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The map label for this gene is pykA [H]

Identifier: 74316180

GI number: 74316180

Start: 174936

End: 176369

Strand: Direct

Name: pykA [H]

Synonym: Tbd_0162

Alternate gene names: 74316180

Gene position: 174936-176369 (Clockwise)

Preceding gene: 74316179

Following gene: 74316181

Centisome position: 6.01

GC content: 66.18

Gene sequence:

>1434_bases
ATGATTCGCAGAACCAAAATCGTCGCCACCCTCGGCCCCGCCTGCTCCGACGCCAAGACGCTCGATCGGATGATGGAAGC
CGGCCTGGACGTGGTCCGTCTCAACTTCTCCCACGGCGTGGCGCAGGACCACATCGACCGCGCCGAACTCGTGCGTTCGC
TCGCGCGTACGCGCGGTCGCGCGGTCGGCGTCCTCGTCGACCTGCAGGGGCCGAAGATCCGCATCGGCAAATTCAAGGAC
AACAAGATCGTCCTCGCCAAGGGCGACAACTTCATCCTCGACGCGGAATGCGCGCTCGGCGACCAGGAGCGCGTCGGCCT
CGACTACAAGGAATTGCCGAGCGACGTCGCCCGTGGCGTGACGCTCCTGCTCGACGACGGCCGGATCGAACTCTGGGTCG
AAGACGTCAAGGGCAGCGAAATCCACTGCAAGGTCGTGCAGGGCGGCGTGCTCTCGAATAACAAGGGCATCAACCGCAAG
GGCGGCGGTCTTTCGGCCGCCGCGCTGACCGAGAAGGACATCGAGGACATCAAGACCGCCGCGGCGCTCAAGGCCGACTA
CCTCGCGGTCTCCTTCCCGCGCTCGGGCGCCGACATGCGCCAGGCGCGCGAGTTGCTGCGGGCCGCGGGCGGCAGGAGCC
AGCTCGTCGCCAAGATCGAGCGCACTGAAGCGATCGAGAACCTCGAAGAAATCCTCGATGCGTCCGACGCGATCATGGTC
GCCCGCGGCGACCTCGGCGTCGAGGTCGGCGACGCTGCGGTGCCGGCGCTGCAGAAGCGCATGATCCGTATGGCGATCGC
GCGCAACAAGGTCGTCATCACCGCGACGCAGATGATGGAGTCGATGATCACGAGCCCGATCCCGACCCGCGCCGAAGTCT
CCGACGTCGCCAACGCCGTGCTCGACGGCACCGACGCCGTCATGCTCTCGGCCGAGACCGCGGCCGGGCAATACCCGGTC
GAAGCCGTGCAGGCGATGGACCGCGTCTGCCTCGAAGCCGAAAAGGAAGCCGAGACGCAGTTCACGCGCGAGCGGCTCGA
CCACAGCTTCCTGCGCGCCGACGAGGCGATCGCGATGTCGGCCGTGTTCACCTCGGTGCACCTCAACATCAAGGCCATCG
CCGCGCTGACCGAGTCCGGCAATACCTGTCTGTGGATGAGCCGCCTGTCGACGCGCGTGCCGATCTACGCCTTGACACCT
CAGGTCGAAACCCGTAGAAAAGTCACTCTCTTCCGCGGTGTCTATCCGATCAACCTCAAGACCGCCACCAAGGAGCGCGA
CGTGCTGCTCGCCGAGGCCGAGGAAGAATTGCGGCGGCGCGGCGCCGTGCGCGACGGCGACCTGATCCTGCTGACGATCG
GCGAGCCGATCGGCCAGTCGGGCGGCACCAATACGATGAAGATCGTCCGTGTGGGCGCTTCGAAGAAACCCTGA

Upstream 100 bases:

>100_bases
CGCGCGCAAGGCTAAGCGCCTTACGGCAAGACGCGAGGGGGAGGCTGAGGCTTCCCCCTCGGGCTTTCCGCCTTCCCTCC
CTCAACGCTCCACCACAATA

Downstream 100 bases:

>100_bases
ATCTGAAGCACGTTTTTTACCTAGGAGAGTGTAAATGGCCCTGATTTCCCTTCGTCAACTGCTCGACCATGCCGCCGAAA
ACGGCTACGGTCTGCCCGCG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-2 [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MIRRTKIVATLGPACSDAKTLDRMMEAGLDVVRLNFSHGVAQDHIDRAELVRSLARTRGRAVGVLVDLQGPKIRIGKFKD
NKIVLAKGDNFILDAECALGDQERVGLDYKELPSDVARGVTLLLDDGRIELWVEDVKGSEIHCKVVQGGVLSNNKGINRK
GGGLSAAALTEKDIEDIKTAAALKADYLAVSFPRSGADMRQARELLRAAGGRSQLVAKIERTEAIENLEEILDASDAIMV
ARGDLGVEVGDAAVPALQKRMIRMAIARNKVVITATQMMESMITSPIPTRAEVSDVANAVLDGTDAVMLSAETAAGQYPV
EAVQAMDRVCLEAEKEAETQFTRERLDHSFLRADEAIAMSAVFTSVHLNIKAIAALTESGNTCLWMSRLSTRVPIYALTP
QVETRRKVTLFRGVYPINLKTATKERDVLLAEAEEELRRRGAVRDGDLILLTIGEPIGQSGGTNTMKIVRVGASKKP

Sequences:

>Translated_477_residues
MIRRTKIVATLGPACSDAKTLDRMMEAGLDVVRLNFSHGVAQDHIDRAELVRSLARTRGRAVGVLVDLQGPKIRIGKFKD
NKIVLAKGDNFILDAECALGDQERVGLDYKELPSDVARGVTLLLDDGRIELWVEDVKGSEIHCKVVQGGVLSNNKGINRK
GGGLSAAALTEKDIEDIKTAAALKADYLAVSFPRSGADMRQARELLRAAGGRSQLVAKIERTEAIENLEEILDASDAIMV
ARGDLGVEVGDAAVPALQKRMIRMAIARNKVVITATQMMESMITSPIPTRAEVSDVANAVLDGTDAVMLSAETAAGQYPV
EAVQAMDRVCLEAEKEAETQFTRERLDHSFLRADEAIAMSAVFTSVHLNIKAIAALTESGNTCLWMSRLSTRVPIYALTP
QVETRRKVTLFRGVYPINLKTATKERDVLLAEAEEELRRRGAVRDGDLILLTIGEPIGQSGGTNTMKIVRVGASKKP
>Mature_477_residues
MIRRTKIVATLGPACSDAKTLDRMMEAGLDVVRLNFSHGVAQDHIDRAELVRSLARTRGRAVGVLVDLQGPKIRIGKFKD
NKIVLAKGDNFILDAECALGDQERVGLDYKELPSDVARGVTLLLDDGRIELWVEDVKGSEIHCKVVQGGVLSNNKGINRK
GGGLSAAALTEKDIEDIKTAAALKADYLAVSFPRSGADMRQARELLRAAGGRSQLVAKIERTEAIENLEEILDASDAIMV
ARGDLGVEVGDAAVPALQKRMIRMAIARNKVVITATQMMESMITSPIPTRAEVSDVANAVLDGTDAVMLSAETAAGQYPV
EAVQAMDRVCLEAEKEAETQFTRERLDHSFLRADEAIAMSAVFTSVHLNIKAIAALTESGNTCLWMSRLSTRVPIYALTP
QVETRRKVTLFRGVYPINLKTATKERDVLLAEAEEELRRRGAVRDGDLILLTIGEPIGQSGGTNTMKIVRVGASKKP

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=497, Percent_Identity=37.625754527163, Blast_Score=287, Evalue=1e-77,
Organism=Homo sapiens, GI33286420, Length=497, Percent_Identity=37.625754527163, Blast_Score=287, Evalue=1e-77,
Organism=Homo sapiens, GI33286418, Length=489, Percent_Identity=37.8323108384458, Blast_Score=282, Evalue=4e-76,
Organism=Homo sapiens, GI32967597, Length=493, Percent_Identity=35.4969574036511, Blast_Score=265, Evalue=8e-71,
Organism=Homo sapiens, GI10835121, Length=489, Percent_Identity=35.9918200408998, Blast_Score=265, Evalue=8e-71,
Organism=Homo sapiens, GI310128732, Length=291, Percent_Identity=35.7388316151203, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI310128730, Length=291, Percent_Identity=35.7388316151203, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI310128736, Length=239, Percent_Identity=32.6359832635983, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI310128734, Length=239, Percent_Identity=32.6359832635983, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI310128738, Length=218, Percent_Identity=31.651376146789, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1788160, Length=476, Percent_Identity=53.781512605042, Blast_Score=475, Evalue=1e-135,
Organism=Escherichia coli, GI1787965, Length=470, Percent_Identity=38.7234042553192, Blast_Score=306, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI17544584, Length=486, Percent_Identity=36.6255144032922, Blast_Score=291, Evalue=7e-79,
Organism=Caenorhabditis elegans, GI71984413, Length=488, Percent_Identity=34.6311475409836, Blast_Score=256, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI17506829, Length=488, Percent_Identity=34.6311475409836, Blast_Score=256, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI71984406, Length=488, Percent_Identity=34.6311475409836, Blast_Score=256, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI17506831, Length=488, Percent_Identity=34.6311475409836, Blast_Score=256, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6324923, Length=485, Percent_Identity=32.7835051546392, Blast_Score=237, Evalue=4e-63,
Organism=Saccharomyces cerevisiae, GI6319279, Length=486, Percent_Identity=33.5390946502058, Blast_Score=236, Evalue=7e-63,
Organism=Drosophila melanogaster, GI28571814, Length=493, Percent_Identity=36.3083164300203, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24648964, Length=493, Percent_Identity=36.3083164300203, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24648966, Length=410, Percent_Identity=37.3170731707317, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24581235, Length=487, Percent_Identity=32.6488706365503, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24646914, Length=271, Percent_Identity=38.7453874538745, Blast_Score=162, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51705; Mature: 51705

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRRTKIVATLGPACSDAKTLDRMMEAGLDVVRLNFSHGVAQDHIDRAELVRSLARTRGR
CCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHCCHHHHHHHHHHCCCC
AVGVLVDLQGPKIRIGKFKDNKIVLAKGDNFILDAECALGDQERVGLDYKELPSDVARGV
EEEEEEECCCCEEEEEEECCCEEEEEECCCEEEECHHCCCCHHHCCCCHHHCCHHHHCCE
TLLLDDGRIELWVEDVKGSEIHCKVVQGGVLSNNKGINRKGGGLSAAALTEKDIEDIKTA
EEEEECCEEEEEEEECCCCEEEEEEEECCEEECCCCCCCCCCCCEEHHHHHHHHHHHHHH
AALKADYLAVSFPRSGADMRQARELLRAAGGRSQLVAKIERTEAIENLEEILDASDAIMV
HHHHHCEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
ARGDLGVEVGDAAVPALQKRMIRMAIARNKVVITATQMMESMITSPIPTRAEVSDVANAV
EECCCCEEECCHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHHHH
LDGTDAVMLSAETAAGQYPVEAVQAMDRVCLEAEKEAETQFTRERLDHSFLRADEAIAMS
HCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVFTSVHLNIKAIAALTESGNTCLWMSRLSTRVPIYALTPQVETRRKVTLFRGVYPINLK
HHHHHHEEEEEEEEEEECCCCEEEEEEHHCCCCCEEEECCCCCCCCEEEEEECEEEEEEE
TATKERDVLLAEAEEELRRRGAVRDGDLILLTIGEPIGQSGGTNTMKIVRVGASKKP
ECCCCCCEEEHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCC
>Mature Secondary Structure
MIRRTKIVATLGPACSDAKTLDRMMEAGLDVVRLNFSHGVAQDHIDRAELVRSLARTRGR
CCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHCCHHHHHHHHHHCCCC
AVGVLVDLQGPKIRIGKFKDNKIVLAKGDNFILDAECALGDQERVGLDYKELPSDVARGV
EEEEEEECCCCEEEEEEECCCEEEEEECCCEEEECHHCCCCHHHCCCCHHHCCHHHHCCE
TLLLDDGRIELWVEDVKGSEIHCKVVQGGVLSNNKGINRKGGGLSAAALTEKDIEDIKTA
EEEEECCEEEEEEEECCCCEEEEEEEECCEEECCCCCCCCCCCCEEHHHHHHHHHHHHHH
AALKADYLAVSFPRSGADMRQARELLRAAGGRSQLVAKIERTEAIENLEEILDASDAIMV
HHHHHCEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
ARGDLGVEVGDAAVPALQKRMIRMAIARNKVVITATQMMESMITSPIPTRAEVSDVANAV
EECCCCEEECCHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHHHH
LDGTDAVMLSAETAAGQYPVEAVQAMDRVCLEAEKEAETQFTRERLDHSFLRADEAIAMS
HCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVFTSVHLNIKAIAALTESGNTCLWMSRLSTRVPIYALTPQVETRRKVTLFRGVYPINLK
HHHHHHEEEEEEEEEEECCCCEEEEEEHHCCCCCEEEECCCCCCCCEEEEEECEEEEEEE
TATKERDVLLAEAEEELRRRGAVRDGDLILLTIGEPIGQSGGTNTMKIVRVGASKKP
ECCCCCCEEEHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1732206; 1859631; 8439398 [H]