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The map label for this gene is ispD

Identifier: 74317021

GI number: 74317021

Start: 1071480

End: 1072181

Strand: Direct

Name: ispD

Synonym: Tbd_1003

Alternate gene names: 74317021

Gene position: 1071480-1072181 (Clockwise)

Preceding gene: 74317020

Following gene: 74317022

Centisome position: 36.82

GC content: 67.09

Gene sequence:

>702_bases
ATGAAATTCTACGCACTGATACCCGCTGCCGGCTCGGGCTCACGCATGGGCGGCGCGATCGAAAAGCAGTATATGGATGT
CAACTCGGTGCCGATGATCGCGCACGCGATGATGGTGCTCGCGCGCGAGCCGCGCATCGCCCGAATTTTTGTTGTGCTCT
CGCCGACCGACAAGCGCTGGAACAACTACGAATGGCAGGGCTGGGAGGAGCGCATCGAAGTCCTGCGCTGTGGCGGCGGG
ACCCGCGCCGAAACCGTGCTGAACGCGCTCGACGCCATCGCCGAGGTCTGCGACCCCGCTGACTGGGTGCTCGTGCACGA
CGCCGCGCGGCCCTGTCTGCCCGACGAAATGCTCGGCAAGCTGCTCGACGAAGTTGCGGACGACCCGGTCGGCGGGTTGC
TTGCGGTGCCGGTCGCCGACACCCTCAAGCGCGCCGCTGGCGACACGTCATCGGGCACGCGGGTTGAGGCCACCGTGCCG
CGTGCCGGCCTGTGGCAGGCGCAGACCCCGCAGATGTTCCGCCACGGCACGCTGACCGAAGCGCTGCGCGCGGCGGGCAG
CGACATGACCGACGAGGCCTCGGCGATCGAGAAGCTCGGGCTGCAGCCGCAGCTCGTCGAATCCGACAGCCGCAACCTCA
AGGTCACCTATCCGCAGGACCTCGAACTCGCGAGCCTGATCCTGGGGAAGATGAATGCGTGA

Upstream 100 bases:

>100_bases
ACCGCGCCGCCCGCATGATCGAGGATATGGAACGCGCCGGGCTCGTCTCGTCGATGCAGTCGAACGGCAACCGGGAGGTG
CTGGCGCCCGGAGGTGAGGC

Downstream 100 bases:

>100_bases
GATGCGCGTCGGCCACGGTTACGACGTCCACGTGCTCGTCGAAAATCGCCGGCTCATCATCGGCGGGGTCGAGATTCCCT
ACGAGCGCGGCCTCGCGGGA

Product: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MKFYALIPAAGSGSRMGGAIEKQYMDVNSVPMIAHAMMVLAREPRIARIFVVLSPTDKRWNNYEWQGWEERIEVLRCGGG
TRAETVLNALDAIAEVCDPADWVLVHDAARPCLPDEMLGKLLDEVADDPVGGLLAVPVADTLKRAAGDTSSGTRVEATVP
RAGLWQAQTPQMFRHGTLTEALRAAGSDMTDEASAIEKLGLQPQLVESDSRNLKVTYPQDLELASLILGKMNA

Sequences:

>Translated_233_residues
MKFYALIPAAGSGSRMGGAIEKQYMDVNSVPMIAHAMMVLAREPRIARIFVVLSPTDKRWNNYEWQGWEERIEVLRCGGG
TRAETVLNALDAIAEVCDPADWVLVHDAARPCLPDEMLGKLLDEVADDPVGGLLAVPVADTLKRAAGDTSSGTRVEATVP
RAGLWQAQTPQMFRHGTLTEALRAAGSDMTDEASAIEKLGLQPQLVESDSRNLKVTYPQDLELASLILGKMNA
>Mature_233_residues
MKFYALIPAAGSGSRMGGAIEKQYMDVNSVPMIAHAMMVLAREPRIARIFVVLSPTDKRWNNYEWQGWEERIEVLRCGGG
TRAETVLNALDAIAEVCDPADWVLVHDAARPCLPDEMLGKLLDEVADDPVGGLLAVPVADTLKRAAGDTSSGTRVEATVP
RAGLWQAQTPQMFRHGTLTEALRAAGSDMTDEASAIEKLGLQPQLVESDSRNLKVTYPQDLELASLILGKMNA

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=235, Percent_Identity=25.531914893617, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1789104, Length=230, Percent_Identity=42.1739130434783, Blast_Score=159, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_THIDA (Q3SK38)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314761.1
- ProteinModelPortal:   Q3SK38
- SMR:   Q3SK38
- STRING:   Q3SK38
- GeneID:   3671226
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1003
- NMPDR:   fig|292415.3.peg.733
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   ALSIVHT
- PhylomeDB:   Q3SK38
- BioCyc:   TDEN292415:TBD_1003-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 25286; Mature: 25286

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFYALIPAAGSGSRMGGAIEKQYMDVNSVPMIAHAMMVLAREPRIARIFVVLSPTDKRW
CCEEEEEECCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCC
NNYEWQGWEERIEVLRCGGGTRAETVLNALDAIAEVCDPADWVLVHDAARPCLPDEMLGK
CCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHH
LLDEVADDPVGGLLAVPVADTLKRAAGDTSSGTRVEATVPRAGLWQAQTPQMFRHGTLTE
HHHHHCCCCCCCEEEECHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHCCCHHH
ALRAAGSDMTDEASAIEKLGLQPQLVESDSRNLKVTYPQDLELASLILGKMNA
HHHHCCCCCCHHHHHHHHHCCCCCEECCCCCCEEEECCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKFYALIPAAGSGSRMGGAIEKQYMDVNSVPMIAHAMMVLAREPRIARIFVVLSPTDKRW
CCEEEEEECCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCC
NNYEWQGWEERIEVLRCGGGTRAETVLNALDAIAEVCDPADWVLVHDAARPCLPDEMLGK
CCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHH
LLDEVADDPVGGLLAVPVADTLKRAAGDTSSGTRVEATVPRAGLWQAQTPQMFRHGTLTE
HHHHHCCCCCCCEEEECHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHCCCHHH
ALRAAGSDMTDEASAIEKLGLQPQLVESDSRNLKVTYPQDLELASLILGKMNA
HHHHCCCCCCHHHHHHHHHCCCCCEECCCCCCEEEECCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA