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The map label for this gene is glcE [H]

Identifier: 74316064

GI number: 74316064

Start: 46631

End: 47677

Strand: Reverse

Name: glcE [H]

Synonym: Tbd_0046

Alternate gene names: 74316064

Gene position: 47677-46631 (Counterclockwise)

Preceding gene: 74316065

Following gene: 74316063

Centisome position: 1.64

GC content: 73.83

Gene sequence:

>1047_bases
ATGCCCGACGCGCTAATCGAACGCGTTCGCGCGGCCTACGAGAGCGGTACGCCGCTCGTGATCCAGGGCAGCGGTAGCAA
GGCCTTCTACGGCAACGCGGGCGGCGGCGAGATCCTCGACACGCGGGGCGTCGCCGGCATCGTCGACTACCAGCCGAAGG
AGCTCGTGCTGACCGCGCGCGCCGGCACGCCCCTCGCCGAGATCGAAACCCTGCTCGACGCGCACCAGCAAATGCTTGCC
TTCGAGCCGCCGCGCTTCGGCGGCGCGGGCACCCTCGGCGGCAGCGTCGCGGCCGGGCTTTCGGGTCCACGCCGGCCCTA
CGCCGGTGCCGCGCGCGACTTCGTGCTCGGCCTGCGCCTGATCGACGGCACCGGGCAGGCGCTGCGCTTCGGCGGCCAGG
TCATCAAGAACGTCGCCGGCTACGACGTCTCGCGGCTGATGGTCGGCGCGCTCGGCACGCTCGGCGTCGTCACCGAGGTG
TCGCTCAAGGTGCTGCCACGCCCGGCGAGCGAACTGACGCTGCAATTCGAATTCGACGAAGCCGCCGCGCTGCGCGCGAT
GAACCAGTGGGCGGGGCTGCCCTTGCCGCTCTCGGCAAGCTCGTGGCACGCGGGGCTGCTGACGCTGCGGCTCTCCGGAG
CGGCCACCGCGGTGCGCGCCGCGCACGCCAGACTCGGCGGTGAGACGATCGAGGATGCGGCGGCGTTCTGGCAGCGGCTG
CGCGACCACGCGACCCCCTTCTTCGACAAGCGCCCGCTCTGGCGTCTGGCGACCGGGCCGACCGCGGCGCCGATCGGCCT
CGACGCGCCGCACTGGATCGAGTGGGGCGGCGCGGTCCGCTGGCTCGCCACCGACCTGCCGGCTGCCGCACTGCGGCGCG
CGGCGCAGGCGGCCGGCGGGCACGCGACGCTGTTCCGCGGCGACGCTCCGCTCGACGGCGTTTTCACGCCGCTCGCGCCC
GCGCTCGCGACGCTGCACGCCCGCCTCAAGCAACGCTTCGACCCGCGCGGCATCCTGAACCGCGGCCGCCTGTACCCGGA
CTTCTGA

Upstream 100 bases:

>100_bases
ATCCGGGCAAGGCCGTCCCGGAGCTGCACCGTTGCGCCGAATGGGGCGCGATGCACGTGCACGCGGGGCAGTTGCCGCAC
CCCGAACTGCCGAGGTTTTG

Downstream 100 bases:

>100_bases
ATGGATATCGTCTCGATTGACCACGTGGTGCTGACCGTCCGCGACATCCGCGCGAGCGTGGCCTTTTACACGCGGGTGCT
GGGCATGCAGGAGGTGATGT

Product: glycolate oxidase FAD binding subunit

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MPDALIERVRAAYESGTPLVIQGSGSKAFYGNAGGGEILDTRGVAGIVDYQPKELVLTARAGTPLAEIETLLDAHQQMLA
FEPPRFGGAGTLGGSVAAGLSGPRRPYAGAARDFVLGLRLIDGTGQALRFGGQVIKNVAGYDVSRLMVGALGTLGVVTEV
SLKVLPRPASELTLQFEFDEAAALRAMNQWAGLPLPLSASSWHAGLLTLRLSGAATAVRAAHARLGGETIEDAAAFWQRL
RDHATPFFDKRPLWRLATGPTAAPIGLDAPHWIEWGGAVRWLATDLPAAALRRAAQAAGGHATLFRGDAPLDGVFTPLAP
ALATLHARLKQRFDPRGILNRGRLYPDF

Sequences:

>Translated_348_residues
MPDALIERVRAAYESGTPLVIQGSGSKAFYGNAGGGEILDTRGVAGIVDYQPKELVLTARAGTPLAEIETLLDAHQQMLA
FEPPRFGGAGTLGGSVAAGLSGPRRPYAGAARDFVLGLRLIDGTGQALRFGGQVIKNVAGYDVSRLMVGALGTLGVVTEV
SLKVLPRPASELTLQFEFDEAAALRAMNQWAGLPLPLSASSWHAGLLTLRLSGAATAVRAAHARLGGETIEDAAAFWQRL
RDHATPFFDKRPLWRLATGPTAAPIGLDAPHWIEWGGAVRWLATDLPAAALRRAAQAAGGHATLFRGDAPLDGVFTPLAP
ALATLHARLKQRFDPRGILNRGRLYPDF
>Mature_347_residues
PDALIERVRAAYESGTPLVIQGSGSKAFYGNAGGGEILDTRGVAGIVDYQPKELVLTARAGTPLAEIETLLDAHQQMLAF
EPPRFGGAGTLGGSVAAGLSGPRRPYAGAARDFVLGLRLIDGTGQALRFGGQVIKNVAGYDVSRLMVGALGTLGVVTEVS
LKVLPRPASELTLQFEFDEAAALRAMNQWAGLPLPLSASSWHAGLLTLRLSGAATAVRAAHARLGGETIEDAAAFWQRLR
DHATPFFDKRPLWRLATGPTAAPIGLDAPHWIEWGGAVRWLATDLPAAALRRAAQAAGGHATLFRGDAPLDGVFTPLAPA
LATLHARLKQRFDPRGILNRGRLYPDF

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Escherichia coli, GI48994914, Length=349, Percent_Identity=49.5702005730659, Blast_Score=317, Evalue=5e-88,
Organism=Saccharomyces cerevisiae, GI6320027, Length=236, Percent_Identity=22.4576271186441, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 36758; Mature: 36626

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDALIERVRAAYESGTPLVIQGSGSKAFYGNAGGGEILDTRGVAGIVDYQPKELVLTAR
CCHHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCCCEEECCCCEEEECCCCCEEEEEEC
AGTPLAEIETLLDAHQQMLAFEPPRFGGAGTLGGSVAAGLSGPRRPYAGAARDFVLGLRL
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHEEEEE
IDGTGQALRFGGQVIKNVAGYDVSRLMVGALGTLGVVTEVSLKVLPRPASELTLQFEFDE
ECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECCCCHHHEEEEEECCH
AAALRAMNQWAGLPLPLSASSWHAGLLTLRLSGAATAVRAAHARLGGETIEDAAAFWQRL
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHH
RDHATPFFDKRPLWRLATGPTAAPIGLDAPHWIEWGGAVRWLATDLPAAALRRAAQAAGG
HHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEHHHCCCHHHHHHHHHHCCC
HATLFRGDAPLDGVFTPLAPALATLHARLKQRFDPRGILNRGRLYPDF
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCC
>Mature Secondary Structure 
PDALIERVRAAYESGTPLVIQGSGSKAFYGNAGGGEILDTRGVAGIVDYQPKELVLTAR
CHHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCCCEEECCCCEEEECCCCCEEEEEEC
AGTPLAEIETLLDAHQQMLAFEPPRFGGAGTLGGSVAAGLSGPRRPYAGAARDFVLGLRL
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHEEEEE
IDGTGQALRFGGQVIKNVAGYDVSRLMVGALGTLGVVTEVSLKVLPRPASELTLQFEFDE
ECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECCCCHHHEEEEEECCH
AAALRAMNQWAGLPLPLSASSWHAGLLTLRLSGAATAVRAAHARLGGETIEDAAAFWQRL
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHH
RDHATPFFDKRPLWRLATGPTAAPIGLDAPHWIEWGGAVRWLATDLPAAALRRAAQAAGG
HHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEHHHCCCHHHHHHHHHHCCC
HATLFRGDAPLDGVFTPLAPALATLHARLKQRFDPRGILNRGRLYPDF
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]