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The map label for this gene is pilQ [H]

Identifier: 74316224

GI number: 74316224

Start: 223282

End: 225519

Strand: Direct

Name: pilQ [H]

Synonym: Tbd_0206

Alternate gene names: 74316224

Gene position: 223282-225519 (Clockwise)

Preceding gene: 74316223

Following gene: 74316225

Centisome position: 7.67

GC content: 63.49

Gene sequence:

>2238_bases
ATGAACGCACTGCCGAAAATTGCCCGGAAAATGGCCCATCGCCTGATCGCGGTCTTCCTATTCACCGGCCTGGCCATGGG
CCCCGCGGCGCAGGCCGCCGACGCGCCGCCCACCGCCAACGCGGTGCAAAGCGTCGAAACGACGACCTTGCCCGGCGGCA
AGGTGGTGGTCCGTGTCAACCTCAAGAAGCCGCTCGCCGCGACACCGGCCGGCTTTACGGTCGGCAATCCGCCGCGGATC
GCACTCGATCTGCCCGATACCGGCAACGCGATGGGGCGCAACACGGTCGACGCCAATCTCGGCCCCTTGTCGAACGTGAA
CGTCGTGCAGGCGGGGACGCGCACCCGGCTTGTGCTGAACCTGAACAAGTCCGTCGAATATGACGCGCGGATCGAAGGCA
ATAGCCTGCTCGTCGCGCTGGGCGACGTCGGTGCGGGCGCCACGCCCGTGAATGCGAGCCCGCGATTTGCCGAGGCCGCA
CCTGGCGAGTCGCGTCACACGATCCGCGACGTCGACTTCCGCCGCGGCGCGGCCGGCGAAGCGCGCATCGTGACGACGCT
GTCGGACGCACAGGCGGGCATCAACATCCGCCAGCAGGCCAACGGCGTCACCGTCGACTTCATCGGAACCGAATTGCCGA
AAGCCTTGCAGCGTCGTCTCGACGTCGCCGACTTCGGTACGCCGGTGAAAGTGATCGAGACCTATTCGTTGGGCGACAAC
ACGCGGATGGTGATCCAGCCGCAGGGCGGCTGGGAATACTCGGCCTATCAGACCGATAACAGCTTCATCGTCGAGGTCAA
GGCGAGCGACGACGCGCAGAAGAAAACCGCCGACGGGAAGGTGAAATATAGCGGCGAGAAACTGTCGCTTAACTTCCAGA
ATGTCGAAGTGCGCTCGGTGCTGCAGGTGATCGCCGACTTCACCGGCCTCAACATCGTCGCGAGCGATACGGTCACCGGC
AACCTCACGCTGCGACTCAAAGACGTCCCGTGGGACCAGGCGCTCGACCTGATCATGCAGACCAAGGGTCTCGACAAGCG
CCAGAACGGCAACGTCATCTGGATCGCGCCGCGCGATGAGTTGCTGGCCAAGGAAAAGCTCGAATTCGAAGCCAAGAAAC
AGGTGGCCGACCTCGAGCCCCTGGTCACCGACTACATCCAGCTCAACTATCTCCGCGCTGACGAGGCGCAGACGATGTTG
TACGGCTTCGCCTCGGGTGCGTTCCTGAACTCCGCGGGCAACAACGCGGTGAACTGCTCCACGCAGGCCCAGGGCCTCGG
CGGCGCGGCGGTCGCGCAGGCCATGCAAAAAGGTGAAGGCGACACCGATCAGAAGGTGCTAACGAAGCGCGGGCGTGCGA
CCTACGAACTGAAGACCAATACGCTCATCATCACCGACACCGCGAGCAAGATCGCCGAAGTGCGTGGCCTGCTCGCGCGA
CTCGATGTGCCCGCGCGTCAGGTCATGATCGAAGCGCGTGTCGTCATCGCCGATAGCCTTTGGAGCCGCGAGCTCGGAGT
CCGCCTCGGCACCCGTCTGTTCAAGGCAACCGGACGCGACAATATCGGCGTCGGCGGGACCCTGAACGAATCCGCGTCGA
TCGCAGCCGGTGGAACGCCGACCGTCACGCCGCAGGTGGAGTTGGGCACCGTGGGAGCCGCCGCCGGACAGATCGCGATG
TCCCTGCTCAATGCCAGCAACGGAAACCTGCTCAGCCTGGAGTTGTCGGCGCTCGAAGCGGACAATCGCGGCAAGATTCT
CTCCAACCCGCGCGTGATGACGTCCAACCAGAAGCCCGCCGTGATCCTGAACGGTACGCAAATTCCGTTCATCACACCGG
GTACTGCGAACAGCCCCGCGACCGTGACGTTCAAGGACGCATTCCTGTGTCTGCTCGTCGACCCTCAGATACTGAACAAC
GATGCGCTGATCATGACGATCGAAGTGCAAAAGGATGCGATCGATACCGCCAATGCGGCCGTGGGGGCCGACATCATCGC
CCCGGCGATCGCCACGCGCCGGATCAAGACGCAGGTTCGCGTGAACAACGGCGAGACGCTGGTCCTCGGTGGCATATTCG
ACCAGACCGAGTTCAACAACGTCGACAAGGTGCCCTTCCTCGGCGACGTGCCCGGGCTCGGCAACCTGTTCAAACGGACC
AACAAGCAGGACCAGAAGACCGAGATGATCGTGTTCATCACGCCACGCATTCTCGACGAGCGGCTGTCCCTGCGCTGA

Upstream 100 bases:

>100_bases
TGCGCGAGATCGTGCAGGACAGCGCCGGGGATTGGTCCGAGCGCATCAGCACCCTGATATTGCAAGAATGATTTGTCGCA
TGAATTCCAAAGAGGCTGAC

Downstream 100 bases:

>100_bases
GCGGGATGCAGGAACAAAAAACGCGGGCTTGCCCGCGTTTTTTGTTGTCGTTCAGTCCGACCACGCGCGCGCACCGCCGT
CTCCAACCTCAATTAGAATG

Product: type IV pilus assembly protein PilQ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 745; Mature: 745

Protein sequence:

>745_residues
MNALPKIARKMAHRLIAVFLFTGLAMGPAAQAADAPPTANAVQSVETTTLPGGKVVVRVNLKKPLAATPAGFTVGNPPRI
ALDLPDTGNAMGRNTVDANLGPLSNVNVVQAGTRTRLVLNLNKSVEYDARIEGNSLLVALGDVGAGATPVNASPRFAEAA
PGESRHTIRDVDFRRGAAGEARIVTTLSDAQAGINIRQQANGVTVDFIGTELPKALQRRLDVADFGTPVKVIETYSLGDN
TRMVIQPQGGWEYSAYQTDNSFIVEVKASDDAQKKTADGKVKYSGEKLSLNFQNVEVRSVLQVIADFTGLNIVASDTVTG
NLTLRLKDVPWDQALDLIMQTKGLDKRQNGNVIWIAPRDELLAKEKLEFEAKKQVADLEPLVTDYIQLNYLRADEAQTML
YGFASGAFLNSAGNNAVNCSTQAQGLGGAAVAQAMQKGEGDTDQKVLTKRGRATYELKTNTLIITDTASKIAEVRGLLAR
LDVPARQVMIEARVVIADSLWSRELGVRLGTRLFKATGRDNIGVGGTLNESASIAAGGTPTVTPQVELGTVGAAAGQIAM
SLLNASNGNLLSLELSALEADNRGKILSNPRVMTSNQKPAVILNGTQIPFITPGTANSPATVTFKDAFLCLLVDPQILNN
DALIMTIEVQKDAIDTANAAVGADIIAPAIATRRIKTQVRVNNGETLVLGGIFDQTEFNNVDKVPFLGDVPGLGNLFKRT
NKQDQKTEMIVFITPRILDERLSLR

Sequences:

>Translated_745_residues
MNALPKIARKMAHRLIAVFLFTGLAMGPAAQAADAPPTANAVQSVETTTLPGGKVVVRVNLKKPLAATPAGFTVGNPPRI
ALDLPDTGNAMGRNTVDANLGPLSNVNVVQAGTRTRLVLNLNKSVEYDARIEGNSLLVALGDVGAGATPVNASPRFAEAA
PGESRHTIRDVDFRRGAAGEARIVTTLSDAQAGINIRQQANGVTVDFIGTELPKALQRRLDVADFGTPVKVIETYSLGDN
TRMVIQPQGGWEYSAYQTDNSFIVEVKASDDAQKKTADGKVKYSGEKLSLNFQNVEVRSVLQVIADFTGLNIVASDTVTG
NLTLRLKDVPWDQALDLIMQTKGLDKRQNGNVIWIAPRDELLAKEKLEFEAKKQVADLEPLVTDYIQLNYLRADEAQTML
YGFASGAFLNSAGNNAVNCSTQAQGLGGAAVAQAMQKGEGDTDQKVLTKRGRATYELKTNTLIITDTASKIAEVRGLLAR
LDVPARQVMIEARVVIADSLWSRELGVRLGTRLFKATGRDNIGVGGTLNESASIAAGGTPTVTPQVELGTVGAAAGQIAM
SLLNASNGNLLSLELSALEADNRGKILSNPRVMTSNQKPAVILNGTQIPFITPGTANSPATVTFKDAFLCLLVDPQILNN
DALIMTIEVQKDAIDTANAAVGADIIAPAIATRRIKTQVRVNNGETLVLGGIFDQTEFNNVDKVPFLGDVPGLGNLFKRT
NKQDQKTEMIVFITPRILDERLSLR
>Mature_745_residues
MNALPKIARKMAHRLIAVFLFTGLAMGPAAQAADAPPTANAVQSVETTTLPGGKVVVRVNLKKPLAATPAGFTVGNPPRI
ALDLPDTGNAMGRNTVDANLGPLSNVNVVQAGTRTRLVLNLNKSVEYDARIEGNSLLVALGDVGAGATPVNASPRFAEAA
PGESRHTIRDVDFRRGAAGEARIVTTLSDAQAGINIRQQANGVTVDFIGTELPKALQRRLDVADFGTPVKVIETYSLGDN
TRMVIQPQGGWEYSAYQTDNSFIVEVKASDDAQKKTADGKVKYSGEKLSLNFQNVEVRSVLQVIADFTGLNIVASDTVTG
NLTLRLKDVPWDQALDLIMQTKGLDKRQNGNVIWIAPRDELLAKEKLEFEAKKQVADLEPLVTDYIQLNYLRADEAQTML
YGFASGAFLNSAGNNAVNCSTQAQGLGGAAVAQAMQKGEGDTDQKVLTKRGRATYELKTNTLIITDTASKIAEVRGLLAR
LDVPARQVMIEARVVIADSLWSRELGVRLGTRLFKATGRDNIGVGGTLNESASIAAGGTPTVTPQVELGTVGAAAGQIAM
SLLNASNGNLLSLELSALEADNRGKILSNPRVMTSNQKPAVILNGTQIPFITPGTANSPATVTFKDAFLCLLVDPQILNN
DALIMTIEVQKDAIDTANAAVGADIIAPAIATRRIKTQVRVNNGETLVLGGIFDQTEFNNVDKVPFLGDVPGLGNLFKRT
NKQDQKTEMIVFITPRILDERLSLR

Specific function: Essential for the formation of pili. Involved in the biogenesis of type 4 fimbriae probably by serving as a "porthole" allowing passage of the fimbrae through the outer membrane [H]

COG id: COG4796

COG function: function code U; Type II secretory pathway, component HofQ

Gene ontology:

Cell location: Cell outer membrane (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI1789793, Length=456, Percent_Identity=31.359649122807, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI87082242, Length=295, Percent_Identity=31.1864406779661, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR013355
- InterPro:   IPR011662
- InterPro:   IPR004846
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N; PF07660 STN [H]

EC number: NA

Molecular weight: Translated: 79462; Mature: 79462

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALPKIARKMAHRLIAVFLFTGLAMGPAAQAADAPPTANAVQSVETTTLPGGKVVVRVN
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCEEEECCCCEEEEEEE
LKKPLAATPAGFTVGNPPRIALDLPDTGNAMGRNTVDANLGPLSNVNVVQAGTRTRLVLN
CCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEE
LNKSVEYDARIEGNSLLVALGDVGAGATPVNASPRFAEAAPGESRHTIRDVDFRRGAAGE
ECCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCC
ARIVTTLSDAQAGINIRQQANGVTVDFIGTELPKALQRRLDVADFGTPVKVIETYSLGDN
EEEEEEECCCCCCCEEEECCCCEEEEECCCHHHHHHHHHCCHHHCCCCEEEEEEEECCCC
TRMVIQPQGGWEYSAYQTDNSFIVEVKASDDAQKKTADGKVKYSGEKLSLNFQNVEVRSV
CEEEEEECCCCEEEEEECCCEEEEEEECCCCCHHHCCCCEEEECCCEEEEEECCCHHHHH
LQVIADFTGLNIVASDTVTGNLTLRLKDVPWDQALDLIMQTKGLDKRQNGNVIWIAPRDE
HHHHHCCCCCEEEEECEEEEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEEEECCHH
LLAKEKLEFEAKKQVADLEPLVTDYIQLNYLRADEAQTMLYGFASGAFLNSAGNNAVNCS
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCEEECCCCCEEECC
TQAQGLGGAAVAQAMQKGEGDTDQKVLTKRGRATYELKTNTLIITDTASKIAEVRGLLAR
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCEEEEEECCEEEEECCHHHHHHHHHHHHH
LDVPARQVMIEARVVIADSLWSRELGVRLGTRLFKATGRDNIGVGGTLNESASIAAGGTP
CCCCHHHEEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCC
TVTPQVELGTVGAAAGQIAMSLLNASNGNLLSLELSALEADNRGKILSNPRVMTSNQKPA
CCCCEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEECCCEEEECCCCCE
VILNGTQIPFITPGTANSPATVTFKDAFLCLLVDPQILNNDALIMTIEVQKDAIDTANAA
EEECCCCCCEECCCCCCCCCEEEECCEEEEEEECCHHCCCCEEEEEEEECCCCCCCCCHH
VGADIIAPAIATRRIKTQVRVNNGETLVLGGIFDQTEFNNVDKVPFLGDVPGLGNLFKRT
HCHHHHHHHHHHHEEEEEEEECCCCEEEEECEECCCCCCCCCCCCCCCCCCCCHHHHHHC
NKQDQKTEMIVFITPRILDERLSLR
CCCCCCCCEEEEECHHHHHHHHCCC
>Mature Secondary Structure
MNALPKIARKMAHRLIAVFLFTGLAMGPAAQAADAPPTANAVQSVETTTLPGGKVVVRVN
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCEEEECCCCEEEEEEE
LKKPLAATPAGFTVGNPPRIALDLPDTGNAMGRNTVDANLGPLSNVNVVQAGTRTRLVLN
CCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEE
LNKSVEYDARIEGNSLLVALGDVGAGATPVNASPRFAEAAPGESRHTIRDVDFRRGAAGE
ECCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCC
ARIVTTLSDAQAGINIRQQANGVTVDFIGTELPKALQRRLDVADFGTPVKVIETYSLGDN
EEEEEEECCCCCCCEEEECCCCEEEEECCCHHHHHHHHHCCHHHCCCCEEEEEEEECCCC
TRMVIQPQGGWEYSAYQTDNSFIVEVKASDDAQKKTADGKVKYSGEKLSLNFQNVEVRSV
CEEEEEECCCCEEEEEECCCEEEEEEECCCCCHHHCCCCEEEECCCEEEEEECCCHHHHH
LQVIADFTGLNIVASDTVTGNLTLRLKDVPWDQALDLIMQTKGLDKRQNGNVIWIAPRDE
HHHHHCCCCCEEEEECEEEEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEEEECCHH
LLAKEKLEFEAKKQVADLEPLVTDYIQLNYLRADEAQTMLYGFASGAFLNSAGNNAVNCS
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCEEECCCCCEEECC
TQAQGLGGAAVAQAMQKGEGDTDQKVLTKRGRATYELKTNTLIITDTASKIAEVRGLLAR
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCEEEEEECCEEEEECCHHHHHHHHHHHHH
LDVPARQVMIEARVVIADSLWSRELGVRLGTRLFKATGRDNIGVGGTLNESASIAAGGTP
CCCCHHHEEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCC
TVTPQVELGTVGAAAGQIAMSLLNASNGNLLSLELSALEADNRGKILSNPRVMTSNQKPA
CCCCEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEECCCEEEECCCCCE
VILNGTQIPFITPGTANSPATVTFKDAFLCLLVDPQILNNDALIMTIEVQKDAIDTANAA
EEECCCCCCEECCCCCCCCCEEEECCEEEEEEECCHHCCCCEEEEEEEECCCCCCCCCHH
VGADIIAPAIATRRIKTQVRVNNGETLVLGGIFDQTEFNNVDKVPFLGDVPGLGNLFKRT
HCHHHHHHHHHHHEEEEEEEECCCCEEEEECEECCCCCCCCCCCCCCCCCCCCHHHHHHC
NKQDQKTEMIVFITPRILDERLSLR
CCCCCCCCEEEEECHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7901733; 10984043 [H]