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The map label for this gene is kefB [H]

Identifier: 74316557

GI number: 74316557

Start: 562894

End: 564870

Strand: Direct

Name: kefB [H]

Synonym: Tbd_0539

Alternate gene names: 74316557

Gene position: 562894-564870 (Clockwise)

Preceding gene: 74316540

Following gene: 74316559

Centisome position: 19.34

GC content: 67.37

Gene sequence:

>1977_bases
ATGCACTCCGGTAGCAGCCTTCAGCTCGTCCTGATCCTGCTCGCGGTGGCGGTGCTGGTGGCGGCCGCGGCGCGTGCGCT
GCGCCTGCCGACCATGCTCGCCTATCTCGTCACCGGGATCGCGATCGGCCCGTTCGCGCTCGGCTGGATTCCGGACAGCG
ACGAGGCGCGTTATCTGGCCGAATTCGGCGTCGTGTTCCTGATGTTTTCGATCGGCCTCGAATTCAGCCTGCCGCGTCTG
ATGACAATGCGCATGACCGTGTTCGGCTTCGGCGGCGCACAGGTCGTGCTGACCATTGTCCTCAGCGCCGCGGTCGCCTG
GATGCTCGGATTGCCCCCGCTCGCCGCGCTCGCCGTCGGCGGCATCATGTCGATGTCGTCGACCGCGATCGTCTCGAAAA
TGCTGACCGAACGGCTCGAGGTGCAGACCTCGCACGGACGCCAGATCTTCGGGGCGCTGCTGTTTCAGGATCTCGCAGTG
GTGCCGCTGCTGATCCTGATCCCGGCTTTTGCCAAGGAATCGGACGCGATGGCCGCGACCCTCGGTCTGGCCTTGCTCAA
GGCGACGGTCGTCCTCGGCTTCCTACTGTTCGTCGGTCAGCGCGTCATGCGGCCGTGGTTCCAGTGGGTCGCGGGGCACA
AGTCGCCCGAGCTTTTCACCCTCAATTTGCTGCTTTTCACGCTCGGCCTCGCCTTCCTGACCGAGAGCGCCGGCCTGTCG
CTGGCGCTCGGGGCCTTCCTCGCTGGCATGCTGGTGTCCGAGACCGAATACCGCTACCAAGTCGAGGACGACATCAAGCC
TTTCCGGGACGTGCTCCTGGGCCTGTTCTTCGTCACGATCGGCATGCGTCTCGACCCCACGCAGGTGATGCTCGACTGGG
CCGACGTGCTCATGATCGTCGCGGCGATCGTCATCGGCAAGGGCGTGCTCGTCGCGGGTCTCGCAATGGCCTTCGGCAGT
GGCCGGCCGACCGCGTGGCGCACCGCTCTTGGGCTCGCGCAGGCGGGCGAATTCGGCTTCGTTCTGCTCGCGCAGGCGGC
CGACCTCAGGCTGCTCGGCGTCGAGGTCGCCCAGCCGCTGCTCGCCGCGATGGTGCTGTCGATGCTGATCGCTCCGCTGC
TGATCCACCGGATGGACACCCTCACGCGGCTTCTTGCCGGCAGCGAGTGGTCCGGGCGCGCCAAGGAAGTGCACGACATT
TCGATCAAGAGCTTCGGCAAGACCCGCCACGTGATCGTCTGCGGCTACGGGCGCAGCGGCCAGAATCTGGCGCGCCTGCT
CGAGGCCGAAGGCATCACCTTTATCGCACTCGACGCCGACCCCGAGCGCATCCGCGCGGTCGCAGCGTCAGGCGTTTCGG
CCGTCTACGGCGATGCGAGCCGGCGTGAAGTGCTGGTCGCCGCGGGCTTGAGCCGGGCGCAGGCCGTCGTTGTCACCTAT
TCCGACGTGCATTCGAGCATGGCCATCCTGCGTCATGTGCGCGAGCTGCGACCGGAGGTCCCGGTCATCGTGCGCACGAT
CGACGACACCCATATCGACGCCTTGAAGGCGGCCGGCGCGGCCGAAGTCGTGTCGGAAGTGATGGAAGGCTCGCTGATGC
TCGCCTCGCACGCGCTGATGCTGCTCGGCGTGCCGCTCGCCCAGGTGCTGAGGCGGGTGCGTGACGTCCGCGACACGCGT
TACGCGCTGATGCGCGGGTTTTTCCGTGGCGCGAGCGACGTCGACCACGAACTCGACCAGCAGAGCCAGCCGCGCCTGAA
CACCGTGCTGATCACCCAGGGCTCGGCCGCGGCGGGGCACACGCTCGCGGAACTGGCCTTGGACGAATTGGCGGTCGAGG
TGGTCGCGATCCGCCGTCAGGGCATCAAGGGCGTCGACCCGCAGCCCGAGGCCGAAATCCGCGTGGGCGACGTGCTGGTG
TTACGGGGCGGCGCCGGGGGGCTGGCGGCGGCGGAGTTCAGGTTATTGCAGGGCTGA

Upstream 100 bases:

>100_bases
AGCGTATCGCGCCCCCCCGAATAACACTCAGCCATCGACGACCGCATGCCCTTCACGAGGACGCCGACTTCAGCACCCAC
CCGCGCCACCGACACTTCGC

Downstream 100 bases:

>100_bases
AGCGTTCTCCGAGCGCTAGTCCGGTCGGGACGCCCTCAGCGACGGACCGAAAAGAAACGCGGGACTTATACGCCCATGCA
AACGGTGTACGTGGTGGAAT

Product: antiporter-2, CPA2 family monovalent cation/proton antiporter

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 658; Mature: 658

Protein sequence:

>658_residues
MHSGSSLQLVLILLAVAVLVAAAARALRLPTMLAYLVTGIAIGPFALGWIPDSDEARYLAEFGVVFLMFSIGLEFSLPRL
MTMRMTVFGFGGAQVVLTIVLSAAVAWMLGLPPLAALAVGGIMSMSSTAIVSKMLTERLEVQTSHGRQIFGALLFQDLAV
VPLLILIPAFAKESDAMAATLGLALLKATVVLGFLLFVGQRVMRPWFQWVAGHKSPELFTLNLLLFTLGLAFLTESAGLS
LALGAFLAGMLVSETEYRYQVEDDIKPFRDVLLGLFFVTIGMRLDPTQVMLDWADVLMIVAAIVIGKGVLVAGLAMAFGS
GRPTAWRTALGLAQAGEFGFVLLAQAADLRLLGVEVAQPLLAAMVLSMLIAPLLIHRMDTLTRLLAGSEWSGRAKEVHDI
SIKSFGKTRHVIVCGYGRSGQNLARLLEAEGITFIALDADPERIRAVAASGVSAVYGDASRREVLVAAGLSRAQAVVVTY
SDVHSSMAILRHVRELRPEVPVIVRTIDDTHIDALKAAGAAEVVSEVMEGSLMLASHALMLLGVPLAQVLRRVRDVRDTR
YALMRGFFRGASDVDHELDQQSQPRLNTVLITQGSAAAGHTLAELALDELAVEVVAIRRQGIKGVDPQPEAEIRVGDVLV
LRGGAGGLAAAEFRLLQG

Sequences:

>Translated_658_residues
MHSGSSLQLVLILLAVAVLVAAAARALRLPTMLAYLVTGIAIGPFALGWIPDSDEARYLAEFGVVFLMFSIGLEFSLPRL
MTMRMTVFGFGGAQVVLTIVLSAAVAWMLGLPPLAALAVGGIMSMSSTAIVSKMLTERLEVQTSHGRQIFGALLFQDLAV
VPLLILIPAFAKESDAMAATLGLALLKATVVLGFLLFVGQRVMRPWFQWVAGHKSPELFTLNLLLFTLGLAFLTESAGLS
LALGAFLAGMLVSETEYRYQVEDDIKPFRDVLLGLFFVTIGMRLDPTQVMLDWADVLMIVAAIVIGKGVLVAGLAMAFGS
GRPTAWRTALGLAQAGEFGFVLLAQAADLRLLGVEVAQPLLAAMVLSMLIAPLLIHRMDTLTRLLAGSEWSGRAKEVHDI
SIKSFGKTRHVIVCGYGRSGQNLARLLEAEGITFIALDADPERIRAVAASGVSAVYGDASRREVLVAAGLSRAQAVVVTY
SDVHSSMAILRHVRELRPEVPVIVRTIDDTHIDALKAAGAAEVVSEVMEGSLMLASHALMLLGVPLAQVLRRVRDVRDTR
YALMRGFFRGASDVDHELDQQSQPRLNTVLITQGSAAAGHTLAELALDELAVEVVAIRRQGIKGVDPQPEAEIRVGDVLV
LRGGAGGLAAAEFRLLQG
>Mature_658_residues
MHSGSSLQLVLILLAVAVLVAAAARALRLPTMLAYLVTGIAIGPFALGWIPDSDEARYLAEFGVVFLMFSIGLEFSLPRL
MTMRMTVFGFGGAQVVLTIVLSAAVAWMLGLPPLAALAVGGIMSMSSTAIVSKMLTERLEVQTSHGRQIFGALLFQDLAV
VPLLILIPAFAKESDAMAATLGLALLKATVVLGFLLFVGQRVMRPWFQWVAGHKSPELFTLNLLLFTLGLAFLTESAGLS
LALGAFLAGMLVSETEYRYQVEDDIKPFRDVLLGLFFVTIGMRLDPTQVMLDWADVLMIVAAIVIGKGVLVAGLAMAFGS
GRPTAWRTALGLAQAGEFGFVLLAQAADLRLLGVEVAQPLLAAMVLSMLIAPLLIHRMDTLTRLLAGSEWSGRAKEVHDI
SIKSFGKTRHVIVCGYGRSGQNLARLLEAEGITFIALDADPERIRAVAASGVSAVYGDASRREVLVAAGLSRAQAVVVTY
SDVHSSMAILRHVRELRPEVPVIVRTIDDTHIDALKAAGAAEVVSEVMEGSLMLASHALMLLGVPLAQVLRRVRDVRDTR
YALMRGFFRGASDVDHELDQQSQPRLNTVLITQGSAAAGHTLAELALDELAVEVVAIRRQGIKGVDPQPEAEIRVGDVLV
LRGGAGGLAAAEFRLLQG

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=376, Percent_Identity=27.3936170212766, Blast_Score=113, Evalue=7e-25,
Organism=Escherichia coli, GI1786232, Length=554, Percent_Identity=34.6570397111913, Blast_Score=269, Evalue=3e-73,
Organism=Escherichia coli, GI1789749, Length=544, Percent_Identity=34.9264705882353, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI1786685, Length=534, Percent_Identity=30.8988764044944, Blast_Score=211, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 70304; Mature: 70304

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSGSSLQLVLILLAVAVLVAAAARALRLPTMLAYLVTGIAIGPFALGWIPDSDEARYLA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHH
EFGVVFLMFSIGLEFSLPRLMTMRMTVFGFGGAQVVLTIVLSAAVAWMLGLPPLAALAVG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
GIMSMSSTAIVSKMLTERLEVQTSHGRQIFGALLFQDLAVVPLLILIPAFAKESDAMAAT
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LGLALLKATVVLGFLLFVGQRVMRPWFQWVAGHKSPELFTLNLLLFTLGLAFLTESAGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHCCCHH
LALGAFLAGMLVSETEYRYQVEDDIKPFRDVLLGLFFVTIGMRLDPTQVMLDWADVLMIV
HHHHHHHHHHHHHCCHHEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AAIVIGKGVLVAGLAMAFGSGRPTAWRTALGLAQAGEFGFVLLAQAADLRLLGVEVAQPL
HHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCEEHHHHHHHH
LAAMVLSMLIAPLLIHRMDTLTRLLAGSEWSGRAKEVHDISIKSFGKTRHVIVCGYGRSG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHCCCCEEEEEEECCCCC
QNLARLLEAEGITFIALDADPERIRAVAASGVSAVYGDASRREVLVAAGLSRAQAVVVTY
HHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCHHHHCCCCHHHEEEEECCCCCEEEEEEH
SDVHSSMAILRHVRELRPEVPVIVRTIDDTHIDALKAAGAAEVVSEVMEGSLMLASHALM
HHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHH
LLGVPLAQVLRRVRDVRDTRYALMRGFFRGASDVDHELDQQSQPRLNTVLITQGSAAAGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCEEEEECCCCCCCH
TLAELALDELAVEVVAIRRQGIKGVDPQPEAEIRVGDVLVLRGGAGGLAAAEFRLLQG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCEEEEECCCCCHHHHHHHHCCC
>Mature Secondary Structure
MHSGSSLQLVLILLAVAVLVAAAARALRLPTMLAYLVTGIAIGPFALGWIPDSDEARYLA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHH
EFGVVFLMFSIGLEFSLPRLMTMRMTVFGFGGAQVVLTIVLSAAVAWMLGLPPLAALAVG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
GIMSMSSTAIVSKMLTERLEVQTSHGRQIFGALLFQDLAVVPLLILIPAFAKESDAMAAT
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LGLALLKATVVLGFLLFVGQRVMRPWFQWVAGHKSPELFTLNLLLFTLGLAFLTESAGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHCCCHH
LALGAFLAGMLVSETEYRYQVEDDIKPFRDVLLGLFFVTIGMRLDPTQVMLDWADVLMIV
HHHHHHHHHHHHHCCHHEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AAIVIGKGVLVAGLAMAFGSGRPTAWRTALGLAQAGEFGFVLLAQAADLRLLGVEVAQPL
HHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCEEHHHHHHHH
LAAMVLSMLIAPLLIHRMDTLTRLLAGSEWSGRAKEVHDISIKSFGKTRHVIVCGYGRSG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHCCCCEEEEEEECCCCC
QNLARLLEAEGITFIALDADPERIRAVAASGVSAVYGDASRREVLVAAGLSRAQAVVVTY
HHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCHHHHCCCCHHHEEEEECCCCCEEEEEEH
SDVHSSMAILRHVRELRPEVPVIVRTIDDTHIDALKAAGAAEVVSEVMEGSLMLASHALM
HHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHH
LLGVPLAQVLRRVRDVRDTRYALMRGFFRGASDVDHELDQQSQPRLNTVLITQGSAAAGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCEEEEECCCCCCCH
TLAELALDELAVEVVAIRRQGIKGVDPQPEAEIRVGDVLVLRGGAGGLAAAEFRLLQG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCEEEEECCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA