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The map label for this gene is ppsA [H]

Identifier: 74316636

GI number: 74316636

Start: 648183

End: 650522

Strand: Direct

Name: ppsA [H]

Synonym: Tbd_0618

Alternate gene names: 74316636

Gene position: 648183-650522 (Clockwise)

Preceding gene: 74316633

Following gene: 74316637

Centisome position: 22.28

GC content: 69.23

Gene sequence:

>2340_bases
ATGTCTCGTGACTATATTGTGCCGCTGGATGCGCTGTCGATGGCGGACGTGCCGCGCGTCGGCGGCAAGAACGCGTCGCT
CGGGGAAATGATTTCGCGGCTCGCCGATGCCGGGGTCAAGGTGCCGGGCGGCTTCGCGACGACCGCGCAGGCATTCTGGG
ACTTCCTGGATCACAACGGCCTGCGCGCACGGATCGGCGCCGTTCTGGACGCGCTCGACGTCACGGACGTAACGGCGCTC
GCCGCCGCCGGCGCCGAAATCCGCGCTGCGGTCGAAGGCGGGCGTCTGCCGCCGGCGCTGGAAAGCGGGTTGCGCGAGGC
CTACGCGGCGCTCGGCGACGACGTATCGGTCGCGGTGCGTTCGTCGGCCACTGCCGAAGACCTGCCCGACGCGTCCTTCG
CCGGGCAGCAGGAGACCTATCTGCACGTCCGCGGCGCCGAGGCCCTGCTCGCGCACCTGAAGAAGGTCTTCGCCTCGCTC
TACAACGACCGCGCGATCGCCTACCGTGTCCATCACGGCTTTGCGCACGCCGACGTCGCGCTCTCCGCCGGTGTGCAGCG
CATGGTGCGCGCGGACACCGGCTGTTCGGGCGTGCTGTTTACGCTCGATACCGAATCGGGCTTCCGCGACGTCGTCTTCA
TCACGGCGGCCTACGGTCTCGGCGAAACCGTTGTGCAGGGCGCGGTCAACCCCGACGAGTTCTACGTCCACAAGCCGACG
CTGGCGCTTGGTTTTCCGGCGCTCGTGCGGCGCGAGCGCGGCGAGAAGGCGATCCGCATGGTCTGGCGCGACGGCAAGAC
GGTGATCGAGGACACACCGCCCGAACTTCGGCAACGGTTCGCGCTGAGCGACGCCGAGGTGCTGGAACTCGCGCGCCAGG
CCGTGCAGATCGAGCGCCACTACGGGCGGCCGATGGACGTCGAGTGGGCCAAGGACGGCGTGAGCGGCGAGTTGTTCATC
GTCCAGGCGCGTCCCGAGACGGTCAAGGCGCGCGCCGCCGCCGGCGGCGAGCGCTACGTGCTCGGAGGGACCGGCGAGGT
GCGCGTCAGTGGCCGCGCGATCGGTCAGAAGATCGGTGCGGGGCCGGTGCGGGTCATCGCGACCCCGGCCGAGATGAACC
GCGTGCAGCGCGGCGACGTGCTCGTGACCGACATGACCGACCCCGACTGGGAACCGGTCATGAAGCGCGCCGCGGCGATC
GTCACCAACCGCGGCGGGCGCACCTGCCACGCCGCCATCGTCGCGCGCGAACTCGGCATTCCTGCCGTCGTCGGCGCGGG
CGACGCGACCACGGTGCTCAGGGATGACGAAGCGGTCACGGTCTCGTGCGCCGAGGGCGACACCGGGTTCGTCTACGCGG
GCAGGTTGCCGTTCGAGGTCGAGACGCTGGCGAGCGAGTCGCTGCCCACGCCGCCGGTCAAGCTCATGATGAACGTCGGC
AACCCCGAGCGCGCCTTCGAGTTCGCGCAGATCCCGAATCACGGCGTCGGCCTCGCACGGCTCGAATTCATCATTGCGCG
CATGATCGGCGTCCATCCGCTGGCCTTGCTCGACCATGCCGGGCAGCCGCAGGGCGTGCGCGACGAGATCGCTGCGCGCG
CCGCAGGCTACGCGGACCCGGTCGCGTTCTACGTCGACAAGCTCGCCGAAGGCATCGCGACGCTCGCCGCCGCCTTCTGG
CCGCAGCCCGTGATCGTGCGCCTCTCGGACTTCAAGTCCAACGAATACGCCAATCTGGTCGGCGGCGAGCGCTACGAGCC
GAAGGAGGAGAACCCGATGCTGGGCCTGCGCGGCGCGGCGCGCTACGTCTCGACATTGTTCCGCCCGGCGTTCGAGCTCG
AGTGCCGCGCGCTGAAAAAGGTCCGCGAGACGCTCGGTCTCGGCAACGTCGAGGTCATGATCCCGTTCGTGCGCACGGTC
GAGGAATGCGCCGCCGTCGTCGCGCTGCTCGCCGCGAACGGCCTCAGGCGGGGCGAACACGGCCTGCGTCTGATCATGAT
GTGCGAGATCCCGTCCAACGTGCTGCTCGCCGACGATTTTCTGCGTCACGTCGACGGTTTCTCGATCGGCAGCAACGACC
TGACCCAGCTGACGCTCGGCATCGACCGCGACTCGGGCCTCGTCGCGGAAGGCTTCGACGAGCGCAACCCGGCGGTCAGG
AAGCTGCTCGCCGAGGGGATCGCGGCGTGTCGGCGCAACGGCAAATACATCGGCATCTGCGGGCAGGGGCCGAGCGACCA
CCCCGACCTCGCGCAATGGCTCGTCGAACAGGGGATCGGGTCGATTTCGCTTAATCCCGACACGGTCGTCGCGACCTGGA
AACGGCTCGCGCACGGCTGA

Upstream 100 bases:

>100_bases
CGTGGGAACTGCGCCAGCACACTCTTGGCGACCGCCTCGATCGTGAGTCCCGTGTGATCGGACACGCAAAATACGCTGTA
GCCATTCATGGAGTTCACCT

Downstream 100 bases:

>100_bases
TGCGGGGGCGGCCATCGCGGGCGGTACGCTGCCCGCGCTGTCATTGAGCGCTCATCGGGGATGAAGTAAAATCCGCCATC
ACCCGCCCCCGCCCAGAAAA

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 779; Mature: 778

Protein sequence:

>779_residues
MSRDYIVPLDALSMADVPRVGGKNASLGEMISRLADAGVKVPGGFATTAQAFWDFLDHNGLRARIGAVLDALDVTDVTAL
AAAGAEIRAAVEGGRLPPALESGLREAYAALGDDVSVAVRSSATAEDLPDASFAGQQETYLHVRGAEALLAHLKKVFASL
YNDRAIAYRVHHGFAHADVALSAGVQRMVRADTGCSGVLFTLDTESGFRDVVFITAAYGLGETVVQGAVNPDEFYVHKPT
LALGFPALVRRERGEKAIRMVWRDGKTVIEDTPPELRQRFALSDAEVLELARQAVQIERHYGRPMDVEWAKDGVSGELFI
VQARPETVKARAAAGGERYVLGGTGEVRVSGRAIGQKIGAGPVRVIATPAEMNRVQRGDVLVTDMTDPDWEPVMKRAAAI
VTNRGGRTCHAAIVARELGIPAVVGAGDATTVLRDDEAVTVSCAEGDTGFVYAGRLPFEVETLASESLPTPPVKLMMNVG
NPERAFEFAQIPNHGVGLARLEFIIARMIGVHPLALLDHAGQPQGVRDEIAARAAGYADPVAFYVDKLAEGIATLAAAFW
PQPVIVRLSDFKSNEYANLVGGERYEPKEENPMLGLRGAARYVSTLFRPAFELECRALKKVRETLGLGNVEVMIPFVRTV
EECAAVVALLAANGLRRGEHGLRLIMMCEIPSNVLLADDFLRHVDGFSIGSNDLTQLTLGIDRDSGLVAEGFDERNPAVR
KLLAEGIAACRRNGKYIGICGQGPSDHPDLAQWLVEQGIGSISLNPDTVVATWKRLAHG

Sequences:

>Translated_779_residues
MSRDYIVPLDALSMADVPRVGGKNASLGEMISRLADAGVKVPGGFATTAQAFWDFLDHNGLRARIGAVLDALDVTDVTAL
AAAGAEIRAAVEGGRLPPALESGLREAYAALGDDVSVAVRSSATAEDLPDASFAGQQETYLHVRGAEALLAHLKKVFASL
YNDRAIAYRVHHGFAHADVALSAGVQRMVRADTGCSGVLFTLDTESGFRDVVFITAAYGLGETVVQGAVNPDEFYVHKPT
LALGFPALVRRERGEKAIRMVWRDGKTVIEDTPPELRQRFALSDAEVLELARQAVQIERHYGRPMDVEWAKDGVSGELFI
VQARPETVKARAAAGGERYVLGGTGEVRVSGRAIGQKIGAGPVRVIATPAEMNRVQRGDVLVTDMTDPDWEPVMKRAAAI
VTNRGGRTCHAAIVARELGIPAVVGAGDATTVLRDDEAVTVSCAEGDTGFVYAGRLPFEVETLASESLPTPPVKLMMNVG
NPERAFEFAQIPNHGVGLARLEFIIARMIGVHPLALLDHAGQPQGVRDEIAARAAGYADPVAFYVDKLAEGIATLAAAFW
PQPVIVRLSDFKSNEYANLVGGERYEPKEENPMLGLRGAARYVSTLFRPAFELECRALKKVRETLGLGNVEVMIPFVRTV
EECAAVVALLAANGLRRGEHGLRLIMMCEIPSNVLLADDFLRHVDGFSIGSNDLTQLTLGIDRDSGLVAEGFDERNPAVR
KLLAEGIAACRRNGKYIGICGQGPSDHPDLAQWLVEQGIGSISLNPDTVVATWKRLAHG
>Mature_778_residues
SRDYIVPLDALSMADVPRVGGKNASLGEMISRLADAGVKVPGGFATTAQAFWDFLDHNGLRARIGAVLDALDVTDVTALA
AAGAEIRAAVEGGRLPPALESGLREAYAALGDDVSVAVRSSATAEDLPDASFAGQQETYLHVRGAEALLAHLKKVFASLY
NDRAIAYRVHHGFAHADVALSAGVQRMVRADTGCSGVLFTLDTESGFRDVVFITAAYGLGETVVQGAVNPDEFYVHKPTL
ALGFPALVRRERGEKAIRMVWRDGKTVIEDTPPELRQRFALSDAEVLELARQAVQIERHYGRPMDVEWAKDGVSGELFIV
QARPETVKARAAAGGERYVLGGTGEVRVSGRAIGQKIGAGPVRVIATPAEMNRVQRGDVLVTDMTDPDWEPVMKRAAAIV
TNRGGRTCHAAIVARELGIPAVVGAGDATTVLRDDEAVTVSCAEGDTGFVYAGRLPFEVETLASESLPTPPVKLMMNVGN
PERAFEFAQIPNHGVGLARLEFIIARMIGVHPLALLDHAGQPQGVRDEIAARAAGYADPVAFYVDKLAEGIATLAAAFWP
QPVIVRLSDFKSNEYANLVGGERYEPKEENPMLGLRGAARYVSTLFRPAFELECRALKKVRETLGLGNVEVMIPFVRTVE
ECAAVVALLAANGLRRGEHGLRLIMMCEIPSNVLLADDFLRHVDGFSIGSNDLTQLTLGIDRDSGLVAEGFDERNPAVRK
LLAEGIAACRRNGKYIGICGQGPSDHPDLAQWLVEQGIGSISLNPDTVVATWKRLAHG

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=790, Percent_Identity=65.4430379746835, Blast_Score=1035, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=373, Percent_Identity=27.3458445040214, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI48994992, Length=190, Percent_Identity=34.2105263157895, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1788726, Length=191, Percent_Identity=32.4607329842932, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1789193, Length=407, Percent_Identity=26.7813267813268, Blast_Score=90, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17564524, Length=322, Percent_Identity=26.7080745341615, Blast_Score=76, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 83508; Mature: 83377

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRDYIVPLDALSMADVPRVGGKNASLGEMISRLADAGVKVPGGFATTAQAFWDFLDHNG
CCCCEEECHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC
LRARIGAVLDALDVTDVTALAAAGAEIRAAVEGGRLPPALESGLREAYAALGDDVSVAVR
HHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEE
SSATAEDLPDASFAGQQETYLHVRGAEALLAHLKKVFASLYNDRAIAYRVHHGFAHADVA
CCCCHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHH
LSAGVQRMVRADTGCSGVLFTLDTESGFRDVVFITAAYGLGETVVQGAVNPDEFYVHKPT
HHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEEECCCCHHHHHCCCCCCCEEEECCC
LALGFPALVRRERGEKAIRMVWRDGKTVIEDTPPELRQRFALSDAEVLELARQAVQIERH
HHHCCHHHHHHHCCCHHHHHHHCCCCEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
YGRPMDVEWAKDGVSGELFIVQARPETVKARAAAGGERYVLGGTGEVRVSGRAIGQKIGA
CCCCCCCHHHCCCCCCCEEEEECCCCHHHHHHCCCCCEEEECCCCEEEECCHHHHHHCCC
GPVRVIATPAEMNRVQRGDVLVTDMTDPDWEPVMKRAAAIVTNRGGRTCHAAIVARELGI
CCEEEEECCHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
PAVVGAGDATTVLRDDEAVTVSCAEGDTGFVYAGRLPFEVETLASESLPTPPVKLMMNVG
CEEECCCCCEEEEECCCEEEEEECCCCCCEEEECCCCCCHHHHHHCCCCCCCHHEEEECC
NPERAFEFAQIPNHGVGLARLEFIIARMIGVHPLALLDHAGQPQGVRDEIAARAAGYADP
CHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHCCCCCH
VAFYVDKLAEGIATLAAAFWPQPVIVRLSDFKSNEYANLVGGERYEPKEENPMLGLRGAA
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHCCCCCCCCCCCCCCCEECHHHH
RYVSTLFRPAFELECRALKKVRETLGLGNVEVMIPFVRTVEECAAVVALLAANGLRRGEH
HHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCC
GLRLIMMCEIPSNVLLADDFLRHVDGFSIGSNDLTQLTLGIDRDSGLVAEGFDERNPAVR
CCEEEEEEECCCCEEHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCEECCCCCCCHHHH
KLLAEGIAACRRNGKYIGICGQGPSDHPDLAQWLVEQGIGSISLNPDTVVATWKRLAHG
HHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SRDYIVPLDALSMADVPRVGGKNASLGEMISRLADAGVKVPGGFATTAQAFWDFLDHNG
CCCEEECHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC
LRARIGAVLDALDVTDVTALAAAGAEIRAAVEGGRLPPALESGLREAYAALGDDVSVAVR
HHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEE
SSATAEDLPDASFAGQQETYLHVRGAEALLAHLKKVFASLYNDRAIAYRVHHGFAHADVA
CCCCHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHH
LSAGVQRMVRADTGCSGVLFTLDTESGFRDVVFITAAYGLGETVVQGAVNPDEFYVHKPT
HHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEEECCCCHHHHHCCCCCCCEEEECCC
LALGFPALVRRERGEKAIRMVWRDGKTVIEDTPPELRQRFALSDAEVLELARQAVQIERH
HHHCCHHHHHHHCCCHHHHHHHCCCCEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
YGRPMDVEWAKDGVSGELFIVQARPETVKARAAAGGERYVLGGTGEVRVSGRAIGQKIGA
CCCCCCCHHHCCCCCCCEEEEECCCCHHHHHHCCCCCEEEECCCCEEEECCHHHHHHCCC
GPVRVIATPAEMNRVQRGDVLVTDMTDPDWEPVMKRAAAIVTNRGGRTCHAAIVARELGI
CCEEEEECCHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
PAVVGAGDATTVLRDDEAVTVSCAEGDTGFVYAGRLPFEVETLASESLPTPPVKLMMNVG
CEEECCCCCEEEEECCCEEEEEECCCCCCEEEECCCCCCHHHHHHCCCCCCCHHEEEECC
NPERAFEFAQIPNHGVGLARLEFIIARMIGVHPLALLDHAGQPQGVRDEIAARAAGYADP
CHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHCCCCCH
VAFYVDKLAEGIATLAAAFWPQPVIVRLSDFKSNEYANLVGGERYEPKEENPMLGLRGAA
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHCCCCCCCCCCCCCCCEECHHHH
RYVSTLFRPAFELECRALKKVRETLGLGNVEVMIPFVRTVEECAAVVALLAANGLRRGEH
HHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCC
GLRLIMMCEIPSNVLLADDFLRHVDGFSIGSNDLTQLTLGIDRDSGLVAEGFDERNPAVR
CCEEEEEEECCCCEEHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCEECCCCCCCHHHH
KLLAEGIAACRRNGKYIGICGQGPSDHPDLAQWLVEQGIGSISLNPDTVVATWKRLAHG
HHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1310524; 9097039; 9278503 [H]