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The map label for this gene is ligA

Identifier: 74316642

GI number: 74316642

Start: 655939

End: 657999

Strand: Direct

Name: ligA

Synonym: Tbd_0624

Alternate gene names: 74316642

Gene position: 655939-657999 (Clockwise)

Preceding gene: 74316641

Following gene: 74316643

Centisome position: 22.54

GC content: 69.67

Gene sequence:

>2061_bases
ATGGCCGACCGCGATGTGCAGGCGCGCGCCGCCGCGCTGCGCCGCGAGATCGAGCGCCACAACTACGCCTACTATGTCCT
CGACGCGCCGACCATTCCCGACGCCGAGTACGACCGGCTCTACCATGAACTCGAAGCGCTCGAAGCGGCGCATCCCGAGC
TTGTCGCCGCCGACTCGCCGACGCGCCGCGTCGGCGGCCAGCCGCTGGCCGGCTTCAAGCCGGTGCGGCACGCGGTGCCG
ATGCTGTCGATCCGTACCGAGACCGACACCACGGCTGCCGGGGCGCGTGCCTTCGACGTGCGGGTGCGCAAGGAACTCGG
CCTCGCGCCTGCCGACCCGCCCGTCGAATACGCCGCAGAACTGAAATTCGACGGCCTCGCCATCAACCTGCGCTACGAGG
CGGGCGTGCTCGTCGCGGCCGCGACGCGCGGCGACGGCGAAACCGGCGAGGACGTCACGCAGAACGTGCGCACGGTGCGC
ACGATTCCCCTGCGCCTGCACGGCGACGCACCGCCTGCGGTACTCGAGGTGCGCGGCGAGGTTTTCATGCGCCGCGACGA
CTTCGAACGCTATAACGCGCAGCAGCGCGCCGCCGGCAAGCCGACGCTCGTCAACCCCAGGAACGGCGCGGCGGGCAGCA
TCCGTCAACTCGATCCGGCGATCGCGGGTCAGCGGCCGCTGTCCTTCTACGCCTACGGGCTCGGCGAGGTCGTCGGCTGG
CCCGATGCCCCGGCGACGCACGGCGCGACGCTCGACGCGCTGCAGGGGCTCGGGGTTCCGGTCAGCGCCGAGCGCTGCGT
CTGCAACGGGGTCGACGACCTGATCGCTTTCCACGACGACATCGCCGCGCGTCGAGACCAGTTGCCGTTCGACATTGACG
GCGTCGTCTACAAGGTGAACGCGTTCGCCTTGCAGCGCGAACTCGGTTTTCTGACGCGCGAGCCGCGCTGGGCGGTGGCG
CACAAGTATCCGGCGCAGGAACAGCTCACCACGGTCCTCGGCATCGGCGTCCACGTCGGCCGCACCGGCGCGCTCACGCC
GGTCGCCCGGCTCGCGCCGGTGTTCGTCGGCGGGGTCACGGTCACCAACGCGACGCTGCACAACCAGGACGAAATCGACC
GCAAGGACACGCGCGTCGGCGACACCGTGATCGTGCGCCGCGCGGGCGACGTCATCCCCGAAATCGTCGCGACCGTGCTC
GAGCGCCGGCCGCAGCCCGAGCCGCCTCGCTTCAACATCCTGCAGAGCTATCCGGTCTGCCCCGAGTGCGGCTCGCACGT
CGTCCGGCTCGAGGGCGAAGCCGTCGCGCGCTGCACCGGCGGCCTCTACTGCCCGGCCCAGCGCAAGCAGGCGCTGCTGC
ATTTCGCCAGCCGGCGGGCGATGGACATCGACGGCGTCGGCGAGAAGCTCGTCGACCAGCTCGTCTCCCGCGAACTCGTG
CGCACGCCGGCCGATCTCTACCGGCTCCCGCTCGAGACGCTCGCCGGGCTCGAGCGCATGGCCGAGAAATCGGCGCGCAA
CCTGATCCAAGCGATCGAGACGAGCAAGTCGACGACGCTCGCGCGCTTCATCTACGCGCTCGGCATACGCAACGTCGGCG
AAACGACGGCGCGCGACCTCGCGCGCCATTTCGGCGCGCTCGATGCGCTGCTCGGCGCGAACGAGACCGCTCTCCTGGGC
GTGCGCGACGTCGGCCCCATCGTCGCGCAGTCGATCGCGCAGTTTTTCGCCGAACCGCACAATCTCGAGGTCGTCGGCAA
GCTGCGCGCGCTCGGCGTGCACTGGCCCGAAACAACGGGTATGCAACAATCAGGGGGTATCCTCTCCGGCAAGACCCTCG
TATTGACCGGAACGCTGCCGGCGCTGACGCGCGATCAGGCCAAGGAGCGGATCGAGTCGGCCGGCGGCAAGGTGGCCGGC
TCGGTGTCGAAGAAGACCGACTACGTCGTCGCCGGCGAGGAAGCAGGCAGCAAGCTCGCGAAGGCCCGGGAACTCGGCGT
CACGATCCTCGACGAAGAGGAGCTGTTGGCGCTGCTCGGATCGAATAAAAAGAACGGATAG

Upstream 100 bases:

>100_bases
GTATCGAAAAAATCCGCAGCCAGCTCACCGAGATTTACGCGCGCATGGAGGCGCGCGGCGTCCCTTCCGGGTCCCGGCGT
TCGCTGCGTCTGTTCTCCTG

Downstream 100 bases:

>100_bases
CGCGGGGATTTTTGAACCAAGACCTTACCTGGGAAGAGACATGCAACAAGTCAGAAAAGCAGTATTTCCGGTTGCCGGCC
TCGGCACCCGTTTTTTGCCG

Product: NAD-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 686; Mature: 685

Protein sequence:

>686_residues
MADRDVQARAAALRREIERHNYAYYVLDAPTIPDAEYDRLYHELEALEAAHPELVAADSPTRRVGGQPLAGFKPVRHAVP
MLSIRTETDTTAAGARAFDVRVRKELGLAPADPPVEYAAELKFDGLAINLRYEAGVLVAAATRGDGETGEDVTQNVRTVR
TIPLRLHGDAPPAVLEVRGEVFMRRDDFERYNAQQRAAGKPTLVNPRNGAAGSIRQLDPAIAGQRPLSFYAYGLGEVVGW
PDAPATHGATLDALQGLGVPVSAERCVCNGVDDLIAFHDDIAARRDQLPFDIDGVVYKVNAFALQRELGFLTREPRWAVA
HKYPAQEQLTTVLGIGVHVGRTGALTPVARLAPVFVGGVTVTNATLHNQDEIDRKDTRVGDTVIVRRAGDVIPEIVATVL
ERRPQPEPPRFNILQSYPVCPECGSHVVRLEGEAVARCTGGLYCPAQRKQALLHFASRRAMDIDGVGEKLVDQLVSRELV
RTPADLYRLPLETLAGLERMAEKSARNLIQAIETSKSTTLARFIYALGIRNVGETTARDLARHFGALDALLGANETALLG
VRDVGPIVAQSIAQFFAEPHNLEVVGKLRALGVHWPETTGMQQSGGILSGKTLVLTGTLPALTRDQAKERIESAGGKVAG
SVSKKTDYVVAGEEAGSKLAKARELGVTILDEEELLALLGSNKKNG

Sequences:

>Translated_686_residues
MADRDVQARAAALRREIERHNYAYYVLDAPTIPDAEYDRLYHELEALEAAHPELVAADSPTRRVGGQPLAGFKPVRHAVP
MLSIRTETDTTAAGARAFDVRVRKELGLAPADPPVEYAAELKFDGLAINLRYEAGVLVAAATRGDGETGEDVTQNVRTVR
TIPLRLHGDAPPAVLEVRGEVFMRRDDFERYNAQQRAAGKPTLVNPRNGAAGSIRQLDPAIAGQRPLSFYAYGLGEVVGW
PDAPATHGATLDALQGLGVPVSAERCVCNGVDDLIAFHDDIAARRDQLPFDIDGVVYKVNAFALQRELGFLTREPRWAVA
HKYPAQEQLTTVLGIGVHVGRTGALTPVARLAPVFVGGVTVTNATLHNQDEIDRKDTRVGDTVIVRRAGDVIPEIVATVL
ERRPQPEPPRFNILQSYPVCPECGSHVVRLEGEAVARCTGGLYCPAQRKQALLHFASRRAMDIDGVGEKLVDQLVSRELV
RTPADLYRLPLETLAGLERMAEKSARNLIQAIETSKSTTLARFIYALGIRNVGETTARDLARHFGALDALLGANETALLG
VRDVGPIVAQSIAQFFAEPHNLEVVGKLRALGVHWPETTGMQQSGGILSGKTLVLTGTLPALTRDQAKERIESAGGKVAG
SVSKKTDYVVAGEEAGSKLAKARELGVTILDEEELLALLGSNKKNG
>Mature_685_residues
ADRDVQARAAALRREIERHNYAYYVLDAPTIPDAEYDRLYHELEALEAAHPELVAADSPTRRVGGQPLAGFKPVRHAVPM
LSIRTETDTTAAGARAFDVRVRKELGLAPADPPVEYAAELKFDGLAINLRYEAGVLVAAATRGDGETGEDVTQNVRTVRT
IPLRLHGDAPPAVLEVRGEVFMRRDDFERYNAQQRAAGKPTLVNPRNGAAGSIRQLDPAIAGQRPLSFYAYGLGEVVGWP
DAPATHGATLDALQGLGVPVSAERCVCNGVDDLIAFHDDIAARRDQLPFDIDGVVYKVNAFALQRELGFLTREPRWAVAH
KYPAQEQLTTVLGIGVHVGRTGALTPVARLAPVFVGGVTVTNATLHNQDEIDRKDTRVGDTVIVRRAGDVIPEIVATVLE
RRPQPEPPRFNILQSYPVCPECGSHVVRLEGEAVARCTGGLYCPAQRKQALLHFASRRAMDIDGVGEKLVDQLVSRELVR
TPADLYRLPLETLAGLERMAEKSARNLIQAIETSKSTTLARFIYALGIRNVGETTARDLARHFGALDALLGANETALLGV
RDVGPIVAQSIAQFFAEPHNLEVVGKLRALGVHWPETTGMQQSGGILSGKTLVLTGTLPALTRDQAKERIESAGGKVAGS
VSKKTDYVVAGEEAGSKLAKARELGVTILDEEELLALLGSNKKNG

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=75, Percent_Identity=50.6666666666667, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1788750, Length=678, Percent_Identity=54.2772861356932, Blast_Score=711, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=535, Percent_Identity=25.607476635514, Blast_Score=124, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_THIDA (Q3SL40)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314382.1
- ProteinModelPortal:   Q3SL40
- STRING:   Q3SL40
- GeneID:   3673735
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0624
- NMPDR:   fig|292415.3.peg.511
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q3SL40
- BioCyc:   TDEN292415:TBD_0624-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 74112; Mature: 73981

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 122-122 BINDING 120-120 BINDING 143-143 BINDING 180-180 BINDING 298-298 BINDING 322-322

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRDVQARAAALRREIERHNYAYYVLDAPTIPDAEYDRLYHELEALEAAHPELVAADSP
CCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEECCCC
TRRVGGQPLAGFKPVRHAVPMLSIRTETDTTAAGARAFDVRVRKELGLAPADPPVEYAAE
HHHCCCCCCCCCCHHHHHCCEEEEECCCCCCCCCCCCEEEEEHHHCCCCCCCCCHHHHHH
LKFDGLAINLRYEAGVLVAAATRGDGETGEDVTQNVRTVRTIPLRLHGDAPPAVLEVRGE
EEECCEEEEEEEECCEEEEEEECCCCCCCHHHHHCCCEEEEEEEEEECCCCCCEEEECCH
VFMRRDDFERYNAQQRAAGKPTLVNPRNGAAGSIRQLDPAIAGQRPLSFYAYGLGEVVGW
HEEECCHHHHHCCHHHCCCCCCEECCCCCCCCCHHHCCHHHCCCCCCHHHHHHHHHHCCC
PDAPATHGATLDALQGLGVPVSAERCVCNGVDDLIAFHDDIAARRDQLPFDIDGVVYKVN
CCCCCCCCCHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEH
AFALQRELGFLTREPRWAVAHKYPAQEQLTTVLGIGVHVGRTGALTPVARLAPVFVGGVT
HHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCEEECCCCCCCHHHHHHHHHCCCEE
VTNATLHNQDEIDRKDTRVGDTVIVRRAGDVIPEIVATVLERRPQPEPPRFNILQSYPVC
EEECEECCCHHCCHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCC
PECGSHVVRLEGEAVARCTGGLYCPAQRKQALLHFASRRAMDIDGVGEKLVDQLVSRELV
CCCCCCEEEECCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
RTPADLYRLPLETLAGLERMAEKSARNLIQAIETSKSTTLARFIYALGIRNVGETTARDL
HCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHH
ARHFGALDALLGANETALLGVRDVGPIVAQSIAQFFAEPHNLEVVGKLRALGVHWPETTG
HHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHCCCCCCCCC
MQQSGGILSGKTLVLTGTLPALTRDQAKERIESAGGKVAGSVSKKTDYVVAGEEAGSKLA
CCCCCCEECCCEEEEECCCCHHHHHHHHHHHHHCCCCEEECCCCCCCEEEECCHHHHHHH
KARELGVTILDEEELLALLGSNKKNG
HHHHCCCEEECHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ADRDVQARAAALRREIERHNYAYYVLDAPTIPDAEYDRLYHELEALEAAHPELVAADSP
CCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEECCCC
TRRVGGQPLAGFKPVRHAVPMLSIRTETDTTAAGARAFDVRVRKELGLAPADPPVEYAAE
HHHCCCCCCCCCCHHHHHCCEEEEECCCCCCCCCCCCEEEEEHHHCCCCCCCCCHHHHHH
LKFDGLAINLRYEAGVLVAAATRGDGETGEDVTQNVRTVRTIPLRLHGDAPPAVLEVRGE
EEECCEEEEEEEECCEEEEEEECCCCCCCHHHHHCCCEEEEEEEEEECCCCCCEEEECCH
VFMRRDDFERYNAQQRAAGKPTLVNPRNGAAGSIRQLDPAIAGQRPLSFYAYGLGEVVGW
HEEECCHHHHHCCHHHCCCCCCEECCCCCCCCCHHHCCHHHCCCCCCHHHHHHHHHHCCC
PDAPATHGATLDALQGLGVPVSAERCVCNGVDDLIAFHDDIAARRDQLPFDIDGVVYKVN
CCCCCCCCCHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEH
AFALQRELGFLTREPRWAVAHKYPAQEQLTTVLGIGVHVGRTGALTPVARLAPVFVGGVT
HHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCEEECCCCCCCHHHHHHHHHCCCEE
VTNATLHNQDEIDRKDTRVGDTVIVRRAGDVIPEIVATVLERRPQPEPPRFNILQSYPVC
EEECEECCCHHCCHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCC
PECGSHVVRLEGEAVARCTGGLYCPAQRKQALLHFASRRAMDIDGVGEKLVDQLVSRELV
CCCCCCEEEECCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
RTPADLYRLPLETLAGLERMAEKSARNLIQAIETSKSTTLARFIYALGIRNVGETTARDL
HCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHH
ARHFGALDALLGANETALLGVRDVGPIVAQSIAQFFAEPHNLEVVGKLRALGVHWPETTG
HHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHCCCCCCCCC
MQQSGGILSGKTLVLTGTLPALTRDQAKERIESAGGKVAGSVSKKTDYVVAGEEAGSKLA
CCCCCCEECCCEEEEECCCCHHHHHHHHHHHHHCCCCEEECCCCCCCEEEECCHHHHHHH
KARELGVTILDEEELLALLGSNKKNG
HHHHCCCEEECHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA