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The map label for this gene is bioF

Identifier: 74316338

GI number: 74316338

Start: 343920

End: 345080

Strand: Direct

Name: bioF

Synonym: Tbd_0320

Alternate gene names: 74316338

Gene position: 343920-345080 (Clockwise)

Preceding gene: 74316337

Following gene: 74316339

Centisome position: 11.82

GC content: 71.06

Gene sequence:

>1161_bases
GTGACCGACGGCGCGCTGACCCGCCTGGAACACGGGCTGGCGGCGCTGAAGGCCGATCATCTCGCGCGGCGGCGACCGCT
GCTCGACGGTGCGCAGGGCGCGCACGTCACGATCGACGGCCGCCGCGTCGTCTCGTTCTGCAGCAACGACTACCTCGGAC
TCGCCAACGATCCGGCGCTCGTCGCCGCCGCCCACGCGGCGCTCGACGGGTGTGGGGTCGGCGCCGGCGCGGCGCATCTG
ATCAGCGGCCATCATCGCCTGCATCTCGAGTTCGAGACGGACTTCGCGCAGTGGGTCGGCAAGCCCGCGGCCCTGCTGTT
TTCGACCGGCTATCTCGCCAACCTCGCGGTGGTCACGGCGCTCATTTCCCGCCGCGGCGAAGTCTTCGCCGACAAACTCA
ACCACGCCTCGCTGGTCGACGCGGCCAAGCTCTCGGGCGCGCGTTTCAGCCGCTATCGACACGGCGACCTCGCGCAACTC
GAAAGCCGCCTCGCCGCGAGCACGGCACGCGACCGCATGATCGTGTCCGACCTCGTGTTCAGCATGGACGGCGACGTCGC
GCCCGTCGACGCCTTGCTCGATCTCGCCGAGCGCTACGATGCCTGGCTCTACCTCGACGACGCGCACGGCTTCGGCGTGT
TGAACGGCGGTCGTGGCGGGCTCAGCGAGCGGGCGCGGGCGAGTACGCGCGTGATCTATCTGGCGACGCTCGGCAAGGCC
GCGGGCGTCGCGGGCGCGAGCGTCGCGGCGGACGGCAGGGTCATCGAGTGGCTGATCCAGACGGCGCGCCCGTACATCTA
TACGACCGCAGCGCCGCCGCTGCTCGCCGCGGCGCTGCGCGAAAGCCTGCGCCAGATCGCCGCCGGAGCCGCGCGCCGAG
GCCGCCTGCAACGCCACGTCGCTCGGTTGCGCAGCGGACTCGGGGGACTGAAGAACGCGGTGCTCGCCGACTCCTTCACG
CCGATTCAGCCGCTCATCGTCGGCGCCAACGCCGACGCCCTCACGTTGTCGCAGGCCTTGTTGCAACGCGGAATCCTCGT
GCCGGCGATCCGCACGCCGACCGTGCCGGCGAATACCGCGCGGCTGCGCATCACGCTGTCCGCCGCGCACAGCGACGCCG
ACGTCGCCCGACTCGTCGAGACCGTTCATGAGCTCGCCTAA

Upstream 100 bases:

>100_bases
GCGCCAATTCGATCTTCTACGGCGAAAAGCTCCTGACCACGGGCAACCCCGAGTGGGAGCGCGATCAGCGTCTGTTCGAC
AGCCTGGGCGTGACGGCGTT

Downstream 100 bases:

>100_bases
GCCGGTTCTCGTGCTGGTGCATGGCTGGGGCATGAACGCCCGCGTCTTCGATGCGCTCGCCGACCGCCTCGATGACCGCT
TCGACGTGCGCGGGTTCGAC

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MTDGALTRLEHGLAALKADHLARRRPLLDGAQGAHVTIDGRRVVSFCSNDYLGLANDPALVAAAHAALDGCGVGAGAAHL
ISGHHRLHLEFETDFAQWVGKPAALLFSTGYLANLAVVTALISRRGEVFADKLNHASLVDAAKLSGARFSRYRHGDLAQL
ESRLAASTARDRMIVSDLVFSMDGDVAPVDALLDLAERYDAWLYLDDAHGFGVLNGGRGGLSERARASTRVIYLATLGKA
AGVAGASVAADGRVIEWLIQTARPYIYTTAAPPLLAAALRESLRQIAAGAARRGRLQRHVARLRSGLGGLKNAVLADSFT
PIQPLIVGANADALTLSQALLQRGILVPAIRTPTVPANTARLRITLSAAHSDADVARLVETVHELA

Sequences:

>Translated_386_residues
MTDGALTRLEHGLAALKADHLARRRPLLDGAQGAHVTIDGRRVVSFCSNDYLGLANDPALVAAAHAALDGCGVGAGAAHL
ISGHHRLHLEFETDFAQWVGKPAALLFSTGYLANLAVVTALISRRGEVFADKLNHASLVDAAKLSGARFSRYRHGDLAQL
ESRLAASTARDRMIVSDLVFSMDGDVAPVDALLDLAERYDAWLYLDDAHGFGVLNGGRGGLSERARASTRVIYLATLGKA
AGVAGASVAADGRVIEWLIQTARPYIYTTAAPPLLAAALRESLRQIAAGAARRGRLQRHVARLRSGLGGLKNAVLADSFT
PIQPLIVGANADALTLSQALLQRGILVPAIRTPTVPANTARLRITLSAAHSDADVARLVETVHELA
>Mature_385_residues
TDGALTRLEHGLAALKADHLARRRPLLDGAQGAHVTIDGRRVVSFCSNDYLGLANDPALVAAAHAALDGCGVGAGAAHLI
SGHHRLHLEFETDFAQWVGKPAALLFSTGYLANLAVVTALISRRGEVFADKLNHASLVDAAKLSGARFSRYRHGDLAQLE
SRLAASTARDRMIVSDLVFSMDGDVAPVDALLDLAERYDAWLYLDDAHGFGVLNGGRGGLSERARASTRVIYLATLGKAA
GVAGASVAADGRVIEWLIQTARPYIYTTAAPPLLAAALRESLRQIAAGAARRGRLQRHVARLRSGLGGLKNAVLADSFTP
IQPLIVGANADALTLSQALLQRGILVPAIRTPTVPANTARLRITLSAAHSDADVARLVETVHELA

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily

Homologues:

Organism=Homo sapiens, GI7657118, Length=382, Percent_Identity=31.9371727748691, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI284448556, Length=346, Percent_Identity=33.2369942196532, Blast_Score=194, Evalue=8e-50,
Organism=Homo sapiens, GI83977442, Length=356, Percent_Identity=33.9887640449438, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI83977440, Length=354, Percent_Identity=33.8983050847458, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI83977444, Length=354, Percent_Identity=33.8983050847458, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI4502025, Length=392, Percent_Identity=29.8469387755102, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI40316939, Length=392, Percent_Identity=29.8469387755102, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI4758668, Length=363, Percent_Identity=25.6198347107438, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI119220554, Length=365, Percent_Identity=24.9315068493151, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI5454084, Length=396, Percent_Identity=23.7373737373737, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1786993, Length=362, Percent_Identity=42.2651933701657, Blast_Score=252, Evalue=3e-68,
Organism=Escherichia coli, GI1790046, Length=379, Percent_Identity=35.3562005277045, Blast_Score=222, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI71994529, Length=377, Percent_Identity=33.9522546419098, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI32566772, Length=425, Percent_Identity=25.8823529411765, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17560914, Length=362, Percent_Identity=25.414364640884, Blast_Score=120, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17560912, Length=362, Percent_Identity=25.414364640884, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71982617, Length=324, Percent_Identity=21.6049382716049, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI71982625, Length=324, Percent_Identity=21.6049382716049, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320438, Length=382, Percent_Identity=32.1989528795811, Blast_Score=169, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6320267, Length=363, Percent_Identity=27.5482093663912, Blast_Score=136, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6323954, Length=359, Percent_Identity=22.5626740947075, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24662918, Length=377, Percent_Identity=31.5649867374005, Blast_Score=224, Evalue=7e-59,
Organism=Drosophila melanogaster, GI17137420, Length=340, Percent_Identity=29.7058823529412, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI17136286, Length=366, Percent_Identity=24.5901639344262, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24653280, Length=324, Percent_Identity=22.8395061728395, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24653276, Length=324, Percent_Identity=22.8395061728395, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24653278, Length=324, Percent_Identity=22.8395061728395, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOF_THIDA (Q3SLX9)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314078.1
- HSSP:   P12998
- ProteinModelPortal:   Q3SLX9
- STRING:   Q3SLX9
- GeneID:   3672106
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0320
- NMPDR:   fig|292415.3.peg.552
- eggNOG:   COG0156
- HOGENOM:   HBG649839
- OMA:   PELASIC
- PhylomeDB:   Q3SLX9
- BioCyc:   TDEN292415:TBD_0320-MONOMER
- HAMAP:   MF_01693
- InterPro:   IPR022834
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR00858

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.47

Molecular weight: Translated: 40712; Mature: 40581

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 23-23 BINDING 135-135 BINDING 181-181 BINDING 354-354

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDGALTRLEHGLAALKADHLARRRPLLDGAQGAHVTIDGRRVVSFCSNDYLGLANDPAL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHCCCCCCCCCCCHH
VAAAHAALDGCGVGAGAAHLISGHHRLHLEFETDFAQWVGKPAALLFSTGYLANLAVVTA
HHHHHHHHHCCCCCCCHHHHHCCCEEEEEEECCCHHHHCCCCHHHHHHCCHHHHHHHHHH
LISRRGEVFADKLNHASLVDAAKLSGARFSRYRHGDLAQLESRLAASTARDRMIVSDLVF
HHHHCCHHHHHHCCCHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SMDGDVAPVDALLDLAERYDAWLYLDDAHGFGVLNGGRGGLSERARASTRVIYLATLGKA
HCCCCCCHHHHHHHHHHHCCEEEEEECCCCCEEEECCCCCCCHHHHHHCEEEEEEECCCH
AGVAGASVAADGRVIEWLIQTARPYIYTTAAPPLLAAALRESLRQIAAGAARRGRLQRHV
HCCCCCCCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARLRSGLGGLKNAVLADSFTPIQPLIVGANADALTLSQALLQRGILVPAIRTPTVPANTA
HHHHHCCCHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHCCCEECCCCCCCCCCCCE
RLRITLSAAHSDADVARLVETVHELA
EEEEEEEECCCCHHHHHHHHHHHHHC
>Mature Secondary Structure 
TDGALTRLEHGLAALKADHLARRRPLLDGAQGAHVTIDGRRVVSFCSNDYLGLANDPAL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHCCCCCCCCCCCHH
VAAAHAALDGCGVGAGAAHLISGHHRLHLEFETDFAQWVGKPAALLFSTGYLANLAVVTA
HHHHHHHHHCCCCCCCHHHHHCCCEEEEEEECCCHHHHCCCCHHHHHHCCHHHHHHHHHH
LISRRGEVFADKLNHASLVDAAKLSGARFSRYRHGDLAQLESRLAASTARDRMIVSDLVF
HHHHCCHHHHHHCCCHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SMDGDVAPVDALLDLAERYDAWLYLDDAHGFGVLNGGRGGLSERARASTRVIYLATLGKA
HCCCCCCHHHHHHHHHHHCCEEEEEECCCCCEEEECCCCCCCHHHHHHCEEEEEEECCCH
AGVAGASVAADGRVIEWLIQTARPYIYTTAAPPLLAAALRESLRQIAAGAARRGRLQRHV
HCCCCCCCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARLRSGLGGLKNAVLADSFTPIQPLIVGANADALTLSQALLQRGILVPAIRTPTVPANTA
HHHHHCCCHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHCCCEECCCCCCCCCCCCE
RLRITLSAAHSDADVARLVETVHELA
EEEEEEEECCCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA