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The map label for this gene is pmbA [H]

Identifier: 74318166

GI number: 74318166

Start: 2235120

End: 2236460

Strand: Direct

Name: pmbA [H]

Synonym: Tbd_2148

Alternate gene names: 74318166

Gene position: 2235120-2236460 (Clockwise)

Preceding gene: 74318162

Following gene: 74318167

Centisome position: 76.81

GC content: 66.82

Gene sequence:

>1341_bases
ATGTCCCGCTCACAGTTCAGCTATACCCGTGACCAGCTCATTACCCTCTCCCGCATGGTCATCGAGCACGCCGTCGCCAA
GGGGGCGACCGCGGCCGACACCGAGATCTCGGAAGGCGTGGGCCAGAACGTCAGCGTGCGCCAGGGCGAAATCGAGACGA
TCGAATACAACAAGGACAAAGGCGCGGGCGTGACGGTCTACATCGGCCAGCGCCGCGGCCACGCGTCGACCTCCGATCTG
TCGGAATCGGCGCTGAAAAGCGCGGTCGAAAAGGCGCTGACGATCGCGCGCTACACCGCCGAGGACGACGCGGCCGGCCT
GCCCGACGCCGACCGGCTCGCGACCGACGTACCCGACCTCGACCTCTACCATCCGTGGACGCTCACGGTCGAAGAAGCGG
CCGAGACCGCGCGTGCCTGCGAGGCCGCCGCGCTCGCGGTAGACAAGCGCCTCACCAATTCGGAAGGCGCGGGCGTCTCG
ACCCACGCCTCGCATTTCATCTATGCCAACAGCCTCGGCTTCTGCAACGGCTACGCCGGCTCACGCCACGGCGTTTCGGT
CGCGGTGATCGGCGAGGACAAAGGCGCGATGCAGCGCGACTATTGGTACAGCAGCGCGCGCAATCCCGCCGACCTCGAGT
CCGCCGAGGCGGTCGGTCGCGTCGCCGGTGCGCGCACGGTGCGCCGGCTCAACGGCCGCAAGCTCGATACCCGCAGCTGC
CCGGTCGTATTCGAAGCGCCGATCGCGACCGGCCTGATCGGTAACTTCGTCGCGGCGGTCTCGGGCGGCAGCCTGTATCG
CAAATCTTCCTTCCTGCTCGACAGCCTCGGCACCGAGGTCTTCGCGCCGCTCGTGAACATCCGCGAGGTGCCTTTCCTTC
CGGCTGGCCTCGGCAGCGCGCCGTTCGACAGCGAAGGCGTCGCCTGCATCGAGCGCGACATCGTCCGGGACGGCGTCGTG
CAGGGCTATTTCCTGTCGAGCTATTCGGCGCGCAAGCTCGGTATGACGACGACCGGCAATGCGGGCGGCAGCCACAATCT
GGTCGTCGCGCCGGGCGAACTCGATCTCGACGGCCTTCTGAAGAAAATGGGCACGGGTCTGCTCGTCACCGAACTGCTCG
GCCAGGGCGCCAACATGGTGACCGGCGATTACTCGCGCGGCGCCGCCGGCTTCTGGGTCGAGAACGGCGAGATCCAGTAT
CCGGTCGAGGAAATCACGATCGCCGGCAATCTCAAGCAGATGTTCAAGGGCATCGTCGCGATCGGACGCGACGTCGAGCG
CCGCGGCTCCAAGCAGGTGGGGTCGATCCTCATCGACCGCATGACGGTGGCCGGCAACTGA

Upstream 100 bases:

>100_bases
CACGGCGGGTCTTTCGGTTGCTGTTATTATCGATATTGTAGTTTCTGCCCCGACGAACGCGCTGCGCAGGCGCATACCTC
CCACTTCCGACCAGCCGACC

Downstream 100 bases:

>100_bases
ATGTCGGCAGCCCGGCGATCGCTCGCTGCGCTCGTTGCCGTCTTCCTCGTCGTCGGCTGCGAACGGGCACCGACCCTGCC
CAAGCTCGGCCCCGACGACG

Product: microcin-processing peptidase 1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MSRSQFSYTRDQLITLSRMVIEHAVAKGATAADTEISEGVGQNVSVRQGEIETIEYNKDKGAGVTVYIGQRRGHASTSDL
SESALKSAVEKALTIARYTAEDDAAGLPDADRLATDVPDLDLYHPWTLTVEEAAETARACEAAALAVDKRLTNSEGAGVS
THASHFIYANSLGFCNGYAGSRHGVSVAVIGEDKGAMQRDYWYSSARNPADLESAEAVGRVAGARTVRRLNGRKLDTRSC
PVVFEAPIATGLIGNFVAAVSGGSLYRKSSFLLDSLGTEVFAPLVNIREVPFLPAGLGSAPFDSEGVACIERDIVRDGVV
QGYFLSSYSARKLGMTTTGNAGGSHNLVVAPGELDLDGLLKKMGTGLLVTELLGQGANMVTGDYSRGAAGFWVENGEIQY
PVEEITIAGNLKQMFKGIVAIGRDVERRGSKQVGSILIDRMTVAGN

Sequences:

>Translated_446_residues
MSRSQFSYTRDQLITLSRMVIEHAVAKGATAADTEISEGVGQNVSVRQGEIETIEYNKDKGAGVTVYIGQRRGHASTSDL
SESALKSAVEKALTIARYTAEDDAAGLPDADRLATDVPDLDLYHPWTLTVEEAAETARACEAAALAVDKRLTNSEGAGVS
THASHFIYANSLGFCNGYAGSRHGVSVAVIGEDKGAMQRDYWYSSARNPADLESAEAVGRVAGARTVRRLNGRKLDTRSC
PVVFEAPIATGLIGNFVAAVSGGSLYRKSSFLLDSLGTEVFAPLVNIREVPFLPAGLGSAPFDSEGVACIERDIVRDGVV
QGYFLSSYSARKLGMTTTGNAGGSHNLVVAPGELDLDGLLKKMGTGLLVTELLGQGANMVTGDYSRGAAGFWVENGEIQY
PVEEITIAGNLKQMFKGIVAIGRDVERRGSKQVGSILIDRMTVAGN
>Mature_445_residues
SRSQFSYTRDQLITLSRMVIEHAVAKGATAADTEISEGVGQNVSVRQGEIETIEYNKDKGAGVTVYIGQRRGHASTSDLS
ESALKSAVEKALTIARYTAEDDAAGLPDADRLATDVPDLDLYHPWTLTVEEAAETARACEAAALAVDKRLTNSEGAGVST
HASHFIYANSLGFCNGYAGSRHGVSVAVIGEDKGAMQRDYWYSSARNPADLESAEAVGRVAGARTVRRLNGRKLDTRSCP
VVFEAPIATGLIGNFVAAVSGGSLYRKSSFLLDSLGTEVFAPLVNIREVPFLPAGLGSAPFDSEGVACIERDIVRDGVVQ
GYFLSSYSARKLGMTTTGNAGGSHNLVVAPGELDLDGLLKKMGTGLLVTELLGQGANMVTGDYSRGAAGFWVENGEIQYP
VEEITIAGNLKQMFKGIVAIGRDVERRGSKQVGSILIDRMTVAGN

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=436, Percent_Identity=49.7706422018349, Blast_Score=437, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 47237; Mature: 47105

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSQFSYTRDQLITLSRMVIEHAVAKGATAADTEISEGVGQNVSVRQGEIETIEYNKDK
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCEEECCCEEEEEECCCC
GAGVTVYIGQRRGHASTSDLSESALKSAVEKALTIARYTAEDDAAGLPDADRLATDVPDL
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCC
DLYHPWTLTVEEAAETARACEAAALAVDKRLTNSEGAGVSTHASHFIYANSLGFCNGYAG
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCC
SRHGVSVAVIGEDKGAMQRDYWYSSARNPADLESAEAVGRVAGARTVRRLNGRKLDTRSC
CCCCEEEEEEECCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PVVFEAPIATGLIGNFVAAVSGGSLYRKSSFLLDSLGTEVFAPLVNIREVPFLPAGLGSA
CEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PFDSEGVACIERDIVRDGVVQGYFLSSYSARKLGMTTTGNAGGSHNLVVAPGELDLDGLL
CCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCEECCCCCCCCCEEEECCCCCHHHHH
KKMGTGLLVTELLGQGANMVTGDYSRGAAGFWVENGEIQYPVEEITIAGNLKQMFKGIVA
HHHCCCHHHHHHHCCCCCEEECCCCCCCCEEEEECCEEECCHHHEEEECCHHHHHHHHHH
IGRDVERRGSKQVGSILIDRMTVAGN
HCCCHHHHCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SRSQFSYTRDQLITLSRMVIEHAVAKGATAADTEISEGVGQNVSVRQGEIETIEYNKDK
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCEEECCCEEEEEECCCC
GAGVTVYIGQRRGHASTSDLSESALKSAVEKALTIARYTAEDDAAGLPDADRLATDVPDL
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCC
DLYHPWTLTVEEAAETARACEAAALAVDKRLTNSEGAGVSTHASHFIYANSLGFCNGYAG
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCC
SRHGVSVAVIGEDKGAMQRDYWYSSARNPADLESAEAVGRVAGARTVRRLNGRKLDTRSC
CCCCEEEEEEECCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PVVFEAPIATGLIGNFVAAVSGGSLYRKSSFLLDSLGTEVFAPLVNIREVPFLPAGLGSA
CEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PFDSEGVACIERDIVRDGVVQGYFLSSYSARKLGMTTTGNAGGSHNLVVAPGELDLDGLL
CCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCEECCCCCCCCCEEEECCCCCHHHHH
KKMGTGLLVTELLGQGANMVTGDYSRGAAGFWVENGEIQYPVEEITIAGNLKQMFKGIVA
HHHCCCHHHHHHHCCCCCEEECCCCCCCCEEEEECCEEECCHHHEEEECCHHHHHHHHHH
IGRDVERRGSKQVGSILIDRMTVAGN
HCCCHHHHCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]