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The map label for this gene is pgk

Identifier: 74316179

GI number: 74316179

Start: 173669

End: 174850

Strand: Direct

Name: pgk

Synonym: Tbd_0161

Alternate gene names: 74316179

Gene position: 173669-174850 (Clockwise)

Preceding gene: 74316178

Following gene: 74316180

Centisome position: 5.97

GC content: 65.23

Gene sequence:

>1182_bases
ATGGCATTCATCCGCGTCACCGATCTCGATCTGGCGGGCAAGCGCGTCTTCATCCGCGCCGACATGAACGTGCCCGTCAA
GGACGGCAAGGTCACGTCCGACGCGCGCATCACCGCCTCGATGCGCACCATCGAGCACTGCATGAGGGCCGGCGCCAAGG
TCATGGTGACCTCGCACCTCGGCCGCCCGACCGAAGGCGAGTTCAAGGAAGAGGATTCGCTGAAGCCCGTCGTCGACGTG
ATCGCGCAGAAGCTCGGCAAAAACGTGCGCCTGATCCGTGAATGGACCGAGGGCGGTTTCGACGTCGCGAACGGCGAACT
CGTCGTGCTCGAGAACTGCCGCGTCAACAAGGGCGAGAAGAAGAACGTCGACGAGACCGCGAAGAAATACGCCGCGCTGT
GCGACGTCTTCGTGATGGACGCCTTCGGCACCGCGCACCGCGCCGAAGCCTCGACCCACGGCATCGCCAAATTCGCGCCG
ACCGCTGCCGCCGGCATTCTGCTCACCGAAGAGCTCGACGCGCTGGCCAAGGCGCTCGCCAACCCGGCGCGGCCGATGGT
CGCGATCGTCGGCGGCTCCAAGGTCTCGACCAAGCTGACCGTGCTCGAGGCGCTGTCCGAGAAGGTCGACCAGTTGGTCG
TCGGCGGCGGCATCGCCAACACCTTCCTCGCCGCGATGGGCAAGAACGTCGGCAAGTCGCTGTGTGAACACGACCTGATC
CCGACTGCCAAGACGCTGATCGAGAAGATGGCCGCGCGCGGCGCCTCGATCCCGATCGCCGTCGACGTGGTGTGCGGCAA
GAAGTTCGACGCCAACGAGCCCGCCGTGCTGAAGAGCGCCGACGAGGTGGCCGACGACGACATGATCTTCGACATCGGCC
CGAAGAGCGCGCAGGAGCTTGCCGACATCATCGCCAAGGCCGGCACGATTGTGTGGAACGGCCCGGTCGGCGTGTTCGAG
TTCGACCAGTTCGGCGAAGGCACCAAGACCGTCTCGATGGCGATCGCCAACGCCCCGGGCTTCAGCCTGGCCGGCGGCGG
CGACACCATCGCCGCGATCCAGAAATACGACATCTACGACAAAGTGAGCTATATCTCGACCGCCGGCGGCGCCTTCCTCG
AATACCTGGAAGGCAAGGTGTTGCCGGCCGTGGCGATTCTGGAGGAACGCGCGCAAGGCTAA

Upstream 100 bases:

>100_bases
CCTGAATCCCGACCCCTGACCTTCAGCAGTGAGCCTTAAGGCCTGGCGCGGTTTCCAACCCCGCGCCAACCCTTAAACCT
TACTTATCAGGAGAACACCC

Downstream 100 bases:

>100_bases
GCGCCTTACGGCAAGACGCGAGGGGGAGGCTGAGGCTTCCCCCTCGGGCTTTCCGCCTTCCCTCCCTCAACGCTCCACCA
CAATAATGATTCGCAGAACC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MAFIRVTDLDLAGKRVFIRADMNVPVKDGKVTSDARITASMRTIEHCMRAGAKVMVTSHLGRPTEGEFKEEDSLKPVVDV
IAQKLGKNVRLIREWTEGGFDVANGELVVLENCRVNKGEKKNVDETAKKYAALCDVFVMDAFGTAHRAEASTHGIAKFAP
TAAAGILLTEELDALAKALANPARPMVAIVGGSKVSTKLTVLEALSEKVDQLVVGGGIANTFLAAMGKNVGKSLCEHDLI
PTAKTLIEKMAARGASIPIAVDVVCGKKFDANEPAVLKSADEVADDDMIFDIGPKSAQELADIIAKAGTIVWNGPVGVFE
FDQFGEGTKTVSMAIANAPGFSLAGGGDTIAAIQKYDIYDKVSYISTAGGAFLEYLEGKVLPAVAILEERAQG

Sequences:

>Translated_393_residues
MAFIRVTDLDLAGKRVFIRADMNVPVKDGKVTSDARITASMRTIEHCMRAGAKVMVTSHLGRPTEGEFKEEDSLKPVVDV
IAQKLGKNVRLIREWTEGGFDVANGELVVLENCRVNKGEKKNVDETAKKYAALCDVFVMDAFGTAHRAEASTHGIAKFAP
TAAAGILLTEELDALAKALANPARPMVAIVGGSKVSTKLTVLEALSEKVDQLVVGGGIANTFLAAMGKNVGKSLCEHDLI
PTAKTLIEKMAARGASIPIAVDVVCGKKFDANEPAVLKSADEVADDDMIFDIGPKSAQELADIIAKAGTIVWNGPVGVFE
FDQFGEGTKTVSMAIANAPGFSLAGGGDTIAAIQKYDIYDKVSYISTAGGAFLEYLEGKVLPAVAILEERAQG
>Mature_392_residues
AFIRVTDLDLAGKRVFIRADMNVPVKDGKVTSDARITASMRTIEHCMRAGAKVMVTSHLGRPTEGEFKEEDSLKPVVDVI
AQKLGKNVRLIREWTEGGFDVANGELVVLENCRVNKGEKKNVDETAKKYAALCDVFVMDAFGTAHRAEASTHGIAKFAPT
AAAGILLTEELDALAKALANPARPMVAIVGGSKVSTKLTVLEALSEKVDQLVVGGGIANTFLAAMGKNVGKSLCEHDLIP
TAKTLIEKMAARGASIPIAVDVVCGKKFDANEPAVLKSADEVADDDMIFDIGPKSAQELADIIAKAGTIVWNGPVGVFEF
DQFGEGTKTVSMAIANAPGFSLAGGGDTIAAIQKYDIYDKVSYISTAGGAFLEYLEGKVLPAVAILEERAQG

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI31543397, Length=405, Percent_Identity=40, Blast_Score=267, Evalue=2e-71,
Organism=Homo sapiens, GI4505763, Length=405, Percent_Identity=38.7654320987654, Blast_Score=256, Evalue=3e-68,
Organism=Escherichia coli, GI1789294, Length=392, Percent_Identity=66.0714285714286, Blast_Score=501, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17508823, Length=410, Percent_Identity=42.4390243902439, Blast_Score=287, Evalue=5e-78,
Organism=Saccharomyces cerevisiae, GI6319857, Length=417, Percent_Identity=39.0887290167866, Blast_Score=262, Evalue=7e-71,
Organism=Drosophila melanogaster, GI17136394, Length=416, Percent_Identity=39.6634615384615, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24581203, Length=414, Percent_Identity=36.4734299516908, Blast_Score=234, Evalue=9e-62,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_THIDA (Q3SMD2)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_313919.1
- ProteinModelPortal:   Q3SMD2
- SMR:   Q3SMD2
- STRING:   Q3SMD2
- GeneID:   3673607
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0161
- NMPDR:   fig|292415.3.peg.71
- eggNOG:   COG0126
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- PhylomeDB:   Q3SMD2
- ProtClustDB:   PRK00073
- BioCyc:   TDEN292415:TBD_0161-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 41556; Mature: 41425

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 114-114 BINDING 147-147 BINDING 198-198 BINDING 320-320

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFIRVTDLDLAGKRVFIRADMNVPVKDGKVTSDARITASMRTIEHCMRAGAKVMVTSHL
CEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECC
GRPTEGEFKEEDSLKPVVDVIAQKLGKNVRLIREWTEGGFDVANGELVVLENCRVNKGEK
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEHHHHHCCCCEECCCCEEEEECCCCCCCCC
KNVDETAKKYAALCDVFVMDAFGTAHRAEASTHGIAKFAPTAAAGILLTEELDALAKALA
CCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHC
NPARPMVAIVGGSKVSTKLTVLEALSEKVDQLVVGGGIANTFLAAMGKNVGKSLCEHDLI
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHCCC
PTAKTLIEKMAARGASIPIAVDVVCGKKFDANEPAVLKSADEVADDDMIFDIGPKSAQEL
CHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHCCHHHHCCCCEEEECCCCCHHHH
ADIIAKAGTIVWNGPVGVFEFDQFGEGTKTVSMAIANAPGFSLAGGGDTIAAIQKYDIYD
HHHHHHCCEEEECCCCCEEECHHCCCCCCEEEEEEECCCCCEECCCCCHHHHHHHHCHHH
KVSYISTAGGAFLEYLEGKVLPAVAILEERAQG
HHHHHHHCHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
AFIRVTDLDLAGKRVFIRADMNVPVKDGKVTSDARITASMRTIEHCMRAGAKVMVTSHL
EEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECC
GRPTEGEFKEEDSLKPVVDVIAQKLGKNVRLIREWTEGGFDVANGELVVLENCRVNKGEK
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEHHHHHCCCCEECCCCEEEEECCCCCCCCC
KNVDETAKKYAALCDVFVMDAFGTAHRAEASTHGIAKFAPTAAAGILLTEELDALAKALA
CCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHC
NPARPMVAIVGGSKVSTKLTVLEALSEKVDQLVVGGGIANTFLAAMGKNVGKSLCEHDLI
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHCCC
PTAKTLIEKMAARGASIPIAVDVVCGKKFDANEPAVLKSADEVADDDMIFDIGPKSAQEL
CHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHCCHHHHCCCCEEEECCCCCHHHH
ADIIAKAGTIVWNGPVGVFEFDQFGEGTKTVSMAIANAPGFSLAGGGDTIAAIQKYDIYD
HHHHHHCCEEEECCCCCEEECHHCCCCCCEEEEEEECCCCCEECCCCCHHHHHHHHCHHH
KVSYISTAGGAFLEYLEGKVLPAVAILEERAQG
HHHHHHHCHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA