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The map label for this gene is ispF

Identifier: 74317022

GI number: 74317022

Start: 1072174

End: 1072656

Strand: Direct

Name: ispF

Synonym: Tbd_1004

Alternate gene names: 74317022

Gene position: 1072174-1072656 (Clockwise)

Preceding gene: 74317021

Following gene: 74317023

Centisome position: 36.85

GC content: 68.32

Gene sequence:

>483_bases
ATGCGTGAGATGCGCGTCGGCCACGGTTACGACGTCCACGTGCTCGTCGAAAATCGCCGGCTCATCATCGGCGGGGTCGA
GATTCCCTACGAGCGCGGCCTCGCGGGACATTCGGACGCCGACGTGCTGCTGCACGCGATCTGCGACGCGCTCCTGGGTG
CGGTCGGGCTCGGCGACATCGGCCGGCATTTTCCGGACACCGACGCTGCGTTCGCCGGCATCGACAGCCGTATCCTGCTG
CGCCGCGTCGCGCAGCAATTGAAAGAGCGCGCCTGGCAAGTCGGCAACGTTGACGCGACCATCATCGCCCAGGCGCCGAA
GATGGCGCCGCACATCGCGCGCATGACCGCCCACATCGCCGACGATCTCGGCGTCGCGATCGACCGCGTCAACGTCAAGG
CGACGACCACCGAAAGGCTCGGCTTCGCCGGCCGCGGCGAGGGCATCGCCGCCGAGGCCGTATGCCTGATCGAGCGTGGC
TGA

Upstream 100 bases:

>100_bases
CTCGGGCTGCAGCCGCAGCTCGTCGAATCCGACAGCCGCAACCTCAAGGTCACCTATCCGCAGGACCTCGAACTCGCGAG
CCTGATCCTGGGGAAGATGA

Downstream 100 bases:

>100_bases
CTCCCGCACGCCGGACGTCCCTTCCCTCCGCCTGTTCTTCGCCCTGTGGCCGGACGACGCCACCCGCGACGCGCTCGACC
GCACCGGCAAGTGGCTGCAT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MREMRVGHGYDVHVLVENRRLIIGGVEIPYERGLAGHSDADVLLHAICDALLGAVGLGDIGRHFPDTDAAFAGIDSRILL
RRVAQQLKERAWQVGNVDATIIAQAPKMAPHIARMTAHIADDLGVAIDRVNVKATTTERLGFAGRGEGIAAEAVCLIERG

Sequences:

>Translated_160_residues
MREMRVGHGYDVHVLVENRRLIIGGVEIPYERGLAGHSDADVLLHAICDALLGAVGLGDIGRHFPDTDAAFAGIDSRILL
RRVAQQLKERAWQVGNVDATIIAQAPKMAPHIARMTAHIADDLGVAIDRVNVKATTTERLGFAGRGEGIAAEAVCLIERG
>Mature_160_residues
MREMRVGHGYDVHVLVENRRLIIGGVEIPYERGLAGHSDADVLLHAICDALLGAVGLGDIGRHFPDTDAAFAGIDSRILL
RRVAQQLKERAWQVGNVDATIIAQAPKMAPHIARMTAHIADDLGVAIDRVNVKATTTERLGFAGRGEGIAAEAVCLIERG

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=63.6363636363636, Blast_Score=190, Evalue=4e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_THIDA (Q3SK37)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314762.1
- ProteinModelPortal:   Q3SK37
- SMR:   Q3SK37
- STRING:   Q3SK37
- GeneID:   3671227
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1004
- NMPDR:   fig|292415.3.peg.734
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- PhylomeDB:   Q3SK37
- BioCyc:   TDEN292415:TBD_1004-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17176; Mature: 17176

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREMRVGHGYDVHVLVENRRLIIGGVEIPYERGLAGHSDADVLLHAICDALLGAVGLGDI
CCCCCCCCCCEEEEEEECCEEEEECCCCCHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHH
GRHFPDTDAAFAGIDSRILLRRVAQQLKERAWQVGNVDATIIAQAPKMAPHIARMTAHIA
HCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHH
DDLGVAIDRVNVKATTTERLGFAGRGEGIAAEAVCLIERG
HHHHHEEEEEEEEEECHHHCCCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MREMRVGHGYDVHVLVENRRLIIGGVEIPYERGLAGHSDADVLLHAICDALLGAVGLGDI
CCCCCCCCCCEEEEEEECCEEEEECCCCCHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHH
GRHFPDTDAAFAGIDSRILLRRVAQQLKERAWQVGNVDATIIAQAPKMAPHIARMTAHIA
HCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHH
DDLGVAIDRVNVKATTTERLGFAGRGEGIAAEAVCLIERG
HHHHHEEEEEEEEEECHHHCCCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA