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The map label for this gene is bioH [H]

Identifier: 74316339

GI number: 74316339

Start: 345067

End: 345810

Strand: Direct

Name: bioH [H]

Synonym: Tbd_0321

Alternate gene names: 74316339

Gene position: 345067-345810 (Clockwise)

Preceding gene: 74316338

Following gene: 74316340

Centisome position: 11.86

GC content: 72.18

Gene sequence:

>744_bases
ATGAGCTCGCCTAAGCCGGTTCTCGTGCTGGTGCATGGCTGGGGCATGAACGCCCGCGTCTTCGATGCGCTCGCCGACCG
CCTCGATGACCGCTTCGACGTGCGCGGGTTCGACCTGCCGGGCCATGGCGGACGCGACGCGCAAGCGGTCAACACGCTCG
AGGCCTGGGCCGACGCGCTTGCCGATGCACTGCCCGACGACGCCAACCTGCTCGGCTGGTCGCTCGGCGGCCAGGTCGCG
TTGCGCGCCGCGCTCGATCACCCGAGGAAGGTCGCGCGCCTGATCCTCGTCGCAACGACGCCGCGGTTCGTCCAGCGCGC
GGACTGGGCGAGCGCGATGGACGCCGCCGATCTCGCCGCCTTCGGGGAGGCGCTCCTCGCGGACCCGGCCACAACGCTGC
AGCGGTTCCTCAGTCTGCAGACCCGCGGCACGCCGGGGCAGAAGGCGCTCCTGCAGCATTTGCGGCAGGCGCTCCGCGAG
GCGCCCGAGCCCGACCGGGTCGCACTGGCGGCGGGGCTCGCGATCCTCCGCGATACCGACTTGCGCGGCGACGTGTTGCG
GCTGACCCATCCGACGCTGGTGCTGCACGGCGCGCTCGACACGCTGACGCCCGCCGCCGCGGGGGCCTGGCTTGCGGCTA
ATCTGCGCGCAGCGCGCCACGTCGAACTCGAGCGGGCTGCGCACGCGCCGCATCTGTCGCATCCCGACGATGTCGCGCGC
GAGATCGAACGGTTTTGCCTATGA

Upstream 100 bases:

>100_bases
ATCCGCACGCCGACCGTGCCGGCGAATACCGCGCGGCTGCGCATCACGCTGTCCGCCGCGCACAGCGACGCCGACGTCGC
CCGACTCGTCGAGACCGTTC

Downstream 100 bases:

>100_bases
CCGAAGCCGAGCTTGATTACGCCGAGGTCCGCCGCGCCTTCGACCACGCGGCCGCCGCCTACGACGCGCATGCCGTGCTG
CAGCGCGAAGTCTGCGACCG

Product: BioH

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSSPKPVLVLVHGWGMNARVFDALADRLDDRFDVRGFDLPGHGGRDAQAVNTLEAWADALADALPDDANLLGWSLGGQVA
LRAALDHPRKVARLILVATTPRFVQRADWASAMDAADLAAFGEALLADPATTLQRFLSLQTRGTPGQKALLQHLRQALRE
APEPDRVALAAGLAILRDTDLRGDVLRLTHPTLVLHGALDTLTPAAAGAWLAANLRAARHVELERAAHAPHLSHPDDVAR
EIERFCL

Sequences:

>Translated_247_residues
MSSPKPVLVLVHGWGMNARVFDALADRLDDRFDVRGFDLPGHGGRDAQAVNTLEAWADALADALPDDANLLGWSLGGQVA
LRAALDHPRKVARLILVATTPRFVQRADWASAMDAADLAAFGEALLADPATTLQRFLSLQTRGTPGQKALLQHLRQALRE
APEPDRVALAAGLAILRDTDLRGDVLRLTHPTLVLHGALDTLTPAAAGAWLAANLRAARHVELERAAHAPHLSHPDDVAR
EIERFCL
>Mature_246_residues
SSPKPVLVLVHGWGMNARVFDALADRLDDRFDVRGFDLPGHGGRDAQAVNTLEAWADALADALPDDANLLGWSLGGQVAL
RAALDHPRKVARLILVATTPRFVQRADWASAMDAADLAAFGEALLADPATTLQRFLSLQTRGTPGQKALLQHLRQALREA
PEPDRVALAAGLAILRDTDLRGDVLRLTHPTLVLHGALDTLTPAAAGAWLAANLRAARHVELERAAHAPHLSHPDDVARE
IERFCL

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

Organism=Escherichia coli, GI1789817, Length=232, Percent_Identity=37.0689655172414, Blast_Score=138, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 26617; Mature: 26485

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPKPVLVLVHGWGMNARVFDALADRLDDRFDVRGFDLPGHGGRDAQAVNTLEAWADAL
CCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
ADALPDDANLLGWSLGGQVALRAALDHPRKVARLILVATTPRFVQRADWASAMDAADLAA
HHHCCCCCCEEEECCCHHHHHHHHHCCHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHH
FGEALLADPATTLQRFLSLQTRGTPGQKALLQHLRQALREAPEPDRVALAAGLAILRDTD
HHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
LRGDVLRLTHPTLVLHGALDTLTPAAAGAWLAANLRAARHVELERAAHAPHLSHPDDVAR
CCCCEEEECCCHHEEECCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
EIERFCL
HHHHHCC
>Mature Secondary Structure 
SSPKPVLVLVHGWGMNARVFDALADRLDDRFDVRGFDLPGHGGRDAQAVNTLEAWADAL
CCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
ADALPDDANLLGWSLGGQVALRAALDHPRKVARLILVATTPRFVQRADWASAMDAADLAA
HHHCCCCCCEEEECCCHHHHHHHHHCCHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHH
FGEALLADPATTLQRFLSLQTRGTPGQKALLQHLRQALREAPEPDRVALAAGLAILRDTD
HHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
LRGDVLRLTHPTLVLHGALDTLTPAAAGAWLAANLRAARHVELERAAHAPHLSHPDDVAR
CCCCEEEECCCHHEEECCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
EIERFCL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]