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The map label for this gene is ubiE

Identifier: 74316233

GI number: 74316233

Start: 235913

End: 236647

Strand: Reverse

Name: ubiE

Synonym: Tbd_0215

Alternate gene names: 74316233

Gene position: 236647-235913 (Counterclockwise)

Preceding gene: 74316236

Following gene: 74316231

Centisome position: 8.13

GC content: 64.49

Gene sequence:

>735_bases
ATGGAAAAAACCACCCACTTTGGCTACCAGGAGGTCGCCGAATCCGAGAAAGCCGCCAAGGTGGCCGAGGTCTTCCATTC
GGTCGCGGCGCAATACGACATCATGAACGACCTGATGTCGGCGGGCCTGCACCGCCTCTGGAAGCGCTTCGCCGTCGCGC
AGGCAGGGCTGCGGCCCGGCATGAAGGTGCTCGACGTGGCCGGCGGCACGGCCGACCTCACGCGCCTCTTCCTCAAGGAA
GTCGGCGCGAGCGGCACGGTTTTGCTGACCGACATCAACTTCTCGATGCTGCGCGAAGGCCGCGACCGCATGCTCAACGA
GGGCAGAACGCCGCCCGCGGTGCAGTGCGACGGCGAACGCCTGCCCTTTCCCGACGATTACTTCGATTGCGTGTCGGTCG
CGTTCGGCCTGCGCAACATGACGCACAAGGACCAGGCGCTGGCCGAGATGCACCGCGTGCTGAGGCCGGGCGGCCGCCTG
CTCGTGCTCGAGTTTTCCAAGGTCTGGAAGCCGCTCGAGGCCGCCTACGACCTCTACTCGTTCAAGCTGCTTCCGCTCAT
GGGCAGGCTCGTCGCGAACGACGCCGCGAGCTATCAATATCTCGCCGAGTCGATCCGCATGCATCCCGGCCAGGAAGAAC
TCAAGGCGATGATGGAAAGCGCGGGATTCGCCCGCGTCAGCTACCACAACATGACGGCCGGCGTCGTCGCCCTGCACAAA
GGCTACAAGGTTTGA

Upstream 100 bases:

>100_bases
TGACGGCAGTATGACTGATTTATGACGTCACGCGCCGCGGCGGGGCGTTCCCGCGCCCGCGCTGGTAGAATCGCGGGCTT
GTCCGCTCAGCCCGACTGCC

Downstream 100 bases:

>100_bases
TCGCTGCAGGCCTCGTCGAACGCAGCCTCAATCATCTGCTGCGTCAGTCGCCGGGCGCGCCCGCGGCCCTCGCCCGCCAT
GCCGGCGCGGGCGTGCGCTT

Product: 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase / demethylmenaquinone methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MEKTTHFGYQEVAESEKAAKVAEVFHSVAAQYDIMNDLMSAGLHRLWKRFAVAQAGLRPGMKVLDVAGGTADLTRLFLKE
VGASGTVLLTDINFSMLREGRDRMLNEGRTPPAVQCDGERLPFPDDYFDCVSVAFGLRNMTHKDQALAEMHRVLRPGGRL
LVLEFSKVWKPLEAAYDLYSFKLLPLMGRLVANDAASYQYLAESIRMHPGQEELKAMMESAGFARVSYHNMTAGVVALHK
GYKV

Sequences:

>Translated_244_residues
MEKTTHFGYQEVAESEKAAKVAEVFHSVAAQYDIMNDLMSAGLHRLWKRFAVAQAGLRPGMKVLDVAGGTADLTRLFLKE
VGASGTVLLTDINFSMLREGRDRMLNEGRTPPAVQCDGERLPFPDDYFDCVSVAFGLRNMTHKDQALAEMHRVLRPGGRL
LVLEFSKVWKPLEAAYDLYSFKLLPLMGRLVANDAASYQYLAESIRMHPGQEELKAMMESAGFARVSYHNMTAGVVALHK
GYKV
>Mature_244_residues
MEKTTHFGYQEVAESEKAAKVAEVFHSVAAQYDIMNDLMSAGLHRLWKRFAVAQAGLRPGMKVLDVAGGTADLTRLFLKE
VGASGTVLLTDINFSMLREGRDRMLNEGRTPPAVQCDGERLPFPDDYFDCVSVAFGLRNMTHKDQALAEMHRVLRPGGRL
LVLEFSKVWKPLEAAYDLYSFKLLPLMGRLVANDAASYQYLAESIRMHPGQEELKAMMESAGFARVSYHNMTAGVVALHK
GYKV

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2)

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family

Homologues:

Organism=Homo sapiens, GI116063536, Length=276, Percent_Identity=45.2898550724638, Blast_Score=243, Evalue=1e-64,
Organism=Escherichia coli, GI48994983, Length=245, Percent_Identity=62.0408163265306, Blast_Score=316, Evalue=8e-88,
Organism=Caenorhabditis elegans, GI17557039, Length=245, Percent_Identity=43.6734693877551, Blast_Score=216, Evalue=6e-57,
Organism=Saccharomyces cerevisiae, GI6323526, Length=261, Percent_Identity=42.5287356321839, Blast_Score=191, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24641702, Length=259, Percent_Identity=48.2625482625483, Blast_Score=241, Evalue=4e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIE_THIDA (Q3SM81)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_313973.1
- ProteinModelPortal:   Q3SM81
- STRING:   Q3SM81
- GeneID:   3672862
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0215
- NMPDR:   fig|292415.3.peg.243
- eggNOG:   COG2226
- HOGENOM:   HBG559580
- OMA:   FDVYTIA
- PhylomeDB:   Q3SM81
- BioCyc:   TDEN292415:TBD_0215-MONOMER
- HAMAP:   MF_01813
- InterPro:   IPR004033
- PANTHER:   PTHR10108:SF24
- TIGRFAMs:   TIGR01934

Pfam domain/function: PF01209 Ubie_methyltran

EC number: 2.1.1.-

Molecular weight: Translated: 27190; Mature: 27190

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS01183 UBIE_1; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCC
>Mature Secondary Structure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CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA