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The map label for this gene is amiC [H]

Identifier: 74317537

GI number: 74317537

Start: 1606593

End: 1607849

Strand: Reverse

Name: amiC [H]

Synonym: Tbd_1519

Alternate gene names: 74317537

Gene position: 1607849-1606593 (Counterclockwise)

Preceding gene: 74317539

Following gene: 74317536

Centisome position: 55.26

GC content: 66.11

Gene sequence:

>1257_bases
ATGCGTGGCCGCGAGCGCGTTGGGGGGTCGATATCTGGAGAGGTGCTGCGCGCCGTGTTAAGAATCTTCCTGCTGTGTGC
CGTGTTGTTGCCGGGCTGGGCGCAGGCGCTCCAACTTTCTGCGAGTCGGGTCTGGCCCTCCCCGGACTACACCCGCGTCA
CCCTCGAAGCGCAGGCGCCCGTCGTCCATACCTATTTCACCCTTGCCAAGCCCGACCGGCTCGTGATCGATCTCGAGGGC
GTCGAGGCCGGTCCGGCGCTCGACGCGCTGATGACGCAGCTGAGCGCTGACGATCCTTACGTCGGCGCGATCCGCAGCGG
CGTGAACCGGCCCGGTGTGATGCGTCTGGTGCTCGAACTCAAGGCCGCGGTCCGGCCCTCGATCTTCCAATTGCCCCCCC
TCGGGGAGTATGGCCATCGGCTCGTGATCGACCTGTACCCGGCCGAGGCGCGCGCTGCGGCCGTCAGCCCGGCGGCGCTC
CATCCCGAAGGCCGCACGCCGCAGAAGGCGGCCAAGCCCGAAGCGGCCGGGCAGTACGTGCGGCTGATCACGGTCGCGAT
CGACGCGGGCCACGGCGGCGAGGACCCCGGCGCACTCGGCGCCGCCGGCTCACGGGAGAAAGACATCACGCTCGCGCTGG
CGAAGAAGCTCAAGCAGAAGATCGACGCCCAGGAAAACATGCACGCGGTGCTGACCCGCGACGGCGATTATTTCGTGCCG
CTCGGACAGCGCGTGACCAAGGCGCGCTCGTTCAAGGCCGACCTCTTTCTGTCGATCCACGCCGACGCCTTCATCAAGCC
GCACGCCCGCGGGTCGTCGGTGTTCGCGCTGTCCGAGAACGGGGCGACCTCGGTCGCGGCGCGCTGGCTCGCCAGGAAGG
AAAACGAGGCCGACCTGATCGGTGGCATCAACATCGACGTCAAGGATCCGTTCCTCAAGCGTACGCTGCTCGACCTGTCG
CAGACTGCGACGATCAACGACAGTCTCAAGCTTGGCCACGCCGTGCTCAAGGAAATCGGCGGCGTCAACACCTTGCACAA
GGCGCAGGTCGAACAGGCAGGGTTCGCCGTGTTGAAGGCGCCCGACATCCCGTCAATCCTGATCGAGACCGCGTTCATTT
CCAATCCCGAAGAAGAAAAGCGCCTCAACGACGCGGCCTACCAGGACAAGCTGGTCGATGCGATCGTCGTCGGCGTCAAG
GATTATTTCGCCAGACACCCGCTGACCGGGCCGTCGCGGCTGACGCGCAATCCATGA

Upstream 100 bases:

>100_bases
CGGTCAGCGTGATCGAGTGGCCCGAGAAAGCGGCAGGCTGGCTGCCGGCGCCCGACGTGATCCTGCGTCTGGCGATCTTG
GACGAGACGCGCGAAATCGA

Downstream 100 bases:

>100_bases
CCGCGAGCCTGCTCGGCGCGGCGGCCCCGGGATTCGATCGGCCGCTTGAGGTGCTGGAGGCCTGCCATGCGCGCATCCTC
AAGCAATGCGCGACCCTCGA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MRGRERVGGSISGEVLRAVLRIFLLCAVLLPGWAQALQLSASRVWPSPDYTRVTLEAQAPVVHTYFTLAKPDRLVIDLEG
VEAGPALDALMTQLSADDPYVGAIRSGVNRPGVMRLVLELKAAVRPSIFQLPPLGEYGHRLVIDLYPAEARAAAVSPAAL
HPEGRTPQKAAKPEAAGQYVRLITVAIDAGHGGEDPGALGAAGSREKDITLALAKKLKQKIDAQENMHAVLTRDGDYFVP
LGQRVTKARSFKADLFLSIHADAFIKPHARGSSVFALSENGATSVAARWLARKENEADLIGGINIDVKDPFLKRTLLDLS
QTATINDSLKLGHAVLKEIGGVNTLHKAQVEQAGFAVLKAPDIPSILIETAFISNPEEEKRLNDAAYQDKLVDAIVVGVK
DYFARHPLTGPSRLTRNP

Sequences:

>Translated_418_residues
MRGRERVGGSISGEVLRAVLRIFLLCAVLLPGWAQALQLSASRVWPSPDYTRVTLEAQAPVVHTYFTLAKPDRLVIDLEG
VEAGPALDALMTQLSADDPYVGAIRSGVNRPGVMRLVLELKAAVRPSIFQLPPLGEYGHRLVIDLYPAEARAAAVSPAAL
HPEGRTPQKAAKPEAAGQYVRLITVAIDAGHGGEDPGALGAAGSREKDITLALAKKLKQKIDAQENMHAVLTRDGDYFVP
LGQRVTKARSFKADLFLSIHADAFIKPHARGSSVFALSENGATSVAARWLARKENEADLIGGINIDVKDPFLKRTLLDLS
QTATINDSLKLGHAVLKEIGGVNTLHKAQVEQAGFAVLKAPDIPSILIETAFISNPEEEKRLNDAAYQDKLVDAIVVGVK
DYFARHPLTGPSRLTRNP
>Mature_418_residues
MRGRERVGGSISGEVLRAVLRIFLLCAVLLPGWAQALQLSASRVWPSPDYTRVTLEAQAPVVHTYFTLAKPDRLVIDLEG
VEAGPALDALMTQLSADDPYVGAIRSGVNRPGVMRLVLELKAAVRPSIFQLPPLGEYGHRLVIDLYPAEARAAAVSPAAL
HPEGRTPQKAAKPEAAGQYVRLITVAIDAGHGGEDPGALGAAGSREKDITLALAKKLKQKIDAQENMHAVLTRDGDYFVP
LGQRVTKARSFKADLFLSIHADAFIKPHARGSSVFALSENGATSVAARWLARKENEADLIGGINIDVKDPFLKRTLLDLS
QTATINDSLKLGHAVLKEIGGVNTLHKAQVEQAGFAVLKAPDIPSILIETAFISNPEEEKRLNDAAYQDKLVDAIVVGVK
DYFARHPLTGPSRLTRNP

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=389, Percent_Identity=46.5295629820051, Blast_Score=344, Evalue=5e-96,
Organism=Escherichia coli, GI1788776, Length=239, Percent_Identity=47.6987447698745, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1790611, Length=231, Percent_Identity=41.5584415584416, Blast_Score=186, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 45013; Mature: 45013

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGRERVGGSISGEVLRAVLRIFLLCAVLLPGWAQALQLSASRVWPSPDYTRVTLEAQAP
CCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEEEECCC
VVHTYFTLAKPDRLVIDLEGVEAGPALDALMTQLSADDPYVGAIRSGVNRPGVMRLVLEL
EEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHH
KAAVRPSIFQLPPLGEYGHRLVIDLYPAEARAAAVSPAALHPEGRTPQKAAKPEAAGQYV
HHHHCCCCCCCCCCCCCCCEEEEEEECCCCHHHHCCCHHCCCCCCCCHHHCCCHHHCCEE
RLITVAIDAGHGGEDPGALGAAGSREKDITLALAKKLKQKIDAQENMHAVLTRDGDYFVP
EEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHCCCEEEEECCCCEEEC
LGQRVTKARSFKADLFLSIHADAFIKPHARGSSVFALSENGATSVAARWLARKENEADLI
CHHHHHHHHCCCEEEEEEEECCCEECCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCEE
GGINIDVKDPFLKRTLLDLSQTATINDSLKLGHAVLKEIGGVNTLHKAQVEQAGFAVLKA
ECEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEEEC
PDIPSILIETAFISNPEEEKRLNDAAYQDKLVDAIVVGVKDYFARHPLTGPSRLTRNP
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCC
>Mature Secondary Structure
MRGRERVGGSISGEVLRAVLRIFLLCAVLLPGWAQALQLSASRVWPSPDYTRVTLEAQAP
CCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEEEECCC
VVHTYFTLAKPDRLVIDLEGVEAGPALDALMTQLSADDPYVGAIRSGVNRPGVMRLVLEL
EEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHH
KAAVRPSIFQLPPLGEYGHRLVIDLYPAEARAAAVSPAALHPEGRTPQKAAKPEAAGQYV
HHHHCCCCCCCCCCCCCCCEEEEEEECCCCHHHHCCCHHCCCCCCCCHHHCCCHHHCCEE
RLITVAIDAGHGGEDPGALGAAGSREKDITLALAKKLKQKIDAQENMHAVLTRDGDYFVP
EEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHCCCEEEEECCCCEEEC
LGQRVTKARSFKADLFLSIHADAFIKPHARGSSVFALSENGATSVAARWLARKENEADLI
CHHHHHHHHCCCEEEEEEEECCCEECCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCEE
GGINIDVKDPFLKRTLLDLSQTATINDSLKLGHAVLKEIGGVNTLHKAQVEQAGFAVLKA
ECEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEEEC
PDIPSILIETAFISNPEEEKRLNDAAYQDKLVDAIVVGVKDYFARHPLTGPSRLTRNP
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]