Click here to switch to the map view.

The map label for this gene is tesA [H]

Identifier: 74318167

GI number: 74318167

Start: 2236461

End: 2237072

Strand: Direct

Name: tesA [H]

Synonym: Tbd_2149

Alternate gene names: 74318167

Gene position: 2236461-2237072 (Clockwise)

Preceding gene: 74318166

Following gene: 74318168

Centisome position: 76.86

GC content: 68.95

Gene sequence:

>612_bases
ATGTCGGCAGCCCGGCGATCGCTCGCTGCGCTCGTTGCCGTCTTCCTCGTCGTCGGCTGCGAACGGGCACCGACCCTGCC
CAAGCTCGGCCCCGACGACGTCATCGTCGCCTTCGGTGACAGCCTCACCTTCGGTACCGGCGCGAGCCGTGAAACCGCCT
ACCCCGCAGTGCTCGCCACGCTCACCGGCCGTACCGTCGTCAATGCCGGCGTTCCGGGCGACACGACGGCATCTGCCCTG
CAACGCCTGCCCGCGGTGCTCGATCAACACCGGCCTCGTCTCGTTCTGCTCTGCCTCGGCGGCAACGACATGCTTCGAAA
ACAGCCCGAAGCCGACACCGAAAACAACCTGCGCCGCCTTGTCGCGTCGATCCGTGCGAGCGGCGCCGAGGTGATGCTGA
TCGCGGTCCCGGCGCCCACACTCTTCGGCCGACCGCCCGCGTTCTATGCGCGCGTCGCGGACGACCTGAAGCTGCCGCTC
GAGGACGACGCCTTCGACGCCGTGCTCCGCGACAACCGCCTGAAGGCCGACCCGATTCACGCCAACGCCGCCGGCTATCG
CGTCGTCGCCGAGCAGCTGGCCGATTTTCTCGACGACGCCGGCGCGCTATGA

Upstream 100 bases:

>100_bases
GTTCAAGGGCATCGTCGCGATCGGACGCGACGTCGAGCGCCGCGGCTCCAAGCAGGTGGGGTCGATCCTCATCGACCGCA
TGACGGTGGCCGGCAACTGA

Downstream 100 bases:

>100_bases
AAACGCTGCTTGCGCTGGCCGGCCGGATCGACGCACTGACCGAACGCGTCGGGCGCATCGTGATCTGGCTGGTTCTCGTG
GCCACGCTCATTTCCGCCGG

Product: putative lipase

Products: NA

Alternate protein names: Lecithinase B; Lysophospholipase L1; Protease I [H]

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MSAARRSLAALVAVFLVVGCERAPTLPKLGPDDVIVAFGDSLTFGTGASRETAYPAVLATLTGRTVVNAGVPGDTTASAL
QRLPAVLDQHRPRLVLLCLGGNDMLRKQPEADTENNLRRLVASIRASGAEVMLIAVPAPTLFGRPPAFYARVADDLKLPL
EDDAFDAVLRDNRLKADPIHANAAGYRVVAEQLADFLDDAGAL

Sequences:

>Translated_203_residues
MSAARRSLAALVAVFLVVGCERAPTLPKLGPDDVIVAFGDSLTFGTGASRETAYPAVLATLTGRTVVNAGVPGDTTASAL
QRLPAVLDQHRPRLVLLCLGGNDMLRKQPEADTENNLRRLVASIRASGAEVMLIAVPAPTLFGRPPAFYARVADDLKLPL
EDDAFDAVLRDNRLKADPIHANAAGYRVVAEQLADFLDDAGAL
>Mature_202_residues
SAARRSLAALVAVFLVVGCERAPTLPKLGPDDVIVAFGDSLTFGTGASRETAYPAVLATLTGRTVVNAGVPGDTTASALQ
RLPAVLDQHRPRLVLLCLGGNDMLRKQPEADTENNLRRLVASIRASGAEVMLIAVPAPTLFGRPPAFYARVADDLKLPLE
DDAFDAVLRDNRLKADPIHANAAGYRVVAEQLADFLDDAGAL

Specific function: Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=174, Percent_Identity=35.632183908046, Blast_Score=100, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 21439; Mature: 21308

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAARRSLAALVAVFLVVGCERAPTLPKLGPDDVIVAFGDSLTFGTGASRETAYPAVLAT
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCEECCCCCCCCCHHHHHHHH
LTGRTVVNAGVPGDTTASALQRLPAVLDQHRPRLVLLCLGGNDMLRKQPEADTENNLRRL
HCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHCCCCCCHHHHHHHH
VASIRASGAEVMLIAVPAPTLFGRPPAFYARVADDLKLPLEDDAFDAVLRDNRLKADPIH
HHHHHCCCCEEEEEEECCCHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCC
ANAAGYRVVAEQLADFLDDAGAL
CCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SAARRSLAALVAVFLVVGCERAPTLPKLGPDDVIVAFGDSLTFGTGASRETAYPAVLAT
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCEECCCCCCCCCHHHHHHHH
LTGRTVVNAGVPGDTTASALQRLPAVLDQHRPRLVLLCLGGNDMLRKQPEADTENNLRRL
HCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHCCCCCCHHHHHHHH
VASIRASGAEVMLIAVPAPTLFGRPPAFYARVADDLKLPLEDDAFDAVLRDNRLKADPIH
HHHHHCCCCEEEEEEECCCHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCC
ANAAGYRVVAEQLADFLDDAGAL
CCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]