTGRDMPSACSPLF
AQLIEAGLIGSEDIWRRFRLALAAPNPRVAAVVAQSVAESPQPSAALIDAATRAPSGLLANPGKLDFGRRGDREIALYAL
DQLARQDSSQAEQALRRWSPQFTDSERRVAWARLATWAARRHEANALTWFAAAGEVETTDFQREWWVRAALRAGDWKTVE
RVIDSMSEDLRAQATWRYWRGRALQANGSRGAANAIFLALSREHHYYGQLAQEELGPVLQSPLPNVKMGGDDLAAMARDP
GIARALALLDLGLRNEATLEWNWATREFTDAQLLAAAEVARRKEWYDRAIYTAERTRDMHDFELRFLAPYRELAREAARE
NGIDEAWVFGLMRQESRFVNVARSSVGAAGLMQIMPATARWIAQRLGIRKFDPREMRDPARNIQFGAYYLKHVQTSLDGS
PVLATAAYNAGPGRAQRWRDTAAMEAAVYIESIPFAETRDYVKKVMSNAMYYAVRFGQPSVLLKDRLGVVPPRRIPLAAL
TEPQGSLELGQTDD

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=641, Percent_Identity=27.7691107644306, Blast_Score=186, Evalue=4e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 72913; Mature: 72913

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRTVLVFLLGVLSVTPAAGASGDAAVVDAQRAYAARRVAQLERAAADVPPSHVLAPLVE
CHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
YWRLALASRTDDARIADFLARYPGTRMAEALRADWLKSLGARGEWERFLAEYPRLVRADA
HHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHCCC
SLQCYAYRAERARGNLTRQGEAVALWFTGRDMPSACSPLFAQLIEAGLIGSEDIWRRFRL
CCEEEEEHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
ALAAPNPRVAAVVAQSVAESPQPSAALIDAATRAPSGLLANPGKLDFGRRGDREIALYAL
HHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHH
DQLARQDSSQAEQALRRWSPQFTDSERRVAWARLATWAARRHEANALTWFAAAGEVETTD
HHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCH
FQREWWVRAALRAGDWKTVERVIDSMSEDLRAQATWRYWRGRALQANGSRGAANAIFLAL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEE
SREHHYYGQLAQEELGPVLQSPLPNVKMGGDDLAAMARDPGIARALALLDLGLRNEATLE
ECCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCEEE
WNWATREFTDAQLLAAAEVARRKEWYDRAIYTAERTRDMHDFELRFLAPYRELAREAARE
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEHHHHHHHHHHHHH
NGIDEAWVFGLMRQESRFVNVARSSVGAAGLMQIMPATARWIAQRLGIRKFDPREMRDPA
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHH
RNIQFGAYYLKHVQTSLDGSPVLATAAYNAGPGRAQRWRDTAAMEAAVYIESIPFAETRD
HCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
YVKKVMSNAMYYAVRFGQPSVLLKDRLGVVPPRRIPLAALTEPQGSLELGQTDD
HHHHHHCCCEEEEEECCCCCEEHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCC
>Mature Secondary Structure
MLRTVLVFLLGVLSVTPAAGASGDAAVVDAQRAYAARRVAQLERAAADVPPSHVLAPLVE
CHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
YWRLALASRTDDARIADFLARYPGTRMAEALRADWLKSLGARGEWERFLAEYPRLVRADA
HHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHCCC
SLQCYAYRAERARGNLTRQGEAVALWFTGRDMPSACSPLFAQLIEAGLIGSEDIWRRFRL
CCEEEEEHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
ALAAPNPRVAAVVAQSVAESPQPSAALIDAATRAPSGLLANPGKLDFGRRGDREIALYAL
HHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHH
DQLARQDSSQAEQALRRWSPQFTDSERRVAWARLATWAARRHEANALTWFAAAGEVETTD
HHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCH
FQREWWVRAALRAGDWKTVERVIDSMSEDLRAQATWRYWRGRALQANGSRGAANAIFLAL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEE
SREHHYYGQLAQEELGPVLQSPLPNVKMGGDDLAAMARDPGIARALALLDLGLRNEATLE
ECCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCEEE
WNWATREFTDAQLLAAAEVARRKEWYDRAIYTAERTRDMHDFELRFLAPYRELAREAARE
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEHHHHHHHHHHHHH
NGIDEAWVFGLMRQESRFVNVARSSVGAAGLMQIMPATARWIAQRLGIRKFDPREMRDPA
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHH
RNIQFGAYYLKHVQTSLDGSPVLATAAYNAGPGRAQRWRDTAAMEAAVYIESIPFAETRD
HCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
YVKKVMSNAMYYAVRFGQPSVLLKDRLGVVPPRRIPLAALTEPQGSLELGQTDD
HHHHHHCCCEEEEEECCCCCEEHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]