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The map label for this gene is trpA

Identifier: 74317931

GI number: 74317931

Start: 2008892

End: 2009713

Strand: Reverse

Name: trpA

Synonym: Tbd_1913

Alternate gene names: 74317931

Gene position: 2009713-2008892 (Counterclockwise)

Preceding gene: 74317932

Following gene: 74317930

Centisome position: 69.07

GC content: 65.82

Gene sequence:

>822_bases
ATGAGCCGCATCGGCCAGACGTTCACCGCGCTCAAGGCGCAAAACAGGAAAGCCCTGATTTCCTTCATCACGGCCGGCGA
TCCCGGCAAGGGTCTGACCGTGCCGCTCATGCATGCGCTCGTCGACGCCGGCACCGACATCATCGAGCTCGGCGTGCCGT
TTTCCGACCCCATGGCCGACGGTCCGGTGATCCAGCGCGCGTCGGAACGGGCGCTCGCGAACAAGGTCGGGCTCAAGGAC
GTGCTGGCGATGACCGCCGAGTTCCGCAAGGTCAACACCAGCACGCCCGTCGTCCTGATGGGTTACGCCAACCCGTTTGA
GCACATGGGTTACGAGACCTTTGCCGAGGCCGCGAAGACCGCGGGCGTCGACGGTGTGCTGACCGTCGACATTCCGCCCG
AGGAATCGCTCGGCGTGTCCGACGTGTTCAAGGCCCACGACCTGGACCCGATTTTCCTGCTGTCGCCGACCACGCCCGAG
TCGCGTGTTGCGCAGGTAGCCGCGGTCGCCGGCGGCTTCATCTATTACGTGTCGCTCAAGGGGGTGACCGGCTCGGCCAA
CCTCGACACCGAGGAGGTCGCGCGCAAGGTGGCGATGGTGCGTAAGCATTGTGACTTGCCGGTCGGCGTCGGTTTCGGCA
TCCGCGACGCGGCCACTGCCGAAGCCGTCGCGCGCATTGCCGATGCGGTCGTGGTGGGGAGCCGCATTGTCAACGAGATC
GAGACCGCGCCCGAGGGAGAATGCGGCGCGCGGGTCAAGCATCTGGTCGCCGACCTGCGCCGCGGCGTCGACGCCGCATC
GAAGCAAACGGAGAATAAATAA

Upstream 100 bases:

>100_bases
GGGCGCGGCGACAAGGACATCAACACCGTGGCGAAGGCGGCGGGCATCACGCTCTGAGTTGCCGCGTCACACTCCCCGGT
CTTTAGAAAGCAACGAATCC

Downstream 100 bases:

>100_bases
TGAGCTGGTTCCAGAAAATCCTGCCGCCCAAGATCAAGCGTCGCGCGCAGACGTCGAAGACCATGCCCGAGGGCCTGTGG
TCGAAGTGCCCGGCGTGCAA

Product: tryptophan synthase, subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSRIGQTFTALKAQNRKALISFITAGDPGKGLTVPLMHALVDAGTDIIELGVPFSDPMADGPVIQRASERALANKVGLKD
VLAMTAEFRKVNTSTPVVLMGYANPFEHMGYETFAEAAKTAGVDGVLTVDIPPEESLGVSDVFKAHDLDPIFLLSPTTPE
SRVAQVAAVAGGFIYYVSLKGVTGSANLDTEEVARKVAMVRKHCDLPVGVGFGIRDAATAEAVARIADAVVVGSRIVNEI
ETAPEGECGARVKHLVADLRRGVDAASKQTENK

Sequences:

>Translated_273_residues
MSRIGQTFTALKAQNRKALISFITAGDPGKGLTVPLMHALVDAGTDIIELGVPFSDPMADGPVIQRASERALANKVGLKD
VLAMTAEFRKVNTSTPVVLMGYANPFEHMGYETFAEAAKTAGVDGVLTVDIPPEESLGVSDVFKAHDLDPIFLLSPTTPE
SRVAQVAAVAGGFIYYVSLKGVTGSANLDTEEVARKVAMVRKHCDLPVGVGFGIRDAATAEAVARIADAVVVGSRIVNEI
ETAPEGECGARVKHLVADLRRGVDAASKQTENK
>Mature_272_residues
SRIGQTFTALKAQNRKALISFITAGDPGKGLTVPLMHALVDAGTDIIELGVPFSDPMADGPVIQRASERALANKVGLKDV
LAMTAEFRKVNTSTPVVLMGYANPFEHMGYETFAEAAKTAGVDGVLTVDIPPEESLGVSDVFKAHDLDPIFLLSPTTPES
RVAQVAAVAGGFIYYVSLKGVTGSANLDTEEVARKVAMVRKHCDLPVGVGFGIRDAATAEAVARIADAVVVGSRIVNEIE
TAPEGECGARVKHLVADLRRGVDAASKQTENK

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=214, Percent_Identity=35.981308411215, Blast_Score=141, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6321412, Length=246, Percent_Identity=41.0569105691057, Blast_Score=193, Evalue=3e-50,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_THIDA (Q3SHM0)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315671.1
- HSSP:   P42390
- ProteinModelPortal:   Q3SHM0
- SMR:   Q3SHM0
- STRING:   Q3SHM0
- GeneID:   3674023
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1913
- NMPDR:   fig|292415.3.peg.2010
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- PhylomeDB:   Q3SHM0
- BioCyc:   TDEN292415:TBD_1913-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28742; Mature: 28611

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIGQTFTALKAQNRKALISFITAGDPGKGLTVPLMHALVDAGTDIIELGVPFSDPMAD
CCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPVIQRASERALANKVGLKDVLAMTAEFRKVNTSTPVVLMGYANPFEHMGYETFAEAAKT
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHCCHHHHHHHHHH
AGVDGVLTVDIPPEESLGVSDVFKAHDLDPIFLLSPTTPESRVAQVAAVAGGFIYYVSLK
CCCCCEEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEEEEE
GVTGSANLDTEEVARKVAMVRKHCDLPVGVGFGIRDAATAEAVARIADAVVVGSRIVNEI
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ETAPEGECGARVKHLVADLRRGVDAASKQTENK
HCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCC
>Mature Secondary Structure 
SRIGQTFTALKAQNRKALISFITAGDPGKGLTVPLMHALVDAGTDIIELGVPFSDPMAD
CCHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPVIQRASERALANKVGLKDVLAMTAEFRKVNTSTPVVLMGYANPFEHMGYETFAEAAKT
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHCCHHHHHHHHHH
AGVDGVLTVDIPPEESLGVSDVFKAHDLDPIFLLSPTTPESRVAQVAAVAGGFIYYVSLK
CCCCCEEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEEEEE
GVTGSANLDTEEVARKVAMVRKHCDLPVGVGFGIRDAATAEAVARIADAVVVGSRIVNEI
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ETAPEGECGARVKHLVADLRRGVDAASKQTENK
HCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA