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The map label for this gene is slyD [H]

Identifier: 74316605

GI number: 74316605

Start: 616316

End: 616798

Strand: Direct

Name: slyD [H]

Synonym: Tbd_0587

Alternate gene names: 74316605

Gene position: 616316-616798 (Clockwise)

Preceding gene: 74316604

Following gene: 74316606

Centisome position: 21.18

GC content: 64.8

Gene sequence:

>483_bases
GTGAACATCGGCAAAGACACCGTGGTGACGATCACCTACGTCGTGAAGGATCTGGACGGCAAGCTGCTCGAGGAAAGCGA
CGAGCCGGTCAGCTATCTGCACGGCGGCTACGACAACATCTTTCCGCTCGTCGAGCAGGCGCTCGAGGGCAAGGCGGTGG
GCGATGCGGTCGATCTCAAGTTGCAGCCGGCCGATGCCTTCGGTGAGTTCGAGGAGGACCTGGTCCGCCTCGAGCCGCGC
GAGGCGTTTCCACCCGAGATCGAGATCGGCATGCAGTTCGTCGGCTCGCCGGTCGACGGGGGCGGCGAGATGCTTTACAC
CGTGACCGACATCGCCGAAGAAAAAGTCGTCGTCGACGGCAACCATCCCTACGCGGGGCAGGCCGTGCATTTCCACTGCG
AGGTCAGCGAGGTGCGGGCCGCGACGTCCGACGAAGTCGAGCACGGTCACGCCCACGGCGCGCACGGCCACCATCACCAC
TGA

Upstream 100 bases:

>100_bases
CTTTCATCATCGACAAACAGGGCGTCATCCGTCACGCCCTGTACGGCGTTTCGAGCCGCGGCCACGCGGCGGAAGTGCTT
CAACTGGTAAAGGAACTCAA

Downstream 100 bases:

>100_bases
GCCAGGACGTCCCCCACACGCACGGGCGGGTCGCCGGGCTGCTAGAATGCCCGGCTAACCTCTTTGCCCTGACTGTCTGA
TGAAAACCACTTTCCTCGAT

Product: fkbp-type peptidyl-prolyl cis-trans isomerase

Products: NA

Alternate protein names: PPIase; Metallochaperone slyD [H]

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MNIGKDTVVTITYVVKDLDGKLLEESDEPVSYLHGGYDNIFPLVEQALEGKAVGDAVDLKLQPADAFGEFEEDLVRLEPR
EAFPPEIEIGMQFVGSPVDGGGEMLYTVTDIAEEKVVVDGNHPYAGQAVHFHCEVSEVRAATSDEVEHGHAHGAHGHHHH

Sequences:

>Translated_160_residues
MNIGKDTVVTITYVVKDLDGKLLEESDEPVSYLHGGYDNIFPLVEQALEGKAVGDAVDLKLQPADAFGEFEEDLVRLEPR
EAFPPEIEIGMQFVGSPVDGGGEMLYTVTDIAEEKVVVDGNHPYAGQAVHFHCEVSEVRAATSDEVEHGHAHGAHGHHHH
>Mature_160_residues
MNIGKDTVVTITYVVKDLDGKLLEESDEPVSYLHGGYDNIFPLVEQALEGKAVGDAVDLKLQPADAFGEFEEDLVRLEPR
EAFPPEIEIGMQFVGSPVDGGGEMLYTVTDIAEEKVVVDGNHPYAGQAVHFHCEVSEVRAATSDEVEHGHAHGAHGHHHH

Specific function: Also involved in hydrogenase metallocenter assembly, probably by participating in the nickel insertion step. This function in hydrogenase biosynthesis requires chaperone activity and the presence of the metal-binding domain, but not PPIase activity [H]

COG id: COG1047

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerases 2

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1789748, Length=150, Percent_Identity=38, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1786211, Length=150, Percent_Identity=32.6666666666667, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179 [H]

Pfam domain/function: PF00254 FKBP_C [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 17421; Mature: 17421

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIGKDTVVTITYVVKDLDGKLLEESDEPVSYLHGGYDNIFPLVEQALEGKAVGDAVDLK
CCCCCCCEEEEEEEEECCCCHHHCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEE
LQPADAFGEFEEDLVRLEPREAFPPEIEIGMQFVGSPVDGGGEMLYTVTDIAEEKVVVDG
ECCCHHHHHHHHHHHCCCCCCCCCCCCEECHHEECCCCCCCCCEEEEEECCCCCEEEEEC
NHPYAGQAVHFHCEVSEVRAATSDEVEHGHAHGAHGHHHH
CCCCCCCEEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNIGKDTVVTITYVVKDLDGKLLEESDEPVSYLHGGYDNIFPLVEQALEGKAVGDAVDLK
CCCCCCCEEEEEEEEECCCCHHHCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEE
LQPADAFGEFEEDLVRLEPREAFPPEIEIGMQFVGSPVDGGGEMLYTVTDIAEEKVVVDG
ECCCHHHHHHHHHHHCCCCCCCCCCCCEECHHEECCCCCCCCCEEEEEECCCCCEEEEEC
NHPYAGQAVHFHCEVSEVRAATSDEVEHGHAHGAHGHHHH
CCCCCCCEEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]