Click here to switch to the map view.

The map label for this gene is pyrG

Identifier: 74316637

GI number: 74316637

Start: 650622

End: 652262

Strand: Direct

Name: pyrG

Synonym: Tbd_0619

Alternate gene names: 74316637

Gene position: 650622-652262 (Clockwise)

Preceding gene: 74316636

Following gene: 74316638

Centisome position: 22.36

GC content: 63.86

Gene sequence:

>1641_bases
ATGACGAAGTATGTGTTTGTCACCGGTGGGGTGGTTTCTTCCCTCGGGAAAGGGATCGCCTCCGCTTCACTCGCTGCGCT
GCTCGAATCGCGCGGCCTCCGTGTCACCCTGTTAAAGCTCGATCCCTACATCAACGTGGATCCGGGCACGATGAGCCCGT
TCCAGCACGGCGAAGTGTTCGTGACCGACGACGGCGCCGAGACCGACCTCGACCTCGGTCACTACGAGCGCTTCTCGAGC
GCGCGCATGAGCAAGCGCAACAACTTCACGACCGGACAGATCTACAAGTCGGTGATCGACAAGGAGCGCCGCGGCGACTA
CCTCGGCGGCACCGTGCAGGTCATTCCGCACATCACCGACGAGATCAAGCTCTCGATCCGCGAGGGCGCGAAGGGTGCCG
ACGTCGCGCTGGTCGAGATCGGCGGCACGGTCGGCGACATCGAGTCGCTGCCGTTTCTCGAAGCGATCCGCCAGATGGGT
TTCGAGGACGGCCCGGCGAATACCTGCTTCATCCATCTGACGCTCTTGCCCTATATCCCGACTGCCGGCGAGCTGAAGAC
CAAGCCGACCCAGCACTCGGTCAAGGAGTTGCGCGAGATCGGCATCCAGCCCGACATCCTGCTGTGCCGCGCCGACCGCG
AGATTCCGCTCGACGAGCGGCGCAAGATCGCGCTCTTCACCAACGTGCGCCCCGAGGCCGTGATCGAGGTGCTCGACGCC
GATTCGATCTACAAGATTCCGGCCATGCTGCACGAGCAGATGCTCGACGAGATCGTCTGCCACAAGCTCAACATCCTCGC
GCGCGCCGCCGACCTTTCGGTATGGAAGAGGCTCGTCGACGCACTCGAGCATCCCGAGCATACGGTCAACATCGCCTTCG
TCGGCAAGTACGTCGACCTGACCGAATCCTACAAGTCGCTGTCCGAAGCGATGATCCATGCCGGCATGCACACCAAAAGC
CGCGTCAAGATCCATTACGTCGACTCCGAGCAGATCGAGAAATCCGGCGTCGACTGTCTGCGCGGCATGGACGCGATCCT
CGTGCCCGGCGGCTTCGGCAAGCGCGGCGTCGAGGGCAAGATCGCGGCGATCCGCTACGCGCGCGAAAATCGCGTGCCGT
ATCTGGGCATCTGCCTCGGCATGCAGCTCGCCGTGGTCGAGTTCGCGCGCGACGTCGCCGGCATGGCCGACGCGCAGTCG
ACCGAATTCGATCCGACTACGAACCATCCCGTGATCGGTCTCATCACCGAATGGCTCGACCGCACGGGTCAGGTCGAAAA
GCGCAGCGAGCAATCCGACCTCGGCGGCACGATGCGCCTGGGCGGCCAGCCGTGCACGCTCAAGAACGGCACGCTGGCCC
GCGAGATCTATGGCGCCGACAGCATCGTCGAGCGGCATCGCCATCGCTACGAGGTCAACAACACGCTACTGAGCGAACTC
GAGGCCAAGGGTCTCGTCGTCGCGGGACGCGCGCCGGGTACCGAGCTGTGCGAAATGGTCGAGCTGCCGCCGGCCGTCCA
CCCCTGGTTCGTCGGCTGCCAGTTCCATCCGGAATTCACCAGCAACCCGCGCAACGGACACCCGCTGTTCATCTCGTTCG
TGAGAGCCGCGCTGGCGCATCAGCAAAAGGACTCCGCATGA

Upstream 100 bases:

>100_bases
ATGCGGGGGCGGCCATCGCGGGCGGTACGCTGCCCGCGCTGTCATTGAGCGCTCATCGGGGATGAAGTAAAATCCGCCAT
CACCCGCCCCCGCCCAGAAA

Downstream 100 bases:

>100_bases
AGCTGTGTCATTTCGAGGCGGGCCTCGACCAGCCGCTGTTCCTGATCTCGGGCCCTTGCGTGATCGAATCCGAGCAGCTC
GCGATGGACACCGCCGGCCA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MTKYVFVTGGVVSSLGKGIASASLAALLESRGLRVTLLKLDPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFSS
ARMSKRNNFTTGQIYKSVIDKERRGDYLGGTVQVIPHITDEIKLSIREGAKGADVALVEIGGTVGDIESLPFLEAIRQMG
FEDGPANTCFIHLTLLPYIPTAGELKTKPTQHSVKELREIGIQPDILLCRADREIPLDERRKIALFTNVRPEAVIEVLDA
DSIYKIPAMLHEQMLDEIVCHKLNILARAADLSVWKRLVDALEHPEHTVNIAFVGKYVDLTESYKSLSEAMIHAGMHTKS
RVKIHYVDSEQIEKSGVDCLRGMDAILVPGGFGKRGVEGKIAAIRYARENRVPYLGICLGMQLAVVEFARDVAGMADAQS
TEFDPTTNHPVIGLITEWLDRTGQVEKRSEQSDLGGTMRLGGQPCTLKNGTLAREIYGADSIVERHRHRYEVNNTLLSEL
EAKGLVVAGRAPGTELCEMVELPPAVHPWFVGCQFHPEFTSNPRNGHPLFISFVRAALAHQQKDSA

Sequences:

>Translated_546_residues
MTKYVFVTGGVVSSLGKGIASASLAALLESRGLRVTLLKLDPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFSS
ARMSKRNNFTTGQIYKSVIDKERRGDYLGGTVQVIPHITDEIKLSIREGAKGADVALVEIGGTVGDIESLPFLEAIRQMG
FEDGPANTCFIHLTLLPYIPTAGELKTKPTQHSVKELREIGIQPDILLCRADREIPLDERRKIALFTNVRPEAVIEVLDA
DSIYKIPAMLHEQMLDEIVCHKLNILARAADLSVWKRLVDALEHPEHTVNIAFVGKYVDLTESYKSLSEAMIHAGMHTKS
RVKIHYVDSEQIEKSGVDCLRGMDAILVPGGFGKRGVEGKIAAIRYARENRVPYLGICLGMQLAVVEFARDVAGMADAQS
TEFDPTTNHPVIGLITEWLDRTGQVEKRSEQSDLGGTMRLGGQPCTLKNGTLAREIYGADSIVERHRHRYEVNNTLLSEL
EAKGLVVAGRAPGTELCEMVELPPAVHPWFVGCQFHPEFTSNPRNGHPLFISFVRAALAHQQKDSA
>Mature_545_residues
TKYVFVTGGVVSSLGKGIASASLAALLESRGLRVTLLKLDPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFSSA
RMSKRNNFTTGQIYKSVIDKERRGDYLGGTVQVIPHITDEIKLSIREGAKGADVALVEIGGTVGDIESLPFLEAIRQMGF
EDGPANTCFIHLTLLPYIPTAGELKTKPTQHSVKELREIGIQPDILLCRADREIPLDERRKIALFTNVRPEAVIEVLDAD
SIYKIPAMLHEQMLDEIVCHKLNILARAADLSVWKRLVDALEHPEHTVNIAFVGKYVDLTESYKSLSEAMIHAGMHTKSR
VKIHYVDSEQIEKSGVDCLRGMDAILVPGGFGKRGVEGKIAAIRYARENRVPYLGICLGMQLAVVEFARDVAGMADAQST
EFDPTTNHPVIGLITEWLDRTGQVEKRSEQSDLGGTMRLGGQPCTLKNGTLAREIYGADSIVERHRHRYEVNNTLLSELE
AKGLVVAGRAPGTELCEMVELPPAVHPWFVGCQFHPEFTSNPRNGHPLFISFVRAALAHQQKDSA

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=561, Percent_Identity=46.1675579322638, Blast_Score=483, Evalue=1e-136,
Organism=Homo sapiens, GI28559085, Length=559, Percent_Identity=43.4704830053667, Blast_Score=467, Evalue=1e-131,
Organism=Homo sapiens, GI28559083, Length=559, Percent_Identity=43.4704830053667, Blast_Score=467, Evalue=1e-131,
Organism=Homo sapiens, GI221316689, Length=559, Percent_Identity=43.4704830053667, Blast_Score=467, Evalue=1e-131,
Organism=Escherichia coli, GI1789142, Length=544, Percent_Identity=63.0514705882353, Blast_Score=703, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25148299, Length=609, Percent_Identity=38.423645320197, Blast_Score=424, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6322563, Length=575, Percent_Identity=41.5652173913044, Blast_Score=446, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6319432, Length=571, Percent_Identity=42.031523642732, Blast_Score=442, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24664469, Length=560, Percent_Identity=43.3928571428571, Blast_Score=454, Evalue=1e-128,
Organism=Drosophila melanogaster, GI21357815, Length=505, Percent_Identity=41.7821782178218, Blast_Score=383, Evalue=1e-106,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_THIDA (Q3SL45)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314377.1
- HSSP:   P0A7E5
- ProteinModelPortal:   Q3SL45
- SMR:   Q3SL45
- STRING:   Q3SL45
- GeneID:   3673730
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0619
- NMPDR:   fig|292415.3.peg.506
- eggNOG:   COG0504
- HOGENOM:   HBG597806
- OMA:   RVTMQKL
- PhylomeDB:   Q3SL45
- ProtClustDB:   PRK05380
- BioCyc:   TDEN292415:TBD_0619-MONOMER
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 60185; Mature: 60054

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 378-378 ACT_SITE 516-516 ACT_SITE 518-518

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYVFVTGGVVSSLGKGIASASLAALLESRGLRVTLLKLDPYINVDPGTMSPFQHGEVF
CCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFSSARMSKRNNFTTGQIYKSVIDKERRGDYLGGTVQVIPHITD
EECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
EIKLSIREGAKGADVALVEIGGTVGDIESLPFLEAIRQMGFEDGPANTCFIHLTLLPYIP
HHEEEECCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEEEECCCC
TAGELKTKPTQHSVKELREIGIQPDILLCRADREIPLDERRKIALFTNVRPEAVIEVLDA
CCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHCC
DSIYKIPAMLHEQMLDEIVCHKLNILARAADLSVWKRLVDALEHPEHTVNIAFVGKYVDL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEHHHH
TESYKSLSEAMIHAGMHTKSRVKIHYVDSEQIEKSGVDCLRGMDAILVPGGFGKRGVEGK
HHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
IAAIRYARENRVPYLGICLGMQLAVVEFARDVAGMADAQSTEFDPTTNHPVIGLITEWLD
EEEEEEHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
RTGQVEKRSEQSDLGGTMRLGGQPCTLKNGTLAREIYGADSIVERHRHRYEVNNTLLSEL
CCCCHHHHCCHHCCCCEEEECCCCCEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
EAKGLVVAGRAPGTELCEMVELPPAVHPWFVGCQFHPEFTSNPRNGHPLFISFVRAALAH
HHCCEEEECCCCCHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
QQKDSA
HHCCCC
>Mature Secondary Structure 
TKYVFVTGGVVSSLGKGIASASLAALLESRGLRVTLLKLDPYINVDPGTMSPFQHGEVF
CEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFSSARMSKRNNFTTGQIYKSVIDKERRGDYLGGTVQVIPHITD
EECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
EIKLSIREGAKGADVALVEIGGTVGDIESLPFLEAIRQMGFEDGPANTCFIHLTLLPYIP
HHEEEECCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEEEECCCC
TAGELKTKPTQHSVKELREIGIQPDILLCRADREIPLDERRKIALFTNVRPEAVIEVLDA
CCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHCC
DSIYKIPAMLHEQMLDEIVCHKLNILARAADLSVWKRLVDALEHPEHTVNIAFVGKYVDL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEHHHH
TESYKSLSEAMIHAGMHTKSRVKIHYVDSEQIEKSGVDCLRGMDAILVPGGFGKRGVEGK
HHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
IAAIRYARENRVPYLGICLGMQLAVVEFARDVAGMADAQSTEFDPTTNHPVIGLITEWLD
EEEEEEHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
RTGQVEKRSEQSDLGGTMRLGGQPCTLKNGTLAREIYGADSIVERHRHRYEVNNTLLSEL
CCCCHHHHCCHHCCCCEEEECCCCCEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
EAKGLVVAGRAPGTELCEMVELPPAVHPWFVGCQFHPEFTSNPRNGHPLFISFVRAALAH
HHCCEEEECCCCCHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
QQKDSA
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA