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The map label for this gene is trpB

Identifier: 74317932

GI number: 74317932

Start: 2009757

End: 2010956

Strand: Reverse

Name: trpB

Synonym: Tbd_1914

Alternate gene names: 74317932

Gene position: 2010956-2009757 (Counterclockwise)

Preceding gene: 74317933

Following gene: 74317931

Centisome position: 69.11

GC content: 66.67

Gene sequence:

>1200_bases
ATGAAGCTGACTGACCTTCCCGATTCCCGCGGCCACTTCGGTCCCTACGGCGGCATTTTCGTTTCCGAAACGCTGATGGC
CGCGCTCGACGCGCTGCGTGTCGAATACGACGCGGCGTGCCGGGACCCCGGCTTCATGGCCGAATTCGAGTACGAACTCA
AGCACTACGTCGGGCGGCCGAGCCCGGTCTACCACGCCCGGCGGCTTTCGGAGGAATACGGCGGCGCGCAGATCTACCTC
AAGCGCGAGGATCTCAACCACACCGGCGCGCACAAGATCAACAACACGATCGGCCAGGCGCTGCTCGCGCGCCGCATGGG
CAAGAAGCGCGTCATCGCCGAGACCGGCGCGGGCCAGCACGGCGTCGCGTCGGCGACCGTCGCCGCGCGCTACGGCATGG
AATGCGTTGTCTACATGGGCGCCGAAGACGTCGCGCGACAGGCCCCCAACGTCTTTCGTATGAAGCTCCTCGGCGCGACC
GTCGTGCCCGTGTCGTCGGGTTCGAAGACGCTGAAGGACGCGCTGAACGAAGCGATGCGCGACTGGGTGACGAACGTCGA
GTCGACCTTCTACATCCTCGGCACCGCGGCCGGCCCGCATCCCTACCCGATGCTCGTGCGCGACTTCCAGTGCGTGATCG
GGCGCGAATGCATCGCGCAGATGCCCGAGCTCGTCGGACGCCAGCCCGACGCGGTCGTCGCCTGCGTCGGCGGCGGCTCG
AACGCGATCGGAATTTTCCATCCCTACATTCCCCATGAGAACGTGCGCCTGATCGGTGTCGAAGCCGGCGGTTCGGGGGT
CGCGAGCGGCAAGCACGCTGCGCCGCTGACCGCCGGCACGCCCGGGGTGTTGCACGGCTTTCGCAGCTACCTGATGCAGG
ACGAGAACGGCCAGATCATCGAGACCCATTCGGTCTCGGCCGGCCTCGACTATCCGGGCGTCGGCCCCGAGCACAGCTAT
CTCAAGGACGCCGGTCGCGCCGAATACGTGCCGATCAACGACGACGAAGCGCTCGCCGCCTTCCACGATCTGTGCCGCTT
CGAGGGCATCATCCCCGCGCTCGAGTCGAGCCACGCGGTGGCGCAGGCGAAGAAACTCGCGCCGACGATGAAGAAGGACC
AGGTCATTCTGGTGAACCTCTCGGGGCGCGGCGACAAGGACATCAACACCGTGGCGAAGGCGGCGGGCATCACGCTCTGA

Upstream 100 bases:

>100_bases
TCCGTCCCTGGGCGGTCGACGTGTCCTCGGGCGTCGAGGCGGCGAAAGGCATCAAGGACGCGCAGAAGATCGCGCGATTC
ATCTCACAGGCGAAAGCATC

Downstream 100 bases:

>100_bases
GTTGCCGCGTCACACTCCCCGGTCTTTAGAAAGCAACGAATCCATGAGCCGCATCGGCCAGACGTTCACCGCGCTCAAGG
CGCAAAACAGGAAAGCCCTG

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MKLTDLPDSRGHFGPYGGIFVSETLMAALDALRVEYDAACRDPGFMAEFEYELKHYVGRPSPVYHARRLSEEYGGAQIYL
KREDLNHTGAHKINNTIGQALLARRMGKKRVIAETGAGQHGVASATVAARYGMECVVYMGAEDVARQAPNVFRMKLLGAT
VVPVSSGSKTLKDALNEAMRDWVTNVESTFYILGTAAGPHPYPMLVRDFQCVIGRECIAQMPELVGRQPDAVVACVGGGS
NAIGIFHPYIPHENVRLIGVEAGGSGVASGKHAAPLTAGTPGVLHGFRSYLMQDENGQIIETHSVSAGLDYPGVGPEHSY
LKDAGRAEYVPINDDEALAAFHDLCRFEGIIPALESSHAVAQAKKLAPTMKKDQVILVNLSGRGDKDINTVAKAAGITL

Sequences:

>Translated_399_residues
MKLTDLPDSRGHFGPYGGIFVSETLMAALDALRVEYDAACRDPGFMAEFEYELKHYVGRPSPVYHARRLSEEYGGAQIYL
KREDLNHTGAHKINNTIGQALLARRMGKKRVIAETGAGQHGVASATVAARYGMECVVYMGAEDVARQAPNVFRMKLLGAT
VVPVSSGSKTLKDALNEAMRDWVTNVESTFYILGTAAGPHPYPMLVRDFQCVIGRECIAQMPELVGRQPDAVVACVGGGS
NAIGIFHPYIPHENVRLIGVEAGGSGVASGKHAAPLTAGTPGVLHGFRSYLMQDENGQIIETHSVSAGLDYPGVGPEHSY
LKDAGRAEYVPINDDEALAAFHDLCRFEGIIPALESSHAVAQAKKLAPTMKKDQVILVNLSGRGDKDINTVAKAAGITL
>Mature_399_residues
MKLTDLPDSRGHFGPYGGIFVSETLMAALDALRVEYDAACRDPGFMAEFEYELKHYVGRPSPVYHARRLSEEYGGAQIYL
KREDLNHTGAHKINNTIGQALLARRMGKKRVIAETGAGQHGVASATVAARYGMECVVYMGAEDVARQAPNVFRMKLLGAT
VVPVSSGSKTLKDALNEAMRDWVTNVESTFYILGTAAGPHPYPMLVRDFQCVIGRECIAQMPELVGRQPDAVVACVGGGS
NAIGIFHPYIPHENVRLIGVEAGGSGVASGKHAAPLTAGTPGVLHGFRSYLMQDENGQIIETHSVSAGLDYPGVGPEHSY
LKDAGRAEYVPINDDEALAAFHDLCRFEGIIPALESSHAVAQAKKLAPTMKKDQVILVNLSGRGDKDINTVAKAAGITL

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=381, Percent_Identity=58.005249343832, Blast_Score=446, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6321412, Length=382, Percent_Identity=58.6387434554974, Blast_Score=466, Evalue=1e-132,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_THIDA (Q3SHL9)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315672.1
- HSSP:   P0A2K1
- ProteinModelPortal:   Q3SHL9
- SMR:   Q3SHL9
- STRING:   Q3SHL9
- GeneID:   3674024
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1914
- NMPDR:   fig|292415.3.peg.2011
- eggNOG:   COG0133
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- PhylomeDB:   Q3SHL9
- ProtClustDB:   PRK04346
- BioCyc:   TDEN292415:TBD_1914-MONOMER
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 42799; Mature: 42799

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTDLPDSRGHFGPYGGIFVSETLMAALDALRVEYDAACRDPGFMAEFEYELKHYVGRP
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
SPVYHARRLSEEYGGAQIYLKREDLNHTGAHKINNTIGQALLARRMGKKRVIAETGAGQH
CHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
GVASATVAARYGMECVVYMGAEDVARQAPNVFRMKLLGATVVPVSSGSKTLKDALNEAMR
CCHHHHHHHHCCCEEEEEECCHHHHHHCCCCEEHHHHCCEEEEECCCHHHHHHHHHHHHH
DWVTNVESTFYILGTAAGPHPYPMLVRDFQCVIGRECIAQMPELVGRQPDAVVACVGGGS
HHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
NAIGIFHPYIPHENVRLIGVEAGGSGVASGKHAAPLTAGTPGVLHGFRSYLMQDENGQII
CEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEECCCCCEE
ETHSVSAGLDYPGVGPEHSYLKDAGRAEYVPINDDEALAAFHDLCRFEGIIPALESSHAV
EECCCCCCCCCCCCCCCHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHCCCHHHCCHHHH
AQAKKLAPTMKKDQVILVNLSGRGDKDINTVAKAAGITL
HHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKLTDLPDSRGHFGPYGGIFVSETLMAALDALRVEYDAACRDPGFMAEFEYELKHYVGRP
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
SPVYHARRLSEEYGGAQIYLKREDLNHTGAHKINNTIGQALLARRMGKKRVIAETGAGQH
CHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
GVASATVAARYGMECVVYMGAEDVARQAPNVFRMKLLGATVVPVSSGSKTLKDALNEAMR
CCHHHHHHHHCCCEEEEEECCHHHHHHCCCCEEHHHHCCEEEEECCCHHHHHHHHHHHHH
DWVTNVESTFYILGTAAGPHPYPMLVRDFQCVIGRECIAQMPELVGRQPDAVVACVGGGS
HHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
NAIGIFHPYIPHENVRLIGVEAGGSGVASGKHAAPLTAGTPGVLHGFRSYLMQDENGQII
CEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEECCCCCEE
ETHSVSAGLDYPGVGPEHSYLKDAGRAEYVPINDDEALAAFHDLCRFEGIIPALESSHAV
EECCCCCCCCCCCCCCCHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHCCCHHHCCHHHH
AQAKKLAPTMKKDQVILVNLSGRGDKDINTVAKAAGITL
HHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA