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The map label for this gene is ilvA [H]

Identifier: 74318381

GI number: 74318381

Start: 2438064

End: 2439581

Strand: Reverse

Name: ilvA [H]

Synonym: Tbd_2363

Alternate gene names: 74318381

Gene position: 2439581-2438064 (Counterclockwise)

Preceding gene: 74318383

Following gene: 74318380

Centisome position: 83.84

GC content: 65.61

Gene sequence:

>1518_bases
ATGAGCCACCCAGACGACTACCTCGAACGCATTCTCACCTCGCGCGTCTATGACGTCGCGATCGAATCGCCGCTCGAGGT
CGCGCACAATCTTTCGCGTCGCCTCGACAACCAGATCCTGCTCAAGCGCGAGGACCTGCAGCCGGTGTTCTCGTTCAAGT
GCCGCGGCGCGTACAACAAGATGGCGAAGTTGACCGCCGCGCAACTGGCGCGCGGCGTCGTCGCGGCGTCCGCCGGCAAC
CACGCGCAGGGCGTCGCTTTGGCGGCCGAAAAGCTCGGCGCGACGGCCACCATCGTCATGCCGGCGACGACCCCGAGGAT
CAAGGTCGACGCGGTCGCGGCGCGCGGCGCCCGCGTGATCCTGCACGGCGACAACTACGACGAAGCCTACGAGCATGCGA
TGCAGCTGGCGAAGGAGGCGAAGGCGACCTTCGTCCATCCCTACGACGATCCCGACGTGATCGCGGGCCAGGGCACGGTC
GCGATGGAGTTGCTGCGCCAGCACCCGGGCCCGATCCACTCCGTCTACATCCCGGTCGGGGGCGGCGGTCTGATCGCGGG
TATGGCCGCCTACATCAAGCGGCTGCGGCCGCAGATCAAGATCGTCGGCGTCGAGCCCGAGGACGCCGACGCGATGGCGC
GTTCGCTGTGCAAGAACACGCGCGTGACGCTGCCGCGCGTCGGCATTTTTGCCGACGGCGTCGCGGTAAAAAAGGTCGGC
AAGGAAACCTTCCGGCTGGCGCAGCTCTATGTCGACGAAATCATCCGCGTGAACAACGACGCGATCTGCGCAGCGATCAA
GGACGTCTTCGAGGACACCCGTTCGATCCTCGAACCCGCGGGCGCGCTTTCGATCGCCGGCATGAAAGCGGCGATCGCCG
GTGACAGGCTGAAGGGGAAGACGCTTGCCGCCGTGGCGTCTGGCGCCAACATGAACTTCGACCGCCTGCGCCATATTGCG
GAGCGCGCGGAACTGGGCGAGAAGCGCGAAGCGATTCTCGCGGTGACCATTCCCGAAACGCCGGGCAGCTTCAAGACCTT
CTGCGGCCTGCTCGGCGCGCGCAACATCACCGAATTCAACTATCGCTACGCCGATCCGCGTGTGGCGCGGGTGTTCGTCG
GACTGTCCGTACCCGGTGAGGGCGAGAGCAAGCGCGTCGTCGAACTGCTCGGGCGGCACGGCCTCGAAGCGATCGATCTG
AGCGACAACGAAATGGCCAAGCTCCACATCCGCCACCTGGTCGGCGGGCGCGCGCCCGACGCGGTGAACGAGGTGCTGTA
CCGCTTCGAGTTTCCCGAGCGCCCGGGCGCGCTGATGCAGTTCCTGCAAAGCATGAGCCGCGGCTGGAACATCAGCCTCT
TTCATTATCGCAACCACGGCACCGACTACGGGCGCGTGCTGGTCGGCATCCAGGTGCCGGACAAGGAAAAACCGGTCTTT
CAGAAATTCCTCGAAGGCCTCGGCTACCGCTACTGGGACGAGTCGCGCAACCCGGCGTACACGCTCTTCCTGGGCTGA

Upstream 100 bases:

>100_bases
TTCATTTCGTCTTGGGTCATGATGTTTTCGTCGATTTGAGTCCAGCCCACTAAGATAGCGGCATCGCACCCTTTTTACAA
ACGCACCTCAGCCGCCCGCC

Downstream 100 bases:

>100_bases
GCTACGATCGCTCCTACCGTGCTGGGGCAGCGGGCTTGCGGCGCGGGTGAACGGCCCCGGGCGCGGCGCCGCCGACGTCA
TCGTAGTGAATTCCGCTCAG

Product: L-threonine ammonia-lyase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MSHPDDYLERILTSRVYDVAIESPLEVAHNLSRRLDNQILLKREDLQPVFSFKCRGAYNKMAKLTAAQLARGVVAASAGN
HAQGVALAAEKLGATATIVMPATTPRIKVDAVAARGARVILHGDNYDEAYEHAMQLAKEAKATFVHPYDDPDVIAGQGTV
AMELLRQHPGPIHSVYIPVGGGGLIAGMAAYIKRLRPQIKIVGVEPEDADAMARSLCKNTRVTLPRVGIFADGVAVKKVG
KETFRLAQLYVDEIIRVNNDAICAAIKDVFEDTRSILEPAGALSIAGMKAAIAGDRLKGKTLAAVASGANMNFDRLRHIA
ERAELGEKREAILAVTIPETPGSFKTFCGLLGARNITEFNYRYADPRVARVFVGLSVPGEGESKRVVELLGRHGLEAIDL
SDNEMAKLHIRHLVGGRAPDAVNEVLYRFEFPERPGALMQFLQSMSRGWNISLFHYRNHGTDYGRVLVGIQVPDKEKPVF
QKFLEGLGYRYWDESRNPAYTLFLG

Sequences:

>Translated_505_residues
MSHPDDYLERILTSRVYDVAIESPLEVAHNLSRRLDNQILLKREDLQPVFSFKCRGAYNKMAKLTAAQLARGVVAASAGN
HAQGVALAAEKLGATATIVMPATTPRIKVDAVAARGARVILHGDNYDEAYEHAMQLAKEAKATFVHPYDDPDVIAGQGTV
AMELLRQHPGPIHSVYIPVGGGGLIAGMAAYIKRLRPQIKIVGVEPEDADAMARSLCKNTRVTLPRVGIFADGVAVKKVG
KETFRLAQLYVDEIIRVNNDAICAAIKDVFEDTRSILEPAGALSIAGMKAAIAGDRLKGKTLAAVASGANMNFDRLRHIA
ERAELGEKREAILAVTIPETPGSFKTFCGLLGARNITEFNYRYADPRVARVFVGLSVPGEGESKRVVELLGRHGLEAIDL
SDNEMAKLHIRHLVGGRAPDAVNEVLYRFEFPERPGALMQFLQSMSRGWNISLFHYRNHGTDYGRVLVGIQVPDKEKPVF
QKFLEGLGYRYWDESRNPAYTLFLG
>Mature_504_residues
SHPDDYLERILTSRVYDVAIESPLEVAHNLSRRLDNQILLKREDLQPVFSFKCRGAYNKMAKLTAAQLARGVVAASAGNH
AQGVALAAEKLGATATIVMPATTPRIKVDAVAARGARVILHGDNYDEAYEHAMQLAKEAKATFVHPYDDPDVIAGQGTVA
MELLRQHPGPIHSVYIPVGGGGLIAGMAAYIKRLRPQIKIVGVEPEDADAMARSLCKNTRVTLPRVGIFADGVAVKKVGK
ETFRLAQLYVDEIIRVNNDAICAAIKDVFEDTRSILEPAGALSIAGMKAAIAGDRLKGKTLAAVASGANMNFDRLRHIAE
RAELGEKREAILAVTIPETPGSFKTFCGLLGARNITEFNYRYADPRVARVFVGLSVPGEGESKRVVELLGRHGLEAIDLS
DNEMAKLHIRHLVGGRAPDAVNEVLYRFEFPERPGALMQFLQSMSRGWNISLFHYRNHGTDYGRVLVGIQVPDKEKPVFQ
KFLEGLGYRYWDESRNPAYTLFLG

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=308, Percent_Identity=33.4415584415584, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI33469958, Length=329, Percent_Identity=27.6595744680851, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI19923959, Length=317, Percent_Identity=27.1293375394322, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1790207, Length=500, Percent_Identity=55.4, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI1789505, Length=318, Percent_Identity=35.8490566037736, Blast_Score=174, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI71991565, Length=345, Percent_Identity=33.3333333333333, Blast_Score=155, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17537387, Length=340, Percent_Identity=33.5294117647059, Blast_Score=151, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17508781, Length=264, Percent_Identity=31.4393939393939, Blast_Score=128, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6320930, Length=515, Percent_Identity=53.7864077669903, Blast_Score=512, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6322631, Length=303, Percent_Identity=36.3036303630363, Blast_Score=170, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6319788, Length=295, Percent_Identity=29.4915254237288, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21355833, Length=314, Percent_Identity=32.8025477707006, Blast_Score=152, Evalue=6e-37,
Organism=Drosophila melanogaster, GI24645328, Length=252, Percent_Identity=30.5555555555556, Blast_Score=110, Evalue=3e-24,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR005787
- InterPro:   IPR001721 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 55363; Mature: 55232

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHPDDYLERILTSRVYDVAIESPLEVAHNLSRRLDNQILLKREDLQPVFSFKCRGAYNK
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEHHCCCHHHHEECCCHHHH
MAKLTAAQLARGVVAASAGNHAQGVALAAEKLGATATIVMPATTPRIKVDAVAARGARVI
HHHHHHHHHHHHHHEECCCCCCCHHHHHHHHCCCEEEEEEECCCCCEEEEEEECCCCEEE
LHGDNYDEAYEHAMQLAKEAKATFVHPYDDPDVIAGQGTVAMELLRQHPGPIHSVYIPVG
EECCCHHHHHHHHHHHHHHHHCEEECCCCCCCEEECCCHHHHHHHHHCCCCCEEEEEEEC
GGGLIAGMAAYIKRLRPQIKIVGVEPEDADAMARSLCKNTRVTLPRVGIFADGVAVKKVG
CCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHH
KETFRLAQLYVDEIIRVNNDAICAAIKDVFEDTRSILEPAGALSIAGMKAAIAGDRLKGK
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHCCCCCCCC
TLAAVASGANMNFDRLRHIAERAELGEKREAILAVTIPETPGSFKTFCGLLGARNITEFN
EEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCCC
YRYADPRVARVFVGLSVPGEGESKRVVELLGRHGLEAIDLSDNEMAKLHIRHLVGGRAPD
EEECCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCCCH
AVNEVLYRFEFPERPGALMQFLQSMSRGWNISLFHYRNHGTDYGRVLVGIQVPDKEKPVF
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEEECCCCCCHHH
QKFLEGLGYRYWDESRNPAYTLFLG
HHHHHHCCCEECCCCCCCEEEEEEC
>Mature Secondary Structure 
SHPDDYLERILTSRVYDVAIESPLEVAHNLSRRLDNQILLKREDLQPVFSFKCRGAYNK
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEHHCCCHHHHEECCCHHHH
MAKLTAAQLARGVVAASAGNHAQGVALAAEKLGATATIVMPATTPRIKVDAVAARGARVI
HHHHHHHHHHHHHHEECCCCCCCHHHHHHHHCCCEEEEEEECCCCCEEEEEEECCCCEEE
LHGDNYDEAYEHAMQLAKEAKATFVHPYDDPDVIAGQGTVAMELLRQHPGPIHSVYIPVG
EECCCHHHHHHHHHHHHHHHHCEEECCCCCCCEEECCCHHHHHHHHHCCCCCEEEEEEEC
GGGLIAGMAAYIKRLRPQIKIVGVEPEDADAMARSLCKNTRVTLPRVGIFADGVAVKKVG
CCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHH
KETFRLAQLYVDEIIRVNNDAICAAIKDVFEDTRSILEPAGALSIAGMKAAIAGDRLKGK
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHCCCCCCCC
TLAAVASGANMNFDRLRHIAERAELGEKREAILAVTIPETPGSFKTFCGLLGARNITEFN
EEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCCC
YRYADPRVARVFVGLSVPGEGESKRVVELLGRHGLEAIDLSDNEMAKLHIRHLVGGRAPD
EEECCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCCCH
AVNEVLYRFEFPERPGALMQFLQSMSRGWNISLFHYRNHGTDYGRVLVGIQVPDKEKPVF
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEEECCCCCCHHH
QKFLEGLGYRYWDESRNPAYTLFLG
HHHHHHCCCEECCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA