Click here to switch to the map view.

The map label for this gene is dsbE [H]

Identifier: 73538596

GI number: 73538596

Start: 1471718

End: 1472257

Strand: Direct

Name: dsbE [H]

Synonym: Reut_B4771

Alternate gene names: 73538596

Gene position: 1471718-1472257 (Clockwise)

Preceding gene: 73538595

Following gene: 73538597

Centisome position: 53.99

GC content: 67.78

Gene sequence:

>540_bases
ATGACGCGCTTCCTGCTGCCGCTTGCGGTGTTCCTGGCGCTCGCTGTGGCGCTGGCCGCGGGCTTGCGCCACGATCCGCG
CGAGCTCCCGTCGCCACTGGTCGGCAAGCCCGCGCCAGCATTCGTGCTGCCGGTGCTTGACGAGGACGGCCAGAAGCTCG
GCGCGGCTGATCTGCGCGGCAAGGTGTGGTTGCTCAATGTCTGGGCATCGTGGTGCACAGCGTGCCGCGCTGAGCATCCG
GTGCTCGTCGACTTCGCGGCGCGCTCGCGCGTGCCGCTGTACGGGCTCAACTACAAGGATCAGGACAGCGCCGCGCGCGA
CTGGCTGCGGCGCATGGGCAATCCGTATGCGGCGTCGCTGGTCGATGCCGACGGGCGCGTGGGCATCGACTTCGGCGTGT
ATGGCGTGCCGGAGACCTTTGTTATCGACAAGGCCGGCGTGATTCGCTACAAGCAGGTCGGTCCGGTGACGCAGGAGGTG
CTGGAGCGCAAGCTGATCCCGTTGCTGGAGTCTCTGCAGAAGGGGGACGGCCATGCGTAG

Upstream 100 bases:

>100_bases
GGCTGCACCGGCAGGCTGCCAGCGCTGTGGCACCGGAAGCGAATGTCCCCCAGCCTCAGGCGCCCGCACTGGCGCTCGCT
GGCAGCGAGGAGACGCAGGC

Downstream 100 bases:

>100_bases
GCCGACGCTCGCGTGCTGCGTGCTGGCGCTCTCGCTGCGTGCCATTGCCGCACCGCTCACCGACGCAGAACTCGACGCAC
GCGTCCACGCGCTCTCCGAG

Product: periplasmic protein thiol:disulfide oxidoreductase DsbE

Products: NA

Alternate protein names: Cytochrome c biogenesis protein CcmG [H]

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MTRFLLPLAVFLALAVALAAGLRHDPRELPSPLVGKPAPAFVLPVLDEDGQKLGAADLRGKVWLLNVWASWCTACRAEHP
VLVDFAARSRVPLYGLNYKDQDSAARDWLRRMGNPYAASLVDADGRVGIDFGVYGVPETFVIDKAGVIRYKQVGPVTQEV
LERKLIPLLESLQKGDGHA

Sequences:

>Translated_179_residues
MTRFLLPLAVFLALAVALAAGLRHDPRELPSPLVGKPAPAFVLPVLDEDGQKLGAADLRGKVWLLNVWASWCTACRAEHP
VLVDFAARSRVPLYGLNYKDQDSAARDWLRRMGNPYAASLVDADGRVGIDFGVYGVPETFVIDKAGVIRYKQVGPVTQEV
LERKLIPLLESLQKGDGHA
>Mature_178_residues
TRFLLPLAVFLALAVALAAGLRHDPRELPSPLVGKPAPAFVLPVLDEDGQKLGAADLRGKVWLLNVWASWCTACRAEHPV
LVDFAARSRVPLYGLNYKDQDSAARDWLRRMGNPYAASLVDADGRVGIDFGVYGVPETFVIDKAGVIRYKQVGPVTQEVL
ERKLIPLLESLQKGDGHA

Specific function: Involved in disulfide bond formation. Catalyzes a late, reductive step in the assembly of periplasmic c-type cytochromes, probably the reduction of disulfide bonds of the apocytochrome c to allow covalent linkage with the heme. Possible subunit of a heme

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein; Periplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1788523, Length=177, Percent_Identity=48.0225988700565, Blast_Score=156, Evalue=9e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004799
- InterPro:   IPR013740
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF08534 Redoxin [H]

EC number: NA

Molecular weight: Translated: 19555; Mature: 19424

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRFLLPLAVFLALAVALAAGLRHDPRELPSPLVGKPAPAFVLPVLDEDGQKLGAADLRG
CCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCC
KVWLLNVWASWCTACRAEHPVLVDFAARSRVPLYGLNYKDQDSAARDWLRRMGNPYAASL
CEEHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEECCCCCCHHHHHHHHHHCCCCCEEEE
VDADGRVGIDFGVYGVPETFVIDKAGVIRYKQVGPVTQEVLERKLIPLLESLQKGDGHA
ECCCCCEEEEECCCCCCCEEEECCCCCEEEHHCCHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TRFLLPLAVFLALAVALAAGLRHDPRELPSPLVGKPAPAFVLPVLDEDGQKLGAADLRG
CHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCC
KVWLLNVWASWCTACRAEHPVLVDFAARSRVPLYGLNYKDQDSAARDWLRRMGNPYAASL
CEEHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEECCCCCCHHHHHHHHHHCCCCCEEEE
VDADGRVGIDFGVYGVPETFVIDKAGVIRYKQVGPVTQEVLERKLIPLLESLQKGDGHA
ECCCCCEEEEECCCCCCCEEEECCCCCEEEHHCCHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]