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The map label for this gene is aroK

Identifier: 74316225

GI number: 74316225

Start: 225625

End: 226176

Strand: Direct

Name: aroK

Synonym: Tbd_0207

Alternate gene names: 74316225

Gene position: 225625-226176 (Clockwise)

Preceding gene: 74316224

Following gene: 74316226

Centisome position: 7.75

GC content: 65.4

Gene sequence:

>552_bases
ATGAGCAAGCGCGACAATCTTTACCTCGTGGGCCTGATGGGCGCCGGCAAGACGACGGTCGGGCGCCTGCTCGCGAAGCA
CTACGGCTGCACGTTCTACGATTCCGACCACGAGATCGAAGCGCGCACGGGCGTGAAGATTCCCGTCATATTCGAGATCG
AGGGCGAGGCCGGCTTCCGCAGGCGCGAGGAAGCGGTCATCGCTGAACTCACGACGCTTTCCGGAATCGTCCTCGCGACT
GGCGGCGGCGCCGTCCTGTCGCCTGCCAACCGCGAACACCTGCGCACCAACGGCCTGGTGATCTACCTGCGCGGATCGCC
CGAACAGCTGTGCGAGCGGACTCGGAACGATCGCAATCGGCCGCTGCTGCAAACCGGGAACCCGCTCGCCAAGCTGCGCG
AGCTGTATCAGCAGCGCGATCCGATCTATCGCGAGCTCGCCGACGTGACGGTCGACACTGCGCGTCAAAGCGTCGCCGGA
ATGACGCGCGTGCTTTACGGCAAGCTCGACCTGCTCAAAGGGGAGGCGACGTCGTTCGACCCGGCGGGCTGA

Upstream 100 bases:

>100_bases
ATGCAGGAACAAAAAACGCGGGCTTGCCCGCGTTTTTTGTTGTCGTTCAGTCCGACCACGCGCGCGCACCGCCGTCTCCA
ACCTCAATTAGAATGGCGCC

Downstream 100 bases:

>100_bases
GGCGGGGCGCCGGCCCGGCCTGGCTGTCGGCGGGCGGCTCGCAAGCTGCCCAGCGGCCTGCGCATCGCTTCGTCTACACG
CACGGCGCAGCACGTATCCC

Product: shikimate kinase

Products: NA

Alternate protein names: SK

Number of amino acids: Translated: 183; Mature: 182

Protein sequence:

>183_residues
MSKRDNLYLVGLMGAGKTTVGRLLAKHYGCTFYDSDHEIEARTGVKIPVIFEIEGEAGFRRREEAVIAELTTLSGIVLAT
GGGAVLSPANREHLRTNGLVIYLRGSPEQLCERTRNDRNRPLLQTGNPLAKLRELYQQRDPIYRELADVTVDTARQSVAG
MTRVLYGKLDLLKGEATSFDPAG

Sequences:

>Translated_183_residues
MSKRDNLYLVGLMGAGKTTVGRLLAKHYGCTFYDSDHEIEARTGVKIPVIFEIEGEAGFRRREEAVIAELTTLSGIVLAT
GGGAVLSPANREHLRTNGLVIYLRGSPEQLCERTRNDRNRPLLQTGNPLAKLRELYQQRDPIYRELADVTVDTARQSVAG
MTRVLYGKLDLLKGEATSFDPAG
>Mature_182_residues
SKRDNLYLVGLMGAGKTTVGRLLAKHYGCTFYDSDHEIEARTGVKIPVIFEIEGEAGFRRREEAVIAELTTLSGIVLATG
GGAVLSPANREHLRTNGLVIYLRGSPEQLCERTRNDRNRPLLQTGNPLAKLRELYQQRDPIYRELADVTVDTARQSVAGM
TRVLYGKLDLLKGEATSFDPAG

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family

Homologues:

Organism=Escherichia coli, GI87082255, Length=158, Percent_Identity=50.6329113924051, Blast_Score=157, Evalue=5e-40,
Organism=Escherichia coli, GI1786587, Length=154, Percent_Identity=37.6623376623377, Blast_Score=98, Evalue=4e-22,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): AROK_THIDA (Q3SM89)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_313965.1
- ProteinModelPortal:   Q3SM89
- SMR:   Q3SM89
- STRING:   Q3SM89
- GeneID:   3673653
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0207
- NMPDR:   fig|292415.3.peg.194
- eggNOG:   COG0703
- HOGENOM:   HBG335815
- OMA:   VIYLRAN
- PhylomeDB:   Q3SM89
- BioCyc:   TDEN292415:TBD_0207-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00109
- InterPro:   IPR000623
- InterPro:   IPR023000
- PRINTS:   PR01100

Pfam domain/function: PF01202 SKI

EC number: =2.7.1.71

Molecular weight: Translated: 20190; Mature: 20059

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: BINDING 36-36 BINDING 60-60 BINDING 82-82 BINDING 120-120 BINDING 139-139 BINDING 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRDNLYLVGLMGAGKTTVGRLLAKHYGCTFYDSDHEIEARTGVKIPVIFEIEGEAGFR
CCCCCCEEEEEEECCCHHHHHHHHHHHCCCEEECCCCCEECCCCCEEEEEEEECCCCCCH
RREEAVIAELTTLSGIVLATGGGAVLSPANREHLRTNGLVIYLRGSPEQLCERTRNDRNR
HHHHHHHHHHHHHCEEEEEECCCEEECCCCHHHEECCCEEEEEECCHHHHHHHHCCCCCC
PLLQTGNPLAKLRELYQQRDPIYRELADVTVDTARQSVAGMTRVLYGKLDLLKGEATSFD
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PAG
CCC
>Mature Secondary Structure 
SKRDNLYLVGLMGAGKTTVGRLLAKHYGCTFYDSDHEIEARTGVKIPVIFEIEGEAGFR
CCCCCEEEEEEECCCHHHHHHHHHHHCCCEEECCCCCEECCCCCEEEEEEEECCCCCCH
RREEAVIAELTTLSGIVLATGGGAVLSPANREHLRTNGLVIYLRGSPEQLCERTRNDRNR
HHHHHHHHHHHHHCEEEEEECCCEEECCCCHHHEECCCEEEEEECCHHHHHHHHCCCCCC
PLLQTGNPLAKLRELYQQRDPIYRELADVTVDTARQSVAGMTRVLYGKLDLLKGEATSFD
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PAG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA