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The map label for this gene is hmp [H]

Identifier: 74318347

GI number: 74318347

Start: 2402908

End: 2404095

Strand: Direct

Name: hmp [H]

Synonym: Tbd_2329

Alternate gene names: 74318347

Gene position: 2402908-2404095 (Clockwise)

Preceding gene: 74318346

Following gene: 74318355

Centisome position: 82.58

GC content: 66.33

Gene sequence:

>1188_bases
ATGCTATCTGCCGAAACCCTGGCAACGATCAAGTCCACCGCACCGCTCCTCGCCGAGCAGGGCCGTGCAATCACCGATCT
GTTTTATTCCAAGCTCTTTTCCGAACACCCGGAACTGCAGCACATCTTCAACATGGCAAACCAGGCGCAGGGCGAGCAGT
CGCGCGCGCTCGCAGAATCCGTCTTCATGTATGCGACGCACATCGACGCGCTGCAGAAACTCGGCCCGATGGTCAGCCGC
ATCGCCCACAAGCACGCGAGCCTGCAGGTCGCCCCCGAACACTATCCGATCGTCGGCAAATATCTGCTCGAGGCGATCCG
CGACCACCTCGGGCTGGAAGACGGCCATCCGGTGCTTGGCGCCTGGTCCGAGGCCTACGCGCAACTCGCCGATATCTTCA
TCACGACCGAAGAGGGCATCTACGCGGCAAACGCCGACAAGCCCGGCGGCTGGCGCGGCTTCCGCGCCTTCGTGATCGAC
GAGATCCGCGACGAGGCGCAGGGCATCAAGTCGATCTATCTGGTTCCCGAGGACGGTCAGGCGATCGCCGACTTCGAGCC
GGGGCAGTACCTCGGCGTCAAGGTTCAGGTGCCCGGCCACGCGTACGACGAGATCCGCCAGTATTCGCTGTCCGACGCAC
CGGGCAAGCGCACCTACCGCATCACGGTCAAGGCCGAGGGGGACGCGCCTGCGGGCAAGGTGTCCCACCATCTGCACGGC
GCCGAGCCCGGCGACCGGGTCTGGGTACAGCCGCCGACCGGCGACTTCACGGTCGAGCGTGAAGACCGCCGGCTCGCGTT
CATCGCCGGAGGCGTCGGCATCACACCGCTGCTCAGCATGCTGCACGCGCGTGCCGAGCGCGGCGCCGACCTCTCCGACG
TCGTCTTCGTGCACTGCTGCCGCGACAAGGCGCACCATGCGATGGCGGACGAGTTGCGGCAGCTTGCCCGCGCGCACGGC
TTCTCCTACCGCGTCGCCTACGAGCGCGGCGAGGGCGGTGATCATCAAGGCTATCTCGATCGCGACGTGCTCACGCGCTG
GCTCGGCGAGCCCGACGCCGACGTCTACTTCTGCGGGCCTAGGCCCTTCATGGCCGCGCTCAATACCGCGCTCGGCGAGA
TGGGCTACCGCGAGGAACGACTGCACTACGAGGTGTTCGGCCCGAGCATCCGCCTGCAGCCGCATTGA

Upstream 100 bases:

>100_bases
CAGTATCTTGCTTGCCCCGAAAATTAGATTCACCTACTATGCGCGTCCATTTCATGCATACGGTATGCATGTTTTGTCCG
AAACGCCTCAAGGAGAAACG

Downstream 100 bases:

>100_bases
GAAAAGGGAGCCTCGGGCTCCCTTTTTTTCTAGGCTTTCCGCGCCACGAGGTCGATCAAGGCAGACTGACCATGATGATG
CGAGCCTTCGCTGATGAAGG

Product: flavohemoglobin

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MLSAETLATIKSTAPLLAEQGRAITDLFYSKLFSEHPELQHIFNMANQAQGEQSRALAESVFMYATHIDALQKLGPMVSR
IAHKHASLQVAPEHYPIVGKYLLEAIRDHLGLEDGHPVLGAWSEAYAQLADIFITTEEGIYAANADKPGGWRGFRAFVID
EIRDEAQGIKSIYLVPEDGQAIADFEPGQYLGVKVQVPGHAYDEIRQYSLSDAPGKRTYRITVKAEGDAPAGKVSHHLHG
AEPGDRVWVQPPTGDFTVEREDRRLAFIAGGVGITPLLSMLHARAERGADLSDVVFVHCCRDKAHHAMADELRQLARAHG
FSYRVAYERGEGGDHQGYLDRDVLTRWLGEPDADVYFCGPRPFMAALNTALGEMGYREERLHYEVFGPSIRLQPH

Sequences:

>Translated_395_residues
MLSAETLATIKSTAPLLAEQGRAITDLFYSKLFSEHPELQHIFNMANQAQGEQSRALAESVFMYATHIDALQKLGPMVSR
IAHKHASLQVAPEHYPIVGKYLLEAIRDHLGLEDGHPVLGAWSEAYAQLADIFITTEEGIYAANADKPGGWRGFRAFVID
EIRDEAQGIKSIYLVPEDGQAIADFEPGQYLGVKVQVPGHAYDEIRQYSLSDAPGKRTYRITVKAEGDAPAGKVSHHLHG
AEPGDRVWVQPPTGDFTVEREDRRLAFIAGGVGITPLLSMLHARAERGADLSDVVFVHCCRDKAHHAMADELRQLARAHG
FSYRVAYERGEGGDHQGYLDRDVLTRWLGEPDADVYFCGPRPFMAALNTALGEMGYREERLHYEVFGPSIRLQPH
>Mature_395_residues
MLSAETLATIKSTAPLLAEQGRAITDLFYSKLFSEHPELQHIFNMANQAQGEQSRALAESVFMYATHIDALQKLGPMVSR
IAHKHASLQVAPEHYPIVGKYLLEAIRDHLGLEDGHPVLGAWSEAYAQLADIFITTEEGIYAANADKPGGWRGFRAFVID
EIRDEAQGIKSIYLVPEDGQAIADFEPGQYLGVKVQVPGHAYDEIRQYSLSDAPGKRTYRITVKAEGDAPAGKVSHHLHG
AEPGDRVWVQPPTGDFTVEREDRRLAFIAGGVGITPLLSMLHARAERGADLSDVVFVHCCRDKAHHAMADELRQLARAHG
FSYRVAYERGEGGDHQGYLDRDVLTRWLGEPDADVYFCGPRPFMAALNTALGEMGYREERLHYEVFGPSIRLQPH

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=399, Percent_Identity=41.3533834586466, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1788104, Length=233, Percent_Identity=28.3261802575107, Blast_Score=75, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6321673, Length=408, Percent_Identity=36.0294117647059, Blast_Score=217, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6323510, Length=175, Percent_Identity=30.2857142857143, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322146, Length=204, Percent_Identity=25.4901960784314, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR001834
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 43821; Mature: 43821

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSAETLATIKSTAPLLAEQGRAITDLFYSKLFSEHPELQHIFNMANQAQGEQSRALAES
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHH
VFMYATHIDALQKLGPMVSRIAHKHASLQVAPEHYPIVGKYLLEAIRDHLGLEDGHPVLG
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
AWSEAYAQLADIFITTEEGIYAANADKPGGWRGFRAFVIDEIRDEAQGIKSIYLVPEDGQ
CHHHHHHHHHHEEEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCC
AIADFEPGQYLGVKVQVPGHAYDEIRQYSLSDAPGKRTYRITVKAEGDAPAGKVSHHLHG
EEECCCCCCEEEEEEEECCCHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCHHHHCCCC
AEPGDRVWVQPPTGDFTVEREDRRLAFIAGGVGITPLLSMLHARAERGADLSDVVFVHCC
CCCCCEEEEECCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
RDKAHHAMADELRQLARAHGFSYRVAYERGEGGDHQGYLDRDVLTRWLGEPDADVYFCGP
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCEEEECC
RPFMAALNTALGEMGYREERLHYEVFGPSIRLQPH
CHHHHHHHHHHHHCCCHHHHCEEEEECCEEEECCC
>Mature Secondary Structure
MLSAETLATIKSTAPLLAEQGRAITDLFYSKLFSEHPELQHIFNMANQAQGEQSRALAES
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHH
VFMYATHIDALQKLGPMVSRIAHKHASLQVAPEHYPIVGKYLLEAIRDHLGLEDGHPVLG
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
AWSEAYAQLADIFITTEEGIYAANADKPGGWRGFRAFVIDEIRDEAQGIKSIYLVPEDGQ
CHHHHHHHHHHEEEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCC
AIADFEPGQYLGVKVQVPGHAYDEIRQYSLSDAPGKRTYRITVKAEGDAPAGKVSHHLHG
EEECCCCCCEEEEEEEECCCHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCHHHHCCCC
AEPGDRVWVQPPTGDFTVEREDRRLAFIAGGVGITPLLSMLHARAERGADLSDVVFVHCC
CCCCCEEEEECCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
RDKAHHAMADELRQLARAHGFSYRVAYERGEGGDHQGYLDRDVLTRWLGEPDADVYFCGP
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCEEEECC
RPFMAALNTALGEMGYREERLHYEVFGPSIRLQPH
CHHHHHHHHHHHHCCCHHHHCEEEEECCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10484179; 11058132 [H]