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The map label for this gene is hypF [H]

Identifier: 74317403

GI number: 74317403

Start: 1463375

End: 1465636

Strand: Direct

Name: hypF [H]

Synonym: Tbd_1385

Alternate gene names: 74317403

Gene position: 1463375-1465636 (Clockwise)

Preceding gene: 74317402

Following gene: 74317404

Centisome position: 50.29

GC content: 70.34

Gene sequence:

>2262_bases
ATGCCTGAGCGCATCGCGCAATTCATCACGATCGGCGGGCGCGTGCAGGGGGTCGGCTTTCGGCCTTTCGTCTACCGCGC
CGCGCGCGCGCACGCGCTTGCAGGATGGGTGCGCAACGCTAACGGATCGGTGGAAATTCACGTCGAAGGCGAACGCGCGC
AGTTGCAAGGTTTCGTCGACACGCTGCTGAACGAGGCGCCGCCGCTGTCGGCGCCCGGCCCGCTAGCGATCCGGCCGTGC
GCGTCCACCCGGCTTCGCGGCTTCTCGATTCTCGACAGCACGGCGGCGAGCGAAGCCGACGTCCATCTTCCGGCAGACGG
TTTCGTCTGCGCCGACTGCCTCAACGAACTGCACGATCCGGCCAACCGCCGCTACCGCTACCCCTTCATCAACTGCACGC
AGTGCGGCCCGCGCTACACGCTCATCACCGGCCTGCCCTACGACCGGCCAATGACCGCGATGCGCGGTTTCACGCTGTGC
GCGGCGTGCCGGCGCGAATACGAGGACGCGGGCGACCGCCGTTTCCATGCCGAGCCAATCGCGTGCCCGGTGTGCGGGCC
GCATCTGCAATTCGTGTCGGGCGACAAGACCCTCAGCGGTGACGAGACGGCACTGGCAGCCGCCGTGGCCCAGCTGCGCG
CAGGAAAAATCGTTGCGGTGAAAGGCGTCGGCGGCTACCACCTGATGTGCGACGCGCGCAACGACCCGGCCGTCGCCGCA
CTCCGTGCGCGCAAGCCGCGGCCGGCCAAACCGCTCGCCGTGATGTTTCGCGACCTCGACAGCCTGCGCGACGCGGTCCA
TTTCGACACGGACGACGCGGCGCTGCTGAGCTCGCCCGCGCGGCCGATCGTGCTGCTGCGGAAGCGCAACACAAACCCGC
TTTCAGCTGCCATTGCGCCGGGCCTCGGCGAAATCGGCTGCATGCTCCCCTACTCGCCCCTGCACGATCTCCTGCTGCAC
GATTTCGGCGGACCGCTCGTCGCAAGCTCCGGCAACCTTTCGGGCGAACCGGTGCTGACCGCCAATGCCGAGGCACAGGC
GCGGCTGAGGCATCTCGCCGACGCCTTCCTGCATCACGACCGGCCGATCGTGCGCCCCGCCGACGACCCGGTCTACCGCC
GAATCGCCGGCGCGCCACGGCCCTTGCGCCTCGGCCGCGGCCTCGCACCGCTCGAACTCGAACTGCCCGCCGCACTCGCG
CAGCCCGTTCTCGCGCTCGGCAGCCACATGAAAAACACGGTCTGCCTCGCCTGGGGAACGCGCGCGGTCGTCTCGCCGCA
CATCGGAGAACTCGACAGCGCACGCAGTCTGGACACGCTCGCCCGGGTCGCCGCCGATCTGCAAGACCTGTATCAGGTTC
GCGCCGCGACGCTGCTTGTCGATCATCACCCCGGCTTCGGCTACCGCCGCTTCGCGCGCGACAGTGGCCTGCCTCTCGCT
GCGGTCTGGCATCACCACGCGCACGCCTCCGCGCTCGCGGCCGAGTTTGAGGATATTTCGGAGTGGATCGTCTTCGCCTG
GGATGGGGTCGGATTGGGCGAAGACATGAGCCTGTGGGGCGGCGAAGCCTTCGCCGGCGCGCCCGGGCGCTGGCGGCGCG
CCGCCTCCTGGCGCCCGTTCCGGCTCCCCGGCGGGGACAAGGCCGGACGTGAACCCTGGCGCGCGGCGGCGGCACTGCTC
TGGGAAACTGGCGCGCCGGCGGCCTTCGCGCCACCCGCCCTGCACCAGGCGTGGGCGGCGCGGCTCAACAGCCCGGGCAG
CTCGTCGGTCGGCCGGCTCCTCGACGCGGCGGCAGCGCTCTGCGGAATCTGTACCGAGGCGAGCTTCGAGGGCGAGGCGC
CGATGCGTATCGAAGCGTGCGCAAGCGATATCGCACCCGACGGCACGTGCATCGACGCCGCGGCGCTCACGCTGCCGCTC
GCCGCCGACGCCCAAGGCGTCTGGCGCAGCGACTGGGCCCCGTTGCTGCCTATGCTTGCCGACGCGACCCAGCCGGCCTC
GCGCCGCGCTGCCCGCTTCCACCTGAGCCTCGCCGAAGCACTGGTCGCGCAGGCGAAGCAGCTGCGCGGCGATACCGGCA
TCCGCACCGTCGGCCTGACCGGCGGCGTTTTCCAGAACCGGCTGCTCACCGAGACCGCGGCGCTGGCCCTCGCGGCGGCG
GGATTTTCGGTCCGCCTGCCGCAGCGCGTACCGATGAACGACGCCGGCCTGAGCTTCGGCCAGGTCATCGAGTTCCTATA
CCGACAACACACCAAGACATGA

Upstream 100 bases:

>100_bases
GGCAAGGCCTGCACGCCGCGCAAGCCGGTGGGACCGTGCATGGTCTCGGACGAAGGCGCCTGCCGCATCTGGTGGGCCGG
TGGCGTGCGCGTCGCGGCCG

Downstream 100 bases:

>100_bases
ACGAAGAGACCGCTCGCATCCTCCTCGCACACGGCAACGGGGGGCGCCTCATGCGCGAGCTGATCGCCGGCATATTCGCG
CGCCACCTCGGCAATCCGCT

Product: hydrogenase maturation protein HypF

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 753; Mature: 752

Protein sequence:

>753_residues
MPERIAQFITIGGRVQGVGFRPFVYRAARAHALAGWVRNANGSVEIHVEGERAQLQGFVDTLLNEAPPLSAPGPLAIRPC
ASTRLRGFSILDSTAASEADVHLPADGFVCADCLNELHDPANRRYRYPFINCTQCGPRYTLITGLPYDRPMTAMRGFTLC
AACRREYEDAGDRRFHAEPIACPVCGPHLQFVSGDKTLSGDETALAAAVAQLRAGKIVAVKGVGGYHLMCDARNDPAVAA
LRARKPRPAKPLAVMFRDLDSLRDAVHFDTDDAALLSSPARPIVLLRKRNTNPLSAAIAPGLGEIGCMLPYSPLHDLLLH
DFGGPLVASSGNLSGEPVLTANAEAQARLRHLADAFLHHDRPIVRPADDPVYRRIAGAPRPLRLGRGLAPLELELPAALA
QPVLALGSHMKNTVCLAWGTRAVVSPHIGELDSARSLDTLARVAADLQDLYQVRAATLLVDHHPGFGYRRFARDSGLPLA
AVWHHHAHASALAAEFEDISEWIVFAWDGVGLGEDMSLWGGEAFAGAPGRWRRAASWRPFRLPGGDKAGREPWRAAAALL
WETGAPAAFAPPALHQAWAARLNSPGSSSVGRLLDAAAALCGICTEASFEGEAPMRIEACASDIAPDGTCIDAAALTLPL
AADAQGVWRSDWAPLLPMLADATQPASRRAARFHLSLAEALVAQAKQLRGDTGIRTVGLTGGVFQNRLLTETAALALAAA
GFSVRLPQRVPMNDAGLSFGQVIEFLYRQHTKT

Sequences:

>Translated_753_residues
MPERIAQFITIGGRVQGVGFRPFVYRAARAHALAGWVRNANGSVEIHVEGERAQLQGFVDTLLNEAPPLSAPGPLAIRPC
ASTRLRGFSILDSTAASEADVHLPADGFVCADCLNELHDPANRRYRYPFINCTQCGPRYTLITGLPYDRPMTAMRGFTLC
AACRREYEDAGDRRFHAEPIACPVCGPHLQFVSGDKTLSGDETALAAAVAQLRAGKIVAVKGVGGYHLMCDARNDPAVAA
LRARKPRPAKPLAVMFRDLDSLRDAVHFDTDDAALLSSPARPIVLLRKRNTNPLSAAIAPGLGEIGCMLPYSPLHDLLLH
DFGGPLVASSGNLSGEPVLTANAEAQARLRHLADAFLHHDRPIVRPADDPVYRRIAGAPRPLRLGRGLAPLELELPAALA
QPVLALGSHMKNTVCLAWGTRAVVSPHIGELDSARSLDTLARVAADLQDLYQVRAATLLVDHHPGFGYRRFARDSGLPLA
AVWHHHAHASALAAEFEDISEWIVFAWDGVGLGEDMSLWGGEAFAGAPGRWRRAASWRPFRLPGGDKAGREPWRAAAALL
WETGAPAAFAPPALHQAWAARLNSPGSSSVGRLLDAAAALCGICTEASFEGEAPMRIEACASDIAPDGTCIDAAALTLPL
AADAQGVWRSDWAPLLPMLADATQPASRRAARFHLSLAEALVAQAKQLRGDTGIRTVGLTGGVFQNRLLTETAALALAAA
GFSVRLPQRVPMNDAGLSFGQVIEFLYRQHTKT
>Mature_752_residues
PERIAQFITIGGRVQGVGFRPFVYRAARAHALAGWVRNANGSVEIHVEGERAQLQGFVDTLLNEAPPLSAPGPLAIRPCA
STRLRGFSILDSTAASEADVHLPADGFVCADCLNELHDPANRRYRYPFINCTQCGPRYTLITGLPYDRPMTAMRGFTLCA
ACRREYEDAGDRRFHAEPIACPVCGPHLQFVSGDKTLSGDETALAAAVAQLRAGKIVAVKGVGGYHLMCDARNDPAVAAL
RARKPRPAKPLAVMFRDLDSLRDAVHFDTDDAALLSSPARPIVLLRKRNTNPLSAAIAPGLGEIGCMLPYSPLHDLLLHD
FGGPLVASSGNLSGEPVLTANAEAQARLRHLADAFLHHDRPIVRPADDPVYRRIAGAPRPLRLGRGLAPLELELPAALAQ
PVLALGSHMKNTVCLAWGTRAVVSPHIGELDSARSLDTLARVAADLQDLYQVRAATLLVDHHPGFGYRRFARDSGLPLAA
VWHHHAHASALAAEFEDISEWIVFAWDGVGLGEDMSLWGGEAFAGAPGRWRRAASWRPFRLPGGDKAGREPWRAAAALLW
ETGAPAAFAPPALHQAWAARLNSPGSSSVGRLLDAAAALCGICTEASFEGEAPMRIEACASDIAPDGTCIDAAALTLPLA
ADAQGVWRSDWAPLLPMLADATQPASRRAARFHLSLAEALVAQAKQLRGDTGIRTVGLTGGVFQNRLLTETAALALAAAG
FSVRLPQRVPMNDAGLSFGQVIEFLYRQHTKT

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=753, Percent_Identity=40.9030544488712, Blast_Score=429, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 80556; Mature: 80425

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPERIAQFITIGGRVQGVGFRPFVYRAARAHALAGWVRNANGSVEIHVEGERAQLQGFVD
CCHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHH
TLLNEAPPLSAPGPLAIRPCASTRLRGFSILDSTAASEADVHLPADGFVCADCLNELHDP
HHHCCCCCCCCCCCEEEECCCCCCCCCEEEHHCCCCCCCCEECCCCCCHHHHHHHHHCCC
ANRRYRYPFINCTQCGPRYTLITGLPYDRPMTAMRGFTLCAACRREYEDAGDRRFHAEPI
HHCCEECCEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCC
ACPVCGPHLQFVSGDKTLSGDETALAAAVAQLRAGKIVAVKGVGGYHLMCDARNDPAVAA
CCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCHHHH
LRARKPRPAKPLAVMFRDLDSLRDAVHFDTDDAALLSSPARPIVLLRKRNTNPLSAAIAP
HHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCEEEEEECCCCCCHHHHCC
GLGEIGCMLPYSPLHDLLLHDFGGPLVASSGNLSGEPVLTANAEAQARLRHLADAFLHHD
CCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHCC
RPIVRPADDPVYRRIAGAPRPLRLGRGLAPLELELPAALAQPVLALGSHMKNTVCLAWGT
CCCCCCCCCHHHHHHCCCCCCHHHCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEECC
RAVVSPHIGELDSARSLDTLARVAADLQDLYQVRAATLLVDHHPGFGYRRFARDSGLPLA
CHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHCCCCCCH
AVWHHHAHASALAAEFEDISEWIVFAWDGVGLGEDMSLWGGEAFAGAPGRWRRAASWRPF
HHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCE
RLPGGDKAGREPWRAAAALLWETGAPAAFAPPALHQAWAARLNSPGSSSVGRLLDAAAAL
ECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
CGICTEASFEGEAPMRIEACASDIAPDGTCIDAAALTLPLAADAQGVWRSDWAPLLPMLA
HHHHHCCCCCCCCCEEHHHHHHHCCCCCCCHHHHHHHEECCCCCCCCCCCCCHHHHHHHH
DATQPASRRAARFHLSLAEALVAQAKQLRGDTGIRTVGLTGGVFQNRLLTETAALALAAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHC
GFSVRLPQRVPMNDAGLSFGQVIEFLYRQHTKT
CCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PERIAQFITIGGRVQGVGFRPFVYRAARAHALAGWVRNANGSVEIHVEGERAQLQGFVD
CHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHH
TLLNEAPPLSAPGPLAIRPCASTRLRGFSILDSTAASEADVHLPADGFVCADCLNELHDP
HHHCCCCCCCCCCCEEEECCCCCCCCCEEEHHCCCCCCCCEECCCCCCHHHHHHHHHCCC
ANRRYRYPFINCTQCGPRYTLITGLPYDRPMTAMRGFTLCAACRREYEDAGDRRFHAEPI
HHCCEECCEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCC
ACPVCGPHLQFVSGDKTLSGDETALAAAVAQLRAGKIVAVKGVGGYHLMCDARNDPAVAA
CCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCHHHH
LRARKPRPAKPLAVMFRDLDSLRDAVHFDTDDAALLSSPARPIVLLRKRNTNPLSAAIAP
HHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCEEEEEECCCCCCHHHHCC
GLGEIGCMLPYSPLHDLLLHDFGGPLVASSGNLSGEPVLTANAEAQARLRHLADAFLHHD
CCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHCC
RPIVRPADDPVYRRIAGAPRPLRLGRGLAPLELELPAALAQPVLALGSHMKNTVCLAWGT
CCCCCCCCCHHHHHHCCCCCCHHHCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEECC
RAVVSPHIGELDSARSLDTLARVAADLQDLYQVRAATLLVDHHPGFGYRRFARDSGLPLA
CHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHCCCCCCH
AVWHHHAHASALAAEFEDISEWIVFAWDGVGLGEDMSLWGGEAFAGAPGRWRRAASWRPF
HHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCE
RLPGGDKAGREPWRAAAALLWETGAPAAFAPPALHQAWAARLNSPGSSSVGRLLDAAAAL
ECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
CGICTEASFEGEAPMRIEACASDIAPDGTCIDAAALTLPLAADAQGVWRSDWAPLLPMLA
HHHHHCCCCCCCCCEEHHHHHHHCCCCCCCHHHHHHHEECCCCCCCCCCCCCHHHHHHHH
DATQPASRRAARFHLSLAEALVAQAKQLRGDTGIRTVGLTGGVFQNRLLTETAALALAAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHC
GFSVRLPQRVPMNDAGLSFGQVIEFLYRQHTKT
CCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]