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The map label for this gene is pdhB [H]

Identifier: 74316672

GI number: 74316672

Start: 694675

End: 695658

Strand: Reverse

Name: pdhB [H]

Synonym: Tbd_0654

Alternate gene names: 74316672

Gene position: 695658-694675 (Counterclockwise)

Preceding gene: 74316673

Following gene: 74316671

Centisome position: 23.91

GC content: 64.33

Gene sequence:

>984_bases
ATGGCAAACATCATGTACTGGGAGGCGATCCAGCGCGCCCACGACGAAGAAATGGCGCGCGACCCCCTGGTCATCTGCCT
GGGCGAGGACATCGGCGTCGCCGGCGGCACCTACAAGGCGACCAAGGGCCTGTACGAAAAATACGGCCCGTTGCGCGTGA
TGGACACACCGATTTCCGAGGGCGGCTTCACGGGTCTCGCGGTCGGCGCTTCCTTCCTCGGCGTGCGGCCCATCGTCGAG
ATCATGTCGGTCAACTTCGCCTGGCTCGCGATGGACCAGATGTTCAACTCCGCCGCCAAGGTGCGTTACATGTCGGGCGG
CCAGCTGACGGCCCCGTGCGTGTTCCGTTCGGCGGGCGGCGCCGCCCACCAACTCGGCGCGCAGCACTCCGCGCGCATGG
AGAAGGTGTTCATGGGCATCGCCGGCCTGCGTGTCGTCACGCCGTCGAACCCGAAGCAGGCCTACGGCCTGCTGAAGTCG
GCGATCCGCTGCGACGACCCGGTGTTCATCAACGAACACGAACTCATGTACAACATGAAGGGCGAAGTCCCGGACGGCGA
ATACTTCCACCCGCTCGAAGGGTCGGAAGTCGCACGCGCCGGCACCGACGTCACGCTCTTCGGCTATAACATTTCTGTGC
ACTGGTGCCTGAAAGCCGCCGAAATCCTCGACAAGCAGTACGGCATCTCGGCCGAGGTCGTCGACCTTTATTCGCTCGCC
CCCCTCGATCGCGCCGGCATCAAGGCTTCGGTCACGAAGACGCATCGTGCCGTCGTCGTCGAGGAAGACGAAGCGCCGGT
CGGCGTCGGCTCCGAAGTGATCGCGATCATCAACGAGGAGTGCTTCTTCGAGCTCGACGCCGCGCCGGTGCGCGTGCACT
CGGCGCTGGTGCCGCAACCGTACAACCACACGCTGGAAAAAGCAGCGATCCCGGATCACGAAGACGTCGTGAAGGCGGTC
CTGAAGATGTTCGGGCGCGCCTGA

Upstream 100 bases:

>100_bases
TACTCGCCGATCGCGAGAGCCAGCTGCCCTGGCTCACCGGCAAGGCCGCCTGAGGCCGGCGCCGCGTTCACGCGACACTG
AATCAGAAGGAATAAACAGC

Downstream 100 bases:

>100_bases
CGCGCGCAGGGGTCAGGGGTCGGGATTCAGGGGTCAGGGATGCAAAACTACCGTGACCTCAAGGTCTGGCAGTCGGCAAT
GGAACTCGCCGAAAGCGTGT

Product: pyruvate dehydrogenase E1 subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MANIMYWEAIQRAHDEEMARDPLVICLGEDIGVAGGTYKATKGLYEKYGPLRVMDTPISEGGFTGLAVGASFLGVRPIVE
IMSVNFAWLAMDQMFNSAAKVRYMSGGQLTAPCVFRSAGGAAHQLGAQHSARMEKVFMGIAGLRVVTPSNPKQAYGLLKS
AIRCDDPVFINEHELMYNMKGEVPDGEYFHPLEGSEVARAGTDVTLFGYNISVHWCLKAAEILDKQYGISAEVVDLYSLA
PLDRAGIKASVTKTHRAVVVEEDEAPVGVGSEVIAIINEECFFELDAAPVRVHSALVPQPYNHTLEKAAIPDHEDVVKAV
LKMFGRA

Sequences:

>Translated_327_residues
MANIMYWEAIQRAHDEEMARDPLVICLGEDIGVAGGTYKATKGLYEKYGPLRVMDTPISEGGFTGLAVGASFLGVRPIVE
IMSVNFAWLAMDQMFNSAAKVRYMSGGQLTAPCVFRSAGGAAHQLGAQHSARMEKVFMGIAGLRVVTPSNPKQAYGLLKS
AIRCDDPVFINEHELMYNMKGEVPDGEYFHPLEGSEVARAGTDVTLFGYNISVHWCLKAAEILDKQYGISAEVVDLYSLA
PLDRAGIKASVTKTHRAVVVEEDEAPVGVGSEVIAIINEECFFELDAAPVRVHSALVPQPYNHTLEKAAIPDHEDVVKAV
LKMFGRA
>Mature_326_residues
ANIMYWEAIQRAHDEEMARDPLVICLGEDIGVAGGTYKATKGLYEKYGPLRVMDTPISEGGFTGLAVGASFLGVRPIVEI
MSVNFAWLAMDQMFNSAAKVRYMSGGQLTAPCVFRSAGGAAHQLGAQHSARMEKVFMGIAGLRVVTPSNPKQAYGLLKSA
IRCDDPVFINEHELMYNMKGEVPDGEYFHPLEGSEVARAGTDVTLFGYNISVHWCLKAAEILDKQYGISAEVVDLYSLAP
LDRAGIKASVTKTHRAVVVEEDEAPVGVGSEVIAIINEECFFELDAAPVRVHSALVPQPYNHTLEKAAIPDHEDVVKAVL
KMFGRA

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=322, Percent_Identity=43.167701863354, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI291084858, Length=322, Percent_Identity=40.9937888198758, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI4557353, Length=323, Percent_Identity=35.9133126934984, Blast_Score=185, Evalue=4e-47,
Organism=Homo sapiens, GI34101272, Length=323, Percent_Identity=35.9133126934984, Blast_Score=185, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17538422, Length=320, Percent_Identity=45, Blast_Score=257, Evalue=5e-69,
Organism=Caenorhabditis elegans, GI17506935, Length=322, Percent_Identity=33.2298136645963, Blast_Score=155, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6319698, Length=318, Percent_Identity=44.9685534591195, Blast_Score=256, Evalue=2e-69,
Organism=Drosophila melanogaster, GI21358145, Length=323, Percent_Identity=45.2012383900929, Blast_Score=258, Evalue=5e-69,
Organism=Drosophila melanogaster, GI24650940, Length=323, Percent_Identity=45.2012383900929, Blast_Score=258, Evalue=5e-69,
Organism=Drosophila melanogaster, GI160714828, Length=316, Percent_Identity=35.4430379746835, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI160714832, Length=316, Percent_Identity=35.4430379746835, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24650943, Length=85, Percent_Identity=49.4117647058824, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24650945, Length=85, Percent_Identity=49.4117647058824, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 35535; Mature: 35404

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANIMYWEAIQRAHDEEMARDPLVICLGEDIGVAGGTYKATKGLYEKYGPLRVMDTPISE
CCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCC
GGFTGLAVGASFLGVRPIVEIMSVNFAWLAMDQMFNSAAKVRYMSGGQLTAPCVFRSAGG
CCCCHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHEEEEECCCCEECCEEEECCCC
AAHQLGAQHSARMEKVFMGIAGLRVVTPSNPKQAYGLLKSAIRCDDPVFINEHELMYNMK
HHHHHCCHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHCCCCCEEECCCEEEEEEC
GEVPDGEYFHPLEGSEVARAGTDVTLFGYNISVHWCLKAAEILDKQYGISAEVVDLYSLA
CCCCCCCEECCCCCCHHHHCCCCEEEEEEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHCC
PLDRAGIKASVTKTHRAVVVEEDEAPVGVGSEVIAIINEECFFELDAAPVRVHSALVPQP
CCCCCCCEEEEECCEEEEEEECCCCCCCCCCCEEEEECCCCEEEECCCCEEEHHHHCCCC
YNHTLEKAAIPDHEDVVKAVLKMFGRA
CCCHHHHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ANIMYWEAIQRAHDEEMARDPLVICLGEDIGVAGGTYKATKGLYEKYGPLRVMDTPISE
CCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCC
GGFTGLAVGASFLGVRPIVEIMSVNFAWLAMDQMFNSAAKVRYMSGGQLTAPCVFRSAGG
CCCCHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHEEEEECCCCEECCEEEECCCC
AAHQLGAQHSARMEKVFMGIAGLRVVTPSNPKQAYGLLKSAIRCDDPVFINEHELMYNMK
HHHHHCCHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHCCCCCEEECCCEEEEEEC
GEVPDGEYFHPLEGSEVARAGTDVTLFGYNISVHWCLKAAEILDKQYGISAEVVDLYSLA
CCCCCCCEECCCCCCHHHHCCCCEEEEEEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHCC
PLDRAGIKASVTKTHRAVVVEEDEAPVGVGSEVIAIINEECFFELDAAPVRVHSALVPQP
CCCCCCCEEEEECCEEEEEEECCCCCCCCCCCEEEEECCCCEEEECCCCEEEHHHHCCCC
YNHTLEKAAIPDHEDVVKAVLKMFGRA
CCCHHHHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]