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The map label for this gene is ligT [H]

Identifier: 74317023

GI number: 74317023

Start: 1072649

End: 1073200

Strand: Direct

Name: ligT [H]

Synonym: Tbd_1005

Alternate gene names: 74317023

Gene position: 1072649-1073200 (Clockwise)

Preceding gene: 74317022

Following gene: 74317024

Centisome position: 36.86

GC content: 68.66

Gene sequence:

>552_bases
GTGGCTGACTCCCGCACGCCGGACGTCCCTTCCCTCCGCCTGTTCTTCGCCCTGTGGCCGGACGACGCCACCCGCGACGC
GCTCGACCGCACCGGCAAGTGGCTGCATCGGCACTGGGGCGGGCGGCGCATGCGTGCCGAGACCCTGCACATCACGCTCG
CCTTTCTCGGCACGACGCCCGCCGACAGGCTCGACGCGCTTATTGGCTGCGCCGACGGCATAGCCGCCGAGCCCTTCGCG
CTTGTGCTCGACCGGCCCGGCTACTGGCGGCATAACCATGTCGGCTGGCTCGGCGCGAGTGAGGCGCCGCCGCCTCATCT
CGCGCTCGTGACGGCGCTCAATGCCGCTCTCCAGGCGGGCGGTTTTCCGGTCGATGCACGTGCGCACGTGCCTCATGTCA
CGCTGTTGCGCAACACCGCCGGCGGCGACCTGCCCCTATGCAGGCCGGTAAGTTGGGCGGTCCCGGATTTCGTGCTCGTG
AGATCCCGTACCGATCCCACAGGTGCGCAGTACGAAGTGATTCGACGCTGGCTGCTGGTCCATCCTAAATGA

Upstream 100 bases:

>100_bases
GCGATCGACCGCGTCAACGTCAAGGCGACGACCACCGAAAGGCTCGGCTTCGCCGGCCGCGGCGAGGGCATCGCCGCCGA
GGCCGTATGCCTGATCGAGC

Downstream 100 bases:

>100_bases
GAGCCAGGGAACCGAGTCGGCGACGCTGGAAATGAGCTGTCTGGCCCTTTGCGTGCCCTGAAATGATCGGTTGATCGCCT
ACCTGTGTGCTCCGCACGCG

Product: 2',5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 183; Mature: 182

Protein sequence:

>183_residues
MADSRTPDVPSLRLFFALWPDDATRDALDRTGKWLHRHWGGRRMRAETLHITLAFLGTTPADRLDALIGCADGIAAEPFA
LVLDRPGYWRHNHVGWLGASEAPPPHLALVTALNAALQAGGFPVDARAHVPHVTLLRNTAGGDLPLCRPVSWAVPDFVLV
RSRTDPTGAQYEVIRRWLLVHPK

Sequences:

>Translated_183_residues
MADSRTPDVPSLRLFFALWPDDATRDALDRTGKWLHRHWGGRRMRAETLHITLAFLGTTPADRLDALIGCADGIAAEPFA
LVLDRPGYWRHNHVGWLGASEAPPPHLALVTALNAALQAGGFPVDARAHVPHVTLLRNTAGGDLPLCRPVSWAVPDFVLV
RSRTDPTGAQYEVIRRWLLVHPK
>Mature_182_residues
ADSRTPDVPSLRLFFALWPDDATRDALDRTGKWLHRHWGGRRMRAETLHITLAFLGTTPADRLDALIGCADGIAAEPFAL
VLDRPGYWRHNHVGWLGASEAPPPHLALVTALNAALQAGGFPVDARAHVPHVTLLRNTAGGDLPLCRPVSWAVPDFVLVR
SRTDPTGAQYEVIRRWLLVHPK

Specific function: Specifically ligates tRNA half-molecules containing nucleoside base modifications and shows a preference among different tRNA species. Acts in the absence of ATP to form a 2'-5' phosphodiester linkage [H]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081692, Length=174, Percent_Identity=32.183908045977, Blast_Score=71, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 20192; Mature: 20061

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADSRTPDVPSLRLFFALWPDDATRDALDRTGKWLHRHWGGRRMRAETLHITLAFLGTTP
CCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCC
ADRLDALIGCADGIAAEPFALVLDRPGYWRHNHVGWLGASEAPPPHLALVTALNAALQAG
HHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCC
GFPVDARAHVPHVTLLRNTAGGDLPLCRPVSWAVPDFVLVRSRTDPTGAQYEVIRRWLLV
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHC
HPK
CCC
>Mature Secondary Structure 
ADSRTPDVPSLRLFFALWPDDATRDALDRTGKWLHRHWGGRRMRAETLHITLAFLGTTP
CCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCC
ADRLDALIGCADGIAAEPFALVLDRPGYWRHNHVGWLGASEAPPPHLALVTALNAALQAG
HHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCC
GFPVDARAHVPHVTLLRNTAGGDLPLCRPVSWAVPDFVLVRSRTDPTGAQYEVIRRWLLV
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHC
HPK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 2013578; 8940112 [H]