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The map label for this gene is glcD [H]

Identifier: 74316065

GI number: 74316065

Start: 47677

End: 49128

Strand: Reverse

Name: glcD [H]

Synonym: Tbd_0047

Alternate gene names: 74316065

Gene position: 49128-47677 (Counterclockwise)

Preceding gene: 74316066

Following gene: 74316064

Centisome position: 1.69

GC content: 69.77

Gene sequence:

>1452_bases
ATGCTCAGTCCCAGCTTCCTCGCGCATCTGCGCACTCTCCTGCCGGCGCACTGCGTGCTCAACGAGCGCGAAGACCTGCG
GCCCTTCGAGTCCGACGGCCTTGCCGCCTACCGCAACACGCCCGACGTCGTCGTGCTGCCGGAGACCGAGGCGCAGGTCG
CGGCGATCCTCGAGCTGTGCCACGCGCACCGGGTCGCGGTGGTCGCGCGCGGCGCCGGCACCGGGCTGTCGGCCGGCGCG
CTGCCCGTCGACGGCGCCGTGCTGCTGGTGCTGGCGAAGCTCAACCGCATCAAGGCGATCGACCCGCTCGCGCGCACCGC
GGTCGTCGAGCCTGGCGTGCGCAACCTCGCGATTTCCGAGGCGGCCGCCGCCTACAACCTGTATTACGCGCCCGATCCGT
CGTCGCAGATCGCGTGCTCGATCGGCGGCAACGTCGCGGAAAATTCCGGCGGCGTGCATTGCCTCAAATATGGCCTCACG
GTGCACAACATCGCGAAGCTGCGCGCCTGGACCGTCGACGGCAAGCTGCTCGAAATCGGCAGCGACGCGCTTGACGCGCC
GGGCTACGACCTGCTCGCGCTCCTGACCGGCTCGGAAGGCATGCTCGCCGTCATCACCGAAGTCACGGTCAAGCTGCTGC
CGCGTCCCGAGCGCGCCCAGGTCGTCCTCGCGGCCTTCGACGACGTCGCCAAGGCCGGCGCGGCCGTCGGCAACATCATC
GCCGCGGGCGTCGTCCCGGCCGGACTCGAGATGATGGACCGCCTCGCGATCCAGGCCGCCGAGGCCTTCGTCCACGCCGG
CTACCCAATGGCCGAGGCACTGCTCCTGTGCGAGGTCGACGGCGTCAACGAAGAAGTCTCGGCCGACATCTACACCGTGC
GCCAGGTGCTCGAAGCCTCCGGCGCGACCGAGATCCGCACCGCCGACAGCGAGGAACAACGGCTGCGCTTCTGGGCCGGC
CGCAAGGCCGCGTTTCCCGCCGTCGGGCGGCTCGCACCCGACTACTACTGCATGGACGGCACGATTCCGCGTGCGCAACT
GCCCCACGTCCTGCAGCGCATCGGCGCGCTGAGCGCCGAGTTCGGACTCGCGGTCGCCAACGTCTTCCACGCCGGCGACG
GCAACCTGCATCCGCTGATCCTGTTCGACGCCAACACCCCCGGCGAACTCGAGCGCGCCGAGGATTTCGGCGGCAGGATC
CTCGAGCTGTGCGTCGAAGCCGGCGGCACGATCACCGGCGAACACGGCGTCGGGCTCGAGAAAATCAAGCAGATGTGCGC
GCAGTTCGCGGCGCCCGAACTCACGCGCTTCCACGACGTGAAGGCCGCGTTCGATCCCGAGCGCCTGCTCAATCCGGGCA
AGGCCGTCCCGGAGCTGCACCGTTGCGCCGAATGGGGCGCGATGCACGTGCACGCGGGGCAGTTGCCGCACCCCGAACTG
CCGAGGTTTTGA

Upstream 100 bases:

>100_bases
CCGGGCAACGTCGGCGGCGACGACGCCCTGGCGGAGGTCTACCGCCGCCTCGCCCCCTCGCGCGCGCAGGCGGGCCGGGC
TAAACTCGCTGCCTGACTTT

Downstream 100 bases:

>100_bases
TGCCCGACGCGCTAATCGAACGCGTTCGCGCGGCCTACGAGAGCGGTACGCCGCTCGTGATCCAGGGCAGCGGTAGCAAG
GCCTTCTACGGCAACGCGGG

Product: glycolate oxidase subunit GlcD

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MLSPSFLAHLRTLLPAHCVLNEREDLRPFESDGLAAYRNTPDVVVLPETEAQVAAILELCHAHRVAVVARGAGTGLSAGA
LPVDGAVLLVLAKLNRIKAIDPLARTAVVEPGVRNLAISEAAAAYNLYYAPDPSSQIACSIGGNVAENSGGVHCLKYGLT
VHNIAKLRAWTVDGKLLEIGSDALDAPGYDLLALLTGSEGMLAVITEVTVKLLPRPERAQVVLAAFDDVAKAGAAVGNII
AAGVVPAGLEMMDRLAIQAAEAFVHAGYPMAEALLLCEVDGVNEEVSADIYTVRQVLEASGATEIRTADSEEQRLRFWAG
RKAAFPAVGRLAPDYYCMDGTIPRAQLPHVLQRIGALSAEFGLAVANVFHAGDGNLHPLILFDANTPGELERAEDFGGRI
LELCVEAGGTITGEHGVGLEKIKQMCAQFAAPELTRFHDVKAAFDPERLLNPGKAVPELHRCAEWGAMHVHAGQLPHPEL
PRF

Sequences:

>Translated_483_residues
MLSPSFLAHLRTLLPAHCVLNEREDLRPFESDGLAAYRNTPDVVVLPETEAQVAAILELCHAHRVAVVARGAGTGLSAGA
LPVDGAVLLVLAKLNRIKAIDPLARTAVVEPGVRNLAISEAAAAYNLYYAPDPSSQIACSIGGNVAENSGGVHCLKYGLT
VHNIAKLRAWTVDGKLLEIGSDALDAPGYDLLALLTGSEGMLAVITEVTVKLLPRPERAQVVLAAFDDVAKAGAAVGNII
AAGVVPAGLEMMDRLAIQAAEAFVHAGYPMAEALLLCEVDGVNEEVSADIYTVRQVLEASGATEIRTADSEEQRLRFWAG
RKAAFPAVGRLAPDYYCMDGTIPRAQLPHVLQRIGALSAEFGLAVANVFHAGDGNLHPLILFDANTPGELERAEDFGGRI
LELCVEAGGTITGEHGVGLEKIKQMCAQFAAPELTRFHDVKAAFDPERLLNPGKAVPELHRCAEWGAMHVHAGQLPHPEL
PRF
>Mature_483_residues
MLSPSFLAHLRTLLPAHCVLNEREDLRPFESDGLAAYRNTPDVVVLPETEAQVAAILELCHAHRVAVVARGAGTGLSAGA
LPVDGAVLLVLAKLNRIKAIDPLARTAVVEPGVRNLAISEAAAAYNLYYAPDPSSQIACSIGGNVAENSGGVHCLKYGLT
VHNIAKLRAWTVDGKLLEIGSDALDAPGYDLLALLTGSEGMLAVITEVTVKLLPRPERAQVVLAAFDDVAKAGAAVGNII
AAGVVPAGLEMMDRLAIQAAEAFVHAGYPMAEALLLCEVDGVNEEVSADIYTVRQVLEASGATEIRTADSEEQRLRFWAG
RKAAFPAVGRLAPDYYCMDGTIPRAQLPHVLQRIGALSAEFGLAVANVFHAGDGNLHPLILFDANTPGELERAEDFGGRI
LELCVEAGGTITGEHGVGLEKIKQMCAQFAAPELTRFHDVKAAFDPERLLNPGKAVPELHRCAEWGAMHVHAGQLPHPEL
PRF

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=422, Percent_Identity=29.8578199052133, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI37595754, Length=445, Percent_Identity=28.314606741573, Blast_Score=181, Evalue=9e-46,
Organism=Homo sapiens, GI119964728, Length=448, Percent_Identity=27.0089285714286, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI4501993, Length=252, Percent_Identity=25.7936507936508, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1789351, Length=480, Percent_Identity=66.25, Blast_Score=650, Evalue=0.0,
Organism=Escherichia coli, GI1787977, Length=500, Percent_Identity=24.4, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI48994907, Length=499, Percent_Identity=22.2444889779559, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71992373, Length=464, Percent_Identity=27.3706896551724, Blast_Score=184, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI71992381, Length=464, Percent_Identity=26.5086206896552, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI71992392, Length=370, Percent_Identity=27.5675675675676, Blast_Score=157, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI17534361, Length=460, Percent_Identity=25.8695652173913, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17556096, Length=217, Percent_Identity=24.4239631336406, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320027, Length=428, Percent_Identity=26.6355140186916, Blast_Score=166, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6320023, Length=487, Percent_Identity=26.694045174538, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6320764, Length=482, Percent_Identity=26.1410788381743, Blast_Score=152, Evalue=1e-37,
Organism=Drosophila melanogaster, GI18921117, Length=468, Percent_Identity=27.5641025641026, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24639277, Length=468, Percent_Identity=27.5641025641026, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24639275, Length=468, Percent_Identity=27.5641025641026, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24653753, Length=231, Percent_Identity=25.5411255411255, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 51245; Mature: 51245

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSPSFLAHLRTLLPAHCVLNEREDLRPFESDGLAAYRNTPDVVVLPETEAQVAAILELC
CCCHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCEEECCCCCEEEECCCHHHHHHHHHHH
HAHRVAVVARGAGTGLSAGALPVDGAVLLVLAKLNRIKAIDPLARTAVVEPGVRNLAISE
HHHCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHH
AAAAYNLYYAPDPSSQIACSIGGNVAENSGGVHCLKYGLTVHNIAKLRAWTVDGKLLEIG
HHHHEEEEECCCCCCCEEEEECCCCCCCCCCEEEEEECCHHHHHHHHEEEEECCEEEECC
SDALDAPGYDLLALLTGSEGMLAVITEVTVKLLPRPERAQVVLAAFDDVAKAGAAVGNII
CCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHEEEEHHHHHHHHHHHHHHHH
AAGVVPAGLEMMDRLAIQAAEAFVHAGYPMAEALLLCEVDGVNEEVSADIYTVRQVLEAS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEECCCCHHHHHHHHHHHHHHHHC
GATEIRTADSEEQRLRFWAGRKAAFPAVGRLAPDYYCMDGTIPRAQLPHVLQRIGALSAE
CCCEEECCCCHHHHHHHHCCCCCCCCHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCHHH
FGLAVANVFHAGDGNLHPLILFDANTPGELERAEDFGGRILELCVEAGGTITGEHGVGLE
HHHHHHHHEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCHH
KIKQMCAQFAAPELTRFHDVKAAFDPERLLNPGKAVPELHRCAEWGAMHVHAGQLPHPEL
HHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHCCCEEEECCCCCCCCC
PRF
CCC
>Mature Secondary Structure
MLSPSFLAHLRTLLPAHCVLNEREDLRPFESDGLAAYRNTPDVVVLPETEAQVAAILELC
CCCHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCEEECCCCCEEEECCCHHHHHHHHHHH
HAHRVAVVARGAGTGLSAGALPVDGAVLLVLAKLNRIKAIDPLARTAVVEPGVRNLAISE
HHHCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHH
AAAAYNLYYAPDPSSQIACSIGGNVAENSGGVHCLKYGLTVHNIAKLRAWTVDGKLLEIG
HHHHEEEEECCCCCCCEEEEECCCCCCCCCCEEEEEECCHHHHHHHHEEEEECCEEEECC
SDALDAPGYDLLALLTGSEGMLAVITEVTVKLLPRPERAQVVLAAFDDVAKAGAAVGNII
CCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHEEEEHHHHHHHHHHHHHHHH
AAGVVPAGLEMMDRLAIQAAEAFVHAGYPMAEALLLCEVDGVNEEVSADIYTVRQVLEAS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEECCCCHHHHHHHHHHHHHHHHC
GATEIRTADSEEQRLRFWAGRKAAFPAVGRLAPDYYCMDGTIPRAQLPHVLQRIGALSAE
CCCEEECCCCHHHHHHHHCCCCCCCCHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCHHH
FGLAVANVFHAGDGNLHPLILFDANTPGELERAEDFGGRILELCVEAGGTITGEHGVGLE
HHHHHHHHEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCHH
KIKQMCAQFAAPELTRFHDVKAAFDPERLLNPGKAVPELHRCAEWGAMHVHAGQLPHPEL
HHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHCCCEEEECCCCCCCCC
PRF
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]